Title:
BASE EDITING SYSTEM AND METHOD
Document Type and Number:
WIPO Patent Application WO/2019/041296
Kind Code:
A1
Abstract:
A base editing system and method constructed based on a CRISPR/Cpf1 system are provided. The base editing system utilizes Cpf1 to recognize features of a 5'-TTTN-3' (PAM) sequence, and carries out base editing in regions rich in adenine and thymine in genomic DNA. The base editing system comprises at least one of a Cpf1-BE0 expression vector, a Cpf1-BE expression vector, a Cpf1-BE-YE expression vector, a Cpf1-BE-UGI expression vector, an RNA product expressed by the Cpf1-BE0 expression vector, an RNA product expressed by the Cpf1-BE expression vector, an RNA product expressed by the Cpf1-BE-YE expression vector, an RNA product expressed by the Cpf1-BE-UGI expression vector, a protein product expressed by the Cpf1-BE0 expression vector, a protein product expressed by the Cpf1-BE expression vector, a protein product expressed by the Cpf1-BE-YE expression vector, and a protein product expressed by the Cpf1-BE-UGI expression vector. A method for carrying out base editing in the genome of a eukaryote is also provided.
Inventors:
CHEN JIA (CN)
HUANG XINGXU (CN)
YANG LI (CN)
YANG BEI (CN)
LI XIAOSA (CN)
WANG YING (CN)
LIU YAJING (CN)
HUANG XINGXU (CN)
YANG LI (CN)
YANG BEI (CN)
LI XIAOSA (CN)
WANG YING (CN)
LIU YAJING (CN)
Application Number:
PCT/CN2017/100131
Publication Date:
March 07, 2019
Filing Date:
September 01, 2017
Export Citation:
Assignee:
UNIV SHANGHAI TECH (CN)
International Classes:
C12N15/79
Foreign References:
| US20170121693A1 | 2017-05-04 | |||
| US20170233756A1 | 2017-08-17 | |||
| CN105907785A | 2016-08-31 | |||
| CN106282228A | 2017-01-04 |
Other References:
ZETSCHE: "Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR- Cas System", CELL, vol. 163, no. 3, 25 September 2015 (2015-09-25), pages 759 - 771, XP055553375, DOI: 10.1016/j.cell.2015.09.038
WANG: "Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor", CELL RESEARCH, vol. 27, no. 10, 29 August 2017 (2017-08-29), pages 1289 - 1292, XP055579596, DOI: 10.1038/cr.2017.111
MAKAROVA: "Snapshot: Class 2 CRISPR-Cas Systems", CELL, vol. 168, no. 1, 12 January 2017 (2017-01-12), pages 328, XP029882153, DOI: 10.1016/j.cell.2016.12.038
STELLA: "Structure of the Cpfl endonuclease R-loop complex after target DNA cleavage", NATURE, vol. 546, no. 7659, 31 May 2017 (2017-05-31), pages 559 - 563, XP002789283, DOI: 10.1038/nature22398
WANG: "Enhanced base editing by co-expression of free uracil DNA glycosylase inhibitor", CELL RESEARCH, vol. 27, no. 10, 29 August 2017 (2017-08-29), pages 1289 - 1292, XP055579596, DOI: 10.1038/cr.2017.111
MAKAROVA: "Snapshot: Class 2 CRISPR-Cas Systems", CELL, vol. 168, no. 1, 12 January 2017 (2017-01-12), pages 328, XP029882153, DOI: 10.1016/j.cell.2016.12.038
STELLA: "Structure of the Cpfl endonuclease R-loop complex after target DNA cleavage", NATURE, vol. 546, no. 7659, 31 May 2017 (2017-05-31), pages 559 - 563, XP002789283, DOI: 10.1038/nature22398
Attorney, Agent or Firm:
SHANGHAI SHENHUI PATENT AGENT CO., LTD. (CN)
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