Title:
Beta-galactosidase isolated from a bifidobacterium
Document Type and Number:
WIPO Patent Application WO2001090317
Kind Code:
A3
Abstract:
The present invention concerns a new beta-galactosidase with transgalactosylating activity isolated from Bifidobacterium bifidum and a truncated enzyme where the C-terminal end of the beta-galactosidase protein has been deleted, resulting in an enzyme with a higher transgalactosylating activity than hydrolase activity. When lactose is used as a substrate, galacto-oligosaccharides are products of the transgalactosylase activity. Galacto-oligosaccharides enhance growth of health-promoting Bifidobacterium that may be used in a number of applications in the dairy industry.
Inventors:
JORGENSEN FLEMMING (DK)
HANSEN OLEN (DK)
STOUGAARD PETER (DK)
HANSEN OLEN (DK)
STOUGAARD PETER (DK)
Application Number:
PCT/DK2001/000366
Publication Date:
April 25, 2002
Filing Date:
May 25, 2001
Export Citation:
Assignee:
ARLA FOODS AMBA (DK)
International Classes:
A23C9/127; A23C19/032; A23L33/00; C12N1/15; C12N1/19; C12N1/20; C12N1/21; C12N9/10; C12N9/38; C12N15/09; C12P19/18; C12R1/01; (IPC1-7): C12N9/38
Foreign References:
| US5153128A | 1992-10-06 |
Other References:
VINCENT DUMORTIER ET AL: "Purification and properties of a beta-D-galactosidase from Bifidobacterium bifidum exhibiting a transgalactosylation reaction 1", BIOTECHNOL. APPL. BIOCHEM, vol. 19, 1994, XP002902258
J GARMAN ET AL: "The effect of cations on the hydrolysis of lactose and the transferase reactions catalysed by beta-galactosidase from six strains of lactic acid bacteria", APPL. MICROBIOL BIOTECHNOL., vol. 46, 1996, XP002902259
DATABASE WPI Section Ch Week 199328, Derwent World Patents Index; Class B04, AN 1993-223533, XP002905667
J GARMAN ET AL: "The effect of cations on the hydrolysis of lactose and the transferase reactions catalysed by beta-galactosidase from six strains of lactic acid bacteria", APPL. MICROBIOL BIOTECHNOL., vol. 46, 1996, XP002902259
DATABASE WPI Section Ch Week 199328, Derwent World Patents Index; Class B04, AN 1993-223533, XP002905667
Download PDF:
Previous Patent: HUMAN CIRCULATING DENDRITIC CELL COMPOSITIONS AND METHODS
Next Patent: REGULATION OF HUMAN POLYAMINE OXIDASE-LIKE ENZYME
Next Patent: REGULATION OF HUMAN POLYAMINE OXIDASE-LIKE ENZYME