Title:
BIMOLECULAR FLUORESCENCE COMPLEMENTARY AMINO ACID SEQUENCE PAIR FOR MONITORING INTERACTION OF INTRACELLULAR TAU PROTEIN
Document Type and Number:
WIPO Patent Application WO/2015/053439
Kind Code:
A1
Abstract:
The present invention relates to a protein pair for monitoring an interaction between tau proteins in living cells, a base sequence pair encoding the same, a vector pair comprising the same, cell lines transformed by the vector pair, and a method for monitoring tau-tau interaction in living cells using the cell lines, and, more specifically, to a bimolecular fluorescence complementary amino acid sequence pair for monitoring an interaction of intracellular tau proteins, a base sequence encoding the same, a vector pair comprising the same, cell lines transformed by the vector pair, and a method for monitoring tau-tau interaction in living cells using the cell lines, in which the bimolecular fluorescence complementary amino acid sequence pair consists of: a first fluorescence complementary amino acid sequence in which the N-terminal sequence of a Venus amino acid sequence is bound to the C-terminal of a full-length human tau amino acid sequence; and a second fluorescence complementary amino acid sequence in which the C-terminal sequence of the Venus amino acid sequence is bound to the C-terminal of the full-length human tau amino acid sequence. The present invention can monitor and quantify a tau oligomerization process by directly visualizing tau-tau interaction in living cells, and thus may be used as a useful means to investigate the occurrence of tau-associated diseases and to develop a method for preventing and reversing the oligomerization process.
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Inventors:
KIM YUN KYUNG (KR)
KIM DONG JIN (KR)
PARK KI DUK (KR)
TAK HYE JIN (KR)
GRAILHE REGIS (KR)
MIN SUN-JOON (KR)
PAE AE NIM (KR)
KIM YOUNGSOO (KR)
KIM DONG JIN (KR)
PARK KI DUK (KR)
TAK HYE JIN (KR)
GRAILHE REGIS (KR)
MIN SUN-JOON (KR)
PAE AE NIM (KR)
KIM YOUNGSOO (KR)
Application Number:
PCT/KR2014/001214
Publication Date:
April 16, 2015
Filing Date:
February 14, 2014
Export Citation:
Assignee:
KOREA INST SCI & TECH (KR)
International Classes:
C07K14/47; C12N15/12; C12N15/63
Foreign References:
| KR20130091288A | 2013-08-16 | |||
| KR101050669B1 | 2011-07-19 |
Other References:
SHYU, Y. JOHN ET AL.: "Identification of new fluorescent protein fragments for bimolecula fluorescence complementation analysis under physiological conditions", BIOTECHNIQUES, vol. 40, no. 1, 2006, pages 61 - 66, XP001536504, DOI: doi:10.2144/000112036
KERPPOLA, TOM K.: "Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells", ANNUAL REVIEW OF BIOPHYSICS, vol. 37, 2008, pages 465 - 487, XP055065255, DOI: doi:10.1146/annurev.biophys.37.032807.125842
KODAMA, YUTAKA ET AL.: "An improved bimolecular fluorescence complementation assay with a high signal-to-noise ratio", BIOTECHNIQUES, vol. 49, no. 5, 2010, pages 793 - 805
TAK, HYEJIN ET AL.: "Bimolecular fluorescence complementation; lighting-up Tau-Tau interaction in living cells", PLOS ONE, vol. 8, no. 12, December 2013 (2013-12-01), pages 1 - 7
KERPPOLA, TOM K.: "Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells", ANNUAL REVIEW OF BIOPHYSICS, vol. 37, 2008, pages 465 - 487, XP055065255, DOI: doi:10.1146/annurev.biophys.37.032807.125842
KODAMA, YUTAKA ET AL.: "An improved bimolecular fluorescence complementation assay with a high signal-to-noise ratio", BIOTECHNIQUES, vol. 49, no. 5, 2010, pages 793 - 805
TAK, HYEJIN ET AL.: "Bimolecular fluorescence complementation; lighting-up Tau-Tau interaction in living cells", PLOS ONE, vol. 8, no. 12, December 2013 (2013-12-01), pages 1 - 7
Attorney, Agent or Firm:
CHUNG HYUN PATENT & LAW FIRM (KR)
특허법인 충현 (KR)
특허법인 충현 (KR)
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