Title:
BIOCHIP SUBSTRATUM AND METHOD FOR PRODUCTION THEREOF
Document Type and Number:
WIPO Patent Application WO/2010/001876
Kind Code:
A1
Abstract:
Disclosed is a biochip substratum for immobilizing thereon a probe comprising a biological substance (e.g., a protein, a nucleic acid), wherein the biochip substratum can immobilize the probe at a high immobilization ratio and a uniform immobilization density, and can prevent the non-specific adsorption of a protein to thereby achieve high detection sensitivity and high reproducibility. A polymer containing an amino group is immobilized on the surface of a substratum via a covalent bond, thereby attaching the amino group to the surface of the substratum uniformly, at a high density and stably. By utilizing the amino group, a probe comprising a biological substance (e.g., a protein, a nucleic acid) can be immobilized. In this manner, it becomes possible to immobilize the probe at a high immobilization ratio and a uniform immobilization density, and to prevent the non-specific adsorption of a protein to thereby achieve high detection sensitivity and high reproducibility.
Inventors:
NOKIHARA KIYOSHI (JP)
HIRATA AKIYOSHI (JP)
OKA YASUO (JP)
TAKEBAYASHI YASUSHI (JP)
HIRATA AKIYOSHI (JP)
OKA YASUO (JP)
TAKEBAYASHI YASUSHI (JP)
Application Number:
PCT/JP2009/061908
Publication Date:
January 07, 2010
Filing Date:
June 30, 2009
Export Citation:
Assignee:
HIPEP LAB (JP)
NIPPON LIGHT METAL CO (JP)
NOKIHARA KIYOSHI (JP)
HIRATA AKIYOSHI (JP)
OKA YASUO (JP)
TAKEBAYASHI YASUSHI (JP)
NIPPON LIGHT METAL CO (JP)
NOKIHARA KIYOSHI (JP)
HIRATA AKIYOSHI (JP)
OKA YASUO (JP)
TAKEBAYASHI YASUSHI (JP)
International Classes:
G01N33/543; C12N15/09; G01N33/545; G01N33/551; G01N37/00
Domestic Patent References:
WO2006059727A1 | 2006-06-08 | |||
WO2006126568A1 | 2006-11-30 |
Foreign References:
JP2004347317A | 2004-12-09 | |||
JP2005195465A | 2005-07-21 |
Other References:
See also references of EP 2306197A4
Attorney, Agent or Firm:
TANIGAWA, Hidejiro (JP)
Mountain stream Eijiro (JP)
Mountain stream Eijiro (JP)
Download PDF: