Title:
BIOCONVERSION PROCESS USING OLEIC ACID HYDRATASE 2
Document Type and Number:
WIPO Patent Application WO/2017/119576
Kind Code:
A1
Abstract:
The present invention relates to a bioconversion process for producing a heavy chain fatty acid and a heavy chain omega-hydroxy fatty acid from a long-chain unsaturated fatty acid substrate by using a novel oleic acid hydratase 2; a novel oleic acid hydratase 2 used for the process; a polynucleotide for coding the oleic acid hydratase 2; and a method for increasing the proportion of water-soluble expression to the total expression level of a target protein. When using the oleic acid hydratase 2 and the method for increasing the expression proportion of a water-soluble protein provided by the present invention, it is possible to increase the efficiency of a conventional bioconversion process for producing a heavy chain fatty acid and a heavy chain omega-hydroxy fatty acid from an unsaturated long chain fatty acid, and therefore the present invention can be widely used for the development of a more effective bioconversion process.
Inventors:
PARK JIN BYUNG (KR)
OH DEOK KUN (KR)
SONG JI WON (KR)
LEE JUNG HOO (KR)
JEON EUN-YEUNG (KR)
KANG WOO RI (KR)
OH DEOK KUN (KR)
SONG JI WON (KR)
LEE JUNG HOO (KR)
JEON EUN-YEUNG (KR)
KANG WOO RI (KR)
Application Number:
PCT/KR2016/011224
Publication Date:
July 13, 2017
Filing Date:
October 07, 2016
Export Citation:
Assignee:
EWHA UNIV - IND COLLABORATION FOUND (KR)
UNIV KONKUK IND COOP CORP (KR)
UNIV KONKUK IND COOP CORP (KR)
International Classes:
C12N9/88; C12N9/02; C12N15/52; C12N15/67; C12N15/70; C12P7/64
Foreign References:
| KR20130132254A | 2013-12-04 | |||
| KR20150081185A | 2015-07-13 | |||
| KR20130136381A | 2013-12-12 |
Other References:
DATABASE PROTEIN 31 May 2019 (2019-05-31), ANONYMOUS: "oleate hydratase [Stenotrophomonas maltophilia]", XP055598804, retrieved from NCBI Database accession no. WP_017356052
WENNBORG, ANDERS: "A human RNase E-like activity that cleaves RNA sequences involved in mRNA stability control", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA- BIOCHEMISTRY, vol. 92, no. 16, August 1995 (1995-08-01), pages 7322 - 7326, XP055598813, ISSN: 0027-8424, DOI: 10.1073/pnas.92.16.7322
LOPEZ-GARRIDO, JAVIER: "A eukaryotic-like 3' untranslated region in Salmonella enterica hilD mRNA", NUCLEIC ACIDS RESEARCH, vol. 42, no. 9, 20 March 2014 (2014-03-20) - 14 May 2014 (2014-05-14), pages 5894 - 5906, XP055598818, ISSN: 0305-1048, DOI: 10.1093/nar/gku222
SONG, JI-WON: "3' -UTR engineering to improve soluble expression and fine-tuning of activity of cascade enzymes in Escherichia coli", SCIENTIFIC REPORTS, vol. 6, no. 29406, 11 July 2016 (2016-07-11), pages 1 - 10
WENNBORG, ANDERS: "A human RNase E-like activity that cleaves RNA sequences involved in mRNA stability control", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA- BIOCHEMISTRY, vol. 92, no. 16, August 1995 (1995-08-01), pages 7322 - 7326, XP055598813, ISSN: 0027-8424, DOI: 10.1073/pnas.92.16.7322
LOPEZ-GARRIDO, JAVIER: "A eukaryotic-like 3' untranslated region in Salmonella enterica hilD mRNA", NUCLEIC ACIDS RESEARCH, vol. 42, no. 9, 20 March 2014 (2014-03-20) - 14 May 2014 (2014-05-14), pages 5894 - 5906, XP055598818, ISSN: 0305-1048, DOI: 10.1093/nar/gku222
SONG, JI-WON: "3' -UTR engineering to improve soluble expression and fine-tuning of activity of cascade enzymes in Escherichia coli", SCIENTIFIC REPORTS, vol. 6, no. 29406, 11 July 2016 (2016-07-11), pages 1 - 10
Attorney, Agent or Firm:
SON, Min (KR)
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