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Title:
BIOLOGICAL METHOD FOR PRODUCING CIS-5-HYDROXY-L-PIPECOLINIC ACID
Document Type and Number:
WIPO Patent Application WO/2013/187438
Kind Code:
A1
Abstract:
The present invention provides a method for producing cis-5-hydroxy-L-pipecolinic acid. In the production method according to the present invention, a genetically modified microorganism that is so modified as to produce cis-5-hydroxy-L-pipecolinic acid directly can be used. The present invention also provides the genetically modified microorganism. A preferred embodiment of the present invention is characterized in that a genetically modified microorganism having both DNA encoding a protein involved in the biosynthesis of L-pipecolinic acid and DNA encoding a protein having a hydroxylase activity for cis-position-5 in L-pipecolinic acid is cultured in a culture medium and cis-5-hydroxy-L-pipecolinic acid is collected from a culture solution.

Inventors:
FUJII TADASHI (JP)
TAMURA KEISUKE (JP)
Application Number:
PCT/JP2013/066218
Publication Date:
December 19, 2013
Filing Date:
June 12, 2013
Export Citation:
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Assignee:
MICROBIOPHARM JAPAN CO LTD (JP)
International Classes:
C12N15/00; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/02; C12N15/09; C12P13/24
Domestic Patent References:
WO2001048216A12001-07-05
WO2001048216A12001-07-05
WO2009139365A12009-11-19
Foreign References:
JP4516712B22010-08-04
Other References:
DATABASE GENBANK [online] 9 September 2013 (2013-09-09), EARL, A.M.: "HYPOTHETICAL PROTEIN HMPREF9336_02629 [SEGNILIPARUS RUGOSUS ATCC BAA-974]", XP003034297, Database accession no. EFV12517.2
EARL M. ASHLEE ET AL.: "High quality draft genome sequence of Segniliparus rugosus CDC 945T=(ATCC BAA-974T)", STAND. GENOMIC SCI., vol. 5, 2011, pages 389 - 397, XP055181792
KLEIN CHRISTIAN ET AL.: "A Simple Procedure for Selective Hydroxylation of L-Proline and L-Pipecolic Acid with Recombinantly Expressed Proline Hydroxylases", ADV. SYNTH. CATAL., vol. 353, 2011, pages 1375 - 1383, XP055181796
BIOSCI. BIOTECHNOL. BIOCHEM., vol. 66, no. 3, 2002, pages 622 - 627
BIOSCI. BIOTECHNOL. BIOCHEM., vol. 66, no. 9, 2002, pages 1981 - 1984
ADV. SYNTH. CATAL., vol. 353, 2011, pages 1375 - 1383
SAMBROOK ET AL.: "Molecular Cloning A Laboratory Manual", COLD SPRING HARBOR LABORATORY PRESS
"Hybridization of Nucleic Acid Immobilization on Solid Supports", ANALYTICAL BIOCHEMISTRY, vol. 138, 1984, pages 267 - 284
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
"Molecular Cloning A Laboratory Manual"
"Molecular Cloning A Laboratory Manual", 1987, article "Current Protocols in Molecular Biology"
See also references of EP 2873730A4
Attorney, Agent or Firm:
SIKs & Co. (JP)
Patent business corporation patent firm Sykes (JP)
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