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Title:
BIOLOGICAL PREPARATION METHOD FOR CHIRAL N-PROTECTION PERIDOL
Document Type and Number:
WIPO Patent Application WO/2014/180085
Kind Code:
A1
Abstract:
Provided is a biological preparation method for chiral N- protection peridol, in which N-protection piperidone is used as a substrate, and the substrate is subjected to an asymmetric reduction reaction in the presence of a reconstructed ketone reduction enzyme, a cofactor, and isopropyl alcohol to generate chiral N- protection peridol. The asymmetric reduction reaction takes place in an aqueous buffer having a pH value of 7.0 to 9.0 at the temperature of 25°C to 45°C, so as to successfully prepare chiral N- protection peridol having a high optical purity. The method has temperate reaction conditions, high reaction efficiency, and simple operations; especially, the concentration of the reaction substrate can be increased to 20%, so that the method has important and practical industrial application value.

Inventors:
JU, Xin (F-Floor 2, No.1 Guotai North RoadZhangjiagang, Jiangsu 0, 215600, CN)
GUO, Hao (F-Floor 2, No.1 Guotai North RoadZhangjiagang, Jiangsu 0, 215600, CN)
LI, Bin (F-Floor 2, No.1 Guotai North RoadZhangjiagang, Jiangsu 0, 215600, CN)
TANG, Yuanyuan (F-Floor 2, No.1 Guotai North RoadZhangjiagang, Jiangsu 0, 215600, CN)
Application Number:
CN2013/083267
Publication Date:
November 13, 2014
Filing Date:
September 11, 2013
Export Citation:
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Assignee:
ENZYMEWORKS, INC. (F-Floor 2, No.1 Guotai North RoadZhangjiagang, Jiangsu 0, 215600, CN)
International Classes:
C12P17/12
Domestic Patent References:
WO2012007965A1
Foreign References:
CN102925501A
CN102978249A
CN102341501A
CN103276027A
Other References:
LACHERETZ ROMAIN ET AL.: 'Daucus carota Mediated-Reduction of Cyclic 3-Oxo-amines' ORGANIC LETTERS vol. 11, no. 6, 12 February 2009, pages 1245 - 1248
TEWARI Y. B. ET AL.: 'A thermodynamic study of ketoreductase- catalyzed reactions 5. Reduction of substituted ketones in n-hexane' J. CHEM.THERMODYNAMICS vol. 40, 07 November 2007, pages 661 - 670
KIZAKI NORIYUKI ET AL.: 'Characterization of novel alcohol dehydrogenase of Devosia riboflavina involved in stereoselective reduction of 3-pyrrolidinone derivatives' JOURNAL OF MOLECULAR CATALYSIS B: ENZYMATIC vol. 51, 09 November 2007, pages 73 - 80
Attorney, Agent or Firm:
SUZHOU CREATOR PATENT & TRADEMARK AGENCY LTD. (No.93 Ganjiang West Road, Suzhou, Jiangsu 2, 215002, CN)
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