Title:
BIOLOGICAL PREPARATION METHOD OF (S)-3-METHYLAMINO-1-(2-THIENYL)-1-PROPYL ALCOHOL
Document Type and Number:
WIPO Patent Application WO/2014/094462
Kind Code:
A1
Abstract:
The present invention provides a biological preparation method of (S)-3-methylamino-1-(2-thienyl)-1-propyl alcohol, wherein 3-methylamino-1-(2-thienyl)-1-acetone is used as a substrate; under a condition that a biocatalyst ketoreductase, a cofactor and a cofactor regeneration system (comprising glucose and glucose dehydrogenase) exist, the substrate undergoes an asymmetric reduction reaction to generate the (S)-3-methylamino-1-(2-thienyl)-1-propyl alcohol; the asymmetric reduction reaction is carried out in a water-phase buffer solution with a pH of 6-8; and in a initial reaction system, concentration of the substrate is 100-150 mg/mL, and a mass ratio of the ketoreductase to the substrate is 1-2%. The present invention improves the concentration of the substrate and reduces enzyme usage.
Inventors:
LE YONGTANG (CN)
JU XIN (CN)
JU XIN (CN)
Application Number:
PCT/CN2013/083235
Publication Date:
June 26, 2014
Filing Date:
September 10, 2013
Export Citation:
Assignee:
ENZYMEWORKS INC (CN)
International Classes:
C12P17/00
Foreign References:
| US20080220484A1 | 2008-09-11 | |||
| CN101613672A | 2009-12-30 | |||
| CN101993828A | 2011-03-30 | |||
| CN102206686A | 2011-10-05 |
Other References:
LI, LINGLING ET AL.: "Regeneration of catalytic coenzyme of recombinant glucose dehydrogenase", JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY ( NATURE SCIENCE EDITION, vol. 38, no. 3, March 2010 (2010-03-01), pages 112 - 115 AND 132
Attorney, Agent or Firm:
SUZHOU CREATOR PATENT & TRADEMARK AGENCY LTD. (CN)
苏州创元专利商标事务所有限公司 (CN)
苏州创元专利商标事务所有限公司 (CN)
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