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Title:
BIOLOGICAL PREPARATION METHOD OF (S)-3-(DIMETHYLAMINO)-1-(THIOPHENE-2-RADICAL)-1-PROPYL ALCOHOL
Document Type and Number:
WIPO Patent Application WO/2015/014031
Kind Code:
A1
Abstract:
The present invention relates to a biological preparation method of (S)-3-(dimethylamino)-1-(thiophene-2-radical)-1-propyl alcohol. According to the method, (S)-3-(dimethylamino)-1-(thiophene-2-radical)-1-acetone or salt thereof is used as a substrate, and the substrate is subjected to asymmetric reduction reaction in the presence of biocatalyst, cofactor and hydrogen donor to produce (S)-3-(dimethylamino)-1-(thiophene-2-radical)-1-propyl alcohol. Particularly, the biocatalyst is a combination of ketoreductase (KRED) and glucose dehydrogenase, the hydrogen donor is glucose, and the asymmetric reduction reaction is conducted at pH 6.8-7.0 and 25ºC-35ºC. Compared with the biological method in the prior art, the present invention has higher processing stability, and is simpler, more efficient and safer. During the reaction, the generation of highly toxic organic solvents such as acetone can be avoided, the application principle of green chemistry is met, and industrialized application is facilitated.

Inventors:
TAO, Junhua (F-Floor 2, No.1 Guotai North RoadZhangjiagang, Jiangsu 0, 215600, CN)
LI, Bin (F-Floor 2, No.1 Guotai North RoadZhangjiagang, Jiangsu 0, 215600, CN)
LE, Yongtang (F-Floor 2, No.1 Guotai North RoadZhangjiagang, Jiangsu 0, 215600, CN)
Application Number:
CN2013/086008
Publication Date:
February 05, 2015
Filing Date:
October 25, 2013
Export Citation:
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Assignee:
ENZYMEWORKS, INC. (F-Floor 2, No.1 Guotai North RoadZhangjiagang, Jiangsu 0, 215600, CN)
International Classes:
C12P17/00
Foreign References:
CN103014088A
US20100151534A1
CN101784669A
CN101889081A
Attorney, Agent or Firm:
SUZHOU CREATOR PATENT & TRADEMARK AGENCY LTD. (No.93 Ganjiang West Road, Suzhou, Jiangsu 2, 215002, CN)
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