Title:
BIOLOGICAL SUBSTANCE QUANTITATION METHOD, PATHOLOGICAL DIAGNOSIS SUPPORT SYSTEM, AND PROGRAM
Document Type and Number:
WIPO Patent Application WO/2016/117466
Kind Code:
A1
Abstract:
Provided are: a biological substance quantitation method with which the expression level of a nucleoprotein in a specimen can be accurately quantitated; a pathological diagnosis support system; and a program. This biological substance quantitation method, in which a biological substance is quantitated, on the basis of the fluorescence of a fluorescent substance, from a specimen that has been stained using a staining reagent that includes fluorescent particles containing said fluorescent substance, is characterized by including an input step for inputting a fluorescent image that represents the expression of the biological substance in the specimen using a fluorescent bright spot, and a quantitation step for quantitating the expression level of the biological substance on the basis of the fluorescence of the fluorescent bright spot, wherein the biological substance is a nucleoprotein that is expressed in a cell nucleus, and the fluorescent particles bind to the biological substance via a primary antibody for which the biological substance serves as an antigen.
Inventors:
ISODA TAKESHI (JP)
FURUSAWA NAOKO (JP)
MOTOKUI YASUYUKI (JP)
GONDA KOHSUKE (JP)
OHUCHI NORIAKI (JP)
WATANABE MIKA (JP)
FURUSAWA NAOKO (JP)
MOTOKUI YASUYUKI (JP)
GONDA KOHSUKE (JP)
OHUCHI NORIAKI (JP)
WATANABE MIKA (JP)
Application Number:
PCT/JP2016/051116
Publication Date:
July 28, 2016
Filing Date:
January 15, 2016
Export Citation:
Assignee:
KONICA MINOLTA INC (JP)
International Classes:
G01N33/53; G01N33/48; G01N33/536
Domestic Patent References:
| WO2012029752A1 | 2012-03-08 | |||
| WO2015045962A1 | 2015-04-02 |
Other References:
HIDEKI GODA ET AL.: "Development of a Quantitative Diagnostic Method of Estrogen Receptor Expression Levels by Immunohistochemistry Using Fluorescent Nanoparticles", KONICA MINOLTA TECHNOLOGY REPORT, vol. 11, 2014, pages 68 - 72, XP009500133
TUOMINEN, V. ET AL.: "ImmunoRatio-F: image analysis of Ki-67 using cytokeratin immunofluorescence correction", LLTH EUROPEAN CONGRESS ON TELEPATHOLOGY AND 5TH INTERNATIONAL CONGRESS ON VIRTUAL MICROSCOPY, 8 June 2012 (2012-06-08), XP055404537
TUOMINEN, V. ET AL.: "ImmunoRatio-F: image analysis of Ki-67 using cytokeratin immunofluorescence correction", LLTH EUROPEAN CONGRESS ON TELEPATHOLOGY AND 5TH INTERNATIONAL CONGRESS ON VIRTUAL MICROSCOPY, 8 June 2012 (2012-06-08), XP055404537
Attorney, Agent or Firm:
KOYO INTERNATIONAL PATENT FIRM (JP)
Patent business corporation Mitsuaki international patent firm (JP)
Patent business corporation Mitsuaki international patent firm (JP)
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