Title:
BIOLUMINESCENCE ASSAY KIT OF HUMAN CARBOXYLESTERASE 1, METHOD FOR USE AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/049423
Kind Code:
A1
Abstract:
Provided are a bioluminescence assay kit of human carboxylesterase 1, a method for use and an application thereof. The kit comprises an A liquid, a B liquid, a C liquid, and a quality control standard. The A liquid is a 100 mM phosphate buffer solution, with pH = 5.5-10, and preferably a buffer system with pH of 6.5. The B liquid is a 0.1-10 mM solution which contains a human carboxylesterase 1 specific biological probe substrate, wherein a solvent is dimethyl sulfoxide. The C liquid is a luciferin detection reagent. The quality control standard is a phosphate solution with 5 mg/ml of hCE1 and pH of 6.5. The human carboxylesterase 1 specific biological probe substrate is 4,5-dihydro-2(6-hydroxy-2-benzimidazole)4-carboxylic acid thiazole methyl ester or 4,5-dihydro-2(6-amino-2-benzimidazole)4-carboxylic acid thiazole methyl ester. The kit can be used for detection of existence of hCE1 in various biological samples and quantitative assay of enzyme activity thereof, and can also be used for quickly selecting an inhibitor/inducer for the hCE1.
Inventors:
YANG LING (CN)
WANG DANDAN (CN)
GE GUANGBO (CN)
LV XIA (CN)
YU YANG (CN)
DU XUNFU (CN)
WANG DANDAN (CN)
GE GUANGBO (CN)
LV XIA (CN)
YU YANG (CN)
DU XUNFU (CN)
Application Number:
PCT/CN2015/000821
Publication Date:
March 30, 2017
Filing Date:
November 26, 2015
Export Citation:
Assignee:
DALIAN INST CHEMICAL PHYSICS CAS (CN)
International Classes:
G01N21/76; G01N33/68
Domestic Patent References:
| WO2015014092A1 | 2015-02-05 |
Other References:
WU, CHUNLING ET AL.: "Preparation, Characterization and Application of Polyclonal Antibody Against Human Carboxylesterases-II", CHINESE JOURNAL OF CELLULAR AND MOLECULAR IMMUNOLOGY, vol. 25, no. 2, 28 February 2009 (2009-02-28)
Attorney, Agent or Firm:
SHENYANG CHENCHUANG TECHNICAL PATENT AGENT LTD. (CN)
Download PDF: