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Patent Searching and Data


Title:
BIOMAGNETIC MICROSPHERE AND PREPARATION METHOD AND USE METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2020/253834
Kind Code:
A1
Abstract:
A biomagnetic microsphere and a preparation method and a method for protein isolation and purification therefor. The outer surface of a magnetic microsphere body of the biomagnetic microsphere has at least one linear polymer with a branched chain; one end of the linear polymer with a branched chain is covalently coupled to the outer surface of the magnetic microsphere body, and other parts are free on the outer surface of the magnetic microsphere body; a backbone of the linear polymer is a polyolefin backbone, and no cross-linking agent is required in the backbone forming process of the linear polymer. The prepared biomagnetic microsphere can implement efficient elution of target proteins and effectively reduce the retention time and retention ratio of the target proteins, and it is easy to operate and widely used.

Inventors:
GUO MIN (CN)
WU LIANG (CN)
XU LIQIONG (CN)
ZHANG ZHENG (CN)
SU JIE (CN)
YU XUE (CN)
Application Number:
PCT/CN2020/097155
Publication Date:
December 24, 2020
Filing Date:
June 19, 2020
Export Citation:
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Assignee:
KANGMA HEATHCODE SHANGHAI BIOTECH CO LTD (CN)
International Classes:
H01F1/01; B01J20/22; C08F220/04; C08F220/10
Foreign References:
CN104402003A2015-03-11
CN104402003A2015-03-11
CN103406100A2013-11-27
US20130149772A12013-06-13
CN201910540132A2019-06-21
CN108690139A2018-10-23
CN109423496A2019-03-05
CN106978439A2017-07-25
CN110408635A2019-11-05
CN110551700A2019-12-10
CN110093284A2019-08-06
CN110845622A2020-02-28
CN110938649A2020-03-31
CN201811619819A2018-12-28
Other References:
SAMBROOK: "Molecular Cloning: A Laboratory Manual", 1989, COLD SPRING HARBOR LABORATORY PRESS
"A Laboratory Manual for Cell-Free Protein Synthesis", 2008, article "Cell-free protein synthesis: methods and protocols[M"
MOLECULAR AND CELLULAR BIOLOGY, vol. 10, no. 1, 1990, pages 353 - 360
See also references of EP 3989243A4
Attorney, Agent or Firm:
SHANGHAI BESHINING LAW OFFICE (CN)
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