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Title:
BIOMATERIAL QUANTITY MEASUREMENT METHOD, IMAGE PROCESSING DEVICE, PATHOLOGY DIAGNOSIS SUPPORT SYSTEM, AND PROGRAM
Document Type and Number:
WIPO Patent Application WO/2019/078230
Kind Code:
A1
Abstract:
The present invention addresses the problem of making it possible to analyze the quantity of a biomaterial without being affected by either of variation in the brightness per particle of fluorescent dye accumulation particles and variation in the number of fluorescent dye accumulation particles adhering to a target specimen. Provided is a biomaterial quantity measurement method for measuring the expression level of a specific biomaterial in a target specimen that has been dyed using fluorescent dye accumulation particles, wherein the method comprises: an input step for inputting a first fluorescence image obtained by imaging the target specimen; a brightness calculation step for extracting a bright-spot part from the first fluorescence image and calculating a first brightness value that is the brightness value of the bright-spot part; and a particle number calculation step for calculating the number of fluorescent dye accumulation particles included in the extracted bright-spot part in the first fluorescent image using the distribution of the first brightness value, a second brightness value that is the brightness value of an extracted bright-spot part in a second fluorescence image obtained by imaging a reference specimen for which the expression level of the specific biomaterial has been measured in advance, and a third brightness value that is the brightness value of each light-spot part that represents emission of light from the fluorescent dye accumulation particles in a third fluorescence image obtained by imaging a prepared slide on which the fluorescent dye accumulation particles were scattered without agglomerating.

Inventors:
OZAKI YUICHI (JP)
Application Number:
JP2018/038581
Publication Date:
April 25, 2019
Filing Date:
October 17, 2018
Export Citation:
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Assignee:
KONICA MINOLTA INC (JP)
International Classes:
G01N33/483; G01N21/64; G01N33/48; G01N33/53
Domestic Patent References:
WO2016006096A12016-01-14
WO2016129061A12016-08-18
WO2016125236A12016-08-11
WO2012029342A12012-03-08
WO2016129061A12016-08-18
Foreign References:
JP2009098151A2009-05-07
JP2006177956A2006-07-06
US20130338014A12013-12-19
JP2004157018A2004-06-03
JP5593221B22014-09-17
Attorney, Agent or Firm:
KOYO INTERNATIONAL PATENT FIRM (JP)
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