Title:
BIOMATERIAL QUANTITY MEASUREMENT METHOD, IMAGE PROCESSING DEVICE, PATHOLOGY DIAGNOSIS SUPPORT SYSTEM, AND PROGRAM
Document Type and Number:
WIPO Patent Application WO/2019/078230
Kind Code:
A1
Abstract:
The present invention addresses the problem of making it possible to analyze the quantity of a biomaterial without being affected by either of variation in the brightness per particle of fluorescent dye accumulation particles and variation in the number of fluorescent dye accumulation particles adhering to a target specimen. Provided is a biomaterial quantity measurement method for measuring the expression level of a specific biomaterial in a target specimen that has been dyed using fluorescent dye accumulation particles, wherein the method comprises: an input step for inputting a first fluorescence image obtained by imaging the target specimen; a brightness calculation step for extracting a bright-spot part from the first fluorescence image and calculating a first brightness value that is the brightness value of the bright-spot part; and a particle number calculation step for calculating the number of fluorescent dye accumulation particles included in the extracted bright-spot part in the first fluorescent image using the distribution of the first brightness value, a second brightness value that is the brightness value of an extracted bright-spot part in a second fluorescence image obtained by imaging a reference specimen for which the expression level of the specific biomaterial has been measured in advance, and a third brightness value that is the brightness value of each light-spot part that represents emission of light from the fluorescent dye accumulation particles in a third fluorescence image obtained by imaging a prepared slide on which the fluorescent dye accumulation particles were scattered without agglomerating.
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Inventors:
OZAKI YUICHI (JP)
Application Number:
PCT/JP2018/038581
Publication Date:
April 25, 2019
Filing Date:
October 17, 2018
Export Citation:
Assignee:
KONICA MINOLTA INC (JP)
International Classes:
G01N33/483; G01N21/64; G01N33/48; G01N33/53
Domestic Patent References:
| WO2016006096A1 | 2016-01-14 | |||
| WO2016129061A1 | 2016-08-18 | |||
| WO2016125236A1 | 2016-08-11 | |||
| WO2012029342A1 | 2012-03-08 | |||
| WO2016129061A1 | 2016-08-18 |
Foreign References:
| JP2009098151A | 2009-05-07 | |||
| JP2006177956A | 2006-07-06 | |||
| US20130338014A1 | 2013-12-19 | |||
| JP2004157018A | 2004-06-03 | |||
| JP5593221B2 | 2014-09-17 |
Other References:
See also references of EP 3699588A4
Attorney, Agent or Firm:
KOYO INTERNATIONAL PATENT FIRM (JP)
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