Title:
BIOMIMETIC SUPPORT FOR THREE-DIMENSIONAL CELL CULTURING, METHOD FOR MANUFACTURING SAME, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2016/010377
Kind Code:
A1
Abstract:
The present invention provides a method for manufacturing a composite nanofiber support and a fish collagen/synthetic polymer nanofiber support manufactured by the method, wherein the method comprises: a first step for dissolving a synthetic polymer in an organic solvent; a second step for dissolving a fish collagen in water to prepare an aqueous fish collagen solution; a third step for adding the aqueous fish collagen solution prepared in the second step to the synthetic polymer solution prepared in the first step, followed by mixing; and a fourth step for electrospinning the mixture liquid prepared in the third step to manufacture a nanofiber support. In addition, the present invention provides a method for three-dimensional cell culturing using an agarose-collagen-alginate composite hydrogel support, and more specifically, to a method for three-dimensional cell culturing, the method comprising the steps of: mixing an alginate solution and cells, followed by culturing; adding a collagen solution to the cultured mixture to prevent the detachment of the cells; and adding an agarose solution to the collagen-added mixture, followed by standing and gelation.
Inventors:
YOON SIK (KR)
KWAK JONG-YOUNG (KR)
JIN SONG-WAN (KR)
JEONG YOUNG HUN (KR)
KIM CHANG GUN (KR)
CHOI DA JEONG (KR)
KANG HAE YEONG (KR)
FAZLI SUBHAN (KR)
IKRAM MUHAMAD (KR)
KWAK JONG-YOUNG (KR)
JIN SONG-WAN (KR)
JEONG YOUNG HUN (KR)
KIM CHANG GUN (KR)
CHOI DA JEONG (KR)
KANG HAE YEONG (KR)
FAZLI SUBHAN (KR)
IKRAM MUHAMAD (KR)
Application Number:
PCT/KR2015/007394
Publication Date:
January 21, 2016
Filing Date:
July 16, 2015
Export Citation:
Assignee:
PUSAN NAT UNIV IND COOP FOUND (KR)
International Classes:
C12M3/00; C12N5/07; C12N5/09; C12Q1/02; D04H1/728; G01N33/50
Foreign References:
| KR20070024092A | 2007-03-02 | |||
| JP2004033135A | 2004-02-05 | |||
| KR20100051294A | 2010-05-17 |
Other References:
ZHOU, G. ET AL.: "Research on the Structure of Fish Collagen Nanofibers Influenced Cell Growth", JOURNAL OF NANOMATERIALS
Attorney, Agent or Firm:
TAEBAEK INTELLECTUAL PROPERTY LAW FIRM (KR)
특허법인 태백 (KR)
특허법인 태백 (KR)
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