Title:
BIOMOLECULE DETECTION METHOD
Document Type and Number:
WIPO Patent Application WO/2019/069893
Kind Code:
A1
Abstract:
This biomolecule detection method is for detecting biomolecules included in a sample and comprises: a separation step (S1) in which the biomolecules in a sample are separated by thin-layer chromatography; an irradiation step (S2) in which the biomolecules are irradiated with excitation light from a light source (2); and a detection step (S3) in which fluorescence generated as a result of the excitation light is detected by a light-receiving element (3). The fluorescence is autofluorescence from biomolecules.
Inventors:
TANAKA HIROYUKI
SHINOHARA JUNPEI
SHINOHARA JUNPEI
Application Number:
PCT/JP2018/036808
Publication Date:
April 11, 2019
Filing Date:
October 02, 2018
Export Citation:
Assignee:
PANASONIC IP MAN CO LTD (JP)
International Classes:
G01N30/95; G01N27/447; G01N33/68
Domestic Patent References:
| WO2016096078A1 | 2016-06-23 |
Foreign References:
| JP2005534914A | 2005-11-17 | |||
| JP2012233774A | 2012-11-29 | |||
| JP2008241447A | 2008-10-09 |
Other References:
LINARES APONTE, R. ET AL.: "SIMPLE THIN LAYER CHROMATOGRAPHY METHOD WITH FIBRE OPTIC REMOTE SENSOR FOR FLUORIMETRIC QUANTIFICATION OF TRYPTOPHAN AND RELATED METABOLITES", J LIQ CHROMATOGR RELAT TECHNOL, vol. 19, no. 5, 1996, pages 687 - 698
DILLON, J. ET AL.: "THE PHOTOCHEMISTRY OF SPECIFIC TRYPTOPHAN RESIDUES IN PROTEINS AS ANALYZED BY THE FLUORESCENT SCANNING OF TRYPTIC PEPTIDE MAPS", PHOTOCHEMISTRY AND PHOTOBIOLOGY, vol. 45, no. 1, 1987, pages 147 - 150, XP055591787
BHUSHAN, R. ET AL.: "Thin Layer Chromatography of Peptides and Proteins: A Review", BIOMEDICAL CHROMATOGRAPHY, vol. 3, no. 3, 1989, pages 95 - 104, XP055591790
MUNIER, R. L. ET AL.: "Separation of Amino Acids. Possibilities Offered by Optimized Associations of Low-Voltage Electrophoresis and Chromatography on Finely Textured Paper and on Cellulose Thin-Layers", CHROMATOGRAPHIA, vol. 11, no. 9, 1978, pages 508 - 512, XP055591791
DILLON, J. ET AL.: "THE PHOTOCHEMISTRY OF SPECIFIC TRYPTOPHAN RESIDUES IN PROTEINS AS ANALYZED BY THE FLUORESCENT SCANNING OF TRYPTIC PEPTIDE MAPS", PHOTOCHEMISTRY AND PHOTOBIOLOGY, vol. 45, no. 1, 1987, pages 147 - 150, XP055591787
BHUSHAN, R. ET AL.: "Thin Layer Chromatography of Peptides and Proteins: A Review", BIOMEDICAL CHROMATOGRAPHY, vol. 3, no. 3, 1989, pages 95 - 104, XP055591790
MUNIER, R. L. ET AL.: "Separation of Amino Acids. Possibilities Offered by Optimized Associations of Low-Voltage Electrophoresis and Chromatography on Finely Textured Paper and on Cellulose Thin-Layers", CHROMATOGRAPHIA, vol. 11, no. 9, 1978, pages 508 - 512, XP055591791
Attorney, Agent or Firm:
NII, Hiromori et al. (JP)
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