Title:
BIOPOLYMER ANALYSIS METHOD, BIOPOLYMER ANALYZER, AND BIOPOLYMER ANALYSIS CHIP
Document Type and Number:
WIPO Patent Application WO/2011/121881
Kind Code:
A1
Abstract:
When sequence analysis is carried out using multiple nanopores, trapping samples in nanopores is not necessarily 100% efficient and time is wasted in measuring nanopores in which samples are not trapped, resulting in low measurement efficiency. In the disclosed biopolymer analysis method, a marker substance is bonded to samples, and the samples to which the marker substance has been bonded are trapped in nanopores. A device for observing the marker substance is used in order to observe the marker substance, thus allowing confirmation as to whether or not the samples have been trapped in individual nanopores. By taking measurements of only the nanopores in which the samples have been trapped, measurement efficiency is increased.
Inventors:
AKAHORI RENA (JP)
ANAZAWA TAKASHI (JP)
OZAWA SATOSHI (JP)
YAZAWA YOSHIAKI (JP)
SAKAI TOMOYUKI (JP)
NODA HIDEYUKI (JP)
ANAZAWA TAKASHI (JP)
OZAWA SATOSHI (JP)
YAZAWA YOSHIAKI (JP)
SAKAI TOMOYUKI (JP)
NODA HIDEYUKI (JP)
Application Number:
PCT/JP2011/000997
Publication Date:
October 06, 2011
Filing Date:
February 23, 2011
Export Citation:
Assignee:
HITACHI HIGH TECH CORP (JP)
AKAHORI RENA (JP)
ANAZAWA TAKASHI (JP)
OZAWA SATOSHI (JP)
YAZAWA YOSHIAKI (JP)
SAKAI TOMOYUKI (JP)
NODA HIDEYUKI (JP)
AKAHORI RENA (JP)
ANAZAWA TAKASHI (JP)
OZAWA SATOSHI (JP)
YAZAWA YOSHIAKI (JP)
SAKAI TOMOYUKI (JP)
NODA HIDEYUKI (JP)
International Classes:
C12N15/09; C12M1/00; C12Q1/68; G01N21/64; G01N27/00; G01N27/22; G01N33/53; G01N37/00; G01N27/416
Foreign References:
| JP2007170957A | 2007-07-05 | |||
| US20060057585A1 | 2006-03-16 | |||
| US20040144658A1 | 2004-07-29 |
Other References:
TROPINI, C. ET AL.: "Multi-nanopore force spectroscopy for DNA analysis.", BIOPHYS. J., vol. 92, no. 5, March 2007 (2007-03-01), pages 1632 - 1637
PENG, H. ET AL.: "Reverse DNA translocation through a solid-state nanopore by magnetic tweezers.", NANOTECHNOLOGY, vol. 20, no. 18, 6 May 2009 (2009-05-06), pages 185101-1 - 185101-8
BRANTON, D. ET AL., NAT. BIOTECH., vol. 26, 2008, pages 1146 - 1153
KEYSER, U. F. ET AL., NAT. PHYS., vol. 2, 2006, pages 473
TROPINI, C. ET AL., BIOPHYSICAL J., vol. 92, 2007, pages 1632 - 1637
PENG, H. ET AL.: "Reverse DNA translocation through a solid-state nanopore by magnetic tweezers.", NANOTECHNOLOGY, vol. 20, no. 18, 6 May 2009 (2009-05-06), pages 185101-1 - 185101-8
BRANTON, D. ET AL., NAT. BIOTECH., vol. 26, 2008, pages 1146 - 1153
KEYSER, U. F. ET AL., NAT. PHYS., vol. 2, 2006, pages 473
TROPINI, C. ET AL., BIOPHYSICAL J., vol. 92, 2007, pages 1632 - 1637
Attorney, Agent or Firm:
INOUE, Manabu et al. (JP)
Manabu Inoue (JP)
Manabu Inoue (JP)
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Claims: