Title:
BIOREACTORS WITH SUBSTANCE INJECTION CAPACITY
Document Type and Number:
WIPO Patent Application WO2004020341
Kind Code:
A3
Abstract:
A bioreactor with substance injection capability. In one embodiment, the bioreactor includes a first substrate (1124) having a first surface (1124a), an opposite second surface (1124b) and edges. The bioreactor further includes a second substrate (1121) having a first surface (1121a) and an opposite second surface (1121b), defining a cavity (1121c) with a bottom surface (1121d), where the bottom surface (1121d) is located therebetween the first surface (1121a) and the second surface (1121b). The first surface (1124a) of the first substrate (1124) is received by the second surface (1121b) of the second substrate (1121) to cover the cavity (1121c) so as to form a chamber for receiving cells and a liquid medium. A port (1122) is formed in the second substrate (1121) between the bottom surface (1121d) and the first surface (1124a) of the second substrate (1121). As formed, the port (1122) is in fluid communication with the chamber to allow a stream of substances to be introduced into the chamber. The stream of substances is controlled so as to provide a gradient, or a concentration gradient of the substance, to the chamber. The stream of substance includes a substance affecting the growth of cells such as chemokine.
Inventors:
WIKSWO JOHN P (US)
BAUDENBACHER FRANZ J (US)
HASELTON FREDERICK R (US)
HOFMEISTER WILLIAM H (US)
LIN CHARLES P (US)
MCCAWLEY LISA J (US)
STREMLER MARK (US)
WEAVER ALISSA (US)
BAUDENBACHER FRANZ J (US)
HASELTON FREDERICK R (US)
HOFMEISTER WILLIAM H (US)
LIN CHARLES P (US)
MCCAWLEY LISA J (US)
STREMLER MARK (US)
WEAVER ALISSA (US)
Application Number:
PCT/US2003/026800
Publication Date:
April 29, 2004
Filing Date:
August 27, 2003
Export Citation:
Assignee:
UNIV VANDERBILT (US)
WIKSWO JOHN P (US)
BAUDENBACHER FRANZ J (US)
HASELTON FREDERICK R (US)
HOFMEISTER WILLIAM H (US)
LIN CHARLES P (US)
MCCAWLEY LISA J (US)
STREMLER MARK (US)
WEAVER ALISSA (US)
WIKSWO JOHN P (US)
BAUDENBACHER FRANZ J (US)
HASELTON FREDERICK R (US)
HOFMEISTER WILLIAM H (US)
LIN CHARLES P (US)
MCCAWLEY LISA J (US)
STREMLER MARK (US)
WEAVER ALISSA (US)
International Classes:
B01L3/00; C12M3/04; (IPC1-7): B01D57/02; G01N27/00; C12M1/12; C12M1/34
Foreign References:
| US6124138A | 2000-09-26 | |||
| US6267858B1 | 2001-07-31 |
Other References:
See also references of EP 1545750A4
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