Title:
BIOSENSORS WITH A DIRECT ELECTRICAL OUTPUT
Document Type and Number:
WIPO Patent Application WO/2016/089769
Kind Code:
A3
Abstract:
A biosensor that employs a bacterium in a biofilm present on one of two electrodes of an electrochemical cell. The bacterium is genetically modified by having deleted at least one native gene that encodes for the enzymatic transformation of a molecular moiety, and having substituted for the deleted native gene a different gene having a transcription factor that is under the control of an inducible promoter in conjunction with an inducer molecule. When the molecular moiety is present in a specimen of interest that is in contact with the biofilm, an electric current is generated in response to the presence of the inducer molecule. In the absence of the inducer molecule, no electricity is generated. The electric signal that is generated can be analyzed to determine the presence and the quantity of the inducer molecule in the specimen of interest.
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Inventors:
LOVLEY DEREK R (US)
UEKI TOSHIIYUKI (US)
NEVIN KELLY P (US)
UEKI TOSHIIYUKI (US)
NEVIN KELLY P (US)
Application Number:
PCT/US2015/062994
Publication Date:
August 11, 2016
Filing Date:
November 30, 2015
Export Citation:
Assignee:
UNIV MASSACHUSETTS (US)
International Classes:
H01M8/16; C12N1/11; C12N1/20; C12N15/52
Foreign References:
| US20140004578A1 | 2014-01-02 |
Other References:
POLITI, N ET AL.: "Half-Life Measurements of Chemical Inducers for Recombinant Gene Expression.", J BIOL ENG., vol. 8, no. 1, 1 February 2014 (2014-02-01), pages 1 - 10
FERNANDEZ, A ET AL.: "Development and Validation of a Liquid Chromatography-Mass Spectrometry assay for the quantitation of IPTG in E. Coli fed-batch cultures.", ANAL CHEM., vol. 82, no. 13, 1 July 2010 (2010-07-01), pages 5728 - 5734
CORD-RUWISCH, RC ET AL.: "Growth of Geobacter sulfurreducens with Acetate in Syntrophic Cooperation with Hydrogen-Oxidizing Anaerobic Partners.", APPL ENVIRON MICROBIOL., vol. 64, no. 6, June 1998 (1998-06-01), pages 2232 - 2236
FERNANDEZ, A ET AL.: "Development and Validation of a Liquid Chromatography-Mass Spectrometry assay for the quantitation of IPTG in E. Coli fed-batch cultures.", ANAL CHEM., vol. 82, no. 13, 1 July 2010 (2010-07-01), pages 5728 - 5734
CORD-RUWISCH, RC ET AL.: "Growth of Geobacter sulfurreducens with Acetate in Syntrophic Cooperation with Hydrogen-Oxidizing Anaerobic Partners.", APPL ENVIRON MICROBIOL., vol. 64, no. 6, June 1998 (1998-06-01), pages 2232 - 2236
Attorney, Agent or Firm:
MILSTEIN, Joseph, B. (2000 Commonwealth Avenue Suite 40, Newton MA, US)
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