BIOTHERAPEUTIC ENTEROCOCCUS ISOLATES

FIELD OF THE INVENTION

The present invention relates to new strains of Enterococcus sp. useful for bacteriotherapy.

BACKGROUND OF THE INVENTION

The human intestinal microbiota consists of trillions of microorganisms including at least 100 prevalent and at least 1000 less common bacterial species, harbouring over 100-fold more genes than those present in the human genome. The intestinal microbiota is composed predominantly of bacteria, yet also contains archaea, protozoa, and viruses. The microbiota performs vital functions essential to health maintenance, including food processing, digestion of complex indigestible polysaccharides and synthesis of vitamins, and it secretes bioactive metabolites with diverse functions, ranging from inhibition of pathogens, metabolism of toxic compounds to modulation of host metabolism.

Inflammatory bowel disease (IBD) is an increasingly prevalent, currently incurable condition believed to be caused by an abnormal immune response to the resident gut microbiome in genetically susceptible patients (Graham and Xavier, 2020). Affecting both adults and children, adult cohorts are frequently confounded by co-morbidities, disease course, existing medications and lifestyle factors typically absent in newly diagnosed paediatric populations. High-throughput sequencing of both adult and paediatric patient cohorts has now provided detailed taxonomic understanding of microbiome composition in IBD (Schirmer et al., 2019). However, existing studies are dominated by faecal sampling from adult cohorts, with phenotypic investigation of human bacteria largely limited to genomic predictions and correlative associations (reviewed by Ni et al. 2017). The variation in disease state across the gastrointestinal tract, coupled with confounding factors in adult patient cohorts, necessitates detailed host and microbiome investigation of mucosal samples in paediatric patient cohorts.

Studies have shown that bacteriotherapy using beneficial bacterial isolates can be used to treat and/or prevent diseases/disorders such as irritable bowel syndrome, inflammatory bowel diseases, ulcers, or stomach cancer. Thus, there is the need for the identification of further bacteria for use in bacteriotherapy. SUMMARY OF THE INVENTION

The present inventors have isolated a new Enterococcus sp. useful for bacteriotherapy.

The present inventors have identified non-inflammatory strains of Enterococcus sp. Thus, in an aspect the present invention provides a method of reducing or preventing gastrointestinal tract mucosal inflammation in a subject, the method comprising administering to the subject a therapeutically effective amount of a biotherapeutic composition comprising a non-inflammatory strain of Enterococcus sp.

In an embodiment, the non-inflammatory strain comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 44, or a nucleotide sequence at least 90% identical to one or more of SEQ ID NO's 1 to 44. Examples of such strains include, but are not limited to; i) Enterococcus sp. CC00149 deposited under V19/018754 on 9 September 2019 at the National Measurement Institute, Australia, ii) Enterococcus sp. CC00259 deposited under V19/018755 on 9 September 2019 at the National Measurement Institute, Australia, and iii) Enterococcus sp. CC00620 deposited under V21/013048 on 29 June 2021 at the National Measurement Institute, Australia.

In a further aspect, the present invention provides a method of treating or preventing a dysbiosis of the gastrointestinal tract in a subject, the method comprising administering to the subject a therapeutically effective amount of a biotherapeutic composition comprising a strain of Enterococcus sp. which comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 74, or a nucleotide sequence at least 90% identical to one or more of SEQ ID NO's 1 to 74.

In an embodiment, the Enterococcus sp. is a non-inflammatory strain and comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 44, or a nucleotide sequence at least 90% identical to one or more of SEQ ID NO's 1 to 44.

In an alternate embodiment, the Enterococcus sp, is an inflammatory strain and comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 90% identical to one or more of SEQ ID NO's 45 to 74. Examples of such strains include, but are not limited to; i) Enterococcus sp. CC00064 deposited under V21/013046 on 29 June 2021 at the National Measurement Institute, Australia, ii) Enterococcus sp. CC00619 deposited under V21/013047 on 29 June 2021 at the National Measurement Institute, Australia, iii) Enterococcus sp. CC00262 deposited under V20/006238 on 18 March 2020 at the National Measurement Institute, Australia, and iv) Enterococcus sp. CC0002 deposited under V21/014119 on 20 July 2021 at the National Measurement Institute, Australia.

In an embodiment, the dysbiosis and/or inflammation is associated with one or more of inflammatory bowel disease (IBD), pouchitis, irritable bowel syndrome (IBS), an enteric bacterial infection, a metabolic disease, a neuropsychiatric disorder, an autoimmune disease, an allergic disorder, hepatic encephalopathy, or a cancer. In an embodiment, the IBD is ulcerative colitis (UC) or Crohn's disease.

In an embodiment, the composition further comprises a prebiotic.

In an embodiment, the composition further comprises a carrier.

In an embodiment, the composition further comprises insoluble fiber, a buffer, an osmotic agent, an antifoaming agent, and/or a preservative.

In an embodiment, wherein the composition comprises a chemostat medium.

In an embodiment, the composition is a saline composition.

In an embodiment, the composition is administered orally or rectally.

In an embodiment, the composition further comprises a stabiliser and/or a cryoprotectant.

In an embodiment, the composition is freeze dried.

In an embodiment, the composition is the form of a capsule, a tablet, or an enema. In an embodiment, the capsule or tablet is enteric-coated, pH dependant, slow-release, and/or gastro-resistant.

In an embodiment, the strain is present in the composition at about 10 ³ to about 10 ¹³ , or about 10 ⁴ to about 10 ¹² , or about 10 ⁵ to about 10 ¹¹ , or about 10 ⁶ to about 10 ¹⁰ , or about 10 ⁷ to about 10 ⁹ , cfu per gram.

In an embodiment, the subject is a human.

In a further aspect, the present invention provides an isolated non-inflammatory strain of Enterococcus sp. In an embodiment, the non-inflammatory strain comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 44, or a nucleotide sequence at least 90% identical to one or more of SEQ ID NO's 1 to 44.

In another aspect, the present invention provides an isolated strain of Enterococcus sp. which comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 74, or a nucleotide sequence at least 90% identical to one or more of SEQ ID NO's 1 to 74. In an embodiment, the Enterococcus sp, strain is an inflammatory strain and comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 90% identical to one or more of SEQ ID NO's 45 to 74.

In a further aspect, the present invention provides a composition comprising at least one strain of the invention. In an embodiment, the composition is a biotherapeutic composition.

In an aspect, the present invention provides a method of preparing a biotherapeutic composition, the method comprising: i) culturing at least one strain of Enterococcus sp. of the invention, and ii) mixing the Enterococcus sp. obtained in i) with a pharmaceutically acceptable carrier.

In a further aspect, the present invention provides a method of analysing the microbiome of the gastrointestinal tract of a subject, the method comprising; i) obtaining a sample comprising bacteria from the gastrointestinal tract of the subject, ii) analysing the sample for the presence of a strain of Enterococcus sp. of the invention.

In an embodiment, DNA of, or extracted from, the sample is analysed.

In an embodiment, the sample is analysed by DNA amplification and/or DNA hybridization.

In an embodiment, the presence of a strain of Enterococcus sp. which comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 90% identical to one or more of SEQ ID NO's 45 to 74, suggests that the gastrointestinal tract is inflamed, or that a previously detected dysbiosis and/or gastrointestinal tract inflammation is at least partly due to the strain.

In an embodiment, the presence of a strain of Enterococcus sp. which comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 44, or a nucleotide sequence at least 90% identical to one or more of SEQ ID NO's 1 to 44, suggests that the gastrointestinal tract is not inflamed, and/or that the gastrointestinal tract comprises non-inflammatory strains of Enterococcus sp.

In an embodiment, a non-inflammatory strain of the invention is, or can be, detected using the primer pairs; i) CGAGGTACACCGTAAGTTGTTGCT (SEQ ID NO: 75) and

ACCAGGACACGTAGACTTCACAGT (SEQ ID NO: 76), or ii) TGGCCCAAAGATACGTGTTCCAC (SEQ ID NO: 77) and

CGAACAAAATCAGGAGCTCGTCGT (SEQ ID NO: 78).

In an embodiment, an inflammatory strain of the invention is, or can be, detected using the primer pairs; i) ACTGCTGAGTGCCCACGCTT (SEQ ID NO: 79) and GAGGCACTTGCTAGCGTCACAC (SEQ ID NO: 80), ii) TGCGTGTAGGCATCATCCGTGA (SEQ ID NO: 81) and

GACACAGCGGCATCAGCAAGT (SEQ ID NO: 82), iii) CCTTTACCATGGCCGTGTAGC (SEQ ID NO: 83) and

CCATGAATGGTGTTCCTCCTTCTC (SEQ ID NO: 84), or iv) AAACTTGCTGATGCCGCTGT (SEQ ID NO: 85) and

CCGCCTTTACCGATAACCATACC (SEQ ID NO: 86).

Also provided is the use of at least one strain of Enterococcus sp. of the invention for manufacture of a medicament for treating or preventing a dysbiosis of the gastrointestinal tract.

Also provided is the use of at least one non-inflammatory strain of Enterococcus sp for manufacture of a medicament for reducing or preventing gastrointestinal tract mucosal inflammation in a subject.

Also provided is at least one strain of Enterococcus sp. of the invention for use in treating or preventing a dysbiosis of the gastrointestinal tract.

Any embodiment herein shall be taken to apply mutatis mutandis to any other embodiment unless specifically stated otherwise.

The present invention is not to be limited in scope by the specific embodiments described herein, which are intended for the purpose of exemplification only. Functionally-equivalent products, compositions and methods are clearly within the scope of the invention, as described herein.

Throughout this specification, unless specifically stated otherwise or the context requires otherwise, reference to a single step, composition of matter, group of steps or group of compositions of matter shall be taken to encompass one and a plurality (i.e. one or more) of those steps, compositions of matter, groups of steps or group of compositions of matter.

The invention is hereinafter described by way of the following non-limiting Examples and with reference to the accompanying figures. BRIEF DESCRIPTION OF THE ACCOMPANYING DRAWINGS

Figure 1. Workflow for direct microbial culturing and sequencing from patient derived intestinal biopsy samples. Workflow for the culturing of bacterial communities directly from mucosal samples, to minimise the amount of eukaryotic DNA present.

Figure 2. A subset of IBD samples demonstrate clade specific bacterial enrichment. Differences in relative proportion of key Enterococcus clades in the control and IBD patients (P <0.05; KS) highlights the existence of a distinct subset of IBD patients.

Figure 3. Fewer virulence genes are associated with the isolates achieving high cell cytotoxicity in stimulated Caco2 cells. The number of virulence genes in the isolates of interest from each clade was assessed, using a percent identity cut-off of> 70% (P < 0.01 ; Mann-Whitney U test).

Figure 4. In vitro phenotypic validation of candidate bacteria demonstrates a clade specific difference in cell cytotoxicity. Cell cytotoxicity at 24 hours by LDH assay in Caco2 intestinal epithelial cells stimulated with one of twelve isolates representing the IBD clade (six isolates on the right) and control clade at a multiplicity of infection (MOI) of 10:1 (n = 3 biological replicates, mean ± SEM). Bacterial clades are represented by the phylogenetic tree below the graph, depicting the relationship between isolates. Statistics represent a comparison between the clade associated with IBD vs control clade (P = 0.0006; Ratio paired t-test). SEM images of each isolate also allow for morphological visualisation of each candidate bacteria.

Figure 5. Scanning electron microscopy (SEM) of the 12 isolates used to stimulate Caco2 cells, a. The six isolates in the control-associated clade, that cause greater cell cytotoxicity when stimulating Caco2 cells, b, The six isolates in the IBD-enriched clade that cause less cell cytotoxic activity when stimulating Caco2 cells.

Figure 6. Viability of bacterial isolates is essential to achieve the cytotoxic phenotype. A representative isolate from each of the clades under investigation was selected (CC00064 and CC00149) and used to stimulate Caco2 cells, following heat inactivation of the isolates for 60 minutes at 99oC. Concentrations of both 1 mg/ml and 1 pg/ml of heat killed bacteria were used and cell cytotoxicity was assessed at 0, 4, 6, 8 and 24 hours post-stimulation. Ratio paired t-test was used, no significant differences noted, n = 3 biological replicates.

Figure 7. Significantly differentially expressed genes identified following stimulation of Caco2 epithelial cells with CC00064 and CC00149. a, 4 hours post stimulation, b, 8 hours post stimulation, c, 24 hours post stimulation.

Figure 8. In vitro clade specific gene signatures present in inflamed patient derived biopsies, a, Genes for which there was significant differential expression in the in vitro Caco2 experiment between isolates CC00149 and CC00064 at 8 hours were compared to the differential expression results between the inflamed and non-inflamed mucosal samples from each intestinal region. Vertical lines represent genes in the set, which showed upregulation in strain CC00149, compared to CC00064 (weighted by Iog2 fold change). Genes are positioned according to the moderated t-statistic in the clinical group of interest, with highly upregulated genes positioned on the far right, while downregulated genes appear on the far left. Overall gene set enrichment is represented by the enrichment line shown above, b, Heat map showing all genes that were significantly differentially expressed between CC00149 and CC00064 at 8 hours post stimulation, that were also significantly changed in the same direction, between the molecularly inflamed compared to non-inflamed samples, within any of the three clinical sample sites. The top 6 genes were all reduced, and the rest increased with the exception of LMO4 and EPHA2 in the terminal ileum. Significance (P < 0.05) is represented by the asterix (*) in the box. Values displayed are Iog2 fold changes, truncated to ±3.

Figure 9. Cycle threshold (CT) values of quantitative real time PCR for the cytotoxic specific primers (A) and non-cytotoxic specific primers (B) demonstrating clade specificity in both primer sets.

KEY TO THE SEQUENCE LISTING

SEQ ID NO:1 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate CC00149

SEQ ID NO:2 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate CC00259

SEQ ID NO:3 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate CC00261

SEQ ID NO:4 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate CC00260

SEQ ID NO:5 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate CC00620

SEQ ID NO:6 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate CC00152 SEQ ID NO:7 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc056tix_3d_mc056_57_d3

SEQ ID NO:8 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc022rnx_e1

SEQ ID NO:9 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc056tix_3h_mc056_57_h3

SEQ ID NQ:10 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc056tix_3f_mc056_57_f3

SEQ ID NO:11 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc036cna_f03

SEQ ID NO:12 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc056tix_4a_mc056_57_a4

SEQ ID NO:13 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc056tix_3e_mc056_57_e3

SEQ ID NO:14 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc056cix_8d_mc056_57_d8

SEQ ID NO:15 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc056cix_8e_mc056_57_e8

SEQ ID NO:16 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc056tix_3a_mc056_57_a3

SEQ ID NO:17 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc056tix_1 c_mc056_57_c1

SEQ ID NO:18 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc056tix_3b_mc056_57_b3

SEQ ID NO:19 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc036rna2_h03

SEQ ID NQ:20 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc056cix_5f_mc056_57_f5

SEQ ID NO:21 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc056cix_6d_mc056_57_d6

SEQ ID NO:22 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc056tix_3g_mc056_57_g3

SEQ ID NO:23 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc056cix_8c_mc056_57_c8

SEQ ID NO:24 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc056cix 7h mc056 57 h7 SEQ ID NO:25 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc056tix_2b_mc056_57_b2

SEQ ID NO:26 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc017tn_x_b6

SEQ ID NO:27 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc017cn_x_d3

SEQ ID NO:28 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc017cn_x_d2

SEQ ID NO:29 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc026cna2_e06

SEQ ID NQ:30 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc026rna2_g06

SEQ ID NO:31 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc059rn_fx_g10

SEQ ID NO:32 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc017rn_x_f4

SEQ ID NO:33 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc017rn_12f_a

SEQ ID NO:34 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc017tn_12a_ma

SEQ ID NO:35 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc017tn_1a_ma

SEQ ID NO:36 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc017rn_12e_ma

SEQ ID NO:37 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc017cn_12c_ma

SEQ ID NO:38 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc026rnm2_a08

SEQ ID NO:39 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc017tn_x_a1

SEQ ID NQ:40 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc127rifx_b11

SEQ ID NO:41 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc026tnm2_c07

SEQ ID NO:42 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc097cnfx_h6 SEQ ID NO:43 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc127tnfx c2

SEQ ID NO:44 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc127rifx c10

SEQ ID NO:45 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate CC00064

SEQ ID NO:46 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate CC00262

SEQ ID NO:47 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate CC00264

SEQ ID NO:48 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate CC00263

SEQ ID NO:49 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate CC00619

SEQ ID NO:50 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate CC00002 SEQ ID NO:51 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate CC00066 SEQ ID NO:52 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071tn_zx_b3

SEQ ID NO:53 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071cn_fx_e6

SEQ ID NO:54 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071 cn_fx_f8

SEQ ID NO:55 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc001tny_c01

SEQ ID NO:56 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071tn_zx_d11

SEQ ID NO:57 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071tn_zx_d8

SEQ ID NO:58 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc026tna2_b06

SEQ ID NO:59 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071tn_zx_c7

SEQ ID NQ:60 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071cn_fx_h7

SEQ ID NO:61 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071tn_fx_c5

SEQ ID NO:62 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071tn_zx_b4

SEQ ID NO:63 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071tn_fx_d4 SEQ ID NO:64 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071tn_fx_d5

SEQ ID NO:65 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc026cnm1_e07

SEQ ID NO:66 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071tn_fx_c10

SEQ ID NO:67 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071tn_fx_a9

SEQ ID NO:68 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071 tn_fx_a4

SEQ ID NO:69 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071tn_zx_c2

SEQ ID NQ:70 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071cn_zx_g1

SEQ ID NO:71 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071tn_zx_b7

SEQ ID NO:72 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071tn_fx_c1

SEQ ID NO:73 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc026cna1_d06

SEQ ID NO:74 - 16s rRNA nucleotide sequence of Enterococcus sp. isolate mc071tn_fx_b1

SEQ ID NO's 75 to 88 - Oligonucleotide primers

SEQ ID NO's 89 to 217 - Further nucleotide sequences.

Where relevant an n in a sequence indicates the base was not resolved during the sequencing process, and this can be any base.

The sequences are listed below under the heading 'Nucleotide Sequences'.

DETAILED DESCRIPTION OF THE INVENTION

General Techniques and Definitions

Unless specifically defined otherwise, all technical and scientific terms used herein shall be taken to have the same meaning as commonly understood by one of ordinary skill in the art (e.g., in biotherapeutics, prebiotics and the treatment of gastrointestinal tract dysbiosis and mucosal inflammation). The term "and/or ", e.g., "X and/or Y" shall be understood to mean either "X and Y" or "X or Y" and shall be taken to provide explicit support for both meanings or for either meaning.

As used herein, the term about, unless stated to the contrary, refers to +/- 10%, more preferably +/- 5%, of the designated value.

Throughout this specification the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element, integer or step, or group of elements, integers or steps, but not the exclusion of any other element, integer or step, or group of elements, integers or steps.

As used herein, the term "bacteriotherapy" refers to the use of a bacterial isolate to treat or prevent a disease or a condition, or provide a health benefit, in a subject.

As used herein, the term "biotherapeutic" refers to a microorganism, such as bacterial isolate, that is useful for treating or preventing a disease or a condition, or provide a health benefit, in a subject.

The term "biotherapeutic composition", as used herein, refers to a formulation comprising a biotherapeutic preparation formulated together with one or more additional formulary ingredients to obtain a finished formulation suitable for delivery to a subject.

As used herein, the "gastrointestinal tract" refers to the tract from the mouth to the anus which includes all the organs of the digestive system such as the esophagus, stomach, pancreas, liver, gallbladder, small intestine (including the ileum), caecum, large intestine, colon and rectum. Strains of the invention are at least useful for conditions of the terminal ileum, caecum or rectum.

As used herein, a "non-inflammatory strain" refers to a strain of the invention which, when present in the gastrointestinal tract of a subject, preferably a human, is associated with a non-inflamed state. Non-inflammatory strains of the invention have little or no cytotoxicity against mammalian epithelial cells in culture, such as Caco2 cells as described in Example 2. In an embodiment, the strain results in less than 15%, less than 10% or less than 5% of cell death of the mammalian epithelial cells in culture. In an embodiment, a non-inflammatory strain of the invention causes less cell death when exposed to a given cell type than a strain which comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 90% identical to one or more of SEQ ID NO's 45 to 74, such as CC00064, CC00619, CC00262, CC0002 as described herein. In an embodiment, a non-inflammatory strain of the invention is a member of clade 149 as mentioned herein (see, for example, the clade in Figure 4 covering strains CC00620, CC00261 , CC00260, CC00149 and CC00259). Members of this clade have a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 44, or a nucleotide sequence at least 90% identical to one or more of SEQ ID NO's 1 to 44. In an embodiment, following 8 hours incubation with epithelial cells, such as Caco2 cells, one or more or all of the upregulated genes in Table 1 (designated as 1 in the last column) are upregulated by the non-inflammatory strain. In an embodiment, following 24 hours incubation with epithelial cells, such as Caco2 cells, one or more or all of the upregulated genes in Table 2 (designated as 1 in the last column) are upregulated by the non-inflammatory strain. In another embodiment, a non-inflammatory strain of the invention is detectable using primer pairs: CGAGGTACACCGTAAGTTGTTGCT (SEQ ID NO: 75) and ACCAGGACACGTAGACTTCACAGT (SEQ ID NO: 76), and/or TGGCCCAAAGATACGTGTTCCAC (SEQ ID NO: 77) and

CGAACAAAATCAGGAGCTCGTCGT (SEQ ID NO: 78).

As used herein, an "inflammatory strain" refers to a strain of the invention which, when present in the gastrointestinal tract of a subject, preferably a human, is associated with an inflamed state. Inflammatory strains of the invention have cytotoxicity against mammalian epithelial cells in culture, such as Caco2 cells as described in Example 2. In an embodiment, the strain results at least 40%, at least 45% or at least 50% of cell death of the mammalian epithelial cells in culture. In an embodiment, an inflammatory strain of the invention causes more cell death when exposed to a given cell type than a strain which comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 44, or a nucleotide sequence at least 90% identical to one or more of SEQ ID NO's 1 to 44, such as CC00620, CC00261 , CC00260, CC00149 and CC00259 as described herein. In an embodiment, an inflammatory strain of the invention is a member of clade 64 as mentioned herein (see, for example, the clade in Figure 4 covering strains CC00620, CC00261 , CC00260, CC00149 and CC00259). Members of this clade have a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 90% identical to one or more of SEQ ID NO's 45 to 74. In an embodiment, an inflammatory strain of the invention is a member of clade 64 as mentioned herein (see, for example, the clade in Figure 4 covering strains CC00064, CC00619, CC00262, CC00263, and CC00264). Members of this clade have a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 90% identical to one or more of SEQ ID NO's 45 to 74. In an embodiment, following 8 hours incubation with epithelial cells, such as Caco2 cells, one or more or all of the upregulated genes in Table 1 (designated as -1 in the last column) are upregulated by the inflammatory strain. In an embodiment, following 24 hours incubation with epithelial cells, such as Caco2 cells, one or more or all of the upregulated genes in Table 2 (designated as -1 in the last column) are upregulated by the inflammatory strain. In another embodiment, an inflammatory strain of the invention is detectable using primer pairs: ACTGCTGAGTGCCCACGCTT (SEQ ID NO: 79) and GAGGCACTTGCTAGCGTCACAC (SEQ ID NO: 80), TGCGTGTAGGCATCATCCGTGA (SEQ ID NO: 81) and

GACACAGCGGCATCAGCAAGT (SEQ ID NO: 82), CCTTTACCATGGCCGTGTAGC (SEQ ID NO: 83) and CCATGAATGGTGTTCCTCCTTCTC (SEQ ID NO: 84), and/or AAACTTGCTGATGCCGCTGT (SEQ ID NO: 85) and

CCGCCTTTACCGATAACCATACC (SEQ ID NO: 86).

The term "prebiotic", as used herein, means an ingredient for inclusion in a biotherapeutic composition capable of inducing growth or activity of microorganisms in the gastrointestinal system.

As used herein, a "carrier" can be any solvents, diluents, excipients or other vehicles, dispersion or suspension aids, surface active agents, isotonic agents, thickening or emulsifying agents, preservatives, solid binders, lubricants and the like, as suited to the particular dosage form desired.

The term "subject" used within the context of the invention refers to a mammal including humans, livestock such horses, cows, sheep, goats and chickens, dogs and cats. In an embodiment, the subject is a human.

As used herein, the term "pharmaceutically acceptable carrier" component can refer to a component that is not biologically or otherwise undesirable, i.e., the component may be incorporated into a biotherapeutic composition of the invention and administered to a subject as described herein without causing any significant undesirable biological effects or interacting in a deleterious manner with any of the other components of the formulation in which it is contained. The component has generally met the required standards of toxicological and manufacturing.

As used herein, the terms "treat," "treating," "treatment" and grammatical variations thereof mean subjecting an individual subject to a protocol, regimen, process or remedy, in which it is desired to obtain a physiologic response or outcome in that subject. Since every treated subject may not respond to a particular treatment protocol, regimen, process or remedy, treating does not require that the desired physiologic response or outcome be achieved in each and every subject or subject population. Accordingly, a given subject or subject population may fail to respond or respond inadequately to treatment.

As used herein, the term "prevent", "prevented", or "preventing" when used with respect to the treatment of mucosal inflammation in the gastrointestinal refers to a prophylactic treatment which increases the resistance of a subject to mucosal inflammation in the gastrointestinal, in other words, decreases the likelihood that the subject will develop mucosal inflammation in the gastrointestinal as well as a treatment after mucosal inflammation in the gastrointestinal has begun in order to fight the inflammation, e.g., reduce or eliminate it altogether or prevent it from becoming worse.

As used herein, the term "reducing" or variations thereof refer to a reduction but not necessarily a complete abolition of gastrointestinal tract mucosal inflammation in a subject.

As used herein, the term "sample" refers to a collection of biological material obtained from a subject or a subject's surrounding environment, such as soil or water in the area that the subject inhabits. In some embodiments, the sample is obtained directly from the subject. For example, the sample can be a faecal sample or obtained during a colonoscopy. The sample may be in a form taken directly from the subject or surrounding environment, or it may be at least partially purified to remove at least some non-nucleic acid material. The purification may be slight, for instance amounting to no more than the concentration of the solids, or cells, of the sample into a smaller volume or the separation of cells from some or all of the remainder of the sample. In some embodiments, nucleic acids are isolated from the sample. Such isolated preparations include reverse transcription products and/or PCR amplification products of the nucleic acids in the sample. In some embodiments, the predominant nucleic acid is DNA. The nucleic acid preparations can be pure or partially purified nucleic acid preparations. Techniques for the isolation of nucleic acid from samples, including complex samples, are numerous and well known in the art.

The unit "cfu" refers to "colony forming unit", which is the number of bacterial cells as revealed by microbiological counts on agar plates.

Biotherapeutic Strains of Enterococcus sp.

The present invention provides numerous biotherapeutic strains of Enterococcus sp.

In an embodiment, a non-inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 44, or a nucleotide sequence at least 90%, at least 90.5%, at least 91%, at least 91.5%, at least 92%, at least 92.5%, at least 93%, at least 93.5%, at least 94%, at least 94.5%, at least 95%, at least 95.5%, at least 96%, at least 96.5%, at least 97%, at least 97.5%, at least 98%, at least 98.5%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to one or more of SEQ ID NO's 1 to 44.

In an embodiment, a non-inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 44, or a nucleotide sequence at least 94%, at least 94.5%, at least 95%, at least 95.5%, at least 96%, at least 96.5%, at least 97%, at least 97.5%, at least 98%, at least 98.5%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to one or more of SEQ ID NO's 1 to 44.

In an embodiment, a non-inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 44, or a nucleotide sequence at least 95% identical to one or more of SEQ ID NO's 1 to 44.

In an embodiment, a non-inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 44, or a nucleotide sequence at least 96% identical to one or more of SEQ ID NO's 1 to 44.

In an embodiment, a non-inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 44, or a nucleotide sequence at least 97% identical to one or more of SEQ ID NO's 1 to 44.

In an embodiment, a non-inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 44, or a nucleotide sequence at least 98% identical to one or more of SEQ ID NO's 1 to 44.

In an embodiment, a non-inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 44, or a nucleotide sequence at least 99% identical to one or more of SEQ ID NO's 1 to 44.

In an embodiment, a non-inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 44, or a nucleotide sequence at least 99.5% identical to one or more of SEQ ID NO's 1 to 44.

In an embodiment, an inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 90%, at least 90.5%, at least 91%, at least 91.5%, at least 92%, at least 92.5%, at least 93%, at least 93.5%, at least 94%, at least 94.5%, at least 95%, at least 95.5%, at least 96%, at least 96.5%, at least 97%, at least 97.5%, at least 98%, at least 98.5%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to one or more of SEQ ID NO's 45 to 74.

In an embodiment, an inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 94%, at least 94.5%, at least 95%, at least 95.5%, at least 96%, at least 96.5%, at least 97%, at least 97.5%, at least 98%, at least 98.5%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to one or more of SEQ ID NO's 45 to 74.

In an embodiment, an inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 95% identical to one or more of SEQ ID NO's 45 to 74.

In an embodiment, an inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 96% identical to one or more of SEQ ID NO's 45 to 74.

In an embodiment, an inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 97% identical to one or more of SEQ ID NO's 45 to 74.

In an embodiment, an inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 98% identical to one or more of SEQ ID NO's 45 to 74.

In an embodiment, an inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 99% identical to one or more of SEQ ID NO's 45 to 74.

In an embodiment, an inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 99.5% identical to one or more of SEQ ID NO's 45 to 74.

In an embodiment, an inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 97% identical to nucleic acid residues 1100 to 1210 of SEQ ID NO's 45 to 74.

In an embodiment, an inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 98% identical to nucleic acid residues 1100 to 1210 of SEQ ID NO's 45 to 74.

In an embodiment, an inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 99.5% identical to nucleic acid residues 1100 to 1210 of SEQ ID NO's 45 to 74.

In an embodiment, an inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 100% identical to nucleic acid residues 1100 to 1210 of SEQ ID NO's 45 to 74.

In an embodiment, an inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 100% identical to nucleic acid residues 1100 to 1210 of SEQ ID NO's 45 to 50.

In an embodiment, an inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 100% identical to nucleic acid residues 1100 to 1210 of SEQ ID NO 45.

In an embodiment, an inflammatory strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in SEQ ID NO:45, or a nucleotide sequence at least 90%, at least 90.5%, at least 91%, at least 91.5%, at least 92%, at least 92.5%, at least 93%, at least 93.5%, at least 94%, at least 94.5%, at least 95%, at least 95.5%, at least 96%, at least 96.5%, at least 97%, at least 97.5%, at least 98%, at least 98.5%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to identical to SEQ ID NO:45.

In an embodiment, a strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 74, or a nucleotide sequence at least 90%, at least 90.5%, at least 91%, at least 91.5%, at least 92%, at least 92.5%, at least 93%, at least 93.5%, at least 94%, at least 94.5%, at least 95%, at least 95.5%, at least 96%, at least 96.5%, at least 97%, at least 97.5%, at least 98%, at least 98.5%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to one or more of SEQ ID NO's 1 to 74.

In an embodiment, a strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 74, or a nucleotide sequence at least 90%, at least 90.5%, at least 91%, at least 91.5%, at least 92%, at least 92.5%, at least 93%, at least 93.5%, at least 94%, at least 94.5%, at least 95%, at least 95.5%, at least 96%, at least 96.5%, at least 97%, at least 97.5%, at least 98%, at least 98.5%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to one or more of SEQ ID NO's 1 to 74, wherein the 16s ribosomal RNA (rRNA) gene does not comprise any one of SEQ ID NO's 89 to 217. Alternatively, the 16s ribosomal RNA (rRNA) gene does comprise any one of SEQ ID NO's 89 to 217.

In an embodiment, a strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 44, or a nucleotide sequence at least 90%, at least 90.5%, at least 91%, at least 91.5%, at least 92%, at least 92.5%, at least 93%, at least 93.5%, at least 94%, at least 94.5%, at least 95%, at least 95.5%, at least 96%, at least 96.5%, at least 97%, at least 97.5%, at least 98%, at least 98.5%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to one or more of SEQ ID NO's 1 to 44, wherein the 16s ribosomal RNA (rRNA) gene does not comprise any one of SEQ ID NO's 89 to 217. Alternatively, the 16s ribosomal RNA (rRNA) gene does comprise any one of SEQ ID NO's 89 to 217.

In an embodiment, a strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 74, or a nucleotide sequence at least 90%, at least 90.5%, at least 91%, at least 91.5%, at least 92%, at least 92.5%, at least 93%, at least 93.5%, at least 94%, at least 94.5%, at least 95%, at least 95.5%, at least 96%, at least 96.5%, at least 97%, at least 97.5%, at least 98%, at least 98.5%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to one or more of SEQ ID NO's 45 to 74, wherein the 16s ribosomal RNA (rRNA) gene does not comprise any one of SEQ ID NO's 89 to 217. Alternatively, the 16s ribosomal RNA (rRNA) gene does comprise any one of SEQ ID NO's 89 to 217.

In an embodiment, a strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 45 to 50, or a nucleotide sequence at least 90%, at least 90.5%, at least 91%, at least 91.5%, at least 92%, at least 92.5%, at least 93%, at least 93.5%, at least 94%, at least 94.5%, at least 95%, at least 95.5%, at least 96%, at least 96.5%, at least 97%, at least 97.5%, at least 98%, at least 98.5%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to one or more of SEQ ID NO's 45 to 50, wherein the 16s ribosomal RNA (rRNA) gene does not comprise any one of SEQ ID NO's 89 to 217. Alternatively, the 16s ribosomal RNA (rRNA) gene does comprise any one of SEQ ID NO's 89 to 217.

In an embodiment, a strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence of SEQ ID NO 45, or a nucleotide sequence at least 90%, at least 90.5%, at least 91%, at least 91.5%, at least 92%, at least 92.5%, at least 93%, at least 93.5%, at least 94%, at least 94.5%, at least 95%, at least 95.5%, at least 96%, at least 96.5%, at least 97%, at least 97.5%, at least 98%, at least 98.5%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to SEQ ID NO 45, wherein the 16s ribosomal RNA (rRNA) gene does not comprise any one of SEQ ID NO's 89 to 217. Alternatively, the 16s ribosomal RNA (rRNA) gene does comprise any one of SEQ ID NO's 89 to 217.

In an embodiment, the % identity of a polynucleotide is determined by GAP (Needleman and Wunsch, 1970) analysis (GCG program) with a gap creation penalty=5, and a gap extension penalty=0.3. Preferably, the GAP analysis aligns two sequences over their entire length.

The bacterial strains for use in the present invention can be cultured using standard microbiology techniques as detailed in, for instance, Handbook of Microbiological Media, Fourth Edition (2010) Ronald Atlas, CRC Press, Maintaining Cultures for Biotechnology and Industry (1996) Jennie C. Hunter-Cevera, Academic Press, as well as how detailed in the Examples using YCFA medium.

Methods Treatment or Prevention

Methods of the invention can be used to treat or prevent a dysbiosis of the gastrointestinal tract in a subject. "Dysbiosis" in the context of the present invention refers to a state in which the normal diversity and/or function of the microbiota or microbiome, in particular the human gastrointestinal microbiota, is disrupted. Any disruption from the normal state of the microbiota in a healthy individual can be considered a dysbiosis, even if the dysbiosis does not result in a detectable decrease in health in the individual. In a preferred embodiment, the dysbiosis may be associated with one or more pathological symptoms. For example, "dysbiosis" may refer to a decrease in the microbial diversity of the microbiota. In addition, or alternatively, "dysbiosis" may refer to an increase in the abundance of one or more bacteria, e.g. one or more pathogenic bacteria, in the microbiota of an individual relative to the abundance of said bacterium or bacteria in the microbiota of a healthy individual, i.e. an individual without a dysbiosis. The pathogenic bacteria present during dysbiosis are often Proteobacteria and resistant to one or more antibiotics. Examples of Proteobacteria include Escherichia, Salmonella, Campylobacter, Vibrio, Helicobacter, and Yersinia species.

The dysbiosis may be a dysbiosis associated with an enteric bacterial infection, such as an infection of the gastrointestinal tract with a pathogenic bacterium. Many bacteria capable of causing infections of the gastrointestinal tract in humans are known and include: gram positive bacteria, and gram negative bacteria. The pathogenic bacterium is preferably a pathogenic species of the genus Clostridium, Escherichia, Enterococcus, Klebsiella, Enterobacter, Proteus, Salmonella, Shigella, Staphylococcus, Vibrio, Aeromonas, Campylobacter, Plesiomonas, Bacillus, Helicobacter, Listeria, or Yersinia. Preferred examples of such pathogenic bacteria include Clostridium difficile, Clostridium perfringens, Clostridium botulinum, Escherichia coli, Salmonella typhi, Staphylococcus aureus, Vibrio cholerae, Vibrio parahaemolyticus, Vibrio vulnificus, Campylobacter fetus, Campylobacter jejuni, Aeromonas hydrophila, Plesiomonas shigelloides, Bacillus cereus, Helicobacter pylori, Listeria monocytogenes, and Yersinia enterocolitica. More preferably, the pathogenic bacterium is a pathogenic species of the genus Clostridium or Escherichia. Most preferably, the pathogenic bacterium is Clostridium difficile or Escherichia coli. Methods of the invention can be used to reduce or prevent gastrointestinal disorders.

Methods of the invention can be used to reduce or prevent gastrointestinal tract mucosal inflammation in a subject using a non-inflammatory (non-cell cytotoxic) strain of Enterococcus sp. of the invention.

In an embodiment, the subject has, or is susceptible to having, an inflammatory bowel diseases (IBD) such as Crohn's disease, ulcerative colitis or pouchitis. As used herein, the term "inflammatory bowel diseases (IBD)" has its general meaning in the art and refers to a group of inflammatory diseases of the colon and small intestine such as revised in the World Health Organisation Classification K20-K93 (ICD-10) such as Crohn disease (such as granulomatous enteritis; Crohn disease of small intestine; Crohn disease of large intestine; granulomatous and regional Colitis; Crohn disease of colon, large bowel and rectum; Crohn disease of both small and large intestine), Ulcerative colitis (such as Ulcerative (chronic) pancolitis; backwash ileitis; Ulcerative (chronic) proctitis; Ulcerative (chronic) rectosigmoiditis; Inflammatory polyps; Left sided colitis; left hemicolitis) and noninfective gastroenteritis and colitis (Gastroenteritis and colitis due to radiation; Toxic gastroenteritis and colitis; Allergic and dietetic gastroenteritis and colitis; Food hypersensitivity gastroenteritis or colitis; indeterminate colitis; specified noninfective gastroenteritis and colitis such as Collagenous colitis; Eosinophilic gastritis or gastroenteritis; Lymphocytic colitis Microscopic colitis (collagenous colitis or lymphocytic colitis); Noninfective gastroenteritis and colitis such as Diarrhoea; Enteritis; Ileitis; Jejunitis; Sigmoiditis) and postprocedural disorders of digestive system such as pouchitis. In an embodiment, the IBD is paediatric IBD.

In a further aspect, the present invention also relates to a fecal microbiota transplant composition comprising the strain of the invention. The term "fecal microbiota transplant composition" has its general meaning in the art and refers to any composition that can restore the fecal microbiota.

In a further aspect, there is a method of decreasing the growth of pathogenic bacteria, said method comprising the step of administering to a patient in need thereof, a composition comprising a non-inflammatory (non-cell cytotoxic) strain of Enterococcus sp. of the invention. In a preferred embodiment, the method reduces the relative abundance of pathogenic bacterium or decolonises pathogenic bacterium.

In a further aspect, there is a method of increasing the growth of healthy bacteria, said method comprising the step of administering to a patient in need thereof, a composition comprising a non-inflammatory (non-cell cytotoxic) strain of Enterococcus sp. of the invention.

Administration to humans includes administration by a medical professional and self-administration. In general, in order to achieve a health benefit, multiple doses of the biotherapeutic composition are administered, for example daily for a period of at least one week, at least two weeks, at least three weeks, at least six weeks, at least nine weeks, or at least twelve weeks. In one embodiment, the biotherapeutics can be administered for the remaining duration of a subject's life.

In a further embodiment, the composition is administered to the patient using a dosing regimen selected from the group consisting of: hourly; every 2 hours; every 3 hours; every 4 hours; every 5 hours; every 6 hours; every 12 hours; once daily; twice daily; every 2 days; every 3 days; every 4 days; every 5 days; every 6 days; weekly; twice weekly; every 2 weeks; every 3 weeks; every 4 weeks; every 5 weeks; every 6 weeks; once monthly; twice monthly; every 2 months; every 3 months; every 4 months; every 5 months; every 6 months; yearly; twice yearly; every 2 years; every 3 years; every 4 years; and every 5 years.

In an embodiment, the method reduces the relative abundance of a member of the bacteria genus or decolonises a member of the bacteria genus. Preferably, the method drives down undesired inflammation. Preferably, the said method decreases inflammation in the subject when measured by a parameter selected from the group consisting of: TNFα signalling via NF-κB; IFNα signalling; IFNγ signalling; IL6 JAK STAT3 signalling; activation of pro-apoptotic pathways; initiation of unfolded protein response. Preferably, the said method upregulates genes associated with pro- apoptotic pathways and the unfolded protein response, including genes selected from the group consisting of: CHAC1 , CEBPB, TRIB3, PPP1 R15A, DDIT3, ATF4 and XBP1.

Preferably, the composition is administered orally or rectally.

Compositions

The therapeutic composition of the invention may comprise a pharmaceutically acceptable excipient, carrier, buffer, stabilizer or other materials well known to those skilled in the art. Such materials should be non-toxic and should not interfere with the efficacy of the isolated bacteria present in the therapeutic composition. The precise nature of the pharmaceutically acceptable excipient or other material will depend on the route of administration, which may be, for example, oral or rectal. Many methods for the preparation of therapeutic compositions are known to those skilled in the art (see e.g. Robinson ed., Sustained and Controlled Release Drug Delivery Systems, Marcel Dekker, Inc., New York, 1978).

The therapeutic composition of the invention may comprise a prebiotic, a carrier, insoluble fibre, a buffer, an osmotic agent, an anti-foaming agent and/or a preservative.

The therapeutic composition may be made or provided in chemostat medium. Alternatively, the therapeutic composition may be made or provided in saline, e.g., 0.9% saline. It will be understood that any carrier or solution which does not impair viability of the bacteria present in the therapeutic composition and is compatible with administration to an individual may be used.

The therapeutic composition may be made or provided under reduced atmosphere, i.e., in the absence of oxygen. A synthetic stool preparation may be made or provided under N ₂ , CO ₂ , H ₂ , or a mixture thereof, optionally with controlled levels of partial pressure of N ₂ :CO ₂ :H ₂ .

The therapeutic composition may be for oral or rectal administration to the individual. Where the therapeutic composition is for oral administration, the therapeutic composition may be in the form of a capsule, or a tablet. Where the therapeutic composition is for rectal administration, the therapeutic composition may be in the form of an enema. The preparation of suitable capsules, tablets and enema is well-known in the art. The capsule or tablet may comprise a coating to protect the capsule or tablet from stomach acid. For example, the capsule or tablet may be enteric-coated, pH dependant, slow-release, and/or gastro-resistant. Such capsules and tablets are used, for example, to minimize dissolution of the capsule or tablet in the stomach but allow dissolution in the small intestine.

Orally dosed formulations, for example, can, in addition to the viable microorganisms comprise, inert compression aids, such as microcrystalline cellulose or oligosaccharide, flow aids, such as a silica gel, or a lubricant of, for example magnesium stearate (vegetable source) or stearic acid (vegetable source).

A composition disclosed herein can be used as, for example, a food supplement, an edible product or pharmaceutical product. When it is a food supplement, the biotherapeutic composition can further comprise a conventional food supplement filler and/or an extender. The biotherapeutic composition disclosed herein can also be included in any edible products, such as dairy products, including for example, a milk product, milk, yogurt, curd, ice-cream, dressing, and cheese, beverage products, meat products, and baked goods Suppository formulations, for example, either for rectal use, can in addition to the biotherapeutics, comprise, for example, cocoa butter, polyethylene glycol, glycerine or gelatine.

The composition may comprise a disintegrant, a glidant, and/or a lubricant. Disintegrants aid in the breakup of the compacted mass when placed in a fluid environment. The disintegrant may be any suitable disintegrant such as for example, a disintegrant selected from the group consisting of sodium croscarmellose, crospovidone, gellan gum, hydroxypropyl cellulose, starch, and sodium starch glycolate. The glidant may be any suitable glidant such as for example, a glidant selected from the group consisting of silicon dioxide, colloidal silicon dioxide, and talc. Lubricants are generally always used in the manufacture of dosage forms by direct compression in order to prevent the compacted powder mass from sticking to the equipment during the tabletting or encapsulation process. The lubricant may be any suitable lubricant such as for example, a lubricant selected from the group consisting of calcium stearate, magnesium stearate, stearic acid, sodium stearyl fumerate, and vegetable based fatty acids. In the composition and method of the present invention, the carrier, may be present in the composition in a range of approximately 30% w/w to approximately 98% w/w; this weight percentage is a cumulative weight percentage taking into consideration all ingredients present in the carrier.

Coatings can be used to control the solubility of the composition. Examples of coatings include carrageenan, cellulose acetate phthalate, ethylcelulose, gellan gum, matodextrin, methacrylates, methylcellulose, microcrystalline cellulose, and shellac.

The composition may comprise one or more preservatives. Exemplary preservatives include antioxidants, chelating agents, antifungal preservatives, alcohol preservatives, acidic preservatives, and other preservatives.

Exemplary antioxidants include alpha tocopherol, ascorbic acid, acorbyl palmitate, butylated hydroxyanisole, butylated hydroxytoluene, monothioglycerol, potassium metabi sulfite, propionic acid, propyl gallate, sodium ascorbate, sodium bisulfite, sodium metabi sulfite, and sodium sulfite.

Exemplary chelating agents include ethylenediaminetetraacetic acid (EDTA) and salts and hydrates thereof (e.g., sodium edetate, disodium edetate, trisodium edetate, calcium disodium edetate, dipotassium edetate, and the like), citric acid and salts and hydrates thereof (e.g., citric acid monohydrate), fumaric acid and salts and hydrates thereof, malic acid and salts and hydrates thereof, phosphoric acid and salts and hydrates thereof, and tartaric acid and salts and hydrates thereof. Exemplary antimicrobial preservatives include benzalkonium chloride, benzethonium chloride, benzyl alcohol, bronopol, cetrimide, cetylpyridinium chloride, chlorhexidine, chlorobutanol, chlorocresol, chloroxylenol, cresol, ethyl alcohol, glycerin, hexetidine, imidurea, phenol, phenoxyethanol, phenylethyl alcohol, phenylmercuric nitrate, propylene glycol, and thimerosal.

Exemplary antifungal preservatives include butyl paraben, methyl paraben, ethyl paraben, propyl paraben, benzoic acid, hydroxybenzoic acid, potassium benzoate, potassium sorbate, sodium benzoate, sodium propionate, and sorbic acid.

Exemplary alcohol preservatives include ethanol, polyethylene glycol, phenol, phenolic compounds, bisphenol, chlorobutanol, hydroxybenzoate, and phenylethyl alcohol.

Exemplary acidic preservatives include vitamin A, vitamin C, vitamin E, betacarotene, citric acid, acetic acid, dehydroacetic acid, ascorbic acid, sorbic acid, and phytic acid.

Other preservatives include tocopherol, tocopherol acetate, deteroxime mesylate, cetrimide, butylated hydroxyanisol (BHA), butylated hydroxytoluened (BHT), ethylenediamine, sodium lauryl sulfate (SLS), sodium lauryl ether sulfate (SLES), sodium bisulfite, sodium metabi sulfite, potassium sulfite, potassium metabi sulfite, Glydant Plus, Phenonip, methylparaben, Germall 115, Germaben II, NeoIone, Kathon, and EuxyL I.

The therapeutic composition may be a liquid.

The therapeutic composition may be lyophilized. The lyophilized therapeutic composition may comprise one or more stabilisers and/or cryoprotectants. The lyophilized therapeutic composition may be reconstituted using a suitable diluent prior to administration to the individual. Preferably, the cryoprotectant is selected from the group consisting of: trehalose; mannitol; sucrose; glycerol; sorbitol; DMSO; propylene glycol; ethylene glycol; saccharose; galactose-lactose; inulin; maltodextrin; and any combination thereof. Preferably, the said cryoprotectant further comprises a compound selected from the group consisting of: glycerol; polyethylene glycol (PEG); glycerin; erythritol; arabitol; xylitol; sorbitol; glucose; lactose; ribose; and any combination thereof. Preferably, the said cryoprotectant is trehalose at a concentration of 2% to 15% in said lyophilized formulation. Preferably, the said cryoprotectant is trehalose at a concentration of at least 5% in said lyophilized formulation. Preferably, the said cryoprotectant is trehalose at a concentration of at least 10% in said lyophilized formulation.

The composition of any one of claims X to Y, wherein the at least one strain of bacteria is diluted with an inert powdered diluent. In an embodiment, after at least 4 weeks of storage at room temperature, said composition is capable of maintaining at least 10% cell viability relative to the initial cell viability immediately prior to storage and pre-lyophilisation.

In an embodiment, after at least 4 weeks of storage at room temperature, said composition is capable of maintaining at least 50% cell viability relative to the initial cell viability immediately prior to storage and post-lyophilisation.

In an embodiment, after at least 4 weeks of storage at room temperature said composition is capable of maintaining about 60% to about 80% cell viability relative to the initial cell viability immediately prior to the start of said storage.

In an embodiment, said composition decreases inflammation in the subject when measured by a parameter selected from the group consisting of: TNFα signalling via NF-κB; IFNα signalling; IFNγ signalling; IL6 JAK STAT3 signalling; activation of pro-apoptotic pathways; initiation of unfolded protein response.

In an embodiment, said composition down regulates genes associated with pro- apoptotic pathways and the unfolded protein response, including genes selected from the group consisting of: CHAC1 , CEBPB, TRIB3, PPP1 R15A, DDIT3, ATF4 and XBP1.

In an embodiment, the bacteria is cultured from a faecal or colonic biopsy sample. In an embodiment, the bacteria comprises a community of bacterial cells derived from a stool or biopsy of one or more human donors. In an embodiment, the community of bacterial cells comprises cultured bacterial cells. In an embodiment, the cultured bacterial cells are derived from a multiple of human donors. In an embodiment, the community of bacterial cells comprises uncultured bacterial cells. In an embodiment, the uncultured bacterial cells are derived from a single human donor. In an embodiment, the composition is a faecal transplant microbiota composition.

A therapeutic composition according to the present invention may be administered alone or in combination with other treatments, concurrently or sequentially or as a combined preparation with another therapeutic agent or agents, for the treatment of dysbiosis, or a disease associated with dysbiosis as described herein. For example, a strain of the invention may be used in combination with an existing therapeutic agent for inflammatory bowel disease, irritable bowel syndrome, a metabolic disease, a neuropsychiatric disorder, an autoimmune disease, an allergic disorder, a cancer, or hepatic encephalopathy.

For example, where the therapeutic composition is for the treatment of a dysbiosis associated with cancer, the therapeutic composition may optionally be administered in combination with a cancer immunotherapy, such as an immune check- point inhibitor, to the individual. Examples of check-point inhibitors which may be employed in this context include Programmed cell death protein 1 (PD-1 ) inhibitors, Programmed death-ligand 1 (PD-L1) inhibitors, cytotoxic T- lymphocyte-associated protein 4 (CTLA-4) inhibitors. Manipulation of the gut microbiota in combination with immune check-point inhibitor treatment has been shown to improve efficacy of immune check-point inhibitors in treating cancer. In a preferred embodiment, the cancer in this context is lung cancer or melanoma.

In another embodiment, the composition of the invention further comprise immunomodulating compounds. In other embodiments, the immunomodulating compound is a cytokine, chemokine, or complement component that enhances expression of immune system accessory or adhesion molecules, their receptors, or combinations thereof. In some embodiments, the immunomodulating compound include interleukins, for example interleukins 1 to 15, interferons alpha, beta or gamma, tumour necrosis factor, granulocyte-macrophage colony stimulating factor (GM-CSF), macrophage colony stimulating factor (M-CSF), granulocyte colony stimulating factor (G-CSF), chemokines such as neutrophil activating protein (NAP), macrophage chemoattractant and activating factor (MCAF), RANTES, macrophage inflammatory peptides MIP-1a and MIP-1 b, complement components, or combinations thereof. In other embodiments, the immunomodulating compound stimulate expression, or enhanced expression of 0X40, OX40L (gp34), lymphotactin, CD40, CD40L, B7.1 , B7.2, TRAP, ICAM-1 , 2 or 3, cytokine receptors, or combination thereof.

In another embodiment, the immunomodulatory compound induces or enhances expression of co-stimulatory molecules that participate in the immune response, which include, in some embodiments, CD40 or its ligand, CD28, CTLA-4 or a B7 molecule. In another embodiment, the immunomodulatory compound induces or enhances expression of a heat stable antigen (HSA), chondroitin sulfate-modified MHC invariant chain (li-CS), or an intracellular adhesion molecule 1 (ICAM-1).

The therapeutic compositions of the invention may be administered to an individual, preferably a human individual. Administration may be in a "therapeutically effective amount", this being sufficient to show benefit to the individual. Such benefit may be at least amelioration of at least one symptom. Thus "treatment" of a specified disease refers to amelioration of at least one symptom. The actual amount administered, and rate and time-course of administration, will depend on the nature and severity of what is being treated, the particular patient being treated, the clinical condition of the individual patient, the cause of the dysbiosis, the site of delivery of the composition, the type of therapeutic composition, the method of administration, the scheduling of administration and other factors known to medical practitioners. Prescription of treatment, e.g. decisions on dosage etc., is within the responsibility of general practitioners and other medical doctors, and may depend on the severity of the symptoms and/or progression of a disease being treated. A therapeutically effective amount or suitable dose of a therapeutic composition of the invention can be determined by comparing its in vitro activity and in vivo activity in an animal model. Methods for extrapolation of effective dosages in mice and other test animals to humans are known. The precise dose will depend upon a number of factors, including whether the therapeutic composition is for prevention or for treatment.

Formulary ingredients can be contacted with a biotherapeutic preparation and mixed or prepared until a biotherapeutic formulation is obtained. As will be clear to those of skill in the art, formulation conditions will generally be such that viable microorganisms are retained. In particular high temperatures, for example temperatures in excess of 40°C are avoided.

The amount of viable microorganisms included in a biotherapeutic composition can vary and can be adjusted and optimized as will be appreciated by those of skill in the art Such optimization may, for example, be achieved by preparing a series of different doses of a viable microorganism. The bacterial concentration in the composition can be, for example, from 10 million cfu/mL to 100 billion cfu/mL, from 10 million to 50 million cfu/mL, more preferably from 50 million to 100 million cfu/mL, from 100 million to 500 million cfu/mL, from 500 million to 1 billion cfu/mL, from 1 billion to 5 billion cfu/mL, from 5 billion to 10 billion cfu/mL, from 10 billion to 15 billion cfu/mL, from 15 billion to 20 billion cfu/mL, from 20 billion to 25 billion cfu/mL, from 25 billion to 30 billion cfu/mL, from 30 billion to 35 billion cfu/mL, from 35 billion to 40 billion cfu/mL, from 40 billion to 45 billion cfu/mL, from 45 billion to 50 billion cfu/mL, from 50 billion to 55 billion cfu/mL, from 55 billion to 60 billion cfu/mL, from 60 billion to 65 billion cfu/mL, from 65 billion to 70 billion cfu/mL, from 70 billion to 75 billion cfu/mL, from 75 billion to 80 billion cfu/mL, from 80 billion to 85 billion cfu/mL, from 85 billion to 90 billion cfu/mL, from 90 billion to 95 billion cfu/mL, from 95 billion to 100 billion cfu/mL.

In an embodiment, the strain of the invention can be administered at, for example, a dosage of 0.01 to 100 x 10 ¹¹ cells/body, 0.1 to 10 x 10 ¹¹ cells/body or 0.3 to 5 x 10 ¹¹ cells/body. Furthermore, for example, the amount ingested per day as the bacteria can be 0.01 to 100 x 10 ¹¹ cells/60 kg body weight, 0.1 to 10 x 10 ¹¹ cells/60 kg body weight or 0.3 to 5 x 10 ¹¹ cells/60 kg body weight.

The content of the biotherapeutic contained in the orally ingested composition of the present invention may be determined as appropriate depending on its application form. As biotherapeutic dry microbial body it can be, for example, 5 to 50 w/w %, 1 to 75 w/w %, 0.1 to 100 w/w % or 1 to 100 w/w %.

In an embodiment, every 200 mg of the composition comprises a pharmacologically active dose of bacteria cells or spores selected from the group consisting of: 10 ³ to 10 ¹⁴ ; 10 ⁴ to 10 ¹⁴ ; 10 ⁵ to 10 ¹⁴ ;10 ⁶ to 10 ¹⁴ ; 10 ⁷ to 10 ¹⁴ ; 10 ⁸ to 10 ¹⁴ ; 10 ⁴ to 10 ¹³ ; 10 ⁵ to 10 ¹² ; 10 ⁶ to 10 ¹¹ ; 10 ⁷ to 10 ¹ °; 10 ⁸ to 10 ⁹ ; 10 ³ to 10 ¹³ ; 10 ³ to 10 ¹² ; 10 ³ to 10 ¹¹ ; 10 ³ to 10 ¹ °; 10 ³ to 10 ⁹ ; 10 ³ to 10 ⁸ ; 10 ³ to 10 ⁷ ; 10 ³ to 10 ⁶ ; 10 ³ to 10 ⁵ , and 10 ³ to 10 ⁴ colony forming units (cfu) or total cell count.

In an embodiment, the composition comprises a pharmacologically active dose of bacteria cells or spores selected from the group consisting of: from 10 million cfu/mL to 100 billion cfu/mL, from 10 million to 50 million cfu/mL, more preferably from 50 million to 100 million cfu/mL, from 100 million to 500 million cfu/mL, from 500 million to 1 billion cfu/mL, from 1 billion to 5 billion cfu/mL, from 5 billion to 10 billion cfu/mL, from 10 billion to 15 billion cfu/mL, from 15 billion to 20 billion cfu/mL, from 20 billion to 25 billion cfu/mL, from 25 billion to 30 billion cfu/mL, from 30 billion to 35 billion cfu/mL, from 35 billion to 40 billion cfu/mL, from 40 billion to 45 billion cfu/mL, from 45 billion to 50 billion cfu/mL, from 50 billion to 55 billion cfu/mL, from 55 billion to 60 billion cfu/mL, from 60 billion to 65 billion cfu/mL, from 65 billion to 70 billion cfu/mL, from 70 billion to 75 billion cfu/mL, from 75 billion to 80 billion cfu/mL, from 80 billion to 85 billion cfu/mL, from 85 billion to 90 billion cfu/mL, from 90 billion to 95 billion cfu/mL, from 95 billion to 100 billion cfu/mL.

In an embodiment, the composition comprises a pharmacologically active dose of bacteria cells or spores wherein the concentration of the bacteria cells or spores as a dry microbial body, is selected from the group consisting of: between 5 to 50 w/w %, 1 to 75 w/w %, 0.1 to 100 w/w % and 1 to 100 w/w %.

In an embodiment, the composition is a controlled release composition. As used herein, the term "controlled-release" refers to release or administration of a strain of the invention from a given dosage form in a controlled fashion in order to achieve the desired pharmacokinetic profile in vivo. An aspect of "controlled" delivery is the ability to manipulate the formulation and/or dosage form in order to establish the desired kinetics of biotherapeutic release.

Procedures for preparing tablets, caplets, capsules and other forms of compositions of the invention are known to those of ordinary skill in the art and include without limitation wet granulation, dry granulation, and direct compression (for tablets and caplets). Wet and dry granulation is used to manufacture tablets, caplets, or capsules. With granulation techniques, a chilsonation is used to manufacture the powder for the dosage forms. A chilsonator houses grooved, rotating rollers that are pressed tightly against one another by hydraulic pressure. Raw materials are placed into the hopper of the chilsonator and are fed by a system of horizontal and vertical screws into the rollers. As materials pass through the grooves in the rollers, it is compacted under very high pressure and emerges from the chilsonator as dense sheets. The sheets are milled into a fine granular powder using a Fitz mill and then passed through a screen to produce a uniform free flowing granule. The chilsonation process results in a finished powder that is two to four times denser than the starting material, a feature that permits the ingredients to be fashioned into the desired dosage form.

With dry granulation, the powder may be incorporated into a gelatin capsule or it may be mixed with gelatin to form a tablet or caplet. With wet granulation, the powder is moistened thus creating large "chunks" of material that are subsequently dried and milled to convert the chunks to particles of a desired size for the manufacturing process. Once the particles of a desired size are obtained, the particles are incorporated into a gelatin capsule or mixed with gelatin to form a tablet or caplet.

General considerations in formulation and/or manufacture can be found, for example, in Remington's Pharmaceutical Sciences, Sixteenth Edition, E. W. Martin (Mack Publishing Co., Easton, Pa., 1980), and Remington: The Science and Practice of Pharmacy, 21st Edition (Lippincott Williams & Wilkins, 2005).

In a further aspect, there is a method of preparing a composition of this invention, said method comprising culturing at least one biotherapeutic strain of Enterococcus sp and mixing the at least one strain with a pharmaceutically acceptable carrier.

Prebiotics

A composition of the invention can comprise a prebiotic. Because prebiotics have a chemical structure that resists digestion through the alimentary tract, they reach the colon as intact molecules where they are able to elicit systemic physiological functions and act as fermentable substrates for colonic microflora. Where a prebiotic is combined with a biotherapeutic, the resulting composition is sometimes referred to as a "synbiotic."

Examples of suitable prebiotics include, but are not limited to, oligosaccharide such as fructooligosaccharides, P95 Nutraflora®, for example, galactooligosaccharides, xylooligosaccharides, isomaltooligosaccharides, human milk oligosaccharides, inulin oligosaccharides, mannan oligosaccharides, pyrodextrin, levan, maltotriose, pectic oligosaccharides, bimuno-galactooligosaccharides, arabinoxylan, and fucoidan. Fructooligosaccharides can be extracted from, for example, chicory, artichokes, asparagus, dandelions, dahlias, endive, garlic, leeks, lettuce, and onions.

In an embodiment, the prebiotic comprises amino acids such as one or more or all of alanine, aspartic acid, glutamic acid, glycine, leucine, isoleucine, proline, serine, threonine and valine.

In an embodiment, the prebiotic comprises simple sugars which can be a monosaccharide (such as glucose, galactose or fructose) and/or a disaccharide (such as sucrose maltose or lactose).

In an embodiment, the prebiotic comprises from about 5% (w/w) to about 50% (w/w), about 7.5% (w/w) to about 30% (w/w) or about 10% (w/w) to about 15% (w/w) of the composition.

Other Microorganisms

In order to obtain the desired health benefit to the subject, it may be advantageous to include one or more additional biotherapeutic microorganisms in the composition. Thus, the composition may comprise more than one species/strain of microorganisms in addition to the strain of the invention, such as two, three, four, five or a higher plurality of species/strains of microorganisms. Non-limiting examples of biotherapeutics are suitable strains of the genus Aerococcus, Adlercreutzia, Allobaculum, Bacillus, Bifidobacterium, Carnobacterium, Clostridium, Eubacterium, Enterococcus (in addition to an Enterococcus strain of the invention), Oenococcus, Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, Propionibacterium, Sporolactobacillus, Staphylococcus, Streptococcus and Tetragenococcus, Vagococcus and Weisella. It is to be understood that the foregoing list is intended only to be illustrative and not a limiting representation of the biotherapeutics that may be included in the composition of the present invention. In this respect, any additional biotherapeutic species may also be used in the compositions of the present invention.

In an embodiment, the Enterococcus sp. is Enterococcus faecalis or Enterococcus faecium.

In an embodiment, the Lactobacillus sp. is selected from the group consisting of Lactobacillus rhamnosus (such as strain GG (ATCC 53103), CGMCC 1.3724 or SP1 (DSM 21690)), Lactococcus lactis, Lactococcus cremoris, Lactococcus diacetylactis, Lactobacillus paracasei, Lactobacillus reuteri (such as strain ATCC 55730 or DSM 17938), Lactobacillus acidophilus, Lactobacillus murinus, Lactobacillus helveticus, Lactobacillus bulgaricus, Lactobacillus casei, Lactobacillus salivarius, Lactobacillus plantarum, Lactobacillus fermentum, Lactobacillus taiwanensis, Lactobacillus animalis, Lactobacillus johnsonii (such as strain NCC533; CNCM 1 -1225) and Lactobacillus gasseri.

In an embodiment, the Bifidobacterium sp. is selected from the group consisting of Bifidobacterium lactis (such as strain BB-12, BI-04 or CNCM 1-3446 (Bb12)), Bifidobacterium longum (such as strain NCC3001 , ATCC BAA-999 (BB536)), Bifidobacterium breve (such as strain Bb-03, M-16V or R0070), Bifidobacterium infantis, Bifidobacterium animalis, Bifidobacterium bifidum and Bifidobacterium adolescentis.

In an embodiment, the Streptococcus sp. is Streptococcus thermophilus such as Streptococcus thermophilus ST-21

In an embodiment, the Clostridium sp. is Clostridium difficile.

Some yeasts are also useful as biotherapeutics and are sometimes included in the biotherapeutic compositions. One non-limiting example of a yeast used in biotherapeutics is Saccharomyces boulardii.

Dosage Forms and Kits

In a further aspect of the invention, there is a dosage form comprising the composition according to an earlier aspect of the invention.

In a further aspect of the invention, there a kit comprising the dosage form together with instructions for its use.

Uses

In a further aspect of the invention, there is the use of the composition according to an earlier aspect of the invention in the manufacture of a medicament for reducing or preventing a gastrointestinal disorder in a subject.

Methods of Detection

A strain of the invention can be detected using a wide variety of known techniques. Conveniently the strain is detected using a nucleic acid based detection system.

In an embodiment, nucleic acid sequencing is used. Illustrative non-limiting examples of nucleic acid sequencing techniques include, but are not limited to, chain terminator (Sanger) sequencing and dye terminator sequencing. In some embodiments, the technology provided herein finds use in a Second Generation (a.k.a. Next Generation or Next-Gen), Third Generation (a.k.a. Next-Next-Gen), or Fourth Generation (a.k.a. N3-Gen) sequencing technology including, but not limited to, pyrosequencing, sequencing-by-ligation, single molecule sequencing, sequence-by- synthesis (SBS), massive parallel clonal, massive parallel single molecule SBS, massive parallel single molecule real-time, massive parallel single molecule real-time nanopore technology.

In some embodiments, hybridization is employed in a detection method of the invention. Illustrative non-limiting examples of nucleic acid hybridization techniques include, but are not limited to, in situ hybridization (ISH), microarray, and Southern or Northern blot. In one embodiment, a FISH assay is used.

In other embodiments, nucleic acid amplification is used. Nucleic acids may be amplified prior to or simultaneous with detection. Conducting one or more amplification reactions may comprise one or more PCR-based amplifications, non-PCR based amplifications, or a combination thereof. Illustrative non-limiting examples of nucleic acid amplification techniques include, but are not limited to, polymerase chain reaction (PCR), reverse transcription polymerase chain reaction (RT-PCR), nested PCR, linear amplification, multiple displacement amplification (MDA), real-time SDA, rolling circle amplification, circle-to-circle amplification transcription-mediated amplification (TMA), ligase chain reaction (LCR), strand displacement amplification (SDA), and nucleic acid sequence based amplification (NASBA). Those of ordinary skill in the art will recognize that certain amplification techniques (e.g., PCR) require that RNA be reversed transcribed to DNA prior to amplification (e.g., RT-PCR), whereas other amplification techniques directly amplify RNA (e.g., TMA and NASBA).

Non-amplified or amplified nucleic acids can be detected by any conventional means. For example, the nucleic acids can be detected by hybridization with a detectably labeled probe and measurement of the resulting hybrids. In another example, the nucleic acids are detected by sequencing. Illustrative non-limiting examples of detection methods are described herein.

Evaluation of an amplification process in "real-time" involves determining the amount of amplicon in the reaction mixture either continuously or periodically during the amplification reaction, and using the determined values to calculate the amount of target sequence initially present in the sample. A variety of methods for determining the amount of initial target sequence present in a sample based on real-time amplification are well known in the art. These include methods disclosed in U.S. Pat. Nos. 6,303,305 and 6,541 ,205. Another method for determining the quantity of target sequence initially present in a sample, but which is not based on a real-time amplification, is disclosed in U.S. Pat. No. 5,710,029.

Amplification products may be detected in real-time through the use of various self-hybridizing probes, most of which have a stem-loop structure. Such selfhybridizing probes are labelled so that they emit differently detectable signals, depending on whether the probes are in a self-hybridized state or an altered state through hybridization to a target sequence. By way of non-limiting example, "molecular torches" are a type of self-hybridizing probe that includes distinct regions of selfcomplementarity (referred to as "the target binding domain" and "the target closing domain") which are connected by a joining region (e.g., non-nucleotide linker) and which hybridize to each other under predetermined hybridization assay conditions. In a preferred embodiment, molecular torches contain single-stranded base regions in the target binding domain that are from 1 to about 20 bases in length and are accessible for hybridization to a target sequence present in an amplification reaction under strand displacement conditions. Under strand displacement conditions, hybridization of the two complementary regions, which may be fully or partially complementary, of the molecular torch is favored, except in the presence of the target sequence, which will bind to the single-stranded region present in the target binding domain and displace all or a portion of the target closing domain. The target binding domain and the target closing domain of a molecular torch include a detectable label or a pair of interacting labels (e g., luminescent/quencher) positioned so that a different signal is produced when the molecular torch is self-hybridized than when the molecular torch is hybridized to the target sequence, thereby permitting detection of probe:target duplexes in a test sample in the presence of unhybridized molecular torches. Molecular torches and a variety of types of interacting label pairs are disclosed in U.S. Pat. No. 6,534,274, herein incorporated by reference in its entirety.

Another example of a detection probe having self-complementarity is a "molecular beacon." Molecular beacons include nucleic acid molecules having a target complementary sequence, an affinity pair (or nucleic acid arms) holding the probe in a closed conformation in the absence of a target sequence present in an amplification reaction, and a label pair that interacts when the probe is in a closed conformation. Hybridization of the target sequence and the target complementary sequence separates the members of the affinity pair, thereby shifting the probe to an open conformation. The shift to the open conformation is detectable due to reduced interaction of the label pair, which may be, for example, a fluorophore and a quencher (e.g., DABCYL and EDANS). Molecular beacons are disclosed in U.S. Pat. Nos. 5,925,517 and 6,150,097.

In an embodiment, the method includes quantifying the amount of strain present in the sample.

In a further aspect, there is a method of analysing the microbiome of the gastrointestinal tract of a subject, the method comprising; obtaining a sample comprising bacteria from the gastrointestinal tract of the subject, analysing the sample for the presence of at least one strain of a biotherapeutic strains of Enterococcus sp.

In a further aspect, there is a method of analysing the microbiome of the gastrointestinal tract of a subject, the method comprising; obtaining a sample comprising bacteria from the gastrointestinal tract of the subject, analysing the sample for the presence of at least one strain of a pathogenic bacteria.

EXAMPLES

EXAMPLE 1 - Materials and Methods

Sample Collection

Fresh mucosal samples were obtained during paediatric endoscopy lists, from consenting patients receiving clinically indicated colonoscopies. Six mucosal samples were obtained from each patient, with paired samples collected from three intestinal regions (Terminal Ileum, Caecum, Rectum), to allow for matched mucosal culturing and transcriptional profiling. The samples for bacterial culturing were placed into anaerobic conditions within 15 minutes of collection. The samples for RNA sequencing were collected in 200 μl of RNAIater stabilisation solution (QIAGEN; Hilden, Germany) and stored at -80°C.

Bacterial Culturing

Sample processing took place under anaerobic conditions in a Whitley A95 workstation (Don Whitley Scientific; Yorkshire, United Kingdom) at 37°C. All reagents needed for bacterial culturing were pre-reduced in the anaerobic environment 24 hours prior to sample processing. To process, the samples were weighed, diluted by a factor of 10 with sterile pre-reduced PBS, serially diluted down to 10 ^-6 and plated directly onto YCFA agar (see WO 2021/163758). Samples were then grown in anaerobic, microaerophilic and aerobic conditions. The plates for culturing of anaerobic bacteria were incubated at 37°C in the Whitely A95 anaerobic workstation (Don Whitley Scientific; Yorkshire, United Kingdom), containing 10% carbon dioxide, 10% hydrogen and 80% nitrogen. The plates for culturing of aerobic bacteria were incubated at 37°C in the Bio concept incubator (Froilabo; Lionel Terray, Meyzieu, France). The plates for culturing of microaerophilic bacteria were stored in 2.5L gas jars (Thermo Scientific; Waltham, Massachusetts, United States) containing 2.5L CampyGen gas packs (Oxoid; Basingstoke, Hampshire, United Kingdom), and incubated at 37°C in the Bio concept incubator (Froilabo). Bacterial colony picking was performed 24 hours after plating, using plates harbouring distinct, non-converging bacterial colonies. To culture for metagenomic analysis, 50 μl aliquots of each dilution factor were applied to YCFA agar plates and uniformly spread across the plate using a disposable plate spreader (International Scientific Group). The plates were incubated at 37°C, in the appropriate environment. The plates were scraped for metagenomic analysis 24 hours after plating, using plates harbouring distinct, non-converging bacterial colonies.

Bacterial Purification and Archiving

Isolates were restreaked onto YCFA agar plates three times, at 24-hour intervals, for purification. Following purification, individual colonies were transferred into 15 ml Falcon tubes containing 14 ml of YCFA broth and incubated at 37°C for 24 hours. Pure bacterial isolates were archived at -80°C in cryogenic storage tubes, containing 25% (v/v) sterile glycerol.

Bacterial Identification

Identification of the picked isolates was performed through 16S rRNA gene amplification via PCR, followed by capillary sequencing. Broad range bacterial 16S gene primers were used, including a forward 7F primer: 5'- AGAGTTTGATYMTGGCTCAG-3' (SEQ ID NO:87), and a reverse 151 OR primer: 5'- ACGGYTACCTTGTTACGACTT-3' (SEQ ID NO:88). Following sequencing, a BLASTn search was performed, using SILVA 138.1 , to define each isolate as either a previously characterised or candidate novel species, with 97% sequence similarity between operational taxonomic units (OTUs) designated as the species-level cut-off.

Genomic Sequencing

A selection of 463 phylogenetically diverse representative isolates were selected for whole genome sequencing. DNA was extracted using the MP Biomedicals FastDNA SPIN Kit for soil, and sequenced on either the Illumina NextSeq2000 or the Illumina NextSeq550. Sequenced reads were trimmed using Trimmomatic v 0.38 and assembled using SPAdes v 3.13.0. Assembled genomes underwent quality control, looking for completeness (> 95%) and contamination (<5%) using CheckM v 1.1.3 (Parks et al., 2014). Genomes which passed quality control were annotated using Prokka v1 .13 (Seeman, 2014).

Metagenomic Sequencing and Genome Comparisons

DNA was also extracted from the whole metagenomic community scraped from a non-confluent agar plate, using the same DNA isolation process.

For candidate genomes of interest, genome annotation files were used as input into Roary v3.13.0 (Page et al. 2015) to identify and align core genes within groups of genomes. Core gene alignments were used to generate maximum likelihood trees using RAxML v8.2.11 with the GTR GAMMA model (Stamatakis, 2014). Trees were visualized using the interactive tree of life (iTOL). Inter-clade differences in gene presence were determined using the Roary output and examining the data for genes present in every strain of the selected clade and absent from any other strain. The presence of virulence genes within genomes was determined using diamond (Buchfink, et al., 2021) with the VFDB (Liu, et aL 2019) list of virulence proteins, and a percent identity cut-off of> 70%.

RNA Sequencing

RNA was extracted from both patient derived mucosal samples and Caco2 cells post stimulation, using the QIAGEN RNeasy Mini Kit as per the protocol, modified to encompass use of the FastPrep96 high-throughput homogenizer (MP Biomedicals), for the mucosal samples. For sequencing, libraries were generated using an in-house multiplex RNA-seq method (MHTP, Medical Genomics Facility), and paired-end sequencing (R1 19 bp; R2 72 bp) was performed on the NextSeq 550 (Illumina), using the v2.5 High Output Kit. Bcl2fastq (v2.20.0.422, Illumina) was used for base calling.

Patient RNA Sequencing Analysis

RNA sequencing analysis was performed in R (v3.5.1 ). The scPipe package (v1.2.1) was employed to process and de-multiplex the data. Read alignment was performed using the RSubread package (v1 .30.9). An index was built using the Ensembl Homo sapiens GRCh38 primary assembly genome file and alignment was performed with default settings. Aligned reads were mapped to exons using the sc_exon_mapping function with the Ensembl Homo sapiens GRCh38 v98 GFF3 genome annotation file. The resulting BAM file was de-multiplexed and reads mapping to exons were associated with each individual sample using the sc_demultiplex function, and an overall count for each gene for each was sample was generated using the sc_gene_counting function (with UMI cor = 1). Additional gene annotation was obtained using the biomaRt package (v2.36.1 ) and a DGEList object was created with the counts and gene annotation using the edgeR package (v3.28.1).

Gene set collections were obtained from the Broad Institute Molecular Signature Database (v5.2) using the EGSEA package (v1.14.0). The HALLMARK INFLAMMATORY RESPONSE gene set from the Hallmark gene set collection was used to classify samples as inflamed (IR) or non-inflamed (NR), logs CPM expression values for the 166 detected genes inflammatory response genes were used for k-means clustering using the kmeans function from the R stats package with two centres and 100 random starts. Multidimensional scaling plots were generated on logs CPM expression values using plotMDS from the limma package (v3.42.0). Pairwise gene selection using the top 500 genes to show the overall distances between samples. Common gene selection using all 166 detected HALLMARK INFLAMMATORY RESPONSE genes was used to demonstrate the k- means clustering classification.

Differential expression analysis was performed using limma. Samples were assigned into six groups based on the site (T, C or R) and inflammation status (IR or NR) as determined from the k-means clustering of inflammatory genes. The group and sequencing batch were incorporated into the design matrix. Counts were transformed and associated weights calculated using the voomWithQualityWeights function, to also incorporate sample weights. The voomWithQualityWeights output was used with patient as a blocking factor in the duplicatecorrelation function to calculate the intrapatient consensus correlation. The voomWithQualityWeights values were then recalculated with the blocking factor and consensus correlation included and then the output was used in duplicatecorrelation to update the consensus correlation. A linear model was fit using the ImFit function with the final voomWithQualityWeights output, design matrix, blocking factor and consensus correlation. Inflamed and non-inflamed regions within each intestinal site were compared using the contrasts.fit function.

For differential expression analysis, moderated t-statistics were calculated using the treat function with a 1.2-fold cut-off. Differentially expressed genes were determined using a false discovery rate (FDR) adjusted p-value < 0.05. The fry rotational gene set test was used to examine the Caco-2 gene set, incorporating the S149.v.64_8hr log ₂ fold change values as gene weights. Either up- and downregulated genes were used or just upregulated genes. Expression values and associated weights were taken from the voomWithQualityWeights output and the block and consensus correlation values were also used, as with ImFit. Gene set enrichment was indicated using the barcode plot function, with the /-statistics recalculated without a fold-change threshold using eBayes. Gene set testing for different contrasts was performed using the cameraPR function using pre-ranked moderated /-statistics (recalculated without a fold change cut-off using the eBayes function, rather than treat). Relative log ₂ counts per million (CPM) expression values were used for heat maps. The edgeR cpm function was used to obtain logs CPM values. Relative values were obtained for each cell line by subtracting the average logs CPM value for the respective untreated samples, obtained using the cpmByGroup function. Heat map scales were truncated as indicated.

Caco2 RNA Sequencing Analysis scPipe, biomaRt and edgeR were used as above. Lowly expressed genes were instead using the filterByExpr function. A linear model was fit on expression values from voom and the design matrix. Differential expression was again performed with treat and a 1.2-fold threshold. The original multiplexed R1 and R2 FASTQ files were de-multiplexed using cutadapt v3.0 (with error rate 1 and action none) and uploaded to ... with accession number

Metagenomic and RNA Sequencing Analysis

Metagenomic reads were analysed using a reference database for taxonomic classification of metagenomes was constructed using 463 high-quality genome sequences isolated from our PIBD cohort, in addition to the complete RefSeq genomes for Archaea, Viruses and Bacteria, a collection of vectors (NCBI's UniVec db) and the Human genome. Taxonomic assignments were performed with the CCMetagen pipeline v.1.2.5 (Marcelino et al. 2020). Reference sequences were indexed with KMA v.1.3.13 (Clausen et al., 2018) using the '-NI -Sparse TG' options. Mapping was performed with KMA using options '-1t1 -mem mode -and -apm p -ef', and taxonomic assignments were obtained with CCMetagen using options '-du fr -off y', which allowed us to obtain abundance estimates in terms of read counts. Species identified by less than 100 sequence reads were excluded from the analyses, and a Center logratio transformation was applied using the MixOmics R package (Rohart et al, 2017). The inventors next sought to identify bacterial species that most correlate with intestinal inflammation (as identified via RNA-Sequencing analysis) using Sparse Partial Least Squares regression (Cao et al., 2009), and the results were visualized with Clustered Image Maps (MixOmics R package). Bacterial Preparation for Cell Culture

Bacterial isolates were streaked onto YCFA agar plates and incubated for 24 hours in an anaerobic environment. For each isolate of interest, an individual colony was grown overnight in pre-reduced YCFA broth and then twice pelleted by centrifugation and washed with PBS. Following this, the pellet was resuspended in 1 mL of DMEM, and an optical density reading was obtained.

Heat Killed Isolates

For experimentation containing heat killed isolates, the bacteria were prepared as above. Once isolates had been washed twice, they were divided into 1 mL aliquots and heated to 99°C for 60 minutes. Isolates were then plated onto YCFA agar plates and incubated in the anaerobic environment for 24 hours to ensure no viable cells remained.

Correlation of Optical Density with Colony Forming Unit Count

To ensure an accurate multiplicity of infection (MOI) for bacterial stimulations, it was necessary to correlate the optical density (OD) measurements obtained after washing the bacterial pellets, with a colony forming unit count (CFU). Therefore, the bacterial pellets were washed and resuspended as stated above, and then diluted with PBS until an OD ₆₀₀ of 1 was obtained. Following this, they were serially diluted down to 10 ^-6 and plated directly onto YCFA agar. The isolates were allowed to incubate in the anaerobic environment for 24 hours and then enumerated at the dilution factor in which distinct, non-converging colonies were seen. This allowed for a CFU count to be obtained that correlated with an OD ₆ oo of 1 .

Culture and Maintenance of Human Colonic Adenocarcinoma (Caco2) Cells

The Caco2 epithelial cells (ATCC HTB-37) were maintained in complete Dulbecco's Modified Eagle Media (cDMEM), low glucose, GlutaMAX™ Supplement, pyruvate containing 10% v/v fetal calf serum (FCS). Cells were grown in 175cm ² flasks for 48 hours, in 5% CO ₂ at 37°C.

Seeding Caco2 Cells

For bacterial stimulations, cells were seeded into 6-well plates at 1x10 ⁶ cells per well, in a final volume of 2 mL per well of cDMEM. Cells were incubated for 48 hours prior to stimulation. Serum Starving Caco2 Cells

Caco2 cells were serum starved by replacing cDMEM with unmodified DMEM (low glucose, GlutaMAX™ Supplement, pyruvate), 3 hours prior to stimulation of cells with bacterial isolates.

Stimulation of Caco2 Cells with Bacterial Isolates

Bacterial cultures were prepared as described in "Bacterial preparation", and stock cultures for each isolate were created to contain 1x10 ⁷ bacterial cells. The bacterial isolates were then inoculated onto the Caco2 cells at a multiplicity of infection (MOI) of 10:1 , by the addition of 200μl of the bacterial stock solution to the Caco2 cells.

Collection of Cell Supernatant and Cell Lysates

At 0, 2, 4, 6, 8 and 24 hours post infection, 1 mL of the cellular supernatant was collected from each well. The cellular supernatant was then stored at -80°C immediately following collection.

At 0, 2, 4, 6, 8 and 24 hours post infection cellular lysates were collected. 350JJI of RLT lysis buffer, including 1% (v/v) p-mercaptoethanol, was applied to the cells, which were then scraped from the surface of the plate, homogenised via pipette action and immediately stored at -80°C.

Cell cytotoxicity: Lactate dehydrogenase (LDH) Assay

The CytoTox 96® Assay (Promega) was used as per the manufacturer's instructions to determine the LDH release from cells, following stimulation with the bacterial isolates. Additionally, a non-stimulated cell control was used to determine any background cell death, through using cells seeded into a well at the same concentration, without the addition of a bacterial isolate. Finally, a cell culture media control was also used, to account for any impact of the DMEM used.

Colony Forming Unit Counts

For each sample, 50JJI of the cellular supernatant was collected, diluted by a factor of 10 with sterile pre-reduced 1 x PBS, serially diluted down to 10 ^-6 and plated directly onto YCFA agar. To allow for enumeration of the colony forming unit (CFU) counts between samples, 10μl aliquots of each dilution factor were plated in triplicate onto YCFA agar. Plates were enumerated following a 24-hour incubation in the anaerobic environment. Calculations were performed to determine the CFU counts per milliliter of cellular supernatant.

Scanning Electron Microscopy

Images of the bacterial candidates investigated were generated using scanning electron microscopy (SEM). Bacterial isolates were prepared by streaking isolates from previously purified glycerol stocks, stored at -80°C. After 24 hours of incubation in an anaerobic environment, one colony was inoculated into 15 mL of sterile, pre- reduced, YCFA broth and incubated for an additional 24 hours. The culture was then pelleted by centrifugation at 4000 x g for 10 minutes. The supernatant was discarded and the pellet was resuspended in 10 mL of sterile 1 x PBS. The resuspended pellet was re-pelleted by centrifugation at 4000 x g for 10 minutes. This process was repeated twice for each bacterial isolate. Following the second PBS wash, the resuspended pellet was again pelleted by centrifugation at 4000 x g for 10 minutes. The pellet was then resuspended in 1 ml of Karnovsky fixative solution and imaged at Monash Micro Imaging.

EXAMPLE 2 - Identification of Biotherapeutic Strains

A comprehensive bacterial culture collection provides the capacity for cohort specific, reference based metagenomic sequencing and experimental validation (Forster et al. 2019). To build a paediatric inflammatory bowel disease (PIBD) specific culture collection, 286 mucosal biopsies were obtained from 100 paediatric patients (PIBD: 58 patients, control: 42 patients) across three intestinal regions (terminal ileum: 93 samples; caecum: 96 samples; rectum: 97 samples). In total, 6,416 isolates (Bacteroidetes: 3,226 isolates, Firmicutes: 1 ,480 isolates, Actinobacteria: 370 isolates, Proteobacteria: 1 ,321 isolates, Fusobacteria: 17 isolates and Verrucomicrobia: 2 isolates), representing 207 distinct species, including 53 novel species were identified through 16S rRNA capillary sequencing. To provide a cohort specific reference genome database for reference based metagenomic analyses, 463 phylogenetically diverse representative isolates were also subjected to whole genome sequencing.

In addition to accurately defining the microbial community, establishing a detailed understanding of the cellular molecular state at specific tissue sites is essential to fully elucidate the host-microbe interactions. While endoscopy, coupled with histological assessment, represents the gold standard in diagnosis of IBD, these methods remain subjective and lack the molecular resolution that may impact microbial composition and interactions at the mucosal layer. To overcome these limitations, the inventors complemented macroscopic and histological assessment with RNA sequencing based transcriptional profiling on 231 matched samples from the terminal ileum, caecum and rectum of 77 patients.

Initially, the top 500 most variable genes across sample pairs were examined, with the intestinal region exhibiting the greatest influence on gene expression, followed by the samples' histologically defined inflammatory status. However, while the inflammatory state of the sample, defined histologically, is independently related to the variation in gene expression seen across samples, there were a number of histologically inflamed samples clustering with the non-inflamed. Therefore, the inventors sought to define a molecular inflammatory state using k-means consensus clustering, employing genes from the Hallmark inflammatory gene set (Liberzon et al.

2015), to define inflammation. This method identified 69 molecularly inflamed (IBD: 58, non-IBD: 11 ) and 162 molecularly non-inflamed (IBD: 74, non-IBD: 88) samples. Overall, 51 IBD samples (39%) and 10 non-IBD samples (10%) were re-categorised from their histological classification according to their molecular profiles, with classification henceforth based on this molecular state.

Having established a comprehensive, genome sequenced culture collection and accurate classification of molecular inflammatory state within biopsy samples, the inventors next sought to understand the microbial composition within these 231 intestinal samples. While shotgun metagenomic sequencing can provide high resolution measurement at the site of disease, the dominance of human DNA within biopsy samples has limited the application of this best practice approach. To overcome this challenge, the inventors applied a culture based, eukaryotic DNA depletion process, to remove human DNA and allow shotgun metagenomic sequencing on these samples (Figure 1 ). This approach resulted in a median eukaryotic DNA representation of 0.01% of the total sample DNA composition (maximum: 0.03%, minimum: 0%) and provided metagenomic sequencing data suitable for detailed reference based metagenomic analysis.

To understand the host-microbe interactions that underpin phenotypic variations in disease manifestation, the inventors undertook integrated analysis of the metagenomic and transcriptional sequencing in the context of our 463 cohort specific, high quality reference genomes and the RefSeq reference dataset (O'Leary et al.,

2016). This allowed for identification of bacterial clades associated with upregulation of inflammation associated transcriptional pathways, including TNFa signalling via NF-KB (P = 1.6 x 10 ^-22 ), IFNa (P = 2.8 x 10 ^-34 ) and IFNy (P = 2.3 x 10 ^-50 ) response, IL6 JAK STAT3 signalling (P = 1 x 10 ^-39 ), and other Hallmark inflammatory response genes (P = 4.9 x 10 ^-33 ) (Liberzon et al., 2015), while accounting for the compositional nature of the microbiome species abundance data. Notably, known health associated bacteria, including members of the Bacteroides, Parabacteroides and Phocaeicola genera were negatively correlated with genes associated with inflammation, as defined by Hallmark inflammatory signatures, while pathogens and opportunistic pathogens, including members of the Klebsiella and Salmonella genera, and Clostridium perfringens exhibited a positive correlation with inflammation associated Hallmark signatures (Liberzon et al., 2015). In addition to these common pathogens, this analysis also identified an Enterococcus clade positively correlated with inflammatory signalling. Detailed analysis of the Enterococcus isolates cultured from this cohort identified a subclade enriched in IBD patients (P = 0.0077) and a clade with equal representation in both control and IBD patients (Figure 2).

Six representative bacterial isolates with matched genome sequences were selected from the cohort specific, bacterial culture collection (n = 12, 6 IBD-associated clade, 6 control clade) to undertake isolate level genomic analysis and phenotypic characterisation. Consistent with a more severe disease phenotype, genomic assessment of virulence factors in the IBD-associated and control clades identified a higher number of virulence genes within the IBD clade (median: 14 genes) compared to the control clade (median: 11.5 genes, P < 0.01 ; Mann-Whitney U test) (Figure 3). These observations are consistent with previous data in smaller cohorts that predicted Enterococci from IBD patients possess more virulence factors than those in control patients (Golińska et al., 2013). To validate these genomic predictions, cell cytotoxicity, as determined via lactate dehydrogenase (LDH) release into the extracellular medium, was assessed following a 24 hour stimulation of Caco2 intestinal epithelial cells with the bacterial candidates (multiplicity of infection of 10). Contrary to the genomic predictions, isolates from the control clade induced a significantly greater cell cytotoxicity (mean = 42.59%) than isolates within the IBD-associated clade (mean = 11.14%, P = 0.0006; Ratio paired t-test) (Figure 4). Notably, no clade-specific morphological differences were detected by scanning electron microscopy (SEM) (Figures 4 and 5) and cytotoxicity was lost following heat treatment, suggesting the observed phenotype is dependent on bacterial viability (Figure 6).

To further investigate the host-microbe interactions, isolates CC00149 (IBD- associated clade) and CC00064 (control clade) were selected as representative isolates from the subclades identified. RNA sequencing of the transcriptional response to each of these isolates in Caco2 cells was performed at 4, 8 and 24 hours post- stimulation. While the majority of differentially expressed genes were induced in response to both isolates and no differences were observed at 4 hours, 76 isolate specific genes were differentially expressed at 8 hours (57 increased in CC00149, 19 increased in CC00064) (Table 1 ) and 86 genes were differentially expressed at 24 hours (33 increased in CC00149, 53 increased in CC00064) (Table 2; Figure 7). Additionally, the Hallmark hypoxic response was most significantly upregulated in response to stimulation with CC00064 (P = 1.41 x 1O ^-10 ) and the Hallmark TNFa signalling via NF-KB response was most significantly upregulated in response to CC00149 (P= 7.9 x 10 ^-15 ) at 8 hours post-stimulation (Liberzon et al., 2015) (Table 3).

To understand the relationship between clade-specific transcriptional changes and cell cytotoxicity observed at 24 hours, the inventors focused on the 76 genes that were either significantly upregulated or downregulated in CC00149 relative to CC00064 at 8 hours post-stimulation. Differences at this time point could represent key responses without confounding factors associated with extensive cell cytotoxicity at 24 hours. Consistent with this hypothesis, gene set testing identified TNFa signalling via NF-KB (P = 7.3 x 10 ^-16 ), activation of pro-apoptotic pathways (P= 2.91 x 10 ^-2 ), and initiation of the unfolded protein response (P = 2.42 x 10 ^-7 ) to be significantly upregulated in response to CC00149, compared to CC00064 (Table 4).

To assess the clinical relevance of these key clade-specific responses, the inventors next compared the transcriptional profile measured in our Caco2 in vitro model to those observed in the mucosal samples (n = 231 ) from our patient cohort (n = 77). Focusing on the 76 genes that were significantly differentially expressed in the in vitro Caco2 cells at 8 hours, within the patient cohort dataset, a significant enrichment was observed in patient biopsy samples derived from both the caecum (P = 3.6 x 10 ^-8 ) and rectum (P = 8.6 x 10 ^-9 ). However, no such relationship was detected in the terminal ileum (P = 0.82) (Figure 8), which may be explained by the colonic origin of the Caco2 cells in our co-culture model. In total, 34.2% (n = 26) of the 76 genes were statistically differentially expressed and exhibited the same direction of expression change (20 upregulated, 6 downregulated) in both the Caco2 model system and the primary biopsy samples (Figure 8). Importantly, these included genes known to be associated with cell death and the unfolded protein response including CHAC1 (P = 1.53 x 10 ^-8 ), CEBPB (P = 3.37 x 10 ^-7 ), TRIB3 (P = 5.25 x 10 ^-6 ), PPP1R15A (P = 0.011 ), DDIT3 (P = 4.5 x 10 ^-5 ), ATF4 (P = 0.020) and XBP1 (P = 0.043). Taken together, these results suggest a clade-specific differential regulation of cell death pathways associated with IBD mucosal immune state that may be suitable for clinical intervention in selected patients. In total, 44 new strains of a new clade, referred herein to clade 149, of non- inflammatory Enterococcus were identified represented by the 16s rRNA gene sequences provided as SEQ ID NO's 1 to 44, and including Enterococcus sp. CC00149 deposited under V19/018754, and Enterococcus sp. CC00259 deposited under V19/018755, on 9 September 2019 at the National Measurement Institute, Australia, as well as Enterococcus sp. CC00620 deposited under V21/013048 on 29 June 2021 at the National Measurement Institute, Australia. Furthermore, 30 new strains of a new clade, referred herein to clade 64, of inflammatory Enterococcus, closely related to the above-mentioned non-inflammatory Enterococcus, were identified represented by the 16s rRNA gene sequences provided as SEQ ID NO's 45 to 74, and including Enterococcus sp. CC00064 deposited under V21/013046, and Enterococcus sp. CC00619 deposited under V21/013047, on 29 June 2021 at the National Measurement Institute, Australia, as well as Enterococcus sp. CC00262 deposited under V20/006238 on 18 March 2020 at the National Measurement Institute, Australia and Enterococcus sp. CC0002 deposited under V21/014119 on 20 July 2021 at the National Measurement Institute, Australia.

Table 1. Differentially expressed genes at 8 hours.

Table 2. Differentially expressed genes at 24 hours.

Table 3. Hallmark responses.

Table 4. Clade-specific transcriptional changes and cell cytotoxicity observed at 24 hours.

EXAMPLE 3 - Detection of Biotherapeutic Strains

Detection and differentiation of bacterial strains of the invention provides the capacity to target beneficial therapeutic interventions, both conventional and microbiome based, to best suit patient needs. This capacity has the potential for use as a companion diagnostic for stratification of, for example, IBD patients. Multiple methods could be applied to achieve this differentiation including DNA, RNA, metabolic signature and protein-based assays. The following data demonstrates the implementation of this method for specific detection using a DNA and qPCR-based implementation.

Methods qPCR was performed on a QuantStudio™ 6 Flex Real-Time PCR System using the following primers:

Non-lnflammatory (non-cytotoxic) strains

Forward: CGAGGTACACCGTAAGTTGTTGCT (SEQ ID NO: 75)

Reverse: ACCAGGACACGTAGACTTCACAGT (SEQ ID NO: 76)

Inflammatory (cytotoxic) strains

Forward: CCTTTACCATGGCCGTGTAGC (SEQ ID NO: 83)

Reverse: CCATGAATGGTGTTCCTCCTTCTC (SEQ ID NO: 84)

The DNA was diluted 1 :5 in DEPC-treated water (Applied Biosystems). Applied Biosystems SYBR Magic Master Mix (Applied Biosystems; Foster City, California, USA) was used in total reaction volumes of 10μl. Each reaction contained 5μl of the SYBR Magic Master Mix, 0.2μl each of the appropriate forward and reverse primers (final concentration 0.2mM each), 2μl of DNA, and 2.6μl of DEPC water. The samples were loaded in triplicate into MicroAmp Optical 384-well Reaction plates (Applied Biosystems), sealed with a MicroAmp Optical adhesive cover. A no-template negative control was included on all plates.

Both primer sets were found to be clade specific, distinguishing between noninflammatory strains and inflammatory strains of the invention (Figure 9). EXAMPLE 4 - Comparison of Sequence Identities

The strain of the invention, comprising a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 74 (and, in particular, SEQ ID NO 45), was compared to SEQ ID NO's 89 to 217 using sequence identity analysis.

In a preferred embodiment of the invention, the strain of the invention comprising a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 74 does not comprise any one of SEQ ID NO's 89 to 217 presented in Table 5. As discussed above under the preferred embodiments, in a further embodiment, the strain of, or for use in, the invention comprises a 16s ribosomal RNA (rRNA) gene having a nucleotide sequence as shown in any one of SEQ ID NO's 1 to 74, or a nucleotide sequence at least 90%, at least 90.5%, at least 91%, at least 91.5%, at least 92%, at least 92.5%, at least 93%, at least 93.5%, at least 94%, at least 94.5%, at least 95%, at least 95.5%, at least 96%, at least 96.5%, at least 97%, at least 97.5%, at least 98%, at least 98.5%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to one or more of SEQ ID NO's 1 to 74, and wherein the 16s ribosomal RNA (rRNA) gene does not comprise any one of SEQ ID NO's 89 to 217. In an alternative embodiment, the 16s ribosomal RNA (rRNA) gene does comprise any one of SEQ ID NO's 89 to 217.

It will be appreciated by persons skilled in the art that numerous variations and/or modifications may be made to the invention as shown in the specific embodiments without departing from the spirit or scope of the invention as broadly described. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive.

All publications discussed and/or referenced herein are incorporated herein in their entirety.

Any discussion of documents, acts, materials, devices, articles or the like which has been included in the present specification is solely for the purpose of providing a context for the present invention. It is not to be taken as an admission that any or all of these matters form part of the prior art base or were common general knowledge in the field relevant to the present invention as it existed before the priority date of each claim of this application. REFERENCES

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Graham and Xavier (2020) Nature 578:527-539.

Forster et al. (2019) Nat. Biotechnol 37:186-192.

Liberzon et al. (2015) Cell Syst. 1 :417-425.

Liu et al (2019) Nucleic Acids Res. 47(D1 ):D687-D692

Marcelino et al. (2020) Genome Biology 21 :103.

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NUCLEOTIDE SEQUENCES

Table 5: Patentin Field Identifier and Field Name Key Sequences:

The key presented above in Table 5 is used to interpret the information presented below:

<110> Hudson Institute of Medical Research

<120> BIOTHERAPEUTIC ENTEROCOCCUS ISOLATES

<130> 295050

<160> 217

<170> Patentin version 3.5

<210> 1

<211 > 1179

<212> DNA

<213> Enterococcus sp. isolate CC00149

<400> 1 ctatacatgc agtcgtacgc ttctttttcc accggagctt gctccaccgg aaaaagaaga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggccacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 900 ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960 ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa gtgacaggtg 1020 gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca 1080 acccttattg ttagttgcca tcattcagtt gggcactcta gcaagactgc cggtgacaaa 1140 ccggaggaag gtggggatga cgtcaaatca tcatgcccc 1179 <210> 2

<211> 1159

<212> DNA

<213> Enterococcus sp. isolate CC00259

<400> 2 ctatacatgc agtcgtacgc ttctttttcc accggagctt gctccaccgg aaaaagaaga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggccacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 900 ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960 ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa gtgacaggtg 1020 gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca 1080 acccttattg ttagttgcca tcattcagtt gggcactcta gcaagactgc cggtgacaaa 1140 ccggaggaag gtggggatg 1159

<210> 3

<211> 1214

<212> DNA

<213> Enterococcus sp. isolate CC00261

<400> 3 atacatgcag tcgtacgctt ctttttccac cggagcttgc tccaccggaa aaagaagagt 60 ggcgaacggg tgagtaacac gtgggtaacc tgcccatcag aaggggataa cacttggaaa 120 caggtgctaa taccgtataa caatcgaaac cgcatggttt tgatttgaaa ggcgctttcg 180 ggtgtcgctg atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca 240 aggccacgat gcatagccga cctgagaggg tgatcggcca cattgggact gagacacggc 300 ccaaactcct acgggaggca gcagtaggga atcttcggca atggacgaaa gtctgaccga 360 gcaacgccgc gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac 420 aaggatgaga gtaactgttc atcccttgac ggtatctaac cagaaagcca cggctaacta 480 cgtgccagca gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa 540 agcgagcgca ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt 600 cattggaaac tgggagactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg 660 aaatgcgtag atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga 720 cgctgaggct cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt 780 aaacgatgag tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta 840 agcactccgc ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc 900 gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc gaagaacctt accaggtctt 960 gacatccttt gaccactcta gagatagagc ttccccttcg ggggcaaagt gacaggtggt 1020 gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac 1080 ccttattgtt agttgccatc attcagttgg gcactctagc aagactgccg gtgacaaacc 1140 ggaggaaggt ggggatgacg tcaaatcatc atgcccctta tgaccggggc tacccacgtg 1200 ctacaatggg aagt 1214

<210> 4

<211> 1192

<212> DNA

<213> Enterococcus sp. isolate CC00260

<400> 4 ctatacatgc agtcgtacgc ttctttttcc accggagctt gctccaccgg aaaaagaaga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggccacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 900 ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960 ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa gtgacaggtg 1020 gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca 1080 acccttattg ttagttgcca tcattcagtt gggcactcta gcaagactgc cggtgacaaa 1140 ccggaggaag gtggggatga cgtcaaatca tcatgcccct tatgaccggg gc 1192

<210> 5

<211 > 1179

<212> DNA

<213> Enterococcus sp. isolate CC00620

<400> 5 acatgcagtc gtacgcttgc tattttccac cggagcttgc tccaccggaa aaagaagagt 60 ggcgaacggg tgagtaacac gtgggtaacc tgcccatcag aaggggataa cacttggaaa 120 caggtgctaa taccgtataa caatcgaaac cgcatggttt tgatttgaaa ggcgctttcg 180 ggtgtcgctg atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca 240 aggccacgat gcatagccga cctgagaggg tgatcggcca cattgggact gagacacggc 300 ccaaactcct acgggaggca gcagtaggga atcttcggca atggacgaaa gtctgaccga 360 gcaacgccgc gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac 420 aaggatgaga gtaactgttc atcccttgac ggtatctaac cagaaagcca cggctaacta 480 cgtgccagca gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa 540 agcgagcgca ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt 600 cattggaaac tgggagactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg 660 aaatgcgtag atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga 720 cgctgaggct cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt 780 aaacgatgag tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta 840 agcactccgc ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc 900 gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc gaagaacctt accaggtctt 960 gacatccttt gaccactcta gagatagagc ttccccttcg ggggcaaagt gacaggtggt 1020 gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac 1080 ccttattgtt agttgccatc attcagttgg gcactctagc aagactgccg gtgacaaacc 1140 ggaggaaggg gggggatgac ggccagatca tcatgcgcc 1179 <210> 6

<211> 1186

<212> DNA

<213> Enterococcus sp. isolate CC00152

<400> 6 tgcagtcgta cgcttctttt tccaccggag cttgctccac cggaaaaaga agagtggcga 60 acgggtgagt aacacgtggg taacctgccc atcagaaggg gataacactt ggaaacaggt 120 gctaataccg tataacaatc gaaaccgcat ggttttgatt tgaaaggcgc tttcgggtgt 180 cgctgatgga tggacccgcg gtgcattagc tagttggtga ggtaacggct caccaaggcc 240 acgatgcata gccgacctga gagggtgatc ggccacattg ggactgagac acggcccaaa 300 ctcctacggg aggcagcagt agggaatctt cggcaatgga cgaaagtctg accgagcaac 360 gccgcgtgag tgaagaaggt tttcggatcg taaaactctg ttgttagaga agaacaagga 420 tgagagtaac tgttcatccc ttgacggtat ctaaccagaa agccacggct aactacgtgc 480 cagcagccgc ggtaatacgt aggtggcaag cgttgtccgg atttattggg cgtaaagcga 540 gcgcaggcgg tttcttaagt ctgatgtgaa agcccccggc tcaaccgggg agggtcattg 600 gaaactggga gacttgagtg cagaagagga gagtggaatt ccatgtgtag cggtgaaatg 660 cgtagatata tggaggaaca ccagtggcga aggcggctct ctggtctgta actgacgctg 720 aggctcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac gccgtaaacg 780 atgagtgcta agtgttggag ggtttccgcc cttcagtgct gcagctaacg cattaagcac 840 tccgcctggg gagtacgacc gcaaggttga aactcaaagg aattgacggg ggcccgcaca 900 agcggtggag catgtggttt aattcgaagc aacgcgaaga accttaccag gtcttgacat 960 cctttgacca ctctagagat agagcttccc cttcgggggc aaagtgacag gtggtgcatg 1020 gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccctta 1080 ttgttagttg ccatcattca gttgggcact ctagcaagac tgccggtgac aaaccggagg 1140 aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc ggggct 1186

<210> 7

<211> 1112

<212> DNA

<213> Enterococcus sp. isolate mc056tix_3d_mc056_57_d3

<400> 7 ctatacatgc agtcgaacgc ttctttttcc accggagctt gctccaccgg aaaaagagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggcgacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgaag ctcgaaagcg tgggggagca aacaggatta gataccctgg gtagtccacg 780 cccgtaaacg atgaagtgct aagtgttgga agggtttccg ccccttcagt gctgcaggct 840 aacgcattta agcactccgc cctgggggag tacgaaccgc caaggttgaa aactcaaaag 900 gaaattgacc ggggggcccg caccaagcgg gtggaagcat gtgggtttaa tttcgaagcc 960 aacgccgaag aaacctttac cagggtcttg gacatccctt ttgaaccact cctagaagat 1020 aggaagcttc cccccttccg gggggcaaaa gtgaacaagg tgggtggcat gggtttgtcc 1080 gtccagcctc cgtgtccctg gagaatgttt gg 1112

<210> 8

<211 > 1102

<212> DNA

<213> Enterococcus sp. isolate mc022rnx_e1

<400> 8 atacatgcag tcgaacgctt ctttttccac cggagcttgc tccaccggaa aaagaggagt 60 ggcgaacggg tgagtaacac gtgggtaacc tgcccatcag aaggggataa cacttggaaa 120 caggtgctaa taccgtataa caatcgaaac cgcatggttt tgatttgaaa ggcgctttcg 180 ggtgtcgctg atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca 240 aggcgacgat gcatagccga cctgagaggg tgatcggcca cattgggact gagacacggc 300 ccaaactcct acgggaggca gcagtaggga atcttcggca atggacgaaa gtctgaccga 360 gcaacgccgc gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac 420 aaggatgaga gtaactgttc atcccttgac ggtatctaac cagaaagcca cggctaacta 480 cgtgccagca gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa 540 agcgagcgca ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt 600 cattggaaac tgggagactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg 660 aaatgcgtag atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga 720 cgctgaggct cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt 780 aaacgatgag tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta 840 agcactccgc ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc 900 gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc gaagaacctt accaggtctt 960 gacatccttt gaccactcta gagatagagc ttccccttcg ggggcaaagt gacaggtggt 1020 gcatggttgt cgtcagctcg tgtcgtgaga tgttggggtt aagtcccgca acgaagcgca 1080 acccttattg gttagttgcc at 1102

<210> 9

<211 > 1178

<212> DNA

<213> Enterococcus sp. isolate mc056tix_3h_mc056_57_h3

<400> 9 ctatacatgc agtcgaacgc ttctttttcc accggagctt gctccaccgg aaaaagagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggcgacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 900 ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960 ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa gtgacaggtg 1020 gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca 1080 acccttattg ttagttgcca tcatttagtt gggcactcta gcaagactgc cggtgacaaa 1140 ccggaggaag gtggggatga cgtcaaatca tcatggcc 1178

<210> 10

<211 > 1082

<212> DNA <213> Enterococcus sp. isolate mc056tix_3f_mc056_57_f3

<400> 10 ctatacatgc agtcgaacgc ttctttttcc accggagctt gctccaccgg aaaaagagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggcgacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggg 900 cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaaa ccttaccagg 960 tcttgacatc ctttgaccac tctagagata gagcttcccc ttcgggggca aagtgacaag 1020 tggttgcatg ggttgtcgtc agcctcgtgt cctgaaaatg ttggggttaa gtccccgcaa 1080 eg 1082

<210> 11

<211 > 988

<212> DNA

<213> Enterococcus sp. isolate mc036cna_f03

<220>

<221> misc_feature

<222> (907).. (907)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (923).. (923)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (928).. (928)

<223> n is a, c, g, or t <220>

<221> misc_feature

<222> (936).. (936)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (939).. (939)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (947).. (949)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (965).. (965)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (967).. (967)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (975).. (975)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (979).. (979)

<223> n is a, c, g, or t

<400> 11 agtcgaacgc ttctttttcc accggagctt gctccaccgg aaaaagagga gtggcgaacg 60 ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga aacaggtgct 120 aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt cgggtgtcgc 180 tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac caaggcgacg 240 atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg gcccaaactc 300 ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc gagcaacgcc 360 gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga acaaggatga 420 gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac tacgtgccag 480 cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt aaagcgagcg 540 caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg gtcattggaa 600 actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg tgaaatgcgt 660 agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact gacgctgagg 720 ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc gtaaacgatg 780 agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat taagcactcc 840 gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc 900 ggtggancat gtggtttaat tcnaagcnac gcgaanaanc cttaccnnnc ttgacatcct 960 ttgancnctc taganatana gcttcccc 988

<210> 12

<211> 1184

<212> DNA

<213> Enterococcus sp. isolate mc056tix_4a_mc056_57_a4

<400> 12 ggcgtgctat acatgcagtc gaacgcttct ttttccaccg gagcttgctc caccggaaaa 60 agaggagtgg cgaacgggtg agtaacacgt gggtaacctg cccatcagaa ggggataaca 120 cttggaaaca ggtgctaata ccgtataaca atcgaaaccg catggttttg atttgaaagg 180 cgctttcggg tgtcgctgat ggatggaccc gcggtgcatt agctagttgg tgaggtaacg 240 gctcaccaag gcgacgatgc atagccgacc tgagagggtg atcggccaca ttgggactga 300 gacacggccc aaactcctac gggaggcagc agtagggaat cttcggcaat ggacgaaagt 360 ctgaccgagc aacgccgcgt gagtgaagaa ggttttcgga tcgtaaaact ctgttgttag 420 agaagaacaa ggatgagagt aactgttcat cccttgacgg tatctaacca gaaagccacg 480 gctaactacg tgccagcagc cgcggtaata cgtaggtggc aagcgttgtc cggatttatt 540 gggcgtaaag cgagcgcagg cggtttctta agtctgatgt gaaagccccc ggctcaaccg 600 gggagggtca ttggaaactg ggagacttga gtgcagaaga ggagagtgga attccatgtg 660 tagcggtgaa atgcgtagat atatggagga acaccagtgg cgaaggcggc tctctggtct 720 gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag gattagatac cctggtagtc 780 cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc gcccttcagt gctgcagcta 840 acgcattaag cactccgcct ggggagtacg accgcaaggt tgaaactcaa aggaattgac 900 gggggcccgc acaagcggtg gagcatgtgg tttaattcga agcaacgcga agaaccttac 960 caggtcttga catcctttga ccactctaga gatagagctt ccccttcggg ggcaaagtga 1020 caggtggtgc atggttgtcg tcagctcgtg tcatgagatg ttgggttaag tcccgcacga 1080 gcgcaaccct tattgttagt tgccatcatt tagttgggca ctctagcagg actgccggtg 1140 acaaaccgga ggaagttggg gatgacgtca aatcatcatg cccc 1184

<210> 13

<211> 1119

<212> DNA

<213> Enterococcus sp. isolate mc056tix_3e_mc056_57_e3

<400> 13 ctatacatgc agtcgaacgc ttctttttcc accggagctt gctccaccgg aaaaagagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggcgacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 900 ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960 ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa gtgacaggtg 1020 ggtgcagggt tgtccgtcac ctcctgtcct gagatgttgg ggttaagtcc cggcaacgaa 1080 ccgcaaccct tattggttag ttgccatcat tttagttgg 1119

<210> 14

<211> 1191

<212> DNA

<213> Enterococcus sp. isolate mc056cix_8d_mc056_57_d8

<400> 14 ctatacatgc agtcgaacgc ttctttttcc accggagctt gctccaccgg aaaaagagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggcgacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtacacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 900 ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960 ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa gtgacaggtg 1020 gtgcatggtt gtcgtcagct cgtgtcctga gatgttgggt taagtcccgc aacgagcgca 1080 acccttattg ttagttgcca tcatttagtt gggcactcta gcaagactgc cggtgacaaa 1140 ccggaggaag gtggggatga cgtcaaatca tcatgcccct tatgaactgg g 1191

<210> 15

<211 > 1208

<212> DNA

<213> Enterococcus sp. isolate mc056cix_8e_mc056_57_e8

<400> 15 ctatacatgc agtcgaacgc ttctttttcc accggagctt gctccaccgg aaaaagagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggcgacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 900 ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960 ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa gtgacaggtg 1020 gtgcatggtt gtcgtcagct cgtgtcctga gatgttgggt taagtcccgc aacaagcgca 1080 acccttattg ttagttgcca tcatttagtt gggcactcta gcaggactgc ccgtgaaaaa 1140 accggaggaa gggtggggat gaacgtcaaa tcatcatgcc cccttatgaa ctggggctaa 1200 caacctgg 1208

<210> 16

<211> 1157

<212> DNA

<213> Enterococcus sp. isolate mc056tix_3a_mc056_57_a3

<400> 16 ctatacatgc agtcgaacgc ttctttttcc accggagctt gctccaccgg aaaaagagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggcgacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 900 ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960 ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa gtgacaggtg 1020 gtgcagggtt gtcgtcagct cgtgtcgtga aatgttgggt taagtcccgc aacaagcgca 1080 acccttatgg ttagttgcca tcatttagtt gggcattcta gcaagactgc ccggtgacaa 1140 accggaagga aggtggg 1157

<210> 17

<211> 1129

<212> DNA

<213> Enterococcus sp. isolate mc056tix_1c_mc056_57_c1

<400> 17 ctatacatgc agtcgaacgc ttctttttcc accggagctt gctccaccgg aaaaagagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggcgacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggga gtacgaccgc aaggttgaaa ctcaaaggaa ttgacggggg 900 gcccgcacaa ccggtggagc atgtggttta attcgaagca acgcgaagaa accttaccag 960 ggtcttgaca tccctttgac cactctagag atagaagctt cccccttcgg gggcaaagtg 1020 accaggtggt tgcatgggtt gtcgtcaacc tcgtgtccgt gaaaatgttg gggttaaatt 1080 cccgcaaaca agcgcaaacc tttattggtt aattggccat ccatttaat 1129

<210> 18

<211 > 1167

<212> DNA

<213> Enterococcus sp. isolate mc056tix_3b_mc056_57_b3

<400> 18 ctatacatgc agtcgaacgc ttctttttcc accggagctt gctccaccgg aaaaagagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggcgacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggg 900 cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt 960 cttgacatcc tttgaccact ctagagatag agcttccccc ttcgggggca aagtgacaag 1020 ggggtgcatg gtttgtcgtc agcttcgtgt cctggagatg ttggggttaa gtcccgcaac 1080 caagcgccac ccctaattgt taatttgcca tcattttagt tggggcactt ctagccagga 1140 ctggccgggt gacaaaaccg gaaggaa 1167

<210> 19

<211 > 998

<212> DNA

<213> Enterococcus sp. isolate mc036rna2_h03

<220>

<221> misc_feature

<222> (908).. (908)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (937).. (937)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (966).. (966)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (974).. (974)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (976).. (976)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (980).. (980)

<223> n is a, c, g, or t

<400> 19 aagtcgaacg cttctttttc caccggagct tgctccaccg gaaaaagagg agtggcgaac 60 gggtgagtaa cacgtgggta acctgcccat cagaagggga taacacttgg aaacaggtgc 120 taataccgta taacaatcga aaccgcatgg ttttgatttg aaaggcgctt tcgggtgtcg 180 ctgatggatg gacccgcggt gcattagcta gttggtgagg taacggctca ccaaggcgac 240 gatgcatagc cgacctgaga gggtgatcgg ccacattggg actgagacac ggcccaaact 300 cctacgggag gcagcagtag ggaatcttcg gcaatggacg aaagtctgac cgagcaacgc 360 cgcgtgagtg aagaaggttt tcggatcgta aaactctgtt gttagagaag aacaaggatg 420 agagtaactg ttcatccctt gacggtatct aaccagaaag ccacggctaa ctacgtgcca 480 gcagccgcgg taatacgtag gtggcaagcg ttgtccggat ttattgggcg taaagcgagc 540 gcaggcggtt tcttaagtct gatgtgaaag cccccggctc aaccggggag ggtcattgga 600 aactgggaga cttgagtgca gaagaggaga gtggaattcc atgtgtagcg gtgaaatgcg 660 tagatatatg gaggaacacc agtggcgaag gcggctctct ggtctgtaac tgacgctgag 720 gctcgaaagc gtggggagca aacaggatta gataccctgg tagtccacgc cgtaaacgat 780 gagtgctaag tgttggaggg tttccgccct tcagtgctgc agctaacgca ttaagcactc 840 cgcctgggga gtacgaccgc aaggttgaaa ctcaaaggaa ttgacggggg cccgcacaag 900 cggtgganca tgtggtttaa ttcgaagcaa cgcgaanaac cttaccaggt cttgacatcc 960 tttgancact ctananatan agcttcccct tcgggggc 998

<210> 20

<211 > 1163

<212> DNA

<213> Enterococcus sp. isolate mc056cix_5f_mc056_57_f5

<400> 20 ctatacatgc agtcgaacgc ttctttttcc accggagctt gctccaccgg aaaaagagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggcgacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 900 ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960 ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa gtgacaggtg 1020 gtgcatggtt gtcgtcagct cgtgtcctga aatgttgggt taagtcccgc aacgagcgca 1080 acccttattg ttagttgcca tcatttaagt ggggcactct agcaagactg cccggtgaca 1140 aaccggaagg aaggtggggg atg 1163

<210> 21

<211 > 1204

<212> DNA

<213> Enterococcus sp. isolate mc056cix_6d_mc056_57_d6

<400> 21 ctatacatgc agtcgaacgc ttctttttcc accggagctt gctccaccgg aaaaagagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggcgacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 900 ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960 ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa gtgacaggtg 1020 gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca 1080 acccttattg ttagttgcca tcatttagtt ggggactcta gcaagactgc cggtggacaa 1140 accggaggaa ggtggggatg acgtcaaatc atcatgcccc ttatgacctg ggctaccacc 1200 gtgc 1204

<210> 22 <211> 1183

<212> DNA

<213> Enterococcus sp. isolate mc056tix_3g_mc056_57_g3

<400> 22 tgcagtcgaa cgcttctttt tccaccggag cttgctccac cggaaaaaga ggagtggcga 60 acgggtgagt aacacgtggg taacctgccc atcagaaggg gataacactt ggaaacaggt 120 gctaataccg tataacaatc gaaaccgcat ggttttgatt tgaaaggcgc tttcgggtgt 180 cgctgatgga tggacccgcg gtgcattagc tagttggtga ggtaacggct caccaaggcg 240 acgatgcata gccgacctga gagggtgatc ggccacattg ggactgagac acggcccaaa 300 ctcctacggg aggcagcagt agggaatctt cggcaatgga cgaaagtctg accgagcaac 360 gccgcgtgag tgaagaaggt tttcggatcg taaaactctg ttgttagaga agaacaagga 420 tgagagtaac tgttcatccc ttgacggtat ctaaccagaa agccacggct aactacgtgc 480 cagcagccgc ggtaatacgt aggtggcaag cgttgtccgg atttattggg cgtaaagcga 540 gcgcaggcgg tttcttaagt ctgatgtgaa agcccccggc tcaaccgggg agggtcattg 600 gaaactggga gacttgagtg cagaagagga gagtggaatt ccatgtgtag cggtgaaatg 660 cgtagatata tggaggaaca ccagtggcga aggcggctct ctggtctgta actgacgctg 720 aggctcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac gccgtaaacg 780 atgagtgcta agtgttggag ggtttccgcc cttcagtgct gcagctaacg cattaagcac 840 tccgcctggg gagtacgacc gcaaggttga aactcaaagg aattgacggg ggcccgcaca 900 agcggtggag catgtggttt aattcgaagc aacgcgaaga accttaccag gtcttgacat 960 cctttgacca ctctagagat agagcttccc cttcgggggc aaagtgacag gtggtgcatg 1020 gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccctta 1080 ttgttagttg ccatcattta gttgggcact ctagcaagac tgccggtgac aaaccggagg 1140 aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc tgg 1183

<210> 23

<211 > 1175

<212> DNA

<213> Enterococcus sp. isolate mc056cix_8c_mc056_57_c8

<400> 23 acatgcagtc gaacgcttct ttttccaccg gagcttgctc caccggaaaa agaggagtgg 60 cgaacgggtg agtaacacgt gggtaacctg cccatcagaa ggggataaca cttggaaaca 120 ggtgctaata ccgtataaca atcgaaaccg catggttttg atttgaaagg cgctttcggg 180 tgtcgctgat ggatggaccc gcggtgcatt agctagttgg tgaggtaacg gctcaccaag 240 gcgacgatgc atagccgacc tgagagggtg atcggccaca ttgggactga gacacggccc 300 aaactcctac gggaggcagc agtagggaat cttcggcaat ggacgaaagt ctgaccgagc 360 aacgccgcgt gagtgaagaa ggttttcgga tcgtaaaact ctgttgttag agaagaacaa 420 ggatgagagt aactgttcat cccttgacgg tatctaacca gaaagccacg gctaactacg 480 tgccagcagc cgcggtaata cgtaggtggc aagcgttgtc cggatttatt gggcgtaaag 540 cgagcgcagg cggtttctta agtctgatgt gaaagccccc ggctcaaccg gggagggtca 600 ttggaaactg ggagacttga gtgcagaaga ggagagtgga attccatgtg tagcggtgaa 660 atgcgtagat atatggagga acaccagtgg cgaaggcggc tctctggtct gtaactgacg 720 ctgaggctcg aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa 780 acgatgagtg ctaagtgttg gagggtttcc gcccttcagt gctgcagcta acgcattaag 840 cactccgcct ggggagtacg accgcaaggt tgaaactcaa aggaattgac gggggcccgc 900 acaagcggtg gagcatgtgg tttaattcga agcaacgcga agaaccttac caggtcttga 960 catcctttga ccactctaga gatagagctt ccccttcggg ggcaaagtga caggtggtgc 1020 atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg agcgcaaccc 1080 ttattgttag ttgccatcat ttagttgggc actctagcaa gactgccggt gacaaaccgg 1140 aggaaggtgg gggatgacgt caaatcatca tgccc 1175

<210> 24

<211 > 938

<212> DNA

<213> Enterococcus sp. isolate mc056cix_7h_mc056_57_h7

<400> 24 ctatacatgc agtcgaacgc ttctttttcc accggagctt gctccaccgg aaaaagagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggcgacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaggaatt gacgggggcc 900 cgcacagcgg tggagcatgt ggtttattcg aagcaacg 938

<210> 25

<211> 1194

<212> DNA

<213> Enterococcus sp. isolate mc056tix_2b_mc056_57_b2

<400> 25 gcgtgctata catgcagtcg aacgcttctt tttccaccgg agcttgctcc accggaaaaa 60 gaggagtggc gaacgggtga gtaacacgtg ggtaacctgc ccatcagaag gggataacac 120 ttggaaacag gtgctaatac cgtataacaa tcgaaaccgc atggttttga tttgaaaggc 180 gctttcgggt gtcgctgatg gatggacccg cggtgcatta gctagttggt gaggtaacgg 240 ctcaccaagg cgacgatgca tagccgacct gagagggtga tcggccacat tgggactgag 300 acacggccca aactcctacg ggaggcagca gtagggaatc ttcggcaatg gacgaaagtc 360 tgaccgagca acgccgcgtg agtgaagaag gttttcggat cgtaaaactc tgttgttaga 420 gaagaacaag gatgagagta actgttcatc ccttgacggt atctaaccag aaagccacgg 480 ctaactacgt gccagcagcc gcggtaatac gtaggtggca agcgttgtcc ggatttattg 540 ggcgtaaagc gagcgcaggc ggtttcttaa gtctgatgtg aaagcccccg gctcaaccgg 600 ggagggtcat tggaaactgg gagacttgag tgcagaagag gagagtggaa ttccatgtgt 660 agcggtgaaa tgcgtagata tatggaggaa caccagtggc gaaggcggct ctctggtctg 720 taactgacgc tgaggctcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc 780 acgccgtaaa cgatgagtgc taagtgttgg agggtttccg cccttcagtg ctgcagctaa 840 cgcattaagc actccgcctg gggagtacga ccgcaaggtt gaaactcaaa ggaattgacg 900 ggggcccgca caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc 960 aggtcttgac atcctttgac cactctagag atagagcttc cccttcgggg gcaaagtgac 1020 aggtggtgca tggttgtcgt cagctcgtgt cgtgaaatgt tgggttaagt cccgcaacga 1080 gcgcaaccct tattgttagt tgccatcatt tagttgggca ctctagcaag actgccggtg 1140 acaaaccgga ggaaggtggg gatgacgtca aatcatcatg ccccttatga ctgg 1194

<210> 26

<211> 1137

<212> DNA

<213> Enterococcus sp. isolate mc017tn_x_b6

<400> 26 tatacatgca gtcgtacgct tgctttttct caccggagct tgctccaccg gaaaaagaag 60 agtggcgaac gggtgagtaa cacgtgggta acctgcccat cagaagggga taacacttgg 120 aaacaggtgc taataccgta taacaatcga aaccgcatgg ttttg atttg aaaggcgctt 180 tcgggtgtcg ctgatggatg gacccgcggt gcattagcta gttggtgagg taacggctca 240 ccaaggccac gatgcatagc cgacctgaga gggtgatcgg ccacattggg actgagacac 300 ggcccaaact cctacgggag gcagcagtag ggaatcttcg gcaatggacg aaagtctgac 360 cgagcaacgc cgcgtgagtg aagaaggttt tcggatcgta aaactctgtt gttagagaag 420 aacaaggatg agagtaactg ttcatccctt gacggtatct aaccagaaag ccacggctaa 480 ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg ttgtccggat ttattgggcg 540 taaagcgagc gcaggcggtt tcttaagtct gatgtgaaag cccccggctc aaccggggag 600 ggtcattgga aactgggaga cttgagtgca gaagaggaga gtggaattcc atgtgtagcg 660 gtgaaatgcg tagatatatg gaggaacacc agtggcgaag gcggctctct ggtctgtaac 720 tgacgctgag gctcgaaagc gtggggagca aacaggatta gataccctgg tagtccacgc 780 cgtaaacgat gagtgctaag tgttggaggg tttccgccct tcagtgctgc agctaacgca 840 ttaagcactc cgcctgggga gtacgaccgc aaggttgaaa ctcaaaggaa ttgacggggg 900 cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt 960 cttgacatcc tttgaccact ctagagatag agcttcccct tcgggggcaa agtgacaggt 1020 ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg gttaagtccc cgcaacgaag 1080 cgcaaccctt tattgttagt tgccatcatt tcattggggc actctagcaa gactggc 1137

<210> 27

<211> 1087

<212> DNA

<213> Enterococcus sp. isolate mc017cn_x_d3

<400> 27 ctataatgca agtcgtacgc tgtgctattt gctaagcgga gcttgctcca ccggaaaaag 60 aagagtggcg aacgggtgag taacacgtgg gtaacctgcc catcagaagg ggataacact 120 tggaaacagg tgctaatacc gtataacaat cgaaaccgca tggttttgat ttgaaaggcg 180 ctttcgggtg tcgctgatgg atggacccgc ggtgcattag ctagttggtg aggtaacggc 240 tcaccaaggc cacgatgcat agccgacctg agagggtgat cggccacatt gggactgaga 300 cacggcccaa actcctacgg gaggcagcag tagggaatct tcggcaatgg acgaaagtct 360 gaccgagcaa cgccgcgtga gtgaagaagg ttttcggatc gtaaaactct gttgttagag 420 aagaacaagg atgagagtaa ctgttcatcc cttgacggta tctaaccaga aagccacggc 480 taactacgtg ccagcagccg cggtaatacg taggtggcaa gcgttgtccg gatttattgg 540 gcgtaaagcg agcgcaggcg gtttcttaag tctgatgtga aagcccccgg ctcaaccggg 600 gagggtcatt ggaaactggg agacttgagt gcagaagagg agagtggaat tccatgtgta 660 gcggtgaaat gcgtagatat atggaggaac accagtggcg aaggcggctc tctggtctgt 720 aactgacgct gaggctcgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca 780 cgccgtaaac gatgagtgct aagtgttgga gggtttccgc ccttcagtgc tgcagctaac 840 gcattaagca ctccgcctgg ggagtacgac cgcaaggttg aaactcaaag gaattgacgg 900 gggcccgcac aagcggtgga gcatgtggtt taattcgaag caacgcgaag aaccttacca 960 ggtcttgaca tcctttgacc actctagaga tagagcttcc ccttcggggg caaagtgaca 1020 ggtggtgcat ggttgtcgtc agctcgtgtc gtgagatgtt gggttaattc ccgcaacgag 1080 cgcaacc 1087

<210> 28

<211> 1146

<212> DNA

<213> Enterococcus sp. isolate mc017cn_x_d2

<400> 28 ctatacatgc aagtcgtacg ctgtgctatt tgctcaccag gagcttgctc caccggaaaa 60 agaagagtgg cgaacgggtg agtaacacgt gggtaacctg cccatcagaa ggggataaca 120 cttggaaaca ggtgctaata ccgtataaca atcgaaaccg catggttttg atttgaaagg 180 cgctttcggg tgtcgctgat ggatggaccc gcggtgcatt agctagttgg tgaggtaacg 240 gctcaccaag gccacgatgc atagccgacc tgagagggtg atcggccaca ttgggactga 300 gacacggccc aaactcctac gggaggcagc agtagggaat cttcggcaat ggacgaaagt 360 ctgaccgagc aacgccgcgt gagtgaagaa ggttttcgga tcgtaaaact ctgttgttag 420 agaagaacaa ggatgagagt aactgttcat cccttgacgg tatctaacca gaaagccacg 480 gctaactacg tgccagcagc cgcggtaata cgtaggtggc aagcgttgtc cggatttatt 540 gggcgtaaag cgagcgcagg cggtttctta agtctgatgt gaaagccccc ggctcaaccg 600 gggagggtca ttggaaactg ggagacttga gtgcagaaga ggagagtgga attccatgtg 660 tagcggtgaa atgcgtagat atatggagga acaccagtgg cgaaggcggc tctctggtct 720 gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag gattagatac cctggtagtc 780 cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc gcccttcagt gctgcagcta 840 acgcattaag cactccgcct gggggagtac gaccgcaagg tttgaaactc aaaagaaatt 900 gacggggggc cccgcacaag ccggtggaag catgtgggtt taattcgaaa ccaacgcgaa 960 agaaccttta ccagggtctt ggacattcct ttggaccact tctagaagat agaagctttc 1020 cccctttcgg gggggcaaaa gtggacaagg tgggtggcat gggttggtcc atccaacttc 1080 ggtgttcgtg aagaatgttt ggggtttaaa gtccccgtca accaaggcgc caacccctta 1140 attggt 1146 <210> 29

<211> 1017

<212> DNA

<213> Enterococcus sp. isolate mc026cna2_e06

<220>

<221> misc_feature

<222> (136).. (136)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (772).. (772)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (877).. (877)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (905).. (905)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (921 ).. (921 )

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (924).. (924)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (926).. (926)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (931).. (931 )

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (944).. (944)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (954).. (954)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (973).. (974)

<223> n is a, c, g, or t

<220> <221> misc_feature

<222> (977).. (977)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (986).. (986)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (990).. (990)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (995).. (995)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (997).. (997)

<223> n is a, c, g, ort

<220>

<221> misc_feature

<222> (999).. (999)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (1001 ).. (1001 )

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (1003).. (1005)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (1008).. (1008)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (1011 ).. (1012)

<223> n is a, c, g, or t

<400> 29 tcgaacgctt ctttttccac cggagcttgc tccaccggaa aaagaggagt ggcgaacggg 60 tgagtaacac gtgggtaacc tgcccatcag aaggggataa cacttggaaa caggtgctaa 120 taccgtataa caatcnaaac cgcatggttt tgatttgaaa ggcgctttcg ggtgtcgctg 180 atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca aggccacgat 240 gcatagccga cctgagaggg tgatcggcca cattgggact gagacacggc ccaaactcct 300 acgggaggca gcagtaggga atcttcggca atggacgaaa gtctgaccga gcaacgccgc 360 gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac aaggatgaga 420 gtaactgttc atcccttgac ggtatctaac cagaaagcca cggctaacta cgtgccagca 480 gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca 540 ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac 600 tgggagactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg aaatgcgtag 660 atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggct 720 cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt anacgatgag 780 tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta agcactccgc 840 ctggggagta cgaccgcaag gttgaaactc aaagganttg acgggggccc gcacaagcgg 900 tggancatgt ggtttaattc naancnacgc naagaacctt accnggtctt gacntccttt 960 gaccactcta ganntanagc ttcccnttcn ggggncnang ngnnnggngg nngcatg 1017

<210> 30

<211> 987

<212> DNA

<213> Enterococcus sp. isolate mc026rna2_g06

<220>

<221> misc_feature

<222> (136).. (136)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (717).. (717)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (845).. (845)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (852).. (852)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (858).. (858)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (905).. (905)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (915).. (915)

<223> n is a, c, g, or t <220>

<221> misc_feature

<222> (918).. (919)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (921)..(921)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (926).. (926)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (934).. (934)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (937).. (937)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (944).. (944)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (954).. (954)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (971).. (971 )

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (973).. (973)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (977).. (978)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (982).. (982)

<223> n is a, c, g, or t

<400> 30 tcgaacgctt ctttttccac cggagcttgc tccaccggaa aaagaggagt ggcgaacggg 60 tgagtaacac gtgggtaacc tgcccatcag aaggggataa cacttggaaa caggtgctaa 120 taccgtataa caatcnaaac cgcatggttt tgatttgaaa ggcgctttcg ggtgtcgctg 180 atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca aggccacgat 240 gcatagccga cctgagaggg tgatcggcca cattgggact gagacacggc ccaaactcct 300 acgggaggca gcagtaggga atcttcggca atggacgaaa gtctgaccga gcaacgccgc 360 gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac aaggatgaga 420 gtaactgttc atcccttgac ggtatctaac cagaaagcca cggctaacta cgtgccagca 480 gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca 540 ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac 600 tgggagactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg aaatgcgtag 660 atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgangct 720 cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgag 780 tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta agcactccgc 840 ctggngagta cnaccgcnag gttgaaactc aaaggaattg acgggggccc gcacaagcgg 900 tggancatgt ggttnaannc naagcnacgc gaanaanctt accnggtctt gacntccttt 960 gaccactcta nanatanngc tncccct 987

<210> 31

<211> 1185

<212> DNA

<213> Enterococcus sp. isolate mc059rn_fx_g10

<400> 31 ctatacatgc agtcgtacgc ttctttttcc accggagctt gctccaccgg aaaaagagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggccacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 ataaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 900 ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960 ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa gtgacaggtg 1020 gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca 1080 acccttattg ttagttgcca tcattcagtt gggcactcta gcaagactgc cggtgacaaa 1140 ccggaggaag gtggggatga cgtcaaatca tcatgcccct tatga 1185

<210> 32

<211> 1075

<212> DNA

<213> Enterococcus sp. isolate mc017rn_x_f4

<400> 32 tgcagttcgt acgcttgcta ttttgctcca gcggagcttg ctccaccgga aaaagaagag 60 tggcgaacgg gtgagtaaca cgtgggtaac ctgcccatca gaaggggata acacttggaa 120 acaggtgcta ataccgtata acaatcgaaa ccgcatggtt ttgatttgaa aggcgctttc 180 gggtgtcgct gatggatgga cccgcggtgc attagctagt tggtgaggta acggctcacc 240 aaggccacga tgcatagccg acctgagagg gtgatcggcc acattgggac tgagacacgg 300 cccaaactcc tacgggaggc agcagtaggg aatcttcggc aatggacgaa agtctgaccg 360 agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt tagagaagaa 420 caaggatgag agtaactgtt catcccttga cggtatctaa ccagaaagcc acggctaact 480 acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggattt attgggcgta 540 aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg 600 tcattggaaa ctgggagact tgagtgcaga agaggagagt ggaattccat gtgtagcggt 660 gaaatgcgta gatatatgga ggaacaccag tggcgaaggc ggctctctgg tctgtaactg 720 acgctgaggc tcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccg 780 taaacgatga gtgctaagtg ttggagggtt tccgcccttc agtgctgcag ctaacgcatt 840 aagcactccg cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc 900 cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaggtct 960 tgacatcctt tgaccactct agagatagag cttccccttc gggggcaaag tgacaagtgg 1020 tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgag 1075

<210> 33

<211> 1000

<212> DNA

<213> Enterococcus sp. isolate mc017rn_12f_a

<220>

<221> misc_feature <222> (448).. (448)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (907).. (907)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (923).. (923)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (936).. (936)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (956).. (956)

<223> n is a, c, g, or t

<400> 33 agtcgtacgc ttctttttcc accggagctt gctccaccgg aaaaagaaga gtggcgaacg 60 ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga aacaggtgct 120 aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt cgggtgtcgc 180 tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac caaggccacg 240 atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg gcccaaactc 300 ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc gagcaacgcc 360 gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga acaaggatga 420 gagtaactgt tcatcccttg acggtatnta accagaaagc cacggctaac tacgtgccag 480 cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt aaagcgagcg 540 caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg gtcattggaa 600 actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg tgaaatgcgt 660 agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact gacgctgagg 720 ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc gtaaacgatg 780 agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat taagcactcc 840 gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc 900 ggtggancat gtggtttaat tcnaagcaac gcgaanaacc ttaccaggtc ttgacntcct 960 ttgaccactc tagagataga gcttcccctt cgggggcaaa 1000

<210> 34

<211> 998

<212> DNA

<213> Enterococcus sp. isolate mc017tn_12a_ma <220>

<221> misc_feature

<222> (446).. (446)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (871 ).. (873)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (905).. (905)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (945).. (945)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (990).. (990)

<223> n is a, c, g, or t

<400> 34 tcgtacgctt ctttttccac cggagcttgc tccaccggaa aaagaagagt ggcgaacggg 60 tgagtaacac gtgggtaacc tgcccatcag aaggggataa cacttggaaa caggtgctaa 120 taccgtataa caatcgaaac cgcatggttt tgatttgaaa ggcgctttcg ggtgtcgctg 180 atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca aggccacgat 240 gcatagccga cctgagaggg tgatcggcca cattgggact gagacacggc ccaaactcct 300 acgggaggca gcagtaggga atcttcggca atggacgaaa gtctgaccga gcaacgccgc 360 gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac aaggatgaga 420 gtaactgttc atcccttgac ggtatntaac cagaaagcca cggctaacta cgtgccagca 480 gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca 540 ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac 600 tgggagactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg aaatgcgtag 660 atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggct 720 cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgag 780 tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta agcactccgc 840 ctggggagta cgaccgcaag gttgaaactc nnngaattga cggggggccc gcacaagcgg 900 tggancatgt ggtttaattc gaagcaacgc gaagaacctt accangtctt gacatccttt 960 gaccactcta gagatagagc ttccccttcn ggggcaaa 998 <210> 35

<211> 1003

<212> DNA

<213> Enterococcus sp. isolate mc017tn_1 a_ma

<220>

<221> misc_feature

<222> (206).. (206)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (905).. (905)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (913).. (913)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (921).. (921 )

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (926).. (926)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (973)..(973)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (982).. (982)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (995).. (995)

<223> n is a, c, g, or t

<400> 35 tcgtacgctt ctttttccac cggagcttgc tccaccggaa aaagaagagt ggcgaacggg 60 tgagtaacac gtgggtaacc tgcccatcag aaggggataa cacttggaaa caggtgctaa 120 taccgtataa caatcgaaac cgcatggttt tgatttgaaa ggcgctttcg ggtgtcgctg 180 atggatggac ccgcggtgca ttagcnagtt ggtgaggtaa cggctcacca aggccacgat 240 gcatagccga cctgagaggg tgatcggcca cattgggact gagacacggc ccaaactcct 300 acgggaggca gcagtaggga atcttcggca atggacgaaa gtctgaccga gcaacgccgc 360 gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac aaggatgaga 420 gtaactgttc atcccttgac ggtatctaac cagaaagcca cggctaacta cgtgccagca 480 gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca 540 ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac 600 tgggagactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg aaatgcgtag 660 atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggct 720 cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgag 780 tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta agcactccgc 840 ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc gcacaagcgg 900 tggancatgt ggnttaattc naagcnacgc gaagaacctt accaggtctt gacatccttt 960 gaccactcta ganatagagc tnccccttcg ggggnaaagt gac 1003

<210> 36

<211> 999

<212> DNA

<213> Enterococcus sp. isolate mc017rn_12e_ma

<220>

<221> misc_feature

<222> (21 ).. (21 )

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (448).. (448)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (879).. (879)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (946).. (946)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (948).. (948)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (956).. (956)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (975).. (975)

<223> n is a, c, g, or t

<220>

<221> misc_feature

<222> (992).. (992) <223> n is a, c, g, or t

<400> 36 agtcgtacgc ttctttttcc nccggagctt gctccaccgg aaaaagaaga gtggcgaacg 60 ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga aacaggtgct 120 aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt cgggtgtcgc 180 tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac caaggccacg 240 atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg gcccaaactc 300 ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc gagcaacgcc 360 gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga acaaggatga 420 gagtaactgt tcatcccttg acggtatnta accagaaagc cacggctaac tacgtgccag 480 cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt aaagcgagcg 540 caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg gtcattggaa 600 actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg tgaaatgcgt 660 agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact gacgctgagg 720 ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc gtaaacgatg 780 agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat taagcactcc 840 gcctggggag tacgaccgca aggttgaaac tcaaaggant tgacgggggc ccgcacaagc 900 ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccngntc ttgacntcct 960 ttgaccactc taganataga gcttcccctt cnggggcaa 999

<210> 37

<211> 995

<212> DNA

<213> Enterococcus sp. isolate mc017cn_12c_ma

<220>

<221 > misc_feature

<222> (20).. (20)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (447).. (447)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (863).. (863)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (935).. (935)

<223> n is a, c, g, or t <220>

<221 > misc_feature

<222> (945).. (947)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (950).. (950)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (973).. (973)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (987).. (987)

<223> n is a, c, g, or t

<400> 37 gtcgtacgct tctttttccn ccggagcttg ctccaccgga aaaagaagag tggcgaacgg 60 gtgagtaaca cgtgggtaac ctgcccatca gaaggggata acacttggaa acaggtgcta 120 ataccgtata acaatcgaaa ccgcatggtt ttgatttgaa aggcgctttc gggtgtcgct 180 gatggatgga cccgcggtgc attagctagt tggtgaggta acggctcacc aaggccacga 240 tgcatagccg acctgagagg gtgatcggcc acattgggac tgagacacgg cccaaactcc 300 tacgggaggc agcagtaggg aatcttcggc aatggacgaa agtctgaccg agcaacgccg 360 cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt tagagaagaa caaggatgag 420 agtaactgtt catcccttga cggtatntaa ccagaaagcc acggctaact acgtgccagc 480 agccgcggta atacgtaggt ggcaagcgtt gtccggattt attgggcgta aagcgagcgc 540 aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg tcattggaaa 600 ctgggagact tgagtgcaga agaggagagt ggaattccat gtgtagcggt gaaatgcgta 660 gatatatgga ggaacaccag tggcgaaggc ggctctctgg tctgtaactg acgctgaggc 720 tcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccg taaacgatga 780 gtgctaagtg ttggagggtt tccgcccttc agtgctgcag ctaacgcatt aagcactccg 840 cctggggagt acgaccgcaa ggntgaaact caaaggaatt gacgggggcc cgcacaagcg 900 gtggagcatg tggtttaatt cgaagcaacg cgaanaacct taccnnnctn gacatccttt 960 gaccactcta ganatagagc ttccccntcg ggggc 995

<210> 38

<211> 993

<212> DNA

<213> Enterococcus sp. isolate mc026rnm2_a08 <220>

<221 > misc_feature

<222> (18).. (19)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (136).. (136)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (717).. (717)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (905).. (905)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (944).. (944)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (946).. (946)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (963).. (963)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (973).. (973)

<223> n is a, c, g, or t

<400> 38 tcgaacgctt ctttttcnnc cggagcttgc tccaccggaa aaagaggagt ggcgaacggg 60 tgagtaacac gtgggtaacc tgcccatcag aaggggataa cacttggaaa caggtgctaa 120 taccgtataa caatcnaaac cgcatggttt tgatttgaaa ggcgctttcg ggtgtcgctg 180 atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca aggccacgat 240 gcatagccga cctgagaggg tgatcggcca cattgggact gagacacggc ccaaactcct 300 acgggaggca gcagtaggga atcttcggca atggacgaaa gtctgaccga gcaacgccgc 360 gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac aaggatgaga 420 gtaactgttc atcccttgac ggtatctaac cagaaagcca cggctaacta cgtgccagca 480 gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca 540 ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac 600 tgggagactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg aaatgcgtag 660 atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgangct 720 cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgag 780 tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta agcactccgc 840 ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc gcacaagcgg 900 tggancatgt ggtttaattc gaagcaacgc gaagaacctt accngntctt gacatccttt 960 gancactcta ganatagagc ttccccttcg ggg 993

<210> 39

<211> 1067

<212> DNA

<213> Enterococcus sp. isolate mc017tn_x_a1

<400> 39 ctatacatgc agtcgtacgc tgtgcttttt ctcaccggag cttgctccac cggaaaaaga 60 agagtggcga acgggtgagt aacacgtggg taacctgccc atcagaaggg gataacactt 120 ggaaacaggt gctaataccg tataacaatc gaaaccgcat ggttttgatt tgaaaggcgc 180 tttcgggtgt cgctgatgga tggacccgcg gtgcattagc tagttggtga ggtaacggct 240 caccaaggcc acgatgcata gccgacctga gagggtgatc ggccacattg ggactgagac 300 acggcccaaa ctcctacggg aggcagcagt agggaatctt cggcaatgga cgaaagtctg 360 accgagcaac gccgcgtgag tgaagaaggt tttcggatcg taaaactctg ttgttagaga 420 agaacaagga tgagagtaac tgttcatccc ttgacggtat ctaaccagaa agccacggct 480 aactacgtgc cagcagccgc ggtaatacgt aggtggcaag cgttgtccgg atttattggg 540 cgtaaagcga gcgcaggcgg tttcttaagt ctgatgtgaa agcccccggc tcaaccgggg 600 agggtcattg gaaactggga gacttgagtg cagaagagga gagtggaatt ccatgtgtag 660 cggtgaaatg cgtagatata tggaggaaca ccagtggcga aggcggctct ctggtctgta 720 actgacgctg aggctcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac 780 gccgtaaacg atgagtgcta agtgttggag ggtttccgcc cttcagtgct gcagctaacg 840 cattaagcac tccgcctggg gagtacgacc gcaaggttga aactcaaagg aattgacggg 900 ggcccgcaca agcggtggag catgtggttt aattcgaagc aacgcgaaga accttaccag 960 gtcttgacat cctttgacca ctctagagat agagcttccc cttcgggggc aaagtgacag 1020 gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg ggttaag 1067

<210> 40

<211> 1210

<212> DNA

<213> Enterococcus sp. isolate mc127rifx_b11

<400> 40 ctatacgtgc agtcgtacgc ttcttttgcg caccggagct tgctccaccg gagaaagaag 60 agtggggaac gggtgagtag cacgtgggga acctgcccat cagaagggga taacacttgg 120 aaacaggtgc taataccgta taacaatcga aaccgcatgg ttttgatttg aaaggcgctt 180 tcgggtgtcg ctgatggatg gacccgcggt gcattagcta gttggtgagg taacggctca 240 ccaaggccac gatgcatagc cgacctgaga gggtgatcgg ccacattggg actgagacac 300 ggcccaaact cctacgggag gcagcagtag ggaatcttcg gcaatggacg aaagtctgac 360 cgagcaacgc cgcgtgagtg aagaaggttt tcggatcgta aaactctgtt gttagagaag 420 aacaaggatg agagtaactg ttcatccctt gacggtatct aaccagaaag ccacggctaa 480 ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg ttgtccggat ttattgggcg 540 taaagcgagc gcaggcggtt tcttaagtct gatgtgaaag cccccggctc aaccggggag 600 ggtcattgga aactgggaga cttgagtgca gaagaggaga gtggaattcc atgtgtagcg 660 gtgaaatgcg tagatatatg gaggaacacc agtggcgaag gcggctctct ggtctgtaac 720 tgacgctgag gctcgaaagc gtggggagca aacaggatta gataccctgg tagtccacgc 780 cgtaaacgat gagtgctaag tgttggaggg tttccgccct tcagtgctgc agctaacgca 840 ttaagcactc cgcctgggga gtacgaccgc aaggttgaaa ctcaaaggaa ttgacggggg 900 cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt 960 cttgacatcc tttgaccact ctagagatag agcttcccct tcgggggcaa agtgacaggt 1020 ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc 1080 aacccttatt gttagttgcc atcattcagt tgggcactct agcaagactg ccggtgacaa 1140 ccggaggaag gggggatgac gtcaaatcat catgcccctt atgacctggg ctaccacggg 1200 ctacatggga 1210

<210> 41

<211> 1001

<212> DNA

<213> Enterococcus sp. isolate mc026tnm2_c07

<220>

<221 > misc_feature

<222> (21 ).. (21 )

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (138).. (138)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (854).. (854)

<223> n is a, c, g, or t <220>

<221 > misc_feature

<222> (907).. (907)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (946).. (946)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (948).. (948)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (975).. (975)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (991).. (992)

<223> n is a, c, g, or t

<400> 41 agtcgaacgc ttctttttcc nccggagctt gctccaccgg aaaaagagga gtggcgaacg 60 ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga aacaggtgct 120 aataccgtat aacaatcnaa accgcatggt tttgatttga aaggcgcttt cgggtgtcgc 180 tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac caaggccacg 240 atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg gcccaaactc 300 ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc gagcaacgcc 360 gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga acaaggatga 420 gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac tacgtgccag 480 cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt aaagcgagcg 540 caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg gtcattggaa 600 actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg tgaaatgcgt 660 agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact gacgctgagg 720 ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc gtaaacgatg 780 agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat taagcactcc 840 gcctggggag tacnaccgca aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc 900 ggtggancat gtggtttaat tcgaagcaac gcgaagaacc ttaccngntc ttgacatcct 960 ttgaccactc taganataga gcttcccctt nngggggcaa a 1001

<210> 42

<211> 1151 <212> DNA

<213> Enterococcus sp. isolate mc097cnfx_h6

<400> 42 atacatgcag tcgaacgctt ctttttccac cggagcttgc tccaccggaa aagaggagtg 60 gcgaacgggt gagtaacacg tgggtaacct gcccatcaga aggggataac acttggaaac 120 aggtgctaat accgtataac aatcaaaacc gcatggtttt gatttgaaag gcgctttcgg 180 gtgtcgctga tggatggacc cgcggtgcat tagctagttg gtgaggtaac ggctcaccaa 240 ggccacgatg catagccgac ctgagagggt gatcggccac attgggactg agacacggcc 300 caaactccta cgggaggcag cagtagggaa tcttcggcaa tggacgaaag tctgaccgag 360 caacgccgcg tgagtgaaga aggttttcgg atcgtaaaac tctgttgtta gagaagaaca 420 aggatgagag taactgttca tcccttgacg gtatctaacc agaaagccac ggctaactac 480 gtgccagcag ccgcggtaat acgtaggtgg caagcgttgt ccggatttat tgggcgtaaa 540 gcgagcgcag gcggtttctt aagtctgatg tgaaagcccc cggctcaacc ggggagggtc 600 attggaaact gggagacttg agtgcagaag aggagagtgg aattccatgt gtagcggtga 660 aatgcgtaga tatatggagg aacaccagtg gcgaaggcgg ctctctggtc tgtaactgac 720 gctgaggctc gaaagcgtgg ggagcaaaca ggattagata ccctggtagt ccacgccgta 780 aacgatgagt gctaagtggt gggagggttt ccgcccttca gtgctgcagc taacgcatta 840 agcactccgc ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccg 900 cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg aagaacctta ccaggtcttg 960 acatcctttg accactctag agatagagct tccccttcgg gggcaagtga caggtggggg 1020 catggttgtc gtcagctcgt gtcgtgagat gtttgggtta agtcccgcaa cgagcgcaac 1080 ccttattggt agtgccatca ttcagttggg gactctacaa gaatgccgtg acaaccggag 1140 gaaggggggg g 1151

<210> 43

<211> 1149

<212> DNA

<213> Enterococcus sp. isolate mc127tnfx_c2

<400> 43 agtcgtacgc ttgctatttt ccaccggagc ttgcttccca ccggaaaaag aagagtggcg 60 aacgggtgag taacacgtgg gtaacctgcc catcagaagg ggataacact tggaaacagg 120 tgctaatacc gtataacaat cgaaaccgca tggttttgat ttgaaaggcg ctttcgggtg 180 tcgctgatgg atggacccgc ggtgcattag ctagttggtg aggtaacggc tcaccaaggc 240 cacgatgcat agccgacctg agagggtgat cggccacatt gggactgaga cacggcccaa 300 actcctacgg gaggcagcag tagggaatct tcggcaatgg acgaaagtct gaccgagcaa 360 cgccgcgtga gtgaagaagg ttttcggatc gtaaaactct gttgttagag aagaacaagg 420 atgagagtaa ctgttcatcc cttgacggta tctaaccaga aagccacggc taactacgtg 480 ccagcagccg cggtaatacg taggtggcaa gcgttgtccg gatttattgg gcgtaaagcg 540 agcgcaggcg gtttcttaag tctgatgtga aagcccccgg ctcaaccggg gagggtcatt 600 ggaaactggg agacttgagt gcagaagagg agagtggaat tccatgtgta gcggtgaaat 660 gcgtagatat atggaggaac accagtggcg aaggcggctc tctggtctgt aactgacgct 720 gaggctcgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccgtaaac 780 gatgagtgct aagtgttgga gggtttccgc ccttcagtgc tgcagctaac gcattaagca 840 ctccgcctgg ggagtacgac cgcaaggttg aaactcaaag gaattgacgg gggcccgcac 900 aagcggtgga gcatgtggtt taattcgaag caacgcgaag aaccttacca ggtcttgaca 960 tcctttgacc actctagaga tagagcttcc ccttcggggg caaagtgaca ggtggtgcat 1020 ggttgtcgtc acctcctgtc ctgaaaatgt tgggttaagt cccgcaacga acgcaaccct 1080 tattgttagt tgccatcatt cagttgggga ctctaccaag actgccggtg acaaaccgga 1140 ggaaggggg 1149

<210> 44

<211> 1114

<212> DNA

<213> Enterococcus sp. isolate mc127rifx_c10

<400> 44 ctatacgtgc agtcgtacgc ttctattttc caccggagct tgctccaccg gaaaaagaag 60 agtggcgaac gggtgagtaa cacgtgggta acctgcccat cagaagggga taacacttgg 120 aaacaggtgc taataccgta taacaatcga aaccgcatgg ttttgatttg aaaggcgctt 180 tcgggtgtcg ctgatggatg gacccgcggt gcattagcta gttggtgagg taacggctca 240 ccaaggccac gatgcatagc cgacctgaga gggtgatcgg ccacattggg actgagacac 300 ggcccaaact cctacgggag gcagcagtag ggaatcttcg gcaatggacg aaagtctgac 360 cgagcaacgc cgcgtgagtg aagaaggttt tcggatcgta aaactctgtt gttagagaag 420 aacaaggatg agagtaactg ttcatccctt gacggtatct aaccagaaag ccacggctaa 480 ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg ttgtccggat ttattgggcg 540 taaagcgagc gcaggcggtt tcttaagtct gatgtgaaag cccccggctc aaccggggag 600 ggtcattgga aactgggaga cttgagtgca gaagaggaga gtggaattcc atgtgtagcg 660 gtgaaatgcg tagatatatg gaggaacacc agtggcgaag gcggctctct ggtctgtaac 720 tgacgctgag gctcgaaagc gtggggagca aacaggatta gataccctgg tagtccacgc 780 cgtaaacgat gagtgctaag tgttggaggg tttccgccct tcagtgctgc agctaacgca 840 ttaagcactc cgcctgggga gtacgaccgc aaggttgaaa ctcaaaggaa ttgacggggg 900 cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt 960 cttgacatcc tttgaccact ctagagatag agcttcccct tcgggggcaa agtgacaggt 1020 ggtgcatggt tgtcgtcagc tcctgtcctg aaaatgttgg gttaagtccc gcaacgaccg 1080 caacccttat tgttagttgc catcattcag ttgg 1114

<210> 45

<211> 1211

<212> DNA

<213> Enterococcus sp. isolate CC00064

<400> 45 acatgcaagt cgaacgcttt ttctttcacc ggagcttgct ccaccgaaag aaaaggagtg 60 gcgaacgggt gagtaacacg tgggtaacct gcccatcaga aggggataac acttggaaac 120 aggtgctaat accgtataac aatcgaaacc gcatggtttc ggtttgaaag gcgcttttgc 180 gtcactgatg gatggacccg cggtgcatta gctagttggt gaggtaacgg ctcaccaagg 240 caacgatgca tagccgacct gagagggtga tcggccacat tgggactgag acacggccca 300 aactcctacg ggaggcagca gtagggaatc ttcggcaatg gacgcaagtc tgaccgagca 360 acgccgcgtg agtgaagaag gttttcggat cgtaaaactc tgttgttaga gaagaacaag 420 gatgagagta gaatgttcat cccttgacgg tatctaacca gaaagccacg gctaactacg 480 tgccagcagc cgcggtaata cgtaggtggc aagcgttgtc cggatttatt gggcgtaaag 540 cgagcgcagg cggtttctta agtctgatgt gaaagccccc ggctcaaccg gggagggtca 600 ttggaaactg ggaaacttga gtgcagaaga ggagagtgga attccatgtg tagcggtgaa 660 atgcgtagat atatggagga acaccagtgg cgaaggcggc tctctggtct gtaactgacg 720 ctgaggctcg aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa 780 acgatgagtg ctaagtgttg gagggtttcc gcccttcagt gctgcagcta acgcattaag 840 cactccgcct ggggagtacg accgcaaggt tgaaactcaa aggaattgac gggggcccgc 900 acaagcggtg gagcatgtgg tttaattcga agcaacgcga agaaccttac caggtcttga 960 catcctttga ccactctaga gatagagctt ccccttcggg ggcaaagtga caggtggtgc 1020 atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg agcgcaaccc 1080 ttattgttag ttgccatcat ttagttgggc actctagcga gactgccggt gacaaccgga 1140 ggaaggtggg gatgacgtca atcatcatgc cccttatgac cgggctacca cgtgctacat 1200 gggaagtaca c 1211

<210> 46

<211> 1176

<212> DNA <213> Enterococcus sp. isolate CC00262

<400> 46 ctatacatgc agtcgaacgc tttttctttc accggagctt gctccaccga agaaaaggag 60 tggcgaacgg gtgagtaaca cgtgggtaac ctgcccatca aaaggggata acacttggaa 120 acaggtgcta ataccgtata acaatagaaa ccgcagggtt tctatttgaa aggcgctttt 180 gcgtcactga tggatggacc cgcggtgcat tagctagttg gtgaggtaac ggctcaccaa 240 ggcaacgatg catagccaac ctgagagggt gatcggccac attgggactg agacacggcc 300 caaactccta cgggaggcag cagtagggaa tcttcggcaa tggacgaaag tctgaccgag 360 caacgccgcg tgagtgaaga aggttttcgg atcgtaaaac tctgttgtta gagaagaaca 420 aggatgagag tagaatgttc atcccttgac ggtatctaac cagaaagcca cggctaacta 480 cgtgccagca gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa 540 agcgagcgca ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt 600 cattggaaac tgggaaactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg 660 aaatgcgtag atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga 720 cgctgaggct cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt 780 aaacgatgag tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta 840 agcactccgc ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc 900 gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc gaagaacctt accaggtctt 960 gacatccttt gaccactcta gagatagagc ttccccttcg ggggcaaagt gacaggtggt 1020 gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac 1080 ccttattgtt agttgccatc atttagttgg gcactctagc gagactgccg gtgacaaccg 1140 gaggaaggtg gggatgacgt caaatcatca tgcccc 1176

<210> 47

<211> 1192

<212> DNA

<213> Enterococcus sp. isolate CC00264

<400> 47 ctatacatgc agtcgaacgc tttttctttc accggagctt gctccaccga agaaaaggag 60 tggcgaaggg gtgagtaaca cgtgggtaac ctgcccatca aaaggggata acacttggaa 120 acaggtgcta ataccgtata acaatagaaa ccgcagggtt tctatttgaa aggcgctttt 180 gcgtcactga tggatggacc cgcggtgcat tagctagttg gtgaggtaac ggctcaccaa 240 ggcaacgatg catagccaac ctgagagggt gatcggccac attgggactg agacacggcc 300 caaactccta cgggaggcag cagtagggaa tcttcggcaa tggacgaaag tctgaccgag 360 caacgccgcg tgagtgaaga aggttttcgg atcgtaaaac tctgttgtta gagaagaaca 420 aggatgagag tagaatgttc atcccttgac ggtatctaac cagaaagcca cggctaacta 480 cgtgccagca gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa 540 agcgagcgca ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt 600 cattggaaac tgggaaactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg 660 aaatgcgtag atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga 720 cgctaaggct cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt 780 aaacgatgag tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta 840 agcactccgc ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc 900 gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc gaagaacctt accaggtctt 960 gacatccttt gaccactcta gagatagagc ttccccttcg ggggcaaagt gacaggtggt 1020 gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac 1080 ccttattgtt agttgccatc atttagttgg gcactctagc gagactgccg gtgacaaacc 1140 ggaggaaggt ggggatgacg tcaaatcatc atgcccctta tgacctgggg ct 1192

<210> 48

<211> 1152

<212> DNA

<213> Enterococcus sp. isolate CC00263

<400> 48 ctatacatgc agtcgaacgc tttttctttc accggagctt gctccaccga agaaaaggag 60 tggcgaaggg gtgagtaaca cgtgggtaac ctgcccatca aaaggggata acacttggaa 120 acaggtgcta ataccgtata acaatagaaa ccgcagggtt tctatttgaa aggcgctttt 180 gcgtcactga tggatggacc cgcggtgcat tagctagttg gtgaggtaac ggctcaccaa 240 ggcaacgatg catagccaac ctgagagggt gatcggccac attgggactg agacacggcc 300 caaactccta cgggaggcag cagtagggaa tcttcggcaa tggacgaaag tctgaccgag 360 caacgccgcg tgagtgaaga aggttttcgg atcgtaaaac tctgttgtta gagaagaaca 420 aggatgagag tagaatgttc atcccttgac ggtatctaac cagaaagcca cggctaacta 480 cgtgccagca gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa 540 agcgagcgca ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt 600 cattggaaac tgggaaactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg 660 aaatgcgtag atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga 720 cgctaaggct cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt 780 aaacgatgag tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta 840 agcactccgc ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc 900 gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc gaagaacctt accaggtctt 960 gacatccttt gaccactcta gagatagagc ttccccttcg ggggcaaagt gacaggtggt 1020 gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac 1080 ccttattgtt agttgccatc atttatttgg gactctagcg agactgccgg tgacaaaccg 1140 aagaaaggtg gg 1152

<210> 49

<211> 1103

<212> DNA

<213> Enterococcus sp. isolate CC00619

<400> 49 ctatacatgc agtcgacgct ttttctttca ccggagcttg ctccaccgaa agaaaaggag 60 tggcgaacgg gtgagtaaca cgtgggtaac ctgcccatca gaaggggata acacttggaa 120 acaggtgcta ataccgtata acaatcgaaa ccgcatggtt tcggtttgaa aggcgctttt 180 gcgtcactga tggatggacc cgcggtgcat tagctagttg gtgaggtaac ggctcaccaa 240 ggcaacgatg catagccgac ctgagagggt gatcggccac attgggactg agacacggcc 300 caaactccta cgggaggcag cagtagggaa tcttcggcaa tggacgcaag tctgaccgag 360 caacgccgcg tgagtgaaga aggttttcgg atcgtaaaac tctgttgtta gagaagaaca 420 aggatgagag tagaatgttc atcccttgac ggtatctaac cagaaagcca cggctaacta 480 cgtgccagca gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa 540 agcgagcgca ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt 600 cattggaaac tgggaaactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg 660 aaatgcgtag atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga 720 cgctgaggct cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt 780 aaacgatgag tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta 840 agcactccgc ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc 900 gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc gaagaacctt accaggtctt 960 gacatccttt gaccactcta gagatagagc ttccccttcg ggggcaaagt gacaggtggt 1020 gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac 1080 ccttattgtt agttgccatc att 1103

<210> 50

<211> 1061

<212> DNA

<213> Enterococcus sp. isolate CC00002

<400> 50 ctatacatgc agtcgaacgc tttttctttc accggagctt gctccaccga aagaaaaaga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacactattt tccgcatgga agaaagttga aaggcgcttt 180 tgcgtcactg atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca 240 aggcaacgat gcatagccga cctgagaggg tgatcggcca cactgggact gagacacggc 300 ccagactcct acgggaggca gcagtaggga atcttcggca atggacgaaa gtctgaccga 360 gcaacgccgc gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac 420 aaggatgaga gtaaaatgtt catcccttga cggtatctaa ccagaaagcc acggctaact 480 acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggattt attgggcgta 540 aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg 600 tcattggaaa ctgggagact tgagtgcaga agaggagagt ggaattccat gtgtagcggt 660 gaaatgcgta gatatatgga ggaacaccag tggcgaaggc ggctctctgg tctgtaactg 720 acgctgaggc tcgaaagcgt ggggagcgaa caggattaga taccctggta gtccacgccg 780 tacacgatga gtgctaagtg ttggagggtt tccgcccttc agtgctgcag catacgcatt 840 aagcactccg cctggggagt acgaccgcaa ggttgagact caaaggaatt gacgggggcc 900 cgcacaagcg gtggagcatg tggtttatgt cgaagcaacg cgaagaacct taccaggtct 960 tgacatcctt tgaccactct acagatagag cttccccttc gggagcaaag tgacaggtgg 1020 tgcatgggtg tcttcgactc atgtcgtgaa aggtgggatt a 1061

<210> 51

<211> 1208

<212> DNA

<213> Enterococcus sp. isolate CC00066

<400> 51 ctatacatgc agtcgaacgc tttttctttc accggagctt gctccaccga aagaaaagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt ttcggtttga aaggcgcttt 180 tgcgtcactg atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca 240 aggcaacgat gcatagccga cctgagaggg tgatcggcca cattgggact gagacacggc 300 ccaaactcct acgggaggca gcagtaggga atcttcggca atggacgcaa gtctgaccga 360 gcaacgccgc gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac 420 aaggatgaga gtagaatgtt catcccttga cggtatctaa ccagaaagcc acggctaact 480 acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggattt attgggcgta 540 aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg 600 tcattggaaa ctgggaaact tgagtgcaga agaggagagt ggaattccat gtgtagcggt 660 gaaatgcgta gatatatgga ggaacaccag tggcgaaggc ggctctctgg tctgtaactg 720 acgctgaggc tcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccg 780 taaacgatga gtgctaagtg ttggagggtt tccgcccttc agtgctgcag ctaacgcatt 840 aagcactccg cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc 900 cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaggtct 960 tgacatcctt tgaccactct agagatagag cttccccttc gggggcaaag tgacaggtgg 1020 tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa 1080 cccttattgt tagttgccat catttagttg ggcactctag cgagactgcc ggtgacaacc 1140 ggaggaaggt ggggatgacg tcaatcatca tgccccttat gaccgggcta ccacgtgcta 1200 caatggga 1208

<210> 52

<211> 652

<212> DNA

<213> Enterococcus sp. isolate mc071tn_zx_b3

<400> 52 ctatacatgc agtcgaacgc tttttctttc accggagctt gctccaccga aagaaaaaga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacactattt tccgcatgga agaaagttga aaggcgcttt 180 tgcgtcactg atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca 240 aggcaacgat gcatagccga cctgagaggg tgatcggcca cactgggact gagacacggc 300 ccagactcct acgggaggca gcagtaggga atcttcggca atggacgaaa gtctgaccga 360 gcaacgccgc gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac 420 aaggatgaga gtaaaatgtt catcccttga cggtatctaa ccagaaagcc acggctaact 480 acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggattt attgggcgta 540 aagcgagcgc aggcggtttc ttaattctga tgtgaaagcc ccccggctca accggggagg 600 gtcattggaa actgggaaaa cttgagtggc agaagaggaa aaggggaatt tc 652

<210> 53

<211> 695

<212> DNA

<213> Enterococcus sp. isolate mc071cn_fx_e6

<400> 53 ctatacatgc agtcgaacgc tttttctttc accggagctt gctccaccga aagaaaaaga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacactattt tccgcatgga agaaagttga aaggcgcttt 180 tgcgtcactg atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca 240 aggcaacgat gcatagccga cctgagaggg tgatcggcca cactgggact gagacacggc 300 ccagactcct acgggaggca gcagtaggga atcttcggca atggacgaaa gtctgaccga 360 gcaacgccgc gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac 420 aaggatgaga gtaaaatgtt catcccttga cggtatctaa ccagaaagcc acggctaact 480 acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggattt attgggcgta 540 aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg 600 tcattggaaa ctgggagact tgagtgccga agaggatagt ggaattccat gtggatcgtt 660 gaaaggggta gatataggga ggaggactcc ctggt 695

<210> 54

<211> 1104

<212> DNA

<213> Enterococcus sp. isolate mc071cn_fx_f8

<400> 54 ctatacatgc agtcgaacgc tttttctttc accggagctt gctccaccga aagaaaaaga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacactattt tccgcatgga agaaagttga aaggcgcttt 180 tgcgtcactg atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca 240 aggcaacgat gcatagccga cctgagaggg tgatcggcca cactgggact gagacacggc 300 ccagactcct acgggaggca gcagtaggga atcttcggca atggacgaaa gtctgaccga 360 gcaacgccgc gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac 420 aaggatgaga gtaaaatgtt catcccttga cggtatctaa ccagaaagcc acggctaact 480 acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggattt attgggcgta 540 aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg 600 tcattggaaa ctgggagact tgagtgcaga agaggagagt ggaattccat gtgtagcggt 660 gaaatgcgta gatatatgga ggaacaccag tggcgaaggc ggctctctgg tctgtaactg 720 acgctgaggc tcgaaagcgt ggggagcgaa caggattaga taccctggta gtccacgccg 780 taaacgatga gtgctaagtg ttggagggtt tccgcccttc agtgctgcag caaacgcatt 840 aagcactccg cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc 900 cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaggtct 960 tgacatcctt tgaccactct agagatagag cttccccttc gggggcaaag tgacaggtgg 1020 tgcatggttg tcgtcacctc ctgtcctgaa atgttgggtt aagtcccgca acgagcgcac 1080 ccttattgtt agttgcctcc ttta 1104 no

<210> 55

<211> 975

<212> DNA

<213> Enterococcus sp. isolate mc001tny_c01

<220>

<221 > misc_feature

<222> (883).. (883)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (912).. (912)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (923).. (923)

<223> n is a, c, g, or t

<400> 55 ggagcttgct ccaccgaaag aaaaagagtg gcgaacgggt gagtaacacg tgggtaacct 60 gcccatcaga aggggataac acttggaaac aggtgctaat accgtataac actattttcc 120 gcatggaaga aagttgaaag gcgcttttgc gtcactgatg gatggacccg cggtgcatta 180 gctagttggt gaggtaacgg ctcaccaagg caacgatgca tagccgacct gagagggtga 240 tcggccacac tgggactgag acacggccca gactcctacg ggaggcagca gtagggaatc 300 ttcggcaatg gacgaaagtc tgaccgagca acgccgcgtg agtgaagaag gttttcggat 360 cgtaaaactc tgttgttaga gaagaacaag gatgagagta aaatgttcat cccttgacgg 420 tatctaacca gaaagccacg gctaactacg tgccagcagc cgcggtaata cgtaggtggc 480 aagcgttgtc cggatttatt gggcgtaaag cgagcgcagg cggtttctta agtctgatgt 540 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga 600 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg 660 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcgaacag 720 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc 780 gcccttcagt gctgcagcaa acgcattaag cactccgcct ggggagtacg accgcaaggt 840 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gancatgtgg tttaattcga 900 agcaacgcga anaaccttac cangtcttga catcctttga ccactctaga gatagagctt 960 ccccttcggg ggcaa 975

<210> 56

<211> 831

<212> DNA

<213> Enterococcus sp. isolate mc071tn_zx_d11 <400> 56 ctatacatgc agtcgaacgc tttttctttc accggagctt gctccaccga aagaaaaaga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacactattt tccgcatgga agaaagttga aaggcgcttt 180 tgcgtcactg atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca 240 aggcaacgat gcatagccga cctgagaggg tgatcggcca cactgggact gagacacggc 300 ccagactcct acgggaggca gcagtaggga atcttcggca atggacgaaa gtctgaccga 360 gcaacgccgc gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac 420 aaggatgaga gtaaaatgtt catcccttga cggtatctaa ccagaaagcc acggctaact 480 acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggattt attgggcgta 540 aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg 600 tcattggaaa ctgggagact tgagtgcaga agaggagagt ggaattccat gtgtagcggt 660 gaaatgcgta gatatatgga ggaacaccag tggcgaaggc ggctctctgg tctgtaactg 720 acgctgaggc tcgaaagcgt ggggagcgaa cagggattag ataccatggt agtccactcc 780 cgtaaaccga tgagtgctaa ttgttggagg gtttacgtcc ctccagggct g 831

<210> 57

<211> 898

<212> DNA

<213> Enterococcus sp. isolate mc071tn_zx_d8

<400> 57 gcgggctata catgcagtcg aacgcttttt ctttcaccgg agcttgctcc accgaaagaa 60 aaagagtggc gaacgggtga gtaacacgtg ggtaacctgc ccatcagaag gggataacac 120 ttggaaacag gtgctaatac cgtataacac tattttccgc atggaagaaa gttgaaaggc 180 gcttttgcgt cactgatgga tggacccgcg gtgcattagc tagttggtga ggtaacggct 240 caccaaggca acgatgcata gccgacctga gagggtgatc ggccacactg ggactgagac 300 acggcccaga ctcctacggg aggcagcagt agggaatctt cggcaatgga cgaaagtctg 360 accgagcaac gccgcgtgag tgaagaaggt tttcggatcg taaaactctg ttgttagaga 420 agaacaagga tgagagtaaa atgttcatcc cttgacggta tctaaccaga aagccacggc 480 taactacgtg ccagcagccg cggtaatacg taggtggcaa gcgttgtccg gatttattgg 540 gcgtaaagcg agcgcaggcg gtttcttaag tctgatgtga aagcccccgg ctcaaccggg 600 gagggtcatt ggaaactggg agacttgagt gcagaagagg agagtggaat tccatgtgta 660 gcggtgaaat gcgtagatat atggaggaac accagtggcg aaggcggctc tctggtctgt 720 aactgacgct gaggctcgaa agcgtgcgga gcgaacagga ttagatactc tggtagtcca 780 cgccgtacac gatgagtgct atgtgttgga gggtttccgc tcttcagtgc tgcagcacac 840 acattaagca ctccgcctgg ggagtacgac cgccggttga gactcaaagg aattgaca 898

<210> 58

<211> 996

<212> DNA

<213> Enterococcus sp. isolate mc026tna2_b06

<220>

<221 > misc_feature

<222> (19).. (19)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (422).. (422)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (425).. (425)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (904).. (904)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (920).. (920)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (933).. (933)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (972).. (972)

<223> n is a, c, g, or t

<400> 58 tcgaacgctt tttctttcnc cggagcttgc tccaccgaaa gaaaaggagt ggcgaacggg 60 tgagtaacac gtgggtaacc tgcccatcag aaggggataa cacttggaaa caggtgctaa 120 taccgtataa caatcgaaac cgcatggttt cggtttgaaa ggcgcttttg cgtcactgat 180 ggatggaccc gcggtgcatt agctagttgg tgaggtaacg gctcaccaag gcaacgatgc 240 atagccgacc tgagagggtg atcggccaca ttgggactga gacacggccc aaactcctac 300 gggaggcagc agtagggaat cttcggcaat ggacgcaagt ctgaccgagc aacgccgcgt 360 gagtgaagaa ggttttcgga tcgtaaaact ctgttgttag agaagaacaa ggatgagagt 420 anaangttca tcccttgacg gtatctaacc agaaagccac ggctaactac gtgccagcag 480 ccgcggtaat acgtaggtgg caagcgttgt ccggatttat tgggcgtaaa gcgagcgcag 540 gcggtttctt aagtctgatg tgaaagcccc cggctcaacc ggggagggtc attggaaact 600 gggaaacttg agtgcagaag aggagagtgg aattccatgt gtagcggtga aatgcgtaga 660 tatatggagg aacaccagtg gcgaaggcgg ctctctggtc tgtaactgac gctgaggctc 720 gaaagcgtgg ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgagt 780 gctaagtgtt ggagggtttc cgcccttcag tgctgcagct aacgcattaa gcactccgcc 840 tggggagtac gaccgcaagg ttgaaactca aaggaattga cgggggcccg cacaagcggt 900 ggancatgtg gtttaattcn aagcaacgcg aanaacctta ccaggtcttg acatcctttg 960 accactctag anatagagct tccccttcgg gggcaa 996

<210> 59

<211> 808

<212> DNA

<213> Enterococcus sp. isolate mc071tn_zx_c7

<400> 59 ctatacatgc agtcgaacgc tttttctttc accggagctt gctccaccga aagaaaagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt ttcggtttga aaggcgcttt 180 tgcgtcactg atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca 240 aggcaacgat gcatagccga cctgagaggg tgatcggcca cattgggact gagacacggc 300 ccaaactcct acgggaggca gcagtaggga atcttcggca atggacgcaa gtctgaccga 360 gcaacgccgc gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac 420 aaggatgaga gtagaatgtt catcccttga cggtatctaa ccagaaagcc acggctaact 480 acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggattt attgggcgta 540 aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg 600 tcattggaaa ctgggaaact tgagtgcaga agaggagagt ggaattccat gtgtagcggt 660 gaaatgcgta gatatatgga ggaacaccag tggcgaaagg cggctctctg ggtctgtaac 720 tgaacgctga aggctcgaaa agcgtggggg agcaaacagg aattagaata ccctgggtag 780 tccacgcccg taaaccaatg aagggcta 808

<210> 60

<211> 831

<212> DNA

<213> Enterococcus sp. isolate mc071cn_fx_h7

<400> 60 ctatacatgc aagtcgaacg ctttttcttt caccggagct tgctccaccg aaagaaaagg 60 agtggcgaac gggtgagtaa cacgtgggta acctgcccat cagaagggga taacacttgg 120 aaacaggtgc taataccgta taacaatcga aaccgcatgg tttcggtttg aaaggcgctt 180 ttgcgtcact gatggatgga cccgcggtgc attagctagt tggtgaggta acggctcacc 240 aaggcaacga tgcatagccg acctgagagg gtgatcggcc acattgggac tgagacacgg 300 cccaaactcc tacgggaggc agcagtaggg aatcttcggc aatggacgca agtctgaccg 360 agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt tagagaagaa 420 caaggatgag agtagaatgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actggggaaa cttgagtgca gaagaggaga gtggaattcc atgtgtagcg 660 gtgaaatgcg tagatatatg gaggaacacc agtggcgaag gcggctctct ggtctgtaac 720 tgacgctaag gctcgaaagc gtgggaagca aaccggaatt agataccctg ggtagtccac 780 gcccgtaaaa cgaatgagtt gctaaagggt tggaagggtt tccgcccttt c 831

<210> 61

<211> 793

<212> DNA

<213> Enterococcus sp. isolate mc071tn_fx_c5

<400> 61 ctatacatgc aagtcgacgc tttttctttc accggagctt gctccaccga aagaaaagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt ttcggtttga aaggcgcttt 180 tgcgtcactg atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca 240 aggcaacgat gcatagccga cctgagaggg tgatcggcca cattgggact gagacacggc 300 ccaaactcct acgggaggca gcagtaggga atcttcggca atggacgcaa gtctgaccga 360 gcaacgccgc gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac 420 aaggatgaga gtagaatgtt catcccttga cggtatctaa ccagaaagcc acggctaact 480 acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggattt attgggcgta 540 aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg 600 tcattggaaa ctgggaaact tgagtgcaga aaaaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaaag gcggctctct ggtctgtaac 720 tgacgctgaa gctccaaaag cgtgggggag ccaacgggat tagatacccc ggggagtcca 780 cgcccgtaaa aca 793

<210> 62

<211> 804 <212> DNA

<213> Enterococcus sp. isolate mc071tn_zx_b4

<400> 62 ctatacatgc agtcgacgct ttttctttca ccggagcttg ctccaccgaa agaaaaggag 60 tggcgaacgg gtgagtaaca cgtgggtaac ctgcccatca gaaggggata acacttggaa 120 acaggtgcta ataccgtata acaatcgaaa ccgcatggtt tcggtttgaa aggcgctttt 180 gcgtcactga tggatggacc cgcggtgcat tagctagttg gtgaggtaac ggctcaccaa 240 ggcaacgatg catagccgac ctgagagggt gatcggccac attgggactg agacacggcc 300 caaactccta cgggaggcag cagtagggaa tcttcggcaa tggacgcaag tctgaccgag 360 caacgccgcg tgagtgaaga aggttttcgg atcgtaaaac tctgttgtta gagaagaaca 420 aggatgagag tagaatgttc atcccttgac ggtatctaac cagaaagcca cggctaacta 480 cgtgccagca gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa 540 agcgagcgca ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt 600 cattggaaac tggggaaact tgagtgcaga aaaggagagt ggaaattcca tgtgtagcgg 660 tgaaaatgcg tagatatatg gaggaacacc agtggcgaaa ggcggctctt tggtttgtaa 720 actgaacgct taaggctcca aaagcgtggg ggagccaaac cgggattaga atacccctgg 780 gtagtccccg cccgtaaaac caat 804

<210> 63

<211> 1091

<212> DNA

<213> Enterococcus sp. isolate mc071tn_fx_d4

<400> 63 ctaatacatg cagtcgaacg ctttttcttt caccggagct tgctccaccg aaagaaaagg 60 agtggcgaac gggtgagtaa cacgtgggta acctgcccat cagaagggga taacacttgg 120 aaacaggtgc taataccgta taacaatcga aaccgcatgg tttcggtttg aaaggcgctt 180 ttgcgtcact gatggatgga cccgcggtgc attagctagt tggtgaggta acggctcacc 240 aaggcaacga tgcatagccg acctgagagg gtgatcggcc acattgggac tgagacacgg 300 cccaaactcc tacgggaggc agcagtaggg aatcttcggc aatggacgca agtctgaccg 360 agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt tagagaagaa 420 caaggatgag agtaaaatgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggaaac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 900 ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960 ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa gtgacaggtg 1020 gtgcatggtt gtcgtcagct cgtgtcggga gatgttgggt taagtcccgc aacgagggca 1080 acccttatgg t 1091

<210> 64

<211> 1149

<212> DNA

<213> Enterococcus sp. isolate mc071tn_fx_d5

<400> 64 catgcagtcg aacgcttttt ctttcaccgg agcttgctcc accgaaagaa aaggagtggc 60 gaacgggtga gtaacacgtg ggtaacctgc ccatcagaag gggataacac ttggaaacag 120 gtgctaatac cgtataacaa tcgaaaccgc atggtttcgg tttgaaaggc gcttttgcgt 180 cactgatgga tggacccgcg gtgcattagc tagttggtga ggtaacggct caccaaggca 240 acgatgcata gccgacctga gagggtgatc ggccacattg ggactgagac acggcccaaa 300 ctcctacggg aggcagcagt agggaatctt cggcaatgga cgcaagtctg accgagcaac 360 gccgcgtgag tgaagaaggt tttcggatcg taaaactctg ttgttagaga agaacaagga 420 tgagagtaaa atgttcatcc cttgacggta tctaaccaga aagccacggc taactacgtg 480 ccagcagccg cggtaatacg taggtggcaa gcgttgtccg gatttattgg gcgtaaagcg 540 agcgcaggcg gtttcttaag tctgatgtga aagcccccgg ctcaaccggg gagggtcatt 600 ggaaactggg aaacttgagt gcagaagagg agagtggaat tccatgtgta gcggtgaaat 660 gcgtagatat atggaggaac accagtggcg aaggcggctc tctggtctgt aactgacgct 720 gaggctcgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccgtaaac 780 gatgagtgct aagtgttgga gggtttccgc ccttcagtgc tgcagctaac gcattaagca 840 ctccgcctgg ggagtacgac cgcaaggttg aaactcaaag gaattgacgg gggcccgcac 900 aagcggtgga gcatgtggtt taattcgaag caacgcgaag aaccttacca ggtcttgaca 960 tcctttgacc actctagaga tagagcttcc ccttcggggg caaagtgaca ggtggtgcat 1020 ggttgtcgtc agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag cgcaaccctt 1080 attgttagtt gccatcattt aattgggcac tctagcgaga ctgccggtga caaaccggag 1140 gaaaggggg 1149

<210> 65 <211> 987

<212> DNA

<213> Enterococcus sp. isolate mc026cnm1_e07

<220>

<221 > misc_feature

<222> (423).. (423)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (426).. (426)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (832).. (832)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (860).. (861 )

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (916).. (916)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (922).. (922)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (930).. (930)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (932).. (932)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (935).. (935)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (937).. (937)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (939).. (939)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (945).. (947) <223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (954).. (954)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (971).. (971 )

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (973).. (973)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (975).. (975)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (977).. (979)

<223> n is a, c, g, or t

<400> 65 gtcgaacgct ttttctttca ccggagcttg ctccaccgaa agaaaaggag tggcgaacgg 60 gtgagtaaca cgtgggtaac ctgcccatca gaaggggata acacttggaa acaggtgcta 120 ataccgtata acaatcgaaa ccgcatggtt tcggtttgaa aggcgctttt gcgtcactga 180 tggatggacc cgcggtgcat tagctagttg gtgaggtaac ggctcaccaa ggcaacgatg 240 catagccgac ctgagagggt gatcggccac attgggactg agacacggcc caaactccta 300 cgggaggcag cagtagggaa tcttcggcaa tggacgcaag tctgaccgag caacgccgcg 360 tgagtgaaga aggttttcgg atcgtaaaac tctgttgtta gagaagaaca aggatgagag 420 tanaangttc atcccttgac ggtatctaac cagaaagcca cggctaacta cgtgccagca 480 gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca 540 ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac 600 tgggaaactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg aaatgcgtag 660 atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggct 720 cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgag 780 tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta ancactccgc 840 ctgggggagt acgaccgcan ngttgaaact caaaggaatt gacgggggcc cgcacaagcg 900 gtggagcatg tggttnaatt cnaagcaacn cnaanancnt taccnnnctt gacntccttt 960 gaccactcta nananannnc ttcccct 987 <210> 66

<211> 1081

<212> DNA

<213> Enterococcus sp. isolate mc071tn_fx_c10

<400> 66 ctaataatgc aagtcgaacg ctttttcttt caccggagct tgctccaccg aaagaaaagg 60 agtggcgaac gggtgagtaa cacgtgggta acctgcccat cagaagggga taacacttgg 120 aaacaggtgc taataccgta taacaatcga aaccgcatgg tttcggtttg aaaggcgctt 180 ttgcgtcact gatggatgga cccgcggtgc attagctagt tggtgaggta acggctcacc 240 aaggcaacga tgcatagccg acctgagagg gtgatcggcc acattgggac tgagacacgg 300 cccaaactcc tacgggaggc agcagtaggg aatcttcggc aatggacgca agtctgaccg 360 agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt tagagaagaa 420 caaggatgag agtaaaatgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggaaac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 900 ccgcacaagc ggtggagcat gtgggtttaa ttcgaaagca acgcgaaaga accttaccag 960 ggtcttgaca tccttttgac cactctagaa gatagaagct tcccccttcg gggggcaaaa 1020 gtgaccaggt gggtgcatgg gttgtccgtc cacctccggg tccgggaaaa tgttggggtt 1080 a 1081

<210> 67

<211> 1028

<212> DNA

<213> Enterococcus sp. isolate mc071tn_fx_a9

<400> 67 gcgggcctat acatgcagtc gaacgctttt tctttcaccg gagcttgctc caccgaaaga 60 aaaggagtgg cgaacgggtg agtaacacgt gggtaacctg cccatcagaa ggggataaca 120 cttggaaaca ggtgctaata ccgtataaca atcgaaaccg catggtttcg gtttgaaagg 180 cgcttttgcg tcactgatgg atggacccgc ggtgcattag ctagttggtg aggtaacggc 240 tcaccaaggc aacgatgcat agccgacctg agagggtgat cggccacatt gggactgaga 300 cacggcccaa actcctacgg gaggcagcag tagggaatct tcggcaatgg acgcaagtct 360 gaccgagcaa cgccgcgtga gtgaagaagg ttttcggatc gtaaaactct gttgttagag 420 aagaacaagg atgagagtaa aatgttcatc ccttgacggt atctaaccag aaagccacgg 480 ctaactacgt gccagcagcc gcggtaatac gtaggtggca agcgttgtcc ggatttattg 540 ggcgtaaagc gagcgcaggc ggtttcttaa gtctgatgtg aaagcccccg gctcaaccgg 600 ggagggtcat tggaaactgg gaaacttgag tgcagaagag gagagtggaa ttccatgtgt 660 agcggtgaaa tgcgtagata tatggaggaa caccagtggc gaaggcggct ctctggtctg 720 taactgacgc tgaggctcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc 780 acgccgtaaa cgatgagtgc taagtgttgg agggtttccg cccttcagtg ctgcagctaa 840 cgcattaagc actccgcctg gggagtacga ccgcaaggtt gaaactcaaa ggaattgacg 900 ggggcccgca caagcgggtg gaacatgtgg gtttaattcg aaagcaacgc gaagaaacct 960 taccagggtc ttgacatcct ttgaccactc tagaaaataa aagcttcccc cttcgggggg 1020 caaaagtg 1028

<210> 68

<211> 802

<212> DNA

<213> Enterococcus sp. isolate mc071tn_fx_a4

<400> 68 ctaatacatg cagtcgaacg ctttttcttt caccggagct tgctccaccg aaagaaaagg 60 agtggcgaac gggtgagtaa cacgtgggta acctgcccat cagaagggga taacacttgg 120 aaacaggtgc taataccgta taacaatcga aaccgcatgg tttcggtttg aaaggcgctt 180 ttgcgtcact gatggatgga cccgcggtgc attagctagt tggtgaggta acggctcacc 240 aaggcaacga tgcatagccg acctgagagg gtgatcggcc acattgggac tgagacacgg 300 cccaaactcc tacgggaggc agcagtaggg aatcttcggc aatggacgca agtctgaccg 360 agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt tagagaagaa 420 caaggatgag agtaaaatgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggaaac ttgagtgcaa aagaggagag tggaattcca tgtgtagcgg 660 tggaaatgcg tagatatatg gaggaacacc agtgggcgaa aggcggctct ctgggtctgt 720 aaactgaacg ctgaaggctc gaaaagcgtg ggggaagaaa aacagggatt tagatacccc 780 tgggtaactc ccccgccgta aa 802

<210> 69

<211> 919

<212> DNA <213> Enterococcus sp. isolate mc071tn_zx_c2

<400> 69 ctatacatgc aagtcgaacg ctttttcttt caccggagct tgctccaccg aaagaaaagg 60 agtggcgaac gggtgagtaa cacgtgggta acctgcccat cagaagggga taacacttgg 120 aaacaggtgc taataccgta taacaatcga aaccgcatgg tttcggtttg aaaggcgctt 180 ttgcgtcact gatggatgga cccgcggtgc attagctagt tggtgaggta acggctcacc 240 aaggcaacga tgcatagccg acctgagagg gtgatcggcc acattgggac tgagacacgg 300 cccaaactcc tacgggaggc agcagtaggg aatcttcggc aatggacgca agtctgaccg 360 agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt tagagaagaa 420 caaggatgag agtaaaatgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggaaac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggaa taccaaccgc aagggttgaa actcaaggga attgaagggg 900 ggccccccca aaccggtgg 919

<210> 70

<211> 975

<212> DNA

<213> Enterococcus sp. isolate mc071cn_zx_g1

<400> 70 ctaatacatg cagtcgaacg ctttttcttt caccggagct tgctccaccg aaagaaaagg 60 agtggcgaac gggtgagtaa cacgtgggta acctgcccat cagaagggga taacacttgg 120 aaacaggtgc taataccgta taacaatcga aaccgcatgg tttcggtttg aaaggcgctt 180 ttgcgtcact gatggatgga cccgcggtgc attagctagt tggtgaggta acggctcacc 240 aaggcaacga tgcatagccg acctgagagg gtgatcggcc acattgggac tgagacacgg 300 cccaaactcc tacgggaggc agcagtaggg aatcttcggc aatggacgca agtctgaccg 360 agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt tagagaagaa 420 caaggatgag agtaaaatgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggaaac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggga gtaccaaccg caaggttgaa actcaaagga attgacgggg 900 ggcccgccac aagcggtgga agcagggtgg tttaaattcg aaagcaaccc cgaagaaacc 960 tttaccaggg tcttg 975

<210> 71

<211> 1074

<212> DNA

<213> Enterococcus sp. isolate mc071tn_zx_b7

<400> 71 ctatacatgc aagtcgaacg ctttttcttt caccggagct tgctccaccg aaagaaaagg 60 agtggcgaac gggtgagtaa cacgtgggta acctgcccat cagaagggga taacacttgg 120 aaacaggtgc taataccgta taacaatcga aaccgcatgg tttcggtttg aaaggcgctt 180 ttgcgtcact gatggatgga cccgcggtgc attagctagt tggtgaggta acggctcacc 240 aaggcaacga tgcatagccg acctgagagg gtgatcggcc acattgggac tgagacacgg 300 cccaaactcc tacgggaggc agcagtaggg aatcttcggc aatggacgca agtctgaccg 360 agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt tagagaagaa 420 caaggatgag agtaaaatgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggaaac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 900 ccgcacaagc cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt 960 cttgacatcc tttgaccact ctagagatag agcttcccct tcgggggcaa agtgacaggt 1020 ggtgcatggt tgtcgtcacc tccggtcctg gaaatgttgg gttaagtccc gcaa 1074

<210> 72

<211> 1064

<212> DNA

<213> Enterococcus sp. isolate mc071tn_fx_c1

<400> 72 gcaagtcgaa cgctttttct ttcaccggag cttgctccac cgaaagaaaa ggagtggcga 60 acgggtgagt aacacgtggg taacctgccc atcagaaggg gataacactt ggaaacaggt 120 gctaataccg tataacaatc gaaaccgcat ggtttcggtt tgaaaggcgc ttttgcgtca 180 ctgatggatg gacccgcggt gcattagcta gttggtgagg taacggctca ccaaggcaac 240 gatgcatagc cgacctgaga gggtgatcgg ccacattggg actgagacac ggcccaaact 300 cctacgggag gcagcagtag ggaatcttcg gcaatggacg caagtctgac cgagcaacgc 360 cgcgtgagtg aagaaggttt tcggatcgta aaactctgtt gttagagaag aacaaggatg 420 agagtagaat gttcatccct tgacggtatc taaccagaaa gccacggcta actacgtgcc 480 agcagccgcg gtaatacgta ggtggcaagc gttgtccgga tttattgggc gtaaagcgag 540 cgcaggcggt ttcttaagtc tgatgtgaaa gcccccggct caaccgggga gggtcattgg 600 aaactgggaa acttgagtgc agaagaggag agtggaattc catgtgtagc ggtgaaatgc 660 gtagatatat ggaggaacac cagtggcgaa ggcggctctc tggtctgtaa ctgacgctga 720 ggctcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg ccgtaaacga 780 tgagtgctaa gtgttggagg gtttccgccc ttcagtgctg cagctaacgc attaagcact 840 ccgcctgggg gagtacgacc gcaaggttga aactcagagg aattgacggg ggcccgcaca 900 agcggtggag catgtggttt aattcgaacc aacgcgaaga accttaccag gtcttgacat 960 cctttgacca ctccagaaaa tagagcttcc ccttcggggg cacagtgaaa gtggtgcatg 1020 gttgtcgtca cctcgtgtcg ggaaaagttg ggttaagtcc ccca 1064

<210> 73

<211> 992

<212> DNA

<213> Enterococcus sp. isolate mc026cna1_d06

<220>

<221 > misc_feature

<222> (19).. (19)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (422).. (422)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (425).. (425)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (943).. (945)

<223> n is a, c, g, or t <220>

<221 > misc_feature

<222> (971).. (971 )

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (987).. (987)

<223> n is a, c, g, or t

<400> 73 tcgaacgctt tttctttcnc cggagcttgc tccaccgaaa gaaaaggagt ggcgaacggg 60 tgagtaacac gtgggtaacc tgcccatcag aaggggataa cacttggaaa caggtgctaa 120 taccgtataa caatcgaaac cgcatggttt cggtttgaaa ggcgcttttg cgtcactgat 180 ggatggaccc gcggtgcatt agctagttgg tgaggtaacg gctcaccaag gcaacgatgc 240 atagccgacc tgagagggtg atcggccaca ttgggactga gacacggccc aaactcctac 300 gggaggcagc agtagggaat cttcggcaat ggacgcaagt ctgaccgagc aacgccgcgt 360 gagtgaagaa ggttttcgga tcgtaaaact ctgttgttag agaagaacaa ggatgagagt 420 anaangttca tcccttgacg gtatctaacc agaaagccac ggctaactac gtgccagcag 480 ccgcggtaat acgtaggtgg caagcgttgt ccggatttat tgggcgtaaa gcgagcgcag 540 gcggtttctt aagtctgatg tgaaagcccc cggctcaacc ggggagggtc attggaaact 600 gggaaacttg agtgcagaag aggagagtgg aattccatgt gtagcggtga aatgcgtaga 660 tatatggagg aacaccagtg gcgaaggcgg ctctctggtc tgtaactgac gctgaggctc 720 gaaagcgtgg ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgagt 780 gctaagtgtt ggagggtttc cgcccttcag tgctgcagct aacgcattaa gcactccgcc 840 tggggagtac gaccgcaagg ttgaaactca aaggaattga cgggggcccg cacaagcggt 900 ggagcatgtg gtttaattcg aagcaacgcg aagaacctta ccnnncttga catcctttga 960 ccactctaga natagagctt ccccttnggg gg 992

<210> 74

<211> 1075

<212> DNA

<213> Enterococcus sp. isolate mc071tn_fx_b1

<400> 74 ctatacatgc agtcgaacgc tttttctttc accggagctt gctccaccga aagaaaagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt ttcggtttga aaggcgcttt 180 tgcgtcactg atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca 240 aggcaacgat gcatagccga cctgagaggg tgatcggcca cattgggact gagacacggc 300 ccaaactcct acgggaggca gcagtaggga atcttcggca atggacgcaa gtctgaccga 360 gcaacgccgc gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac 420 aaggatgaga gtaaaatgtt catcccttga cggtatctaa ccagaaagcc acggctaact 480 acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggattt attgggcgta 540 aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg 600 tcattggaaa ctgggaaact tgagtgcaga agaggagagt ggaattccat gtgtagcggt 660 gaaatgcgta gatatatgga ggaacaccag tggcgaaggc ggctctctgg tctgtaactg 720 acgctgaggc tcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccg 780 taaacgatga gtgctaagtg ttggagggtt tccgcccttc agtgctgcag ctaacgcatt 840 aagcactccg cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc 900 cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaggtct 960 tgacatcctt tgaccactct agagatagag cttccccttc gggggcaaag tgacaggtgg 1020 tgcatggttg tcgtcacctc gtgtcctgag atgttgggtt aagtcccgca acaac 1075

<210> 75

<21 1 > 24

<212> DNA

<213> Artificial sequence 1

<220>

<223> Artifical sequence

<400> 75 cgaggtacac cgtaagttgt tgct 24

<210> 76

<21 1 > 24

<212> DNA

<213> Artificial sequence2

<220>

<223> Artifical sequence

<400> 76 accaggacac gtagacttca cagt 24

<210> 77

<21 1 > 23

<212> DNA

<213> Artificial sequences

<220>

<223> Artifical sequence

<400> 77 tggcccaaag atacgtgttc cac 23

<210> 78 <211> 24

<212> DNA

<213> Artificial sequence4

<220>

<223> Artifical sequence

<400> 78 cgaacaaaat caggagctcg tcgt 24

<210> 79

<211> 20

<212> DNA

<213> Artificial sequence5

<220>

<223> Artifical sequence

<400> 79 actgctgagt gcccacgctt 20

<210> 80

<211> 22

<212> DNA

<213> Artificial sequence6

<220>

<223> Artifical sequence

<400> 80 gaggcacttg ctagcgtcac ac 22

<210> 81

<211> 22

<212> DNA

<213> Artificial sequence7

<220>

<223> Artifical sequence

<400> 81 tgcgtgtagg catcatccgt ga 22

<210> 82

<211> 21

<212> DNA

<213> Artificial sequence8

<220>

<223> Artifical sequence

<400> 82 gacacagcgg catcagcaag t 21

<210> 83

<211> 21

<212> DNA

<213> Artificial sequence9 <220>

<223> Artifical sequence

<400> 83 cctttaccat ggccgtgtag c 21

<210> 84

<211> 24

<212> DNA

<213> Artificial sequence10

<220>

<223> Artifical sequence

<400> 84 ccatgaatgg tgttcctcct tctc 24

<210> 85

<211> 20

<212> DNA

<213> Artificial sequence11

<220>

<223> Artifical sequence

<400> 85 aaacttgctg atgccgctgt 20

<210> 86

<211> 23

<212> DNA

<213> Artificial sequence12

<220>

<223> Artificial sequence

<400> 86 ccgcctttac cgataaccat acc 23

<210> 87

<211> 20

<212> DNA

<213> Artificial sequence13

<220>

<223> Artificial sequence

<400> 87 agagtttgat ymtggctcag 20

<210> 88

<211> 21

<212> DNA

<213> Artificial sequence14

<220>

<223> Artificial sequence <400> 88 acggytacct tgttacgact t 21

<210> 89

<211> 1833

<212> DNA

<213> Enterococcus avium ATCC 14025 2

<220>

<221 > misc_feature

<222> (11 ).. (12)

<223> n is a, c, g, or t

<400> 89 agagtttgat nntggctcag gacaaacgct ggcggcgtgc ctaatacatg caagtcgaac 60 gctttttctt tcaccgagct tgctccaccg aaagaaaagg agtggcgaac gggtgagtaa 120 cacgtgggta acctgcccat cagaagggga taacacttgg aaacaggtgc taataccgta 180 taacaataaa ccgcatggtt tcggtttgaa aggcgctttt gcgtcactga tggatggacc 240 cgcggtgcat tagctagttg gtgaggtaac ggctcaccaa ggcaacgatg catagccgac 300 ctgagagggt gatcggccac attgggactg agacacggcc caaactccta cgggaggcag 360 cagtagggaa tcttcggcaa tggacgcaag tctgaccgag caacgccgcg tgagtgaaga 420 aggttttcgg atcgtaaaac tctgttgtta gagaagaaca aggatgagag tagaatgttc 480 atccttgacg gtatctaacc agaaagcacg gctaactacg tgccagcagc cgcggtaata 540 cgtaggtggc aagcgttgtc cggatttatt gggcgtaaag cgagcgcagg cggtttctta 600 agtctgatgt gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggaaacttga 660 gtgcagaaga ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga 720 acaccagtgg cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg 780 gagcaaacag gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg 840 gagggtttcc gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg 900 accgcaaggt tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg 960 tttaattcga agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga 1020 gatagagctt ccccttcggg ggcaaagtga caggtggtgc atggttgtcg tcagctcgtg 1080 tcgtgagatg ttgggttaag tcccgcaacg agcgcaaccc ttattgttag ttgccatcat 1140 ttagttgggc acactagcga gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc 1200 aaatcatcat gccccttatg acctgggcta cacacgtgct acaatgggaa gtacaacgag 1260 tcgcgaagtc gcgaggctaa gctaatctct taaagcttct ctcagttcgg attgtaggct 1320 gcaactcgcc tacatgaagc cggaatcgct agtaatcgcg gatcagcacg ccgcggtgaa 1380 tacgttcccg ggccttgtac acaccgcccg tcacaccacg agagtttgta acacccgaag 1440 tcggtgaggt aaccttttgg agccagccgc ctaaggtggg atagatgatt ggggtgaagt 1500 cgtaacaagg tagccgtatc ggaaggtgcg gctggatcac ctcctttcta aggaaatagt 1560 tcatcgaaga agttacggaa actacacagg aatgctttat tcgttcagtt ttgagaggtc 1620 tactctcaaa tcgatccatg gatagcgggc tagcaaacat tttaaagcac cgtagacacg 1680 cagtagatga gtttttgata gtcagtgctt ttttgtgtat gaatggtgcc gaatggtaag 1740 acctgcgagc actagctcgt gtatggcccg ggagctgcct gggcgatgag acggataccc 1800 gtagctcgga cctagtactg tcgatcaatg cgc 1833

<210> 90

<211> 1546

<212> DNA

<213> Enterococcus avium 1

<400> 90 tgagtttgat cctggctcag gacgaacgct ggcggcgtgc ctaatacatg caagtcgaac 60 gctttttctt tcaccggagc ttgctccayc gaaagaaaag gagtggcgaa cgggtgagta 120 acacgtgggt aacctgccca tcagaagggg ataacacttg gaaacaggtg ctaataccgt 180 ataacaatcg aaaccgcatg gtttcggttt gaaaggcgct tttgcgtcac tgatggatgg 240 acccgcggtg cattagctag ttggtgaggt aacggctcac caaggcaacg atgcatagcc 300 gacctgagag ggtgatcggc cacattggga ctgagacacg gcccaaactc ctacgggagg 360 cagcagtagg gaatcttcgg caatggacgc aagtctgacc gagcaacgcc gcgtgagtga 420 agaaggtttt cggatcgtaa aactctgttg ttagagaaga acaaggatga gagtagaatg 480 ttcatccctt gacggtatct aaccagaaag ccacggctaa ctacgtgcca gcagccgcgg 540 taatacgtag gtggcaagcg ttgtccggat ttattgggcg taaagcgagc gcaggcggtt 600 tcttaagtct gatgtgaaag cccccggctc aaccggggag ggtcattgga aactgggaaa 660 cttgagtgca gaagaggaga gtggaattcc atgtgtagcg gtgaaatgcg tagatatatg 720 gaggaacacc agtggcgaag gcggctctct ggtctgtaac tgacgctgar gctcgaaagc 780 gtggggagca aacaggatta gataccctgg tagtccacgc cgtaaacgat gartgctaag 840 tgttggargg tttccgccct tcagtgctgc agctaacgca ttaagcactc cgcctgggga 900 rtacgaccgc aaggttgaaa ctcaaaggaa ttgacggggg cccgcacaag cggtggagca 960 tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt cttgacatcc tttgaccact 1020 ctagagatag agcttcccct tcgggggcaa agtgacaggt ggtgcatggt tgtcgtcagc 1080 tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccttatt gttagttgcc 1140 atcatttagt tgggcactct agcgagactg ccggtgacaa accggaggaa ggtggggatg 1200 acgtcaaatc atcatgcccc ttatgacctg ggctacacac gtgctacaat gggaagtaca 1260 acgagtcgcg aagtcgcgag gctaagctaa tctcttaaag cttctctcag ttcggattgt 1320 aggctgcaac tcgcctacat gaagccggaa tcgctagtaa tcgcggatca gcacgccgcg 1380 gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccacgagagt ttgtaacacc 1440 cgaagtcggt gaggtaacct tttggagcca gccgcctaag gtgggataga tgattggggt 1500 gaagtcgtaa caaggtagcc gtatcggaag gtgcggctgg atcacc 1546

<210> 91

<211> 1524

<212> DNA

<213> Enterococcus avium ATCC 14025 1

<400> 91 ccgtagagtt tgatcctggc tcaggacgaa cgctggcggc gtgcctaata catgcaagtc 60 gaacgctttt tctttcaccg gagcttgctc caccgaaaga aaaggagtgg cgaacgggtg 120 agtaacacgt gggtaacctg cccatcagaa ggggataaca cttggaaaca ggtgctaata 180 ccgtataaca atcgaaaccg catggtttcg gtttgaaagg cgcttttgcg tcactgatgg 240 atggacccgc ggtgcattag ctagttggtg aggtaacggc tcaccaaggc aacgatgcat 300 agccgacctg agagggtgat cggccacatt gggactgaga cacggcccaa actcctacgg 360 gaggcagcag tagggaatct tcggcaatgg acgcaagtct gaccgagcaa cgccgcgtga 420 gtgaagaagg ttttcggatc gtaaaactct gttgttagag aagaacaagg atgagagtag 480 aatgttcatc ccttgacggt atctaaccag aaagccacgg ctaactacgt gccagcagcc 540 gcggtaatac gtaggtggca agcgttgtcc ggatttattg ggcgtaaagc gagcgcaggc 600 ggtttcttaa gtctgatgtg aaagcccccg gctcaaccgg ggagggtcat tggaaactgg 660 gaaacttgag tgcagaagag gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata 720 tatggaggaa caccagtggc gaaggcggct ctctggtctg taactgacgc tgaggctcga 780 aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa cgatgagtgc 840 taagtgttgg agggtttccg cccttcagtg ctgcagctaa cgcattaagc actccgcctg 900 gggagtacga ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca caagcggtgg 960 agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac atcctttgac 1020 cactctagag atagagcttc cctgggggca aagtgacagg tggtgcatgg ttgtcgtcag 1080 ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccttat tgttagttgc 1140 catcatttag ttgggcactc tagcgagact gccggtgaca aaccggagga aggtggggat 1200 gacgtcaaat catcatgccc cttatgacct gggctacaca cgtgctacaa tgggaagtac 1260 aacgagtcgc gaagtcgcga ggctaagcta atctcttaaa gcttctctca gttcggattg 1320 taggctgcaa ctcgcctaca tgaagccgga atcgctagta atcgcggatc agcacgccgc 1380 ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac 1440 ccgaagtcgg tgaggtaacc ttttggagcc agccgcctaa ggtgggatag atgattgggg 1500 tgaagtcgta acaaggtagc cgtc 1524

<210> 92

<211> 1523

<212> DNA

<213> Enterococcus avium 2

<400> 92 cgctggcggc gtgcctaata catgcaagtc gaacgctttt tctttcaccg gagcttgctc 60 caccgaaaga aaaggagtgg cgaacgggtg agtaacacgt gggtaacctg cccatcagaa 120 ggggataaca cttggaaaca ggtgctaata ccgtataaca atcgaaaccg catggtttcg 180 gtttgaaagg cgcttttgcg tcactgatgg atggacccgc ggtgcattag ctagttggtg 240 aggtaacggc tcaccaaggc aacgatgcat agccgacctg agagggtgat cggccacatt 300 gggactgaga cacggcccaa actcctacgg gaggcagcag tagggaatct tcggcaatgg 360 acgcaagtct gaccgagcaa cgccgcgtga gtgaagaagg ttttcggatc gtaaaactct 420 gttgttagag aagaacaagg atgagagtag aatgttcatc ccttgacggt atctaaccag 480 aaagccacgg ctaactacgt gccagcagcc gcggtaatac gtaggtggca agcgttgtcc 540 ggatttattg ggcgtaaagc gagcgcaggc ggtttcttaa gtctgatgtg aaagcccccg 600 gctcaaccgg ggagggtcat tggaaactgg gaaacttgag tgcagaagag gagagtggaa 660 ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccagtggc gaaggcggct 720 ctctggtctg taactgacgc tgaggctcga aagcgtgggg agcaaacagg attagatacc 780 ctggtagtcc acgccgtaaa cgatgagtgc taagtgttgg agggtttccg cccttcagtg 840 ctgcagctaa cgcattaagc actccgcctg gggagtacga ccgcaaggtt gaaactcaaa 900 ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa 960 gaaccttacc aggtcttgac atcctttgac cactctagag atagagcttc cccttcgggg 1020 gcaaagtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt 1080 cccgcaacga gcgcaaccct tattgttagt tgccatcatt tagttgggca ctctagcgag 1140 actgccggtg acaaaccgga ggaaggtggg gatgacgtca aatcatcatg ccccttatga 1200 cctgggctac acacgtgcta caatgggaag tacaacgagt cgcgaagtcg cgaggctaag 1260 ctaatctctt aaagcttctc tcagttcgga ttgtaggctg caactcgcct acatgaagcc 1320 ggaatcgcta gtaatcgcgg atcagcacgc cgcggtgaat acgttcccgg gccttgtaca 1380 caccgcccgt cacaccacga gagtttgtaa cacccgaagt cggtgaggta accttttgga 1440 gccagccgcc taaggtggga tagatgattg gggtgaagtc gtaacaaggt agccgtatcg 1500 gaaggtgcgg ctggatcacc tcc 1523

<210> 93

<211> 1520

<212> DNA

<213> Prior Sequence 1

<400> 93 cgttggttta agcactctgg ctagagacgc tggcggcgtg cctaatacat gcaagtcgaa 60 cgctttttct ttcaccggag cttgctccac cgaaagaaaa ggagtggcga acgggtgagt 120 aacacgtggg taacctgccc atcagaaggg gataacactt ggaaacaggt gctaataccg 180 tataacaata gaaaccgcat ggtttctatt tgaaaggcgc ttttgcgtca ctgatggatg 240 gacccgcggt gcattagcta gttggtgagg taacggctca ccaaggcaac gatgcatagc 300 cgacctgaga gggtgatcgg ccacattggg actgagacac ggcccaaact cctacgggag 360 gcagcagtag ggaatcttcg gcaatggacg aaagtctgac cgagcaacgc cgcgtgagtg 420 aagaaggttt tcggatcgta aaactctgtt gttagagaag aacaaggatg agagtagaat 480 gttcatccct tgacggtatc taaccagaaa gccacggcta actacgtgcc agcagccgcg 540 gtaatacgta ggtggcaagc gttgtccgga tttattgggc gtaaagcgag cgcaggcggt 600 ttcttaagtc tgatgtgaaa gcccccggct caaccgggga gggtcattgg aaactgggaa 660 acttgagtgc agaagaggag agtggaattc catgtgtagc ggtgaaatgc gtagatatat 720 ggaggaacac cagtggcgaa ggcggctctc tggtctgtaa ctgacgctga ggctcgaaag 780 cgtggggagc aaacaggatt agataccctg gtagtccacg ccgtaaacga tgagtgctaa 840 gtgttggagg gtttccgccc ttcagtgctg cagctaacgc attaagcact ccgcctgggg 900 agtacgaccg caaggttgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc 960 atgtggttta attcgaagca acgcgaagaa ccttaccagg tcttgacatc ctttgaccac 1020 tctagagata gagcttcccc ttcgggggca aagtgacagg tggtgcatgg ttgtcgtcag 1080 ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccttat tgttagttgc 1140 catcatttag ttgggcactc cagcgagact gccggtgaca aaccggagga aggtggggat 1200 gacgtcaaat catcatgccc cttatgacct gggccacaca cgtgctacaa tgggaagtac 1260 aacgagtcgc gaagtcgcga ggctaagcta atctcttaaa gcttctctca gttcggattg 1320 taggctgcaa ctcgcctaca tgaagccgga atcgctagta atcgcggatc agcacgccgc 1380 ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac 1440 ccgaagtcgg cgaggtaacc ttttggagcc agccgcctaa ggtgggatag atgatggggg 1500 aagtcgagaa agtcgttgaa 1520 <210> 94

<211> 1513

<212> DNA

<213> Prior Sequence 2

<400> 94 cttggagtct gctaggagac gctggcggcg tgcctaatac atgcaagtcg aacgcttttt 60 ctttcaccgg agcttgctcc accgaaagaa aaggagtggc gaacgggtga gtaacacgtg 120 ggtaacctgc ccatcagaag gggataacac ttggaaacag gtgctaatac cgtataacaa 180 tagaaaccgc atggtttcta tttgaaaggc gcttttgcgt cactgatgga tggacccgcg 240 gtgcattagc tagttggtga ggtaacggct caccaaggca acgatgcata gccgacctga 300 gagggtgatc ggccacattg ggactgagac acggcccaaa ctcctacggg aggcagcagt 360 agggaatctt cggcaatgga cgaaagtctg accgagcaac gccgcgtgag tgaagaaggt 420 tttcggatcg taaaactctg ttgttagaga agaacaagga tgagagtaga atgttcatcc 480 cttgacggta tctaaccaga aagccacggc taactacgtg ccagcagccg cggtaatacg 540 taggtggcaa gcgttgtccg gatttattgg gcgtaaagcg agcgcaggcg gtttcttaag 600 tctgatgtga aagcccccgg ctcaaccggg gagggtcatt ggaaactggg aaacttgagt 660 gcagaagagg agagtggaat tccatgtgta gcggtgaaat gcgtagatat atggaggaac 720 accagtggcg aaggcggctc tctggtctgt aactgacgct gaggctcgaa agcgtgggga 780 gcaaacagga ttagataccc tggtagtcca cgccgtaaac gatgagtgct aagtgttgga 840 gggtttccgc ccttcagtgc tgcagctaac gcattaagca ctccgcctgg ggagtacgac 900 cgcaaggttg aaactcaaag gaattgacgg gggcccgcac aagcggtgga gcatgtggtt 960 taattcgaag caacgcgaag aaccttacca ggtcttgaca tcctttgacc actctagaga 1020 tagagcttcc ccttcggggg caaagtgaca ggtggtgcat ggttgtcgtc agctcgtgtc 1080 gtgagatgtt gggttaagtc ccgcaacgag cgcaaccctt attgttagtt gccatcattt 1140 agttgggcac tctagcgaga ctgccggtga caaaccggag gaaggtgggg atgacgtcaa 1200 atcatcatgc cccttatgac ctgggctaca cacgtgctac aatgggaagt acaacgagtc 1260 gcgaagtcgc gaggctaagc taatctctta aagcttctct cagttcggat tgtaggctgc 1320 aactcgccta catgaagccg gaatcgctag taatcgcgga tcagcacgcc gcggtgaata 1380 cgttcccggg ccttgtacac accgcccgtc acaccacgag agtttgtaac acccgaagtc 1440 ggtgaggtaa ccttttggag ccagccgcct aaggtgggat agatgatggg ggaagtctat 1500 caataccgaa tgc 1513

<210> 95

<211> 1507

<212> DNA <213> Prior Sequence 3

<400> 95 atcagtactg ctagagacgc tggcggcgtg cctaatacat gcaagtcgaa cgctttttct 60 ttcaccggag cttgctccac cgaaagaaaa ggagtggcga acgggtgagt aacacgtggg 120 taacctgccc atcagaaggg gataacactt ggaaacaggt gctaataccg tataacaata 180 gaagccgcat ggtttctatt tgaaaggcgc ttttgcgtca ctgatggatg gacccgcggt 240 gcattagcta gttggtgagg taacggctca ccaaggcaac gatgcatagc cgacctgaga 300 gggtgatcgg ccacattggg actgagacac ggcccaaact cctacgggag gcagcagtag 360 ggaatcttcg gcaatggacg aaagtctgac cgagcaacgc cgcgtgagtg aagaaggttt 420 tcggatcgta aaactctgtt gtcagagaag aacaaggatg agagtagaat gttcatccct 480 tgacggtatc taaccagaaa gccacggcta actacgtgcc agcagccgcg gtaatacgta 540 ggtggcaagc gttgtccgga tttattgggc gtaaagcgag cgcaggcggt ttcttaagtc 600 tgatgtgaaa gcccccggct caaccgggga gggtcattgg aaactgggaa acttgagtgc 660 agaagaggag agtggaattc catgtgtagc ggtgaaatgc gtagatatat ggaggaacac 720 cagtggcgaa ggcggccctc tggtctgtaa ctgacgctga ggctcgaaag cgtggggagc 780 aaacaggatt agataccctg gtagtccacg ccgtaaacga tgagtgctaa gtgttggagg 840 gtttccgccc ttcagtgctg cagctaacgc attaagcact ccgcctgggg agtacgaccg 900 caaggttgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta 960 attcgaagca acgcgaagaa ccttaccagg tcttgacatc ctttgaccac tctagagata 1020 gagcttcccc ttcgggggca aagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt 1080 gagatgttgg gttaagtccc gcaacgagcg caacccttat tgttagttgc catcatttag 1140 ttgggcactc tagcgagact gccggtgaca aaccggagga aggtggggat gacgtcaaat 1200 catcatgccc cttatgacct gggctacaca cgtgctacaa tgggaagtac aacgagtcgc 1260 gaagtcgcga ggctaagcta atctcttaaa gcttctctca gttcggattg taggctgcaa 1320 ctcgcctaca tgaagccgga atcgctagta atcgcggatc agcacgccgc ggtgaatacg 1380 ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac ccgaagtcgg 1440 tgaggtaacc ttttggagcc agccgcctaa ggtgggatag atgatggggg aagtcataag 1500 accgatc 1507

<210> 96

<211> 1500

<212> DNA

<213> Enterococcus malodoratus

<400> 96 ggacgaacgc tggcggcgtg cctaatacat gcaagtcgaa cgctttttct ttcaccggag 60 cttgctccac cgaaagaaaa ggagtggcga acgggtgagt aacacgtggg taacctgccc 120 atcagaaggg gataacactt ggaaacaggt gctaataccg tataacagta gaaaccgcat 180 ggtttctatt tgaaaggcgc ttttgcgtca ctgatggatg gacccgcggt gcattagcta 240 gttggtgagg taacggctca ccaaggcaac gatgcatagc cgacctgaga gggtgatcgg 300 ccacattggg actgagacac ggcccaaact cctacgggag gcagcagtag ggaatcttcg 360 gcaatggacg aaagtctgac cgagcaacgc cgcgtgagtg aagaaggttt tcggatcgta 420 aaactctgtt gttagagaag aacaaggatg agagtaaaac gttcatccct tgacggtatc 480 taaccagaaa gccacggcta actacgtgcc agcagccgcg gtaatacgta ggtggcaagc 540 gttgtccgga tttattgggc gtaaagcgag cgcaggcggt ttcttaagtc tgatgtgaaa 600 gcccccggct caaccgggga gggtcattgg aaactgggaa acttgagtgc agaagaggag 660 agtggaattc catgtgtagc ggtgaaatgc gtagatatat ggaggaacac cagtggcgaa 720 ggcggctctc tggtctgtaa ctgacgctga ggctcgaaag cgtggggagc aaacaggatt 780 agataccctg gtagtccacg ccgtaaacga tgagtgctaa gtgttggagg gtttccgccc 840 ttcagtgctg cagctaacgc attaagcact ccgcctgggg agtacgaccg caaggttgaa 900 actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca 960 acgcgaagaa ccttaccagg tcttgacatc ctttgaccac tctagagata gagcttcccc 1020 ttcgggggca aagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg 1080 gttaagtccc gcaacgagcg caacccttat tgttagttgc catcatttag ttgggcactc 1140 tagcgagact gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc 1200 cttatgacct gggctacaca cgtgctacaa tgggaagtac aacgagtcgc gaagtcgcga 1260 ggctaagcta atctcttaaa gcttctctca gttcggattg taggctgcaa ctcgcctaca 1320 tgaagccgga atcgctagta atcgcggatc agcacgccgc ggtgaatacg ttcccgggcc 1380 ttgtacacac cgcccgtcac accacgagag tttgtaacac ccgaagtcgg tgaggtaacc 1440 ttttggagcc agccgcctaa ggtgggatag atgattgggg tgaagtcgta acaaggtagc 1500

<210> 97

<211> 1496

<212> DNA

<213> Enterococcus raffinosus

<400> 97 gacgaacgct ggcggcgtgc ctaatacatg caagtcgaac gccttttctt tcaccggagc 60 ttgctccacc gaaagaaaag gagtggcgaa cgggtgagta acacgtgggt aacctgccca 120 tcagaagggg ataacacttg gaaacaggtg ctaataccgt ataacaatag aaaccgcatg 180 gtttctattt gaaaggcgct tttgcgtcac tgrtggatgg acccgcggtg cattagctag 240 ttggtgaggt aacggctcac caaggcaacg atgcatagcc gacctgagag ggtgatcggc 300 cacattggga ctgagacacg gcccaaactc ctacgggagg cagcagtagg gaatcttcgg 360 caatggacga aagtctgacc gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa 420 aactctgttg ttagagaaga acaaggatga gagtagaatg ttcatccctt gacggtatct 480 aaccagaaag ccacggctaa ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg 540 ttgtccggat ttattgggcg taaagcgagc gcaggcggtt tcttaagtct gatgtgaaag 600 cccccggctc aaccggggag ggtcattgga aactgggaaa cttgagtgca gaagaggaga 660 gtggaattcc atgtgtagcg gtgaaatgcg tagatatatg gaggaacacc agtggcgaag 720 gcggctctct ggtctgtaac tgacgctgag gctcgaaagc gtggggagca aacaggatta 780 gataccctgg tagtccacgc cgtaaacgat gagtgctaag tgttggaggg tttccgccct 840 tcagtgctgc agctaacgca ttaagcactc cgcctgggga gtacgaccgc aaggttgaaa 900 ctcaaaggaa ttgacggggg cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa 960 cgcgaagaac cttaccaggt cttgacatcc tttgaccact ctagagatag agcttcccct 1020 tcgggggcaa agtgacaggt ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg 1080 ttaagtcccg caacgagcgc aacccttatt gttagttgcc atcatttagt tgggcactct 1140 agcgagactg ccggtgacaa accggaggaa ggtggggatg acgtcaaatc atcatgcccc 1200 ttatgacctg ggctacacac gtgctacaat gggaagtaca acgagtcgcg aagtcgcgag 1260 gctaagctaa tctcttaaag cttctctcag ttcggattgt aggctgcaac tcgcctacat 1320 gaagccggaa tcgctagtaa tcgcggatca gcacgccgcg gtgaatacgt tcccgggcct 1380 tgtacacacc gcccgtcaca ccacgagagt ttgtaacacc cgaagtcggt gaggtaacct 1440 tttggagcca gccgcctaag gtgggataga tgattggggt gaagtcgtaa caaggt 1496

<210> 98

<211> 1481

<212> DNA

<213> Prior Sequence 4

<400> 98 gacgaacgct ggcggcgtgc ctaatacatg caagtcgaac gctttttctt tcaccggagc 60 ttgctccacc gaaagaaaag gagtggcgaa cgggtgagta acacgtgggt aacctgccca 120 tcagaagggg ataacacttg gaaacaggtg ctaataccgt ataacagtcg aaaccgcatg 180 gtttcgattt gaaaggcgct tttgcgtcac tgatggatgg acccgcggtg cattagctag 240 ttggtgaggt gacggctcac caaggcaacg atgcatagcc gacctgagag ggtgatcggc 300 cacattggga ctgagacacg gcccaaactc ctacgggagg cagcagtagg gaatcttcgg 360 caatggacga aagtctgacc gagcaacgcc gcgtgggtga agaaggtttt cggatcgtaa 420 aactctgttg ttagagaaga acaaggatga gagtagaatg ttcatccctt gacggtatct 480 aaccagaaag ccacggctaa ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg 540 ttgtccggat ttattgggcg taaagcgagc gcaggcggtt tcttaagtct gatgtgaaag 600 cccccggctc aaccggggag ggtcattgga aactgggaaa cttgagtgca gaagaggaga 660 gtggaattcc atgtgtagcg gtgaaatgcg tagatatatg gaggaacacc agtggcgaag 720 gcggctctct ggtctgtaac tgacgctgag gctcgaaagc gtggggagca aacaggatta 780 gataccctgg tagtccacgc cgtaaacgat gagtgctaag tgttggaggg tttccgccct 840 tcagtgctgc agctaacgca ttaagcactc cgcctgggga gtacgaccgc aaggttgaaa 900 ctcaaaggaa ttgacggggg cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa 960 cgcgaagaac cttaccaggt cttgacatcc tttgaccact ctagagatag agcttcccct 1020 tcgggggcaa agtgacaggt ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg 1080 ttaagtcccg caacgagcgc aacccttatt gttagttgcc atcatttagt tgggcactct 1140 agcgagactg ccggtgacaa accggaggaa ggtggggatg acgtcaaatc atcatgcccc 1200 ttatgacctg ggctacacac gtgctacaat gggaagtaca acgagtcgcg aagtcgcgag 1260 gctaagctaa tctcttaaag cttctctcag ttcggattgt aggctgcaac tcgcctacat 1320 gaagccggaa tcgctagtaa tcgcggatca gcacgccgcg gtgaatacgt tcccgggcct 1380 tgtacacacc gcccgtcaca ccacgagagt ttgtaacacc cgaagtcggt gaggtaacct 1440 tttggagcca gccgcctaag gtgggataga tgattggggt g 1481

<210> 99

<211> 1461

<212> DNA

<213> Enterococcus malodoratus 2

<400> 99 acgaacgctg gcggcgtgcc taatacatgc aagtcgaacg ctttttcttt caccggagct 60 tgctccaccg aaagaaaagg agtggcgaac gggtgagtaa cacgtgggta acctgcccat 120 cagaagggga taacacttgg aaacaggtgc taataccgta taacagtaga aaccgcatgg 180 tttctatttg aaaggcgctt ttgcgtcact gatggatgga cccgcggtgc attagctagt 240 tggtgaggta acggctcacc aaggcaacga tgcatagccg acctgagagg gtgatcggcc 300 acattgggac tgagacacgg cccaaactcc tacgggaggc agcagtaggg aatcttcggc 360 aatggacgaa agtctgaccg agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa 420 actctgttgt tagagaagaa caaggatgag agtaaaacgt tcatcccttg acggtatcta 480 accagaaagc cacggctaac tacgtgccag cagccgcggt aatacgtagg tggcaagcgt 540 tgtccggatt tattgggcgt aaagcgagcg caggcggttt cttaagtctg atgtgaaagc 600 ccccggctca accggggagg gtcattggaa actgggaaac ttgagtgcag aagaggagag 660 tggaattcca tgtgtagcgg tgaaatgcgt agatatatgg aggaacacca gtggcgaagg 720 cggctctctg gtctgtaact gacgctgagg ctcgaaagcg tggggagcaa acaggattag 780 ataccctggt agtccacgcc gtaaacgatg agtgctaagt gttggagggt ttccgccctt 840 cagtgctgca gctaacgcat taagcactcc gcctggggag tacgaccgca aggttgaaac 900 tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac 960 gcgaagaacc ttaccaggtc ttgacatcct ttgaccactc tagagataga gcttcccctt 1020 cgggggcaaa gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt 1080 taagtcccgc aacgagcgca acccttattg ttagttgcca tcatttagtt gggcactcta 1140 gcgagactgc cggtgacaaa ccggaggaag gtggggatga cgtcaaatca tcatgcccct 1200 tatgacctgg gctacacacg tgctacaatg ggaagtacaa cgagtcgcga agtcgcgagg 1260 ctaagctaat ctcttaaagc ttctctcagt tcggattgta ggctgcaact cgcctacatg 1320 aagccggaat cgctagtaat cgcggatcag cacgccgcgg tgaatacgtt cccgggcctt 1380 gtacacaccg cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg aggtaacctt 1440 ttggagccag ccgcctaagg t 1461

<210> 100

<211> 1453

<212> DNA

<213> Enterococcus avium 3

<400> 100 catgcaagtc gaacgctttt tctttcaccg gagcttgctc caccgaaaga aaaggagtgg 60 cgaacgggtg agtaacacgt gggtaacctg cccatcagaa ggggataaca cttggaaaca 120 ggtgctaata ccgtataaca atcgaaaccg catggtttcg gtttgaaagg cgcttttgcg 180 tcactggtgg atggacccgc ggtgcattag ctagttggtg aggtaacggc tcaccaaggc 240 aacgatgcat agccgacctg agagggtgat cggccacatt gggactgaga cacggcccaa 300 actcctacgg gaggcagcag tagggaatct tcggcaatgg acgcaagtct gaccgagcaa 360 cgccgcgtga gtgaagaagg ttttcggatc gtaaaactct gttgttagag aagaacaagg 420 atgagagtaa aatgttcatc ccttgacggt atctaaccag aaagccacgg ctaactacgt 480 gccagcagcc gcggtaatac gtaggtggca agcgttgtcc ggatttattg ggcgtaaagc 540 gagcgcaggc ggtttcttaa gtctgatgtg aaagcccccg gctcaaccgg ggagggtcat 600 tggaaactgg gaaacttgag tgcagaagag gagagtggaa ttccatgtgt agcggtgaaa 660 tgcgtagata tatggaggaa caccagtggc gaaggcggct ctctggtctg taactgacgc 720 tgaggctcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa 780 cgatgagtgc taagtgttgg agggtttccg cccttcagtg ctgcagctaa cgcattaagc 840 actccgcctg gggagtacga ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca 900 caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac 960 atcctttgac cactctagag atagagcttc cccttcgggg gcaaagtgac aggtggtgca 1020 tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct 1080 tattgttagt tgccatcatt tagttgggca ctctagcgag actgccggtg acaaaccgga 1140 ggaaggtggg gatgacgtca aatcatcatg ccccttatga cctgggctac acacgtgcta 1200 caatgggaag tacaacgagt cgcgaagtcg cgaggctaag ctaatctctt aaagcttctc 1260 tcagttcgga ttgtaggctg caactcgcct acatgaagcc ggaatcgcta gtaatcgcgg 1320 atcagcacgc cgcggtgaat acgttcccgg gccttgtaca caccgcccgt cacaccacga 1380 gagtttgtaa cacccgaagt cggtgaggta accttttgga gccagccgcc taaggtggga 1440 tagatgattg ggg 1453

<210> 101

<211> 1429

<212> DNA

<213> Enterococcus avium ATCC 14025 3

<400> 101 acgctggcgg cgtgcctaat acatgcaagt cgaacgcttt ttctttcacc ggagcttgct 60 ccaccgaaag aaaaggagtg gcgaacgggt gagtaacacg tgggtaacct gcccatcaga 120 aggggataac acttggaaac aggtgctaat accgtataac aatcgaaacc gcatggtttc 180 ggtttgaaag gcgcttttgc gtgactgatg gatggacccg cggtgcatta gctagttggt 240 gaggtaacgg ctcaccaagg caacgatgca tagccgacct gagagggtga tcggccacat 300 tgggactgag acacggccca aactcctacg ggaggcagca gtagggaatc ttcggcaatg 360 gacgcaagtc tgaccgagca acgccgcgtg agtgaagaag gttttcggat cgtaaaactc 420 tgttgttaga gaagaacaag gatgagagta gaatgttcat cccttgacgg tatctaacca 480 gaaagccacg gctaactacg tgccagcagc cgcggtaata cgtaggtggc aagcgttgtc 540 cggatttatt gggcgtaaag cgagcgcagg cggtttctta agtctgatgt gaaagccccc 600 ggctcaaccg gggagggtca ttggaaactg ggaaacttga gtgcagaaga ggagagtgga 660 attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg cgaaggcggc 720 tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag gattagatac 780 cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc gcccttcagt 840 gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt tgaaactcaa 900 aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga agcaacgcga 960 agaaccttac caggtcttga catcctttga ccactctaga gatagagctt ccccttcggg 1020 ggcaaagtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag 1080 tcccgcaacg agcgcaaccc ttattgttag ttgccatcat ttagttgggc actctagcga 1140 gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat gccccttatg 1200 acctgggcta cacacgtgct acaatgggaa gtacaacgag tcgcgaagtc gcgaggctaa 1260 gctaatctct taaagcttct ctcagttcgg attgtaggct gcaactcgcc tacatgaagc 1320 cggaatcgct agtaatcgcg gatcagcacg ccgcggtgaa tacgttcccg ggccttgtac 1380 acaccgcccg tcacaccacg agagtttgta acacccgaag tcggtgagg 1429

<210> 102

<211> 1425

<212> DNA

<213> Enterococcus raffinosus 2

<400> 102 gctggcggcg tgcctaatac atgcaagtcg aacgcctttt ctttcaccgg agcttgctcc 60 accgaaagaa aaggagtggc gaacgggtga gtaacacgtg ggtaacctgc ccatcagaag 120 gggataacac ttggaaacag gtgctaatac cgtataacaa tagaaaccgc atggtttcta 180 tttgaaaggc gcttttgcgt cactgatgga tggacccgcg gtgcattagc tagttggtga 240 ggtaacggct caccaaggca acgatgcata gccgacctga gagggtgatc ggccacattg 300 ggactgagac acggcccaaa ctcctacggg aggcagcagt agggaatctt cggcaatgga 360 cgaaagtctg accgagcaac gccgcgtgag tgaagaaggt tttcggatcg taaaactctg 420 ttgttagaga agaacaagga tgagagtaga atgttcatcc cttgacggta tctaaccaga 480 aagccacggc taactacgtg ccagcagccg cggtaatacg taggtggcaa gcgttgtccg 540 gatttattgg gcgtaaagcg agcgcaggcg gtttcttaag tctgatgtga aagcccccgg 600 ctcaaccggg gagggtcatt ggaaactggg aaacttgagt gcagaagagg agagtggaat 660 tccatgtgta gcggtgaaat gcgtagatat atggaggaac accagtggcg aaggcggctc 720 tctggtctgt aactgacgct gaggctcgaa agcgtgggga gcaaacagga ttagataccc 780 tggtagtcca cgccgtaaac gatgagtgct aagtgttgga gggtttccgc ccttcagtgc 840 tgcagctaac gcattaagca ctccgcctgg ggagtacgac cgcaaggttg aaactcaaag 900 gaattgacgg gggcccgcac aagcggtgga gcatgtggtt taattcgaag caacgcgaag 960 aaccttacca ggtcttgaca tcctttgacc actctagaga tagagcttcc ccttcggggg 1020 caaagtgaca ggtggtgcat ggttgtcgtc agctcgtgtc gtgagatgtt gggttaagtc 1080 ccgcaacgag cgcaaccctt attgttagtt gccatcattt agttgggcac tctagcgaga 1140 ctgccggtga caaaccggag gaaggtgggg atgacgtcaa atcatcatgc cccttatgac 1200 ctgggctaca cacgtgctac aatgggaagt acaacgagtc gcgaagtcgc gaggctaagc 1260 taatctctta aagcttctct cagttcggat tgtaggctgc aactcgccta catgaagccg 1320 gaatcgctag taatcgcgga tcagcacgcc gcggtgaata cgttcccggg ccttgtacac 1380 accgcccgtc acaccacgag agtttgtaac acccgaagtc ggtga 1425

<210> 103

<211> 1421

<212> DNA

<213> Enterococcus gilvus

<400> 103 gagcttgctc caccgaaaga aaaggagtgg cgaacgggtg agtaacacgt gggtaacctg 60 cccatcagaa ggggataaca cttggaaaca ggtgctaata ccgtataaca atagaaaccg 120 catggtttct atttgaaagg cgcttttgcg tcactgatgg atggacccgc ggtgcattag 180 ctagttggtg aggtaacggc tcaccaaggc aacgatgcat agccgacctg agagggtgat 240 cggccacatt gggactgaga cacggcccaa actcctacgg gaggcagcag tagggaatct 300 tcggcaatgg acgaaagtct gaccgagcaa cgccgcgtga gtgaagaagg ttttcggatc 360 gtaaaactct gttgttagag aagaacaagg atgagagtaa aatgttcatc ccttgacggt 420 atctaaccag aaagccacgg ctaactacgt gccagcagcc gcggtaatac gtaggtggca 480 agcgttgtcc ggatttattg ggcgtaaagc gagcgcaggc ggtttcttaa gtctgatgtg 540 aaagcccccg gctcaaccgg ggagggtcat tggaaactgg gaaacttgag tgcagaagag 600 gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata tatggaggaa caccagtggc 660 gaaggcggct ctctggtctg taactgacgc tgaggctcga aagcgtgggg agcaaacagg 720 attagatacc ctggtagtcc acgccgtaaa cgatgagtgc taagtgttgg agggtttccg 780 cccttcagtg ctgcagctaa cgcattaagc actccgcctg gggagtacga ccgcaaggtt 840 gaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgaa 900 gcaacgcgaa gaaccttacc aggtcttgac atcctttgac cactctagag atagagcttc 960 cccttcgggg gcaaagtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt 1020 tgggttaagt cccgcaacga gcgcaaccct tattgttagt tgccatcatt tagttgggca 1080 ctctagcgag actgccggtg acaaaccgga ggaaggtggg gatgacgtca aatcatcatg 1140 ccccttatga cctgggctac acacgtgcta caatgggaag tacaacgagt cgcgaagtcg 1200 cgaggctaag ctaatctctt aaagcttctc tcagttcgga ttgtaggctg caactcgcct 1260 acatgaagcc ggaatcgcta gtaatcgcgg atcagcacgc cgcggtgaat acgttcccgg 1320 gccttgtaca caccgcccgt cacaccacga gagtttgtaa cacccgaagt cggtgaggta 1380 accttttgga gccagccgcc taaggtggga tagatgattg g 1421 <210> 104

<211> 1472

<212> DNA

<213> Prior Sequence 5

<400> 104 acgctggcgg cgtgcctata catgcaagtc gaacgcttct ttttccaccg gagcttgctc 60 caccggaaaa agaggagtgg cgaacgggtg agtaacacgt gggtaacctg cccatcagaa 120 ggggataaca cttggaaaca ggtgctaata ccgtataaca atcgaaaccg catggttttg 180 atttgaaagg cgctttcggg tgtcgctgat ggatggaccc gcggtgcatt agctagttgg 240 tgaggtaacg gctcaccaag gcgacgatgc atagccgacc tgagagggtg atcggccaca 300 ttgggactga gacacggccc aaactcctac gggaggcagc agtagggaat cttcggcaat 360 ggacgaaagt ctgaccgagc aacgccgcgt gagtgaagaa ggttttcgga tcgtaaaact 420 ctgttgttag agaagaacaa ggatgagagt aactgttcat cccttgacgg tatctaacca 480 gaaagccacg gctaactacg tgccagcagc cgcggtaata cgtaggtggc aagcgttgtc 540 cggatttatt gggcgtaaag cgagcgcagg cggtttctta agtctgatgt gaaagccccc 600 ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga ggagagtgga 660 attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg cgaaggcggc 720 tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag gattagatac 780 cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc gcccttcagt 840 gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt tgaaactcaa 900 aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga agcaacgcga 960 agaaccttac caggtcttga catcctttga ccactctaga gatagagctt ccccttcggg 1020 ggcaaagtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag 1080 tcccgcaacg agcgcaaccc ttattgttag ttgccatcat ttagttgggc actctagcaa 1140 gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat gccccttatg 1200 acctgggcta cacacgtgct acaatgggaa gtacaacgag tcgcaaagtc gcgaggctaa 1260 gctaatctct taaagcttct ctcagttcgg attgtaggct gcaactcgcc tacatgaagc 1320 cggaatcgct agtaatcgcg gatcagcacg ccgcggtgaa tacgttcccg ggccttgtac 1380 acaccgcccg tcacaccacg agagtttgta acacccgaag tcggtgaggt aaccttttgg 1440 agccagccgc ctaaggtgga tagagactgg gg 1472

<210> 105

<211> 1469

<212> DNA

<213> Prior Sequence 6 <400> 105 cgcgagctgc ggcgtcctat acatgcaagt cgaacgcttc tttttccacc ggagcttgct 60 ccaccggaaa aagaggagtg gcgaacgggt gagtaacacg tgggtaacct gcccatcaga 120 aggggataac acttggaaac aggtgctaat accgtataac aatcgaaacc gcatggtttt 180 gatttgaaag gcgctttcgg gtgtcgctga tggatggacc cgcggtgcat tagctagttg 240 gtgaggtaac ggctcaccaa ggcgacgatg catagccgac ctgagagggt gatcggccac 300 attgggactg agacacggcc caaactccta cgggaggcag cagtagggaa tcttcggcaa 360 tggacgaaag tctgaccgag caacgccgcg tgagtgaaga aggttttcgg atcgtaaaac 420 tctgttgtta gagaagaaca aggatgagag taactgttca tcccttgacg gtatctaacc 480 agaaagccac ggctaactac gtgccagcag ccgcggtaat acgtaggtgg caagcgttgt 540 ccggatttat tgggcgtaaa gcgagcgcag gcggtttctt aagtctgatg tgaaagcccc 600 cggctcaacc ggggagggtc attggaaact gggagacttg agtgcagaag aggagagtgg 660 aattccatgt gtagcggtga aatgcgtaga tatatggagg aacaccagtg gcgaaggcgg 720 ctctctggtc tgtaactgac gctgaggctc gaaagcgtgg ggagcaaaca ggattagata 780 ccctggtagt ccacgccgta aacgatgagt gctaagtgtt ggagggtttc cgcccttcag 840 tgctgcagct aacgcattaa gcactccgcc tggggagtac gaccgcaagg ttgaaactca 900 aaggaattga cgggggcccg cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg 960 aagaacctta ccaggtcttg acatcctttg accactctag agatagagct tccccttcgg 1020 gggcaaagtg acaggtggtg catggttgtc gtcagctcgt gtcgtgagat gttgggttaa 1080 gtcccgcaac gagcgcaacc cttattgtta gttgccatca tttagttggg cactctagca 1140 agactgccgg tgacaaaccg gaggaaggtg gggatgacgt caaatcatca tgccccttat 1200 gacctgggct acacacgtgc tacaatggga agtacaacga gtcgcaaagt cgcgaggcta 1260 agctaatctc ttaaagcttc tctcagttcg gattgtaggc tgcaactcgc ctacatgaag 1320 ccggaatcgc tagtaatcgc ggatcagcac gccgcggtga atacgttccc gggccttgta 1380 cacaccgccc gtcacaccac gagagtttgt aacacccgaa gtcggtgagg taaccttttg 1440 gagccagccg cctaaggtgg aagatatgg 1469

<210> 106

<211> 1507

<212> DNA

<213> Prior Sequence 7

<400> 106 cgaacgctgg cggcgtgcct aatacatgca agtcgaacgc ttctttttcc accggagctt 60 gctccaccgg aaaaagagga gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc 120 agaaggggat aacacttgga aacaggtgct aataccgtat aacaatcgaa accgcatggt 180 tttgatttga aaggcgcttt cgggtgtcgc tgatggatgg acccgcggtg cattagctag 240 ttggtgaggt aacggctcac caaggcgacg atgcatagcc gacctgagag ggtgatcggc 300 cacattggga ctgagacacg gcccaaactc ctacgggagg cagcagtagg gaatcttcgg 360 caatggacga aagtctgacc gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa 420 aactctgttg ttagagaaga acaaggatga gagtaactgt tcatcccttg acggtatcta 480 accagaaagc cacggctaac tacgtgccag cagccgcggt aatacgtagg tggcaagcgt 540 tgtccggatt tattgggcgt aaagcgagcg caggcggttt cttaagtctg atgtgaaagc 600 ccccggctca accggggagg gtcattggaa actgggagac ttgagtgcag aagaggagag 660 tggaattcca tgtgtagcgg tgaaatgcgt agatatatgg aggaacacca gtggcgaagg 720 cggctctctg gtctgtaact gacgctgagg ctcgaaagcg tggggagcaa acaggattag 780 ataccctggt agtccacgcc gtaaacgatg agtgctaagt gttggagggt ttccgccctt 840 cagtgctgca gctaacgcat taagcactcc gcctggggag tacgaccgca aggttgaaac 900 tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac 960 gcgaagaacc ttaccaggtc ttgacatcct ttgaccactc tagagataga gcttcccctt 1020 cgggggcaaa gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt 1080 taagtcccgc aacgagcgca acccttattg ttagttgcca tcatttagtt gggcactcta 1140 gcaagactgc cggtgacaaa ccggaggaag gtggggatga cgtcaaatca tcatgcccct 1200 tatgacctgg gctacacacg tgctacaatg ggaagtacaa cgagtcgcaa agtcgcgagg 1260 ctaagctaat ctcttaaagc ttctctcagt tcggattgta ggctgcaact cgcctacatg 1320 aagccggaat cgctagtaat cgcggatcag cacgccgcgg tgaatacgtt cccgggcctt 1380 gtacacaccg cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg aggtaacctt 1440 ttggagccag ccgcctaagg tgggatagat gattggggtg aagtcgtaac aaggtagccg 1500 tatcgga 1507

<210> 107

<211> 1337

<212> DNA

<213> Prior Sequence 8

<400> 107 gctttttctt tcaccggagc ttgctccacc gaaagaaaaa gagtggcgaa cgggtgagta 60 acacgtgggt aacctgccca tcagaagggg ataacacttg gaaacaggtg ctaataccgt 120 ataacaagta aaaccgcatg gttttacttt gaaaggcgct tttgcgtcac tgatggatgg 180 acccgcggtg cattagctag ttggtgaggt aacggctcac caaggcaacg atgcatagcc 240 gacctgagag ggtgatcggc cacattggga ctgagacacg gcccaaactc ctacgggagg 300 cagcagtagg gaatcttcgg caatggacga aagtctgacc gagcaacgcc gcgtgagtga 360 agaaggtttt cggatcgtaa aactctgttg ttagagaaga acaaggatga gagtaaaatg 420 ttcatccctt gacggtatct aaccagaaag ccacggctaa ctacgtgcca gcagccgcgg 480 taatacgtag gtggcaagcg ttgtccggat ttattgggcg taaagcgagc gcaggcggtt 540 tcttaagtct gatgtgaaag cccccggctc aaccggggag ggtcattgga aactgggaaa 600 cttgagtgca gaagaggaga gtggaattcc atgtgtagcg gtgaaatgcg tagatatatg 660 gaggaacacc agtggcgaag gcggctctct ggtctgtaac tgacgctgag gctcgaaagc 720 gtggggagca aacaggatta gataccctgg tagtccacgc cgtaaacgat gagtgctaag 780 tgttggaggg tttccgccct tcagtgctgc agctaacgca ttaagcactc cgcctgggga 840 gtacgaccgc aaggttgaaa ctcaaaggaa ttgacggggg cccgcacaag cggtggagca 900 tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt cttgacatcc tttgaccact 960 ctagagatag agcttcccct tcgggggcaa agtgacaggt ggtgcatggt tgtcgtcagc 1020 tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccttatt gttagttgcc 1080 atcatttagt tgggcactct agcgagactg ccggtgacaa accggaggaa ggtggggatg 1140 acgtcaaatc atcatgcccc ttatgacctg ggctacacac gtgctacaat gggaagtaca 1200 acgagtcgcg aagtcgcgag gctaagctaa tctcttaaag cttctctcag ttcggattgt 1260 aggctgcaac tcgcctacat gaagccggaa tcgctagtaa tcgcggatca gcacgccgcg 1320 gtgaatacgt tcccggg 1337

<210> 108

<211> 1344

<212> DNA

<213> Prior Sequence 9

<400> 108 gtcgaacgct ttttctttca ccggagcttg ctccaccgaa agaaaaagag tggcgaacgg 60 gtgagtaaca cgtgggtaac ctgcccatca gaaggggata acacttggaa acaggtgcta 120 ataccgtata acaagtaaaa ccgcatggtt ttactttgaa aggcgctttt gcgtcactga 180 tggatggacc cgcggtgcat tagctagttg gtgaggtaac ggctcaccaa ggcaacgatg 240 catagccgac ctgagagggt gatcggccac attgggactg agacacggcc caaactccta 300 cgggaggcag cagtagggaa tcttcggcaa tggacgaaag tctgaccgag caacgccgcg 360 tgagtgaaga aggttttcgg atcgtaaaac tctgttgtta gagaagaaca aggatgagag 420 taaaaagttc atcccttgac ggtatctaac cagaaagcca cggctaacta cgtgccagca 480 gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca 540 ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac 600 tgggaaactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg aaatgcgtag 660 atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggct 720 cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgag 780 tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta agcactccgc 840 ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc gcacaagcgg 900 tggagcatgt ggtttaattc gaagcaacgc gaagaacctt accaggtctt gacatccttt 960 gaccactcta gagatagagc ttccccttcg ggggcaaagt gacaggtggt gcatggttgt 1020 cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac ccttattgtt 1080 agttgccatc atttagttgg gcactctagc gagactgccg gtgacaaacc ggaggaaggt 1140 ggggatgacg tcaaatcatc atgcccctta tgacctgggc tacacacgtg ctacaatggg 1200 aagtacaacg agtcgcgaag tcgcgaggct aagctaatct cttaaagctt ctctcagttc 1260 ggattgtagg ctgcaactcg cctacatgaa gccggaatcg ctagtaatcg cggatcagca 1320 cgccgcggtg aatacgttcc cggg 1344

<210> 109

<211> 1309

<212> DNA

<213> Prior Sequence 10

<400> 109 tgcaagtcgt acgctggctt tttccaccgg agcttgctcc accggaaaaa gaagagtggc 60 gaacgggtga gtaacacgtg ggtaacctgc ccatcagaag gggataacac ttggaaacag 120 gtgctaatac cgtataacaa tcgaaaccgc atggttttga tttgaaaggc gctttcgggt 180 gtcgctgatg gatggacccg cggtgcatta gctagttggt gaggtaacgg ctcaccaagg 240 ccacgatgca tagccgacct gagagggtga tcggccacat tgggactgag acacggccca 300 aactcctacg ggaggcagca gtagggaatc ttcggcaatg gacgaaagtc tgaccgagca 360 acgccgcgtg agtgaagaag gttttcggat cgtaaaactc tgttgttaga gaagaacaag 420 gatgagagta actgttcatc ccttgacggt atctaaccag aaagccacgg ctaactacgt 480 gccagcagcc gcggtaatac gtaggtggca agcgttgtcc ggatttattg ggcgtaaagc 540 gagcgcaggc ggtttcttaa gtctgatgtg aaagcccccg gctcaaccgg ggagggtcat 600 tggaaactgg gagacttgag tgcagaagag gagagtggaa ttccatgtgt agcggtgaaa 660 tgcgtagata tatggaggaa caccagtggc gaaggcggct ctctggtctg taactgacgc 720 tgaggctcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa 780 cgatgagtgc taagtgttgg agggtttccg cccttcagtg ctgcagctaa cgcattaagc 840 actccgcctg gggagtacga ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca 900 caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac 960 atcctttgac cactctagag atagagcttc cccttcgggg gcaaagtgac aggtggtgca 1020 tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct 1080 tattgttagt tgccatcatt cagttgggca ctctagcaag actgccggtg acaaaccgga 1140 ggaaggtggg gatgacgtca aatcatcatg ccccttatga cctgggctac acacgtgcta 1200 caatgggaag tacaacgagt tgcgaagtcg cgaggctaag ctaatctctt aaagcttctc 1260 tcagttcgga ttgcaggctg caactcgcct gcatgaagcc ggaatcgct 1309

<210> 110

<211> 1366

<212> DNA

<213> Prior Sequence 11

<400> 110 ctttttccac cggagcttgc tccaccggaa aaagaagagt ggcgaacggg tgagtaacac 60 gtgggtaacc tgcccatcag aaggggataa cacttggaaa caggtgctaa taccgtataa 120 caatcgaaac cgcatggttt tgatttgaaa ggcgctttcg ggtgtcgctg atggatggac 180 ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca aggccacgat gcatagccga 240 cctgagaggg tgatcggcca cattgggact gagacacggc ccaaactcct acgggaggca 300 gcagtaggga atcttcggca atggacgaaa gtctgaccga gcaacgccgc gtgagtgaag 360 aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac aaggatgaga gtaactgttc 420 atcccttgac ggtatctaac cagaaagcca cggctaacta cgtgccagca gccgcggtaa 480 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca ggcggtttct 540 taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac tgggagactt 600 gagtgcagaa gaggagagtg gaattccatg tgtagcggtg aaatgcgtag atatatggag 660 gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggct cgaaagcgtg 720 gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgag tgctaagtgt 780 tggagggttt ccgcccttca gtgctgcagc taacgcatta agcactccgc ctggggagta 840 cgaccgcaag gttgaaactc aaaggaattg acgggggccc gcacaagcgg tggagcatgt 900 ggtttaattc gaagcaacgc gaagaacctt accaggtctt gacatccttt gaccactcta 960 gagatagagc ttccccttcg ggggcaaagt gacaggtggt gcatggttgt cgtcagctcg 1020 tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac ccttattgtt agttgccatc 1080 atttagttgg gcactctagc aagactgccg gtgacaaacc ggaggaaggt ggggatgacg 1140 tcaaatcatc atgcccctta tgacctgggc tacacacgtg ctacaatggg aagtacaacg 1200 agttgcgaag tcgcgaggct aagctaatct cttaaagctt ctctcagttc ggattgcagg 1260 ctgcaactcg cctgcatgaa gccggaatcg ctagtaatcg cggatcagca cgccgcggtg 1320 aatacgttcc cgggccttgt acacaccgcc cgtcacacca cgagag 1366

<210> 111

<211> 1116

<212> DNA

<213> Prior Sequence 12

<400> 111 gcggcgtgcc taatacatgc aagtcgtacg cttctttttc caccggagct tgctccaccg 60 gaaaaagaag agtggcgaac gggtgagtaa cacgtgggta acctgcccat cagaagggga 120 taacacttgg aaacaggtgc taataccgta taacaatcga aaccgcatgg ttttgatttg 180 aaaggcgctt tcgggtgtcg ctgatggatg gacccgcggt gcattagcta gttggtgagg 240 taacggctca ccaaggccac gatgcatagc cgacctgaga gggtgatcgg ccacattggg 300 actgagacac ggcccaaact cctacgggag cgagcagtag ggaatcttcg gcaatggacg 360 aaagtctgac cgagcaacgc cgcgtgagtg aagaaggttt tcggatcgta aaactctgtt 420 gttagagaag aacaaggatg agagtaactg ttcatccctt gacggtatct aaccagaaag 480 ccacggctaa ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg ttgtccggat 540 ttattgggcg taaagcgagc gcaggcggtt tcttaagtct gatgtgaaag cccccggctc 600 aaccggggag ggtcattgga aactgggaga cttgagtgca gaagaggaga gtggaattcc 660 atgtgtagcg gtgaaatgcg tagatatatg gaggaacacc agtggcgaag gcggctctct 720 ggtctgtaac tgacgctgag gctcgaaagc gtggggagca aacaggatta gataccctgg 780 tagtccacgc cgtaaacgat gagtgctaag tgttggaggg tttccgccct tcagtgctgc 840 agctaacgca ttaagcactc cgcctgggga gtacgaccgc aaggttgaaa ctcaaaggaa 900 ttgacggggg cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaac 960 cttaccaggt cttgacatcc tttgaccact ctagagatag agcttcccct tcgggggcaa 1020 agtgacaggt ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg 1080 caacgagcgc aacccttatt gttagttgcc atcatt 1116

<210> 112

<211> 1323

<212> DNA

<213> Prior Sequence 13

<400> 112 agtggcgaac gggtgagtaa cacgtgggta acctgcccat cagaagggga taacacttgg 60 aaacaggtgc taataccgta taacaatcga aaccgcatgg tttcgatttg aaaggcgctt 120 tcgggtgtcg ctgatggatg gacccgcggt gcattagcta gttggtgagg taacggctca 180 ccaaggcgac gatgcatagc cgacctgaga gggtgatcgg ccacattggg actgagacac 240 ggcccaaact cctacgggag gcagcagtag ggaatcttcg gcaatggacg aaagtctgac 300 cgagcaacgc cgcgtgagtg aagaaggttt tcggatcgta aaactctgtt gttagagaag 360 aacaaggatg agagtaactg ttcatccctt gacggtatct aaccagaaag ccacggctaa 420 ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg ttgtccggat ttattgggcg 480 taaagcgagc gcaggcggtt tcttaagtct gatgtgaaag cccccggctc aaccggggag 540 ggtcattgga aactgggaga cttgagtgca gaagaggaga gtggaattcc atgtgtagcg 600 gtgaaatgcg tagatatatg gaggaacacc agtggcgaag gcggctctct ggtctgtaac 660 tgacgctgag gctcgaaagc gtggggagca aacaggatta gataccctgg tagtccacgc 720 cgtaaacgat gagtgctaag tgttggaggg tttccgccct tcagtgctgc agctaacgca 780 ttaagcactc cgcctgggga gtacgaccgc aaggttgaaa ctcaaaggaa ttgacggggg 840 cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt 900 cttgacatcc tttgaccact ctagagatag agcttcccct tcgggggcaa agtgacaggt 960 ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc 1020 aacccttatt gttagttgcc atcattcagt tgggcactct agcaagactg ccggtgacaa 1080 accggaggaa ggtggggatg acgtcaaatc atcatgcccc ttatgacctg ggctacacac 1140 gtgctacaat gggaagtaca acgagtcgca aagtcgcgag gctaagctaa tctcttaaag 1200 cttctctcag ttcggattgt aggctgcaac tcgcctacat gaagccggaa tcgctagtaa 1260 tcgcggatca gcacgccgcg gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca 1320 cca 1323

<210> 113

<211> 1417

<212> DNA <213> Prior Sequence 14 <400> 113 agagtttgat cctggctcag gacgaacgct ggcggcgtgc ctaatacatg caagtcgtac 60 gctttttctt tcatcggagc ttgctccacc gaaagaaaag gagtggcgaa cgggtgagta 120 acacgtgggt aacctgccca tcagaagggg ataacacttg gaaacaggtg ctaataccgt 180 ataacaatcg aaaccgcatg gttttgattt gaaaggcgct ttcgggtgtc gctgatggat 240 ggacccgcgg tgcattagct agttggtgag gtaacggctc accaaggcca cgatgcatag 300 ccgacctgag agggtgatcg gccacattgg gactgagaca cggcccaaac tcctacggga 360 ggcagcagta gggaatcttc ggcaatggac gaaagtctga ccgagcaacg ccgcgtgagt 420 gaagaaggtt ttcggatcgt aaaactctgt tgttagagaa gaacaaggat gagagtaact 480 gttcatccct tgacggtatc taaccagaaa gccacggcta actacgtgcc agcagccgcg 540 gtaatacgta ggtggcaagc gttgtccgga tttattgggc gtaaagcgag cgcaggcggt 600 ttcttaagtc tgatgtgaaa gcccccggct caaccgggga gggtcattgg aaactgggag 660 acttgagtgc agaagaggag agtggaattc catgtgtagc ggtgaaatgc gtagatatat 720 ggaggaacac cagtggcgaa ggcgactctc tggtctgtaa ctgacgctga ggctcgaaag 780 cgtggggagc aaacaggatt agataccctg gtagtccacg ccgtaaacga tgagtgctaa 840 gtgttggagg gtttccgccc ttcagtgctg cagctaacgc attaagcact ccgcctgggg 900 agtacgaccg caaggttgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc 960 atgtggttta attcgaagca acgcgaagaa ccttaccagg tcttgacatc ctttgaccac 1020 tctagagata gagcttcccc ttcgggggca aagtgacagg tggtgcatgg ttgtcgtcag 1080 ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccttat tgttagttgc 1140 catcatttag ttgggcactc tagcaagact gccggtgaca aaccggagga aggtggggat 1200 gacgtcaaat catcatgccc cttatgacct gggctacaca cgtgctacaa tgggaagtac 1260 aacgagtcgc gaagtcgcga ggctaagcta atctcttaaa gcttctctca gttcggattg 1320 taggctgcaa ctcgcctaca tgaagccgga atcgctagta atcgcggatc agcacgccgc 1380 ggtgaatacg ttcccgggcc ttgtacacac cgcccgt 1417

<210> 114

<211> 1535

<212> RNA

<213> prior sequence 15

<400> 114 ccuggcucag gacgaacgcu ggcggcgugc cuaauacaug caagucgaac gcuucuuuuu 60 ccaccggagc uugcuccacc ggaaaaagag gaguggcgaa cgggugagua acacgugggu 120 aaccugccca ucagaagggg auaacacuug gaaacaggug cuaauaccgu auaacaaucg 180 aaaccgcaug guuuugauuu gaaaggcgcu uucggguguc gcugauggau ggacccgcgg 240 ugcauuagcu aguuggugag guaacggcuc accaaggcga cgaugcauag ccgaccugag 300 agggugaucg gccacauugg gacugagaca cggcccaaac uccuacggga ggcagcagua 360 gggaaucuuc ggcaauggac gaaagucuga ccgagcaacg ccgcgugagu gaagaagguu 420 uucggaucgu aaaacucugu uguuagagaa gaacaaggau gagaguaacu guucaucccu 480 ugacgguauc uaaccagaaa gccacggcua acuacgugcc agcagccgcg guaauacgua 540 gguggcaagc guuguccgga uuuauugggc guaaagcgag cgcaggcggu uucuuaaguc 600 ugaugugaaa gcccccggcu caaccgggga gggucauugg aaacugggag acuugagugc 660 agaagaggag aguggaauuc cauguguagc ggugaaaugc guagauauau ggaggaacac 720 caguggcgaa ggcggcucuc uggucuguaa cugacgcuga ggcucgaaag cguggggagc 780 aaacaggauu agauacccug guaguccacg ccguaaacga ugagugcuaa guguuggagg 840 guuuccgccc uucagugcug cagcuaacgc auuaagcacu ccgccugggg aguacgaccg 900 caagguugaa acucaaagga auugacgggg gcccgcacaa gcgguggagc augugguuua 960 auucgaagca acgcgaagaa ccuuaccagg ucuugacauc cuuugaccac ucuagagaua 1020 gagcuucccc uucgggggca aagugacagg uggugcaugg uugucgucag cucgugucgu 1080 gagauguugg guuaaguccc gcaacgagcg caacccuuau uguuaguugc caucauuuag 1140 uugggcacuc uagcaagacu gccggugaca aaccggagga agguggggau gacgucaaau 1200 caucaugccc cuuaugaccu gggcuacaca cgugcuacaa ugggaaguac aacgagucgc 1260 aaagucgcga ggcuaagcua aucucuuaaa gcuucucuca guucggauug uaggcugcaa 1320 cucgccuaca ugaagccgga aucgcuagua aucgcggauc agcacgccgc ggugaauacg 1380 uucccgggcc uuguacacac cgcccgucac accacgagag uuuguaacac ccgaagucgg 1440 ugagguaacc uuuuggagcc agccgccuaa ggugggauag augauugggg ugaagucgua 1500 acaagguagc cguaucggaa ggugcggcug gauca 1535

<210> 115

<211> 1401

<212> DNA

<213> Enterococcus sp. Pep7

<220>

<221 > misc_feature

<222> (333).. (333)

<223> n is a, c, g, or t

<400> 115 acgccttttt ctttcaccgg agcttgctcc accgaaagaa aaggagtggc gaacgggtga 60 gtaacacgtg ggtaacctgc ccatcagaag gggataacac ttggaaacag gtgctaatac 120 cgtataacaa tcgaaaccgc atggtttcgg tttgaaaggc gcttttgcgt cactgatgga 180 tggacccgcg gtgcattagc tagttggtga ggtaacggct caccaaggca acgatgcata 240 gccgacctga gagggtgatc ggccacattg ggactgagac acggcccaaa ctcctacggg 300 aggcagcagt agggaatctt cggcaatgga cgnaagtctg accgagcaac gccgcgtgag 360 tgaagaaggt tttcggatcg taaaactctg ttgttagaga agaacaagga tgagagtaga 420 atgttcatcc cttgacggta tctaaccaga aagccacggc taactacgtg ccagcagccg 480 cggtaatacg taggtggcaa gcgttgtccg gatttattgg gcgtaaagcg agcgcaggcg 540 gtttcttaag tctgatgtga aagcccccgg ctcaaccggg gagggtcatt ggaaactggg 600 aaacttgagt gcagaagagg agagtggaat tccatgtgta gcggtgaaat gcgtagatat 660 atggaggaac accagtggcg aaggcggctc tctggtctgt aactgacgct gaggctcgaa 720 agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccgtaaac gatgagtgct 780 aagtgttgga gggtttccgc ccttcagtgc tgcagctaac gcattaagca ctccgcctgg 840 ggagtacgac cgcaaggttg aaactcaaag gaattgacgg gggcccgcac aagcggtgga 900 gcatgtggtt taattcgaag caacgcgaag aaccttacca ggtcttgaca tcctttgacc 960 actctagaga tagagcttcc ccttcggggg caaagtgaca ggtggtgcat ggttgtcgtc 1020 agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag cgcaaccctt attgttagtt 1080 gccatcattt agttgggcac tctagcgaga ctgccggtga caaaccggag gaaggtgggg 1140 atgacgtcaa atcatcatgc cccttatgac ctgggctaca cacgtgctac aatgggaagt 1200 acaacgagtc gcgaagtcgc gaggctaagc taatctctta aagcttctct cagttcggat 1260 tgtaggctgc aactcgccta catgaagccg gaatcgctag taatcgcgga tcagcacgcc 1320 gcggtgaata cgttcccggg ccttgtacac accgcccgtc acaccacgag agtttgtaac 1380 acccgaagtc ggtgaggtaa c 1401

<210> 116

<211> 1295

<212> DNA

<213> Enterococcus gilvus 2

<400> 116 acgctttttc tttcaccgga gcttgctcca ccgaaagaaa aggagtggcg aacgggtgag 60 taacacgtgg gtaacctgcc catcagaagg ggataacact tggaaacagg tgctaatacc 120 gtataacaat agaaaccgca tggtttctat ttgaaaggcg cttttgcgtc actgatggat 180 ggacccgcgg tgcattagct agttggtgag gtaacggctc accaaggcaa cgatgcatag 240 ccgacctgag agggtgatcg gccacattgg gactgagaca cggcccaaac tcctacggga 300 ggcagcagta gggaatcttc ggcaatggac gaaagtctga ccgagcaacg ccgcgtgagt 360 gaagaaggtt ttcggatcgt aaaactctgt tgttagagaa gaacaaggat gagagtaaaa 420 tgttcatccc ttgacggtat ctgaccagaa agccacggct aactacgtgc cagcagccgc 480 ggtaatacgt aggtggcaag cgttgtccgg atttattggg cgtaaagcga gcgcaggcgg 540 tttcttaagt ctgatgtgaa agcccccggc tcaaccgggg agggtcattg gaaactggga 600 aacttgagtg cagaagagga gagtggaatt ccatgtgtag cggtgaaatg cgtagatata 660 tggaggaaca ccagtggcga aggcggctct ctggtctgta actgacgctg aggctcgaaa 720 gcgtggggag caaacaggat tagataccct ggtagtccac gccgtaaacg atgagtgcta 780 agtgttggag ggtttccgcc cttcagtgct gcagctaacg cattaagcac tccgcctggg 840 gagtacgacc gcaaggttga aactcaaagg aattgacggg ggcccgcaca agcggtggag 900 catgtggttt aattcgaagc aacgcgaaga accttaccag gtcttgacat cctttgacca 960 ctctagagat agagcttccc cttcgggggc aaagtgacag gtggtgcatg gttgtcgtca 1020 gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccctta ttgttagttg 1080 ccatcattta gttgggcact ctagcgagac tgccggtgac aaaccggagg aaggtgggga 1140 tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac acgtgctaca atgggaagta 1200 caacgagtcg cgaagtcgcg aggctaagct aatctcttaa agcttctctc agttcggatt 1260 gtaggctgca actcgcctac atgaagccgg aatcg 1295

<210> 117

<211> 1275

<212> DNA

<213> Enterococcus raffinosus 3

<400> 117 gggtgagtaa cacgtgggta acctgcccat cagaagggga taacacttgg aaacaggtgc 60 taataccgta taacaataga aaccgcatgg tttctatttg aaaggcgctt ttgcgtcact 120 gatggatgga cccgcggtgc attagctagt tggtgaggta acggctcacc aaggcaacga 180 tgcatagccg acctgagagg gtgatcggcc acattgggac tgagacacgg cccaaactcc 240 tacgggaggc agcagtaggg aatcttcggc aatggacgaa agtctgaccg agcaacgccg 300 cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt tagagaagaa caaggatgag 360 agtagaatgt tcatcccttg acggtatcta accagaaagc cacggctaac tacgtgccag 420 cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt aaagcgagcg 480 caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg gtcattggaa 540 actgggaaac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg tgaaatgcgt 600 agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact gacgctgagg 660 ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc gtaaacgatg 720 agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat taagcactcc 780 gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc 840 ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc ttgacatcct 900 ttgaccactc tagagataga gcttcccctt cgggggcaaa gtgacaggtg gtgcatggtt 960 gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca acccttattg 1020 ttagttgcca tcatttagtt gggcactcta gcgagactgc cggtgacaaa ccggaggaag 1080 gtggggatga cgtcaaatca tcatgcccct tatgacctgg gctacacacg tgctacaatg 1140 ggaagtacaa cgagtcgcga agtcgcgagg ctaagctaat ctcttaaagc ttctctcagt 1200 tcggattgta ggctgcaact cgcctacatg aagccggaat cgctagtaat cgcggatcag 1260 cacgccgcgg tgaca 1275

<210> 118

<211> 1183

<212> DNA

<213> Enterococcus sp. Da-20

<400> 118 gtcgaacgct ttttctttca ccggagcttg ctccaccgaa agaaaaggag tggcgaacgg 60 gtgagtaaca cgtgggtaac ctgcccatca gaaggggata acacttggaa acaggtgcta 120 ataccgtata acaatcgaaa ccgcatggtt tcaatttgaa aggcgctttt gcgtcactga 180 tggatggacc cgcggtgcat tagctagttg gtgaggtaac ggctcaccaa ggcaacgatg 240 catagccgac ctgagagggt gatcggccac attgggactg agacacggcc caaactccta 300 cgggaggcag cagtagggaa tcttcggcaa tggacgcaag tctgaccgag caacgccgcg 360 tgagtgaaga aggttttcgg atcgtaaaac tctgttgtta gagaagaaca aggatgagag 420 tagaatgttc atcccttgac ggtatctaac cagaaagcca cggctaacta cgtgccagca 480 gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca 540 ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac 600 tgggaaactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg aaatgcgtag 660 atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggct 720 cgaaagcgtg gggagcaaac aggattagat accctggcag tccacgccgt aaacgatgag 780 tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta agcactccgc 840 ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc gcacaagcgg 900 tggagcatgt ggtttaattc gaagcaacgc gaagaacctt accaggtctt gacatccttt 960 gaccactcta gagatagagc ttccccttcg ggggcaaagt gacaggtggt gcatggttgt 1020 cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac ccttattgtt 1080 agttgccatc atttagttgg gcactctagc gagactgccg gtgacaaacc ggaggaaggt 1140 ggggatgacg tcaaatcatc atgcccctta tgacctgggc tac 1183

<210> 119

<211> 1133

<212> DNA

<213> Enterococcus sp. 936B6_12ASB

<400> 119 gtcgaacgct ttttctttca ccggagcttg ctccaccgaa agaaaaggag tggcgaacgg 60 gtgagtaaca cgtgggtaac ctgcccatca gaaggggata acacttggaa acaggtgcta 120 ataccgtata acaatcgaaa ccgcatggtt tcggtttgaa aggcgctttt gcgtcactga 180 tggatggacc cgcggtgcat tagctagttg gtgaggtaac ggctcaccaa ggcaacgatg 240 catagccgac ctgagagggt gatcggccac attgggactg agacacggcc caaactccta 300 cgggaggcag cagtagggaa tcttcggcaa tggacgsaag tctgaccgag caacgccgcg 360 tgagtgaaga aggttttcgg atcgtaaaac tctgttgtta gagaagaaca aggatgagag 420 tagaatgttc atcccttgac ggtatctaac cagaaagcca cggctaacta cgtgccagca 480 gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca 540 ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac 600 tgggaaactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg aaatgcgtag 660 atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggct 720 cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgag 780 tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta agcactccgc 840 ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc gcacaagcgg 900 tggagcatgt ggtttaattc gaagcaacgc gaagaacctt accaggtctt gacatccttt 960 gaccactcta gagatagagc ttccccttcg ggggcaaagt gacaggtggt gcatggttgt 1020 cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac ccttattgtt 1080 agttgccatc atttagttgg gcactctagc gagactgccg gtgacaaacc gga 1133

<210> 120

<211> 1422

<212> DNA

<213> Prior Sequence 16

<400> 120 acgctttttc tttcaccgga gcttgctcca ccgaaagaaa aggagtggcg aacgggtgag 60 taacacgtgg gtaacctgcc catcagaagg ggataacact tggaaacagg tgctaatacc 120 gtataacaat cgaaaccgca tggtttcgat ttgaaaggcg cttttgcgtc actgatggat 180 ggacccgcgg tgcattagct agttggtgag gtaacggctc accaaggcaa cgatgcatag 240 ccgacctgag agggtgatcg gccacattgg gactgagaca cggcccaaac tcctacggga 300 ggcagcagta gggaatcttc ggcaatggac gcaagtctga ccgagcaacg ccgcgtgagt 360 gaagaaggtt ttcggatcgt aaaactctgt tgttagagaa gaacaaggat gagagtagaa 420 tgttcatccc ttgacggtat ctaaccagaa agccacggct aactacgtgc cagcagccgc 480 ggtaatacgt aggtggcaag cgttgtccgg atttattggg cgtaaagcga gcgcaggcgg 540 tttcttaagt ctgatgtgaa agcccccggc tcaaccgggg agggtcattg gaaactggga 600 aacttgagtg cagaagagga gagtggaatt ccatgtgtag cggtgaaatg cgtagatata 660 tggaggaaca ccagtggcga aggcggctct ctggtctgta actgacgctg aggctcgaaa 720 gcgtggggag caaacaggat tagataccct ggtagtccac gccgtaaacg atgagtgcta 780 agtgttggag ggtttccgcc cttcagtgct gcagctaacg cattaagcac tccgcctggg 840 gagtacgacc gcaaggttga aactcaaagg aattgacggg ggcccgcaca agcggtggag 900 catgtggttt aattcgaagc aacgcgaaga accttaccag gtcttgacat cctttgacca 960 ctctagagat agagcttccc cttcgggggc aaagtgacag gtggtgcatg gttgtcgtca 1020 gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccctta ttgttagttg 1080 ccatcattta gttgggcact ctagcgagac tgccggtgac aaaccggagg aaggtgggga 1140 tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac acgtgctaca atgggaagta 1200 caacgagtcg cgaagtcgcg aggctaagct aatctcttaa agcttctctc agttcggatt 1260 gtaggctgca actcgcctac atgaagccgg aatcgctagt aatcgcggat cagcacgccg 1320 cggtgaatac gttcccgggc cttgtacaca ccgcccgtca caccacgaga gtttgtaaca 1380 cccgaagtcg gtgaggtaac cttttggagc cagccgccta ag 1422

<210> 121

<211> 1431

<212> DNA

<213> Prior Sequence 17

<400> 121 tgcagtcgaa cgctttttct ttcaccggag cttgctccac cgaaagaaaa ggagtggcga 60 acgggtgagt aacacgtggg taacctgccc atcagaaggg gataacactt ggaaacaggt 120 gctaataccg tataacaatc gaaaccgcat ggtttcggtt tgaaaggcgc ttttgcgtca 180 ctgatggatg gacccgcggt gcattagcta gttggtgagg taacggctca ccaaggcaac 240 gatgcatagc cgacctgaga gggtgatcgg ccacattggg actgagacac ggcccaaact 300 cctacgggag gcagcagtag ggaatcttcg gcaatggacg caagtctgac cgagcaacgc 360 cgcgtgagtg aagaaggttt tcggatcgta aaactctgtt gttagagaag aacaaggatg 420 agagtaaaat gttcatccct tgacggtatc taaccagaaa gccacggcta actacgtgcc 480 agcagccgcg gtaatacgta ggtggcaagc gttgtccgga tttattgggc gtaaagcgag 540 cgcaggcggt ttcttaagtc tgatgtgaaa gcccccggct caaccgggga gggtcattgg 600 aaactgggaa acttgagtgc agaagaggag agtggaattc catgtgtagc ggtgaaatgc 660 gtagatatat ggaggaacac cagtggcgaa ggcggctctc tggtctgtaa ctgacgctga 720 ggctcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg ccgtaaacga 780 tgagtgctaa gtgttggagg gtttccgccc ttcagtgctg cagctaacgc attaagcact 840 ccgcctgggg agtacgaccg caaggttgaa actcaaagga attgacgggg gcccgcacaa 900 gcggtggagc atgtggttta attcgaagca acgcgaagaa ccttaccagg tcttgacatc 960 ctttgaccac tctagagata gagcttcccc ttcgggggca aagtgacagg tggtgcatgg 1020 ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccttat 1080 tgttagttgc catcatttag ttgggcactc tagcgagact gccggtgaca aaccggagga 1140 aggtggggat gacgtcaaat catcatgccc cttatgacct gggctacaca cgtgctacaa 1200 tgggaagtac aacgagtcgc gaagtcgcga ggctaagcta atctcttaaa gcttctctca 1260 gttcggattg taggctgcaa ctcgcctaca tgaagccgga atcgctagta atcgcggatc 1320 agcacgccgc ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accacgagag 1380 tttgtaacac ccgaagtcgg tgaggtaacc ttttggagcc agccgcctaa g 1431

<210> 122

<211> 1410

<212> DNA

<213> Prior Sequence 18

<400> 122 agtcgaacgc tttttctttc accggagctt gctccaccga aagaaaagga gtggcgaacg 60 ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga aacaggtgct 120 aataccgtat aacaatcgaa accgcatggt ttcggtttga aaggcgcttt tgcgtcactg 180 atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca aggcaacgat 240 gcatagccga cctgagaggg tgatcggcca cattgggact gagacacggc ccaaactcct 300 acgggaggca gcagtaggga atcttcggca atggacgaaa gtctgaccga gcaacgccgc 360 gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac aaggatgaga 420 gtaaaatgtt catcccttga cggtatctaa ccagaaagcc acggctaact acgtgccagc 480 agccgcggta atacgtaggt ggcaagcgtt gtccggattt attgggcgta aagcgagcgc 540 aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg tcattggaaa 600 ctgggaaact tgagtgcaga agaggagagt ggaattccat gtgtagcggt gaaatgcgta 660 gatatatgga ggaacaccag tggcgaaggc ggctctctgg tctgtaactg acgctgaggc 720 tcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccg taaacgatga 780 gtgctaagtg ttggagggtt tccgcccttc agtgctgcag ctaacgcatt aagcactccg 840 cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc cgcacaagcg 900 gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaggtct tgacatcctt 960 tgaccactct agagatagag cttccccttc gggggcaaag tgacaggtgg tgcatggttg 1020 tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa cccttattgt 1080 tagttgccat catttagttg ggcactctag cgagactgcc ggtgacaaac cggaggaagg 1140 tggggatgac gtcaaatcat catgcccctt atgacctggg ctacacacgt gctacaatgg 1200 gaagtacaac gagtcgcgaa gtcgcgaggc taagctaatc tcttaaagct tctctcagtt 1260 cggattgtag gctgcaactc gcctacatga agccggaatc gctagtaatc gcggatcagc 1320 acgccgcggt gaatacgttc ccgggccttg tacacaccgc ccgtcacacc acgagagttt 1380 gtaacacccg aagtcggtga ggtaaccttt 1410

<210> 123

<211> 1295

<212> DNA

<213> Prior Sequence 19

<400> 123 acgctttttc tttcaccgga gcttgctcca ccgaaagaaa aggagtggcg aacgggtgag 60 taacacgtgg gtaacctgcc catcagaagg ggataacact tggaaacagg tgctaatacc 120 gtataacaat agaaaccgca tggtttctat ttgaaaggcg cttttgcgtc actgatggat 180 ggacccgcgg tgcattagct agttggtgag gtaacggctc accaaggcaa cgatgcatag 240 ccgacctgag agggtgatcg gccacattgg gactgagaca cggcccaaac tcctacggga 300 ggcagcagta gggaatcttc ggcaatggac gaaagtctga ccgagcaacg ccgcgtgagt 360 gaagaaggtt ttcggatcgt aaaactctgt tgttagagaa gaacaaggat gagagtaaaa 420 tgttcatccc ttgacggtat ctgaccagaa agccacggct aactacgtgc cagcagccgc 480 ggtaatacgt aggtggcaag cgttgtccgg atttattggg cgtaaagcga gcgcaggcgg 540 tttcttaagt ctgatgtgaa agcccccggc tcaaccgggg agggtcattg gaaactggga 600 aacttgagtg cagaagagga gagtggaatt ccatgtgtag cggtgaaatg cgtagatata 660 tggaggaaca ccagtggcga aggcggctct ctggtctgta actgacgctg aggctcgaaa 720 gcgtggggag caaacaggat tagataccct ggtagtccac gccgtaaacg atgagtgcta 780 agtgttggag ggtttccgcc cttcagtgct gcagctaacg cattaagcac tccgcctggg 840 gagtacgacc gcaaggttga aactcaaagg aattgacggg ggcccgcaca agcggtggag 900 catgtggttt aattcgaagc aacgcgaaga accttaccag gtcttgacat cctttgacca 960 ctctagagat agagcttccc cttcgggggc aaagtgacag gtggtgcatg gttgtcgtca 1020 gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccctta ttgttagttg 1080 ccatcattta gttgggcact ctagcgagac tgccggtgac aaaccggagg aaggtgggga 1140 tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac acgtgctaca atgggaagta 1200 caacgagtcg cgaagtcgcg aggctaagct aatctcttaa agcttctctc agttcggatt 1260 gtaggctgca actcgcctac atgaagccgg aatcg 1295

<210> 124

<211> 1524

<212> DNA <213> Prior Sequence 20

<400> 124 ccgtagagtt tgatcctggc tcaggacgaa cgctggcggc gtgcctaata catgcaagtc 60 gaacgctttt tctttcaccg gagcttgctc caccgaaaga aaaggagtgg cgaacgggtg 120 agtaacacgt gggtaacctg cccatcagaa ggggataaca cttggaaaca ggtgctaata 180 ccgtataaca atcgaaaccg catggtttcg gtttgaaagg cgcttttgcg tcactgatgg 240 atggacccgc ggtgcattag ctagttggtg aggtaacggc tcaccaaggc aacgatgcat 300 agccgacctg agagggtgat cggccacatt gggactgaga cacggcccaa actcctacgg 360 gaggcagcag tagggaatct tcggcaatgg acgcaagtct gaccgagcaa cgccgcgtga 420 gtgaagaagg ttttcggatc gtaaaactct gttgttagag aagaacaagg atgagagtag 480 aatgttcatc ccttgacggt atctaaccag aaagccacgg ctaactacgt gccagcagcc 540 gcggtaatac gtaggtggca agcgttgtcc ggatttattg ggcgtaaagc gagcgcaggc 600 ggtttcttaa gtctgatgtg aaagcccccg gctcaaccgg ggagggtcat tggaaactgg 660 gaaacttgag tgcagaagag gagagtggaa ttccatgtgt agcggtgaaa tgcgtagata 720 tatggaggaa caccagtggc gaaggcggct ctctggtctg taactgacgc tgaggctcga 780 aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa cgatgagtgc 840 taagtgttgg agggtttccg cccttcagtg ctgcagctaa cgcattaagc actccgcctg 900 gggagtacga ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca caagcggtgg 960 agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac atcctttgac 1020 cactctagag atagagcttc cctgggggca aagtgacagg tggtgcatgg ttgtcgtcag 1080 ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccttat tgttagttgc 1140 catcatttag ttgggcactc tagcgagact gccggtgaca aaccggagga aggtggggat 1200 gacgtcaaat catcatgccc cttatgacct gggctacaca cgtgctacaa tgggaagtac 1260 aacgagtcgc gaagtcgcga ggctaagcta atctcttaaa gcttctctca gttcggattg 1320 taggctgcaa ctcgcctaca tgaagccgga atcgctagta atcgcggatc agcacgccgc 1380 ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac 1440 ccgaagtcgg tgaggtaacc ttttggagcc agccgcctaa ggtgggatag atgattgggg 1500 tgaagtcgta acaaggtagc cgtc 1524

<210> 125

<211> 1521

<212> DNA

<213> Prior Sequence 21

<400> 125 ctcaggacga acgctggcgg cgtgcctaat acatgcaagt cgaacgcttt ttctttcacc 60 ggagcttgct ccaccgaaag aaaaggagtg gcgaacgggt gagtaacacg tgggtaacct 120 gcccatcaga aggggataac acttggaaac aggtgctaat accgtataac aatcgaaacc 180 gcatggtttc ggtttgaaag gcgcttttgc gtcactgatg gatggacccg cggtgcatta 240 gctagttggt gaggtaacgg ctcaccaagg caacgatgca tagccgacct gagagggtga 300 tcggccacat tgggactgag acacggccca aactcctacg ggaggcagca gtagggaatc 360 ttcggcaatg gacgcaagtc tgaccgagca acgccgcgtg agtgaagaag gttttcggat 420 cgtaaaactc tgttgttaga gaagaacaag gatgagagta gaaygttcat cccttgacgg 480 tatctaacca gaaagccacg gctaactacg tgccagcagc cgcggtaata cgtaggtggc 540 aagcgttgtc cggatttatt gggcgtaaag cgagcgcagg cggtttctta agtctgatgt 600 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggaaacttga gtgcagaaga 660 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg 720 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag 780 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc 840 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt 900 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga 960 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga gatagagctt 1020 ccccttcggg ggcaaagtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg 1080 ttgggttaag tcccgcaacg agcgcaaccc ttattgttag ttgccatcat ttagttgggc 1140 actctagcga gactgccggt gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat 1200 gccccttatg acctgggcta cacacgtgct acaatgggaa gtacaacgag tcgcgaagtc 1260 gcgaggctaa grtaatctyt taaagcttct ctcagttcgg attgtaggct graactcgcc 1320 tacatgaagc cggaatcgct agtaatcgcg gatcagcacg ccgcggtgaa tacgttcccg 1380 ggccttgtac acaccgcrcg tcacaccacg agagtttgta acacccgaag tcggtgaggt 1440 aaccttttgg agccagccgc ctaaggtggg atagatgatt ggggtgaagt cgtaacaagg 1500 tagccgtatc ggaaggtgcg g 1521

<210> 126

<211> 1893

<212> DNA

<213> Enterococcus raffinosus 4

<220>

<221 > misc_feature

<222> (11 ).. (12)

<223> n is a, c, g, or t <400> 126 agagtttgat nntggctcag gacgaacgct ggcggcgtgc ctaatacatg caagtcgaac 60 gccttttctt tcaccggagc ttgctccacc gaaagaaaag gagtggcgaa cgcgtgagta 120 acacgtgggt aacctgccca tcagaagggg ataacacttg gaaacaggtg ctaataccgt 180 ataacaatag aaaccgcatg gtttctattt gaaaggcgct tttgcgtcac tgatggatgg 240 acccgcggtg cattagctag ttggtgaggt aacggctcac caaggcaacg atgcatagcc 300 gacctgagag ggtgatcggc cacattggga ctgagacacg gcccaaactc ctacgggagg 360 cagcagtagg gaatcttcgg caatggacga aagtctgacc gagcaacgcc gcgtgagtga 420 agaaggtttt cggatcgtaa aactctgttg ttagagaaga acaaggatga gagtagaatg 480 ttcatccctt gacggtatct aaccagaaag ccacggctaa ctaacgtgcc agcagccgcg 540 gtaatacgta gagtggcaag cgttgtccgg atttattggg cgtaaagcga gcgcaggcgg 600 tttcttaagt ctgatgtgaa agcccccggc tcaaccgggg agggtcattg gaaactggga 660 aacttgagtg cagaagagga gagtggaatt ccatgtgtag cggtgaaatg cgtagatata 720 tggaggaaca ccagtggcga aggcggctct ctggtctgta actgacgctg aggctcgaaa 780 gcgtggggag caaacaggat tagataccct ggtagtccac gccgtaaacg atgagtgcta 840 agtgttggag ggtttcggcc cttcagtgct gcagctaacg cattaagcac tccgcctggg 900 gagtacgacc gcaaggttga aactcaaagg aattgacggg ggcccgcaca agcggtggag 960 catgtggttt aattcgaagc aacgcgaaga accttaccag gtcttgacat cctttgacca 1020 ctctagagat agagcttccc cttcgggggc aaagtgacag gtggtgcatg gttgtcgtca 1080 gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccctta ttgttagttg 1140 ccatcattta gttgggcact ctagcgagac tgccggtgac aaaccggagg aaggtgggga 1200 tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac acgtgctaca atgggaagta 1260 caacgagtcg cgaagtcgcg aggctaagct aatctcttaa agcttctctc agttcggatt 1320 gtaggctgca actcgcctac atgaagccgg aatcgctagt aatcgcggat cagcacgccg 1380 cggtgaatac gttcccgggc cttgtacaca ccgcccgtca caccacgaga gtttgtaaca 1440 cccgaagtcg gtgaggtaac cttttggagc cagccgccta aggtgggata gatgattggg 1500 gtgaagtcgt aacaaggtag ccgtatcgaa gggtgcggct ggatcacctc ctttctaagg 1560 aatattacgg aaactacaca ggttgcttta ttcgttcagt tttgagaggt ctactctcaa 1620 atcgttcatt gaacactgga tacagaaacg ataatttcta caaaccgaga acacgcgttg 1680 aatgagtttt ttaataagtc attgctattt tttctgattg tgagtctatc gctagtggac 1740 actcacccct accgtagtag ataagttagt gaataggcgc cgtgatgcgt gtcttgcgcg 1800 tgaggcggcc tacacgatat gctcggcact gtatgctgcg atcgtgctgc gcacacgata 1860 tgcttcgggg agctgtaagt aattctttcg ggg 1893

<210> 127

<211> 1701

<212> DNA

<213> Enterococcus malodoratus 3

<220>

<221 > misc_feature

<222> (11 ).. (12)

<223> n is a, c, g, or t

<400> 127 agagtttgat nntggctcga cgaacgctgg cggcgtgcct aatacatgca agtcgaacgc 60 tttttctttc accgagcttg ctccaccgaa agaaaaggag tcgcgaacgg ctgagtaaca 120 cgtgggtaac ctgcccatca gaaggagata acacttgaaa caggtgctaa taccgtataa 180 cagtagaaac cgcatggttt ctatttgaaa ggcgcttttg cgtcactgat ggatggaccc 240 gcggtgcatt agctagttgg tgaggtaacg gctcaccaag gcaacgatgc atagccgacc 300 tgagagggtg atcggccaca ttgggactga gacacggccc aaactcctac gggaggcagc 360 agtagggaat cttcggcaat ggacgaaagt ctgaccgagc aacgccgcgt gagtgaagaa 420 ggttttcgga tcgtaaaact ctgttgttag agaagaacaa ggatgagaga taaaacgttc 480 atcccttgac ggtatctaac cagaaagcca cggctaacta cgtgccagca gccgcggtaa 540 tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca ggcggtttct 600 taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac tgggaaactt 660 gagtgcagaa gaggagagtg gaattccatg tgtagcggtg aaatgcgtag atatatggag 720 gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggct cgaaagcgtg 780 gggagacaaa caggattaga taccctggta gtccacgccg taaacgatga gtgctaagtg 840 ttggagggtt tccgcccttc agtgctgcag ctaacgcatt aagcactccg cctggggagt 900 acgaccgcaa ggttgaaact caaaggaatt gacgggggcc cgcacaagcg tggagcatgt 960 ggtttaattc gaagcaacgc gaagaacctt accaggtctt gacatccttt gaccactcta 1020 gagatagagc ttccccttcg ggggcaaagt gacaggtggt gcatggttgt cgtcagctcg 1080 tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac ccttattgtt agttgccatc 1140 atttagttgg gcactctagc gagactgccg gtgacaaacc ggaggaaggt ggggatgacg 1200 tcaaatcatc atgcccctta tgacctgggc tacacacgtg ctacaatggg aagtacaacg 1260 agtcgcgaag tcgcgaggct aagctaatct cttaaagctt ctctcagttc ggattgtagg 1320 ctgcaactcg cctacatgaa gccggaatcg ctagtaatcg cggatcagca cgccgcggtg 1380 aatacgttcc cgggccttgt acacaccgcc cgtcacacca cgagagtttg taacacccga 1440 agtcggtgag gtaacctttt ggagccagcc gcctaaggtg tgatagatga ttggggtgaa 1500 gtcgtaacaa ggtagccgta tcggaaggtg cggctggatc acctcctttc taaggaatat 1560 tacggagact acacaggttg ctttattcgt tcagttttga gaggtttact ctcaaaaatc 1620 gttcattgaa actggtacga caaccaaaca aaccggaaca ccgcgttgat gagtttttta 1680 atagttcatt gctatttttt t 1701

<210> 128

<211> 1535

<212> DNA

<213> Enterococcus hirae

<400> 128 cctggctcag gacgaacgct ggcggcgtgc ctaatacatg caagtcgaac gcttcttttt 60 ccaccggagc ttgctccacc ggaaaaagag gagtggcgaa cgggtgagta acacgtgggt 120 aacctgccca tcagaagggg ataacacttg gaaacaggtg ctaataccgt ataacaatcg 180 aaaccgcatg gttttgattt gaaaggcgct ttcgggtgtc gctgatggat ggacccgcgg 240 tgcattagct agttggtgag gtaacggctc accaaggcga cgatgcatag ccgacctgag 300 agggtgatcg gccacattgg gactgagaca cggcccaaac tcctacggga ggcagcagta 360 gggaatcttc ggcaatggac gaaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt 420 ttcggatcgt aaaactctgt tgttagagaa gaacaaggat gagagtaact gttcatccct 480 tgacggtatc taaccagaaa gccacggcta actacgtgcc agcagccgcg gtaatacgta 540 ggtggcaagc gttgtccgga tttattgggc gtaaagcgag cgcaggcggt ttcttaagtc 600 tgatgtgaaa gcccccggct caaccgggga gggtcattgg aaactgggag acttgagtgc 660 agaagaggag agtggaattc catgtgtagc ggtgaaatgc gtagatatat ggaggaacac 720 cagtggcgaa ggcggctctc tggtctgtaa ctgacgctga ggctcgaaag cgtggggagc 780 aaacaggatt agataccctg gtagtccacg ccgtaaacga tgagtgctaa gtgttggagg 840 gtttccgccc ttcagtgctg cagctaacgc attaagcact ccgcctgggg agtacgaccg 900 caaggttgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta 960 attcgaagca acgcgaagaa ccttaccagg tcttgacatc ctttgaccac tctagagata 1020 gagcttcccc ttcgggggca aagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt 1080 gagatgttgg gttaagtccc gcaacgagcg caacccttat tgttagttgc catcatttag 1140 ttgggcactc tagcaagact gccggtgaca aaccggagga aggtggggat gacgtcaaat 1200 catcatgccc cttatgacct gggctacaca cgtgctacaa tgggaagtac aacgagtcgc 1260 aaagtcgcga ggctaagcta atctcttaaa gcttctctca gttcggattg taggctgcaa 1320 ctcgcctaca tgaagccgga atcgctagta atcgcggatc agcacgccgc ggtgaatacg 1380 ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac ccgaagtcgg 1440 tgaggtaacc ttttggagcc agccgcctaa ggtgggatag atgattgggg tgaagtcgta 1500 acaaggtagc cgtatcggaa ggtgcggctg gatca 1535

<210> 129

<211> 1535

<212> DNA

<213> Enterococcus hirae 2

<400> 129 cctggctcag gacgaacgct ggcggcgtgc ctaatacatg caagtcgaac gcttcttttt 60 ccaccggagc ttgctccacc ggaaaaagag gagtggcgaa cgggtgagta acacgtgggt 120 aacctgccca tcagaagggg ataacacttg gaaacaggtg ctaataccgt ataacaatcg 180 aaaccgcatg gttttgattt gaaaggcgct ttcgggtgtc gctgatggat ggacccgcgg 240 tgcattagct agttggtgag gtaacggctc accaaggcga cgatgcatag ccgacctgag 300 agggtgatcg gccacattgg gactgagaca cggcccaaac tcctacggga ggcagcagta 360 gggaatcttc ggcaatggac gaaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt 420 ttcggatcgt aaaactctgt tgttagagaa gaacaaggat gagagtaact gttcatccct 480 tgacggtatc taaccagaaa gccacggcta actacgtgcc agcagccgcg gtaatacgta 540 ggtggcaagc gttgtccgga tttattgggc gtaaagcgag cgcaggcggt ttcttaagtc 600 tgatgtgaaa gcccccggct caaccgggga gggtcattgg aaactgggag acttgagtgc 660 agaagaggag agtggaattc catgtgtagc ggtgaaatgc gtagatatat ggaggaacac 720 cagtggcgaa ggcggctctc tggtctgtaa ctgacgctga ggctcgaaag cgtggggagc 780 aaacaggatt agataccctg gtagtccacg ccgtaaacga tgagtgctaa gtgttggagg 840 gtttccgccc ttcagtgctg cagctaacgc attaagcact ccgcctgggg agtacgaccg 900 caaggttgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta 960 attcgaagca acgcgaagaa ccttaccagg tcttgacatc ctttgaccac tctagagata 1020 gagcttcccc ttcgggggca aagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt 1080 gagatgttgg gttaagtccc gcaacgagcg caacccttat tgttagttgc catcatttag 1140 ttgggcactc tagcaagact gccggtgaca aaccggagga aggtggggat gacgtcaaat 1200 catcatgccc cttatgacct gggctacaca cgtgctacaa tgggaagtac aacgagtcgc 1260 aaagtcgcga ggctaagcta atctcttaaa gcttctctca gttcggattg taggctgcaa 1320 ctcgcctaca tgaagccgga atcgctagta atcgcggatc agcacgccgc ggtgaatacg 1380 ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac ccgaagtcgg 1440 tgaggtaacc ttttggagcc agccgcctaa ggtgggatag atgattgggg tgaagtcgta 1500 acaaggtagc cgtatcggaa ggtgcggctg gatca 1535

<210> 130

<211> 1530

<212> DNA

<213> Prior Sequence 22

<400> 130 cttagagttt gatcctggct caggacgaac gctggcggcg tgcctaatac atgcaagtcg 60 aacgcttttt ttttcaccgg agcttgctcc accgaaagaa aaggagtggc gaacgggtga 120 gtaacacgtg ggtaacctgc ccatcagaag gggataacac ttggaaacag gtgctaatac 180 cgtataacaa tagaaaccgc atggtttcta tttgaaaggc gcttttgcgt cactgatgga 240 tggacccgcg gtgcattagc tagttggtga ggtaacggct caccaaggca acgatgcata 300 gccgacctga gagggtgatc ggccacattg ggactgagac acggcccaaa ctcctacggg 360 aggcagcagt agggaatctt cggcaatgga cgaaagtctg accgagcaac gccgcgtgag 420 tgaagaaggt tttcggatcg taaaactctg ttgttagaga agaacaagga tgagagtaga 480 atgttcatcc cttgacggta tctaaccaga aagccacggc taactacgtg ccagcagccg 540 cggtaatacg taggtggcaa gcgttgtccg gatttattgg gcgtaaagcg agcgcaggcg 600 gtttcttaag tctgatgtga aagcccccgg ctcaaccggg gagggtcatt ggaaactggg 660 aagcttgagt gcagaagagg agagtggaat tccatgtgta gcggtgaaat gcgtagatat 720 atggaggaac accagtggcg aaggcggctc tctggtctgt aactgacgct gaggctcgaa 780 agcgtgggga gcaaacagga ttagataccc cggtagtcca cgccgtaaac gatgagtgct 840 aagtgttgga gggtttccgc ccttcagtgc tgcagctaac gcattaagca ctccgcctgg 900 ggagtacgac cgcaaggttg aaactcaaag gaattgacgg gggcccgcac aagcggtgga 960 gcatgtggtt taattcgaag caacgcgaag aaccttacca ggtcttgaca tcctttgacc 1020 actctagaga tagagcttcc ccttcggggg caaagtgaca ggtggtgcat ggttgtcgtc 1080 agctcgtgtc gtgagatgtt gggttgagtc ccgcaacgag cgcaaccctt attgttagtt 1140 gccatcattt agttgggcac tctagcgaga ctgccggtga caaaccggag gaaggtgggg 1200 atgacgtcaa atcatcatgc cccttatgac ctgggctaca cacgtgctac aatgggaagt 1260 acaacgagtc gcgaagtcgc gaggctaagc taatctctta aagcttctct cagttcggat 1320 tgtaggctgc aactcgccta catgaagccg gaatcgctag taatcgcgga tcagcacgcc 1380 gcggtgaata cgttcccggg ccttgtacac accgcccgtc acaccacgag agtttgtaac 1440 acccgaagtc ggtgaggtaa ccttttggag ccagccgcct aaggtgggat agatgattgg 1500 ggtgaagtcg taacaaggta accgtaaagg 1530

<210> 131 <211> 1513

<212> DNA

<213> Enterococcus pseudoavium

<400> 131 cgcacgaacg ctggcggcgt gcctaataca tgcaagtcga acgctttttc tttcaccgga 60 gcttgctcca ccgaaagaaa aagagtggcg aacgggtgag taacacgtgg gtaacctgcc 120 catcagaagg ggataacact tggaaacagg tgctaatacc gtataacaag taaaaccgca 180 tggttttact ttgaaaggcg cttttgcgtc actgatggat ggacccgcgg tgcattagct 240 agttggtgag gtaacggctc accaaggcaa cgatgcatag ccgacctgag agggtgatcg 300 gccacattgg gactgagaca cggcccaaac tcctacggga ggcagcagta gggaatcttc 360 ggcaatggac gaaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt ttcggatcgt 420 aaaactctgt tgttagagaa gaacaaggat gagagtaaaa tgttcatccc ttgacggtat 480 ctaaccagaa agccacggct aactacgtgc cagcagccgc ggtaatacgt aggtggcaag 540 cgttgtccgg atttattggg cgtaaagcga gcgcaggcgg tttcttaagt ctgatgtgaa 600 agcccccggc tcaaccgggg agggtcattg gaaactggga aacttgagtg cagaagagga 660 gagtggaatt ccatgtgtag cggtgaaatg cgtagatata tggaggaaca ccagtggcga 720 aggcggctct ctggtctgta actgacgctg aggctcgaaa gcgtggggag caaacaggat 780 tagataccct ggtagtccac gccgtaaacg atgagtgcta agtgttggag ggtttccgcc 840 cttcagtgct gcagctaacg cattaagcac tccgcctggg gagtacgacc gcaaggttga 900 aactcaaagg aattgacggg ggcccgcaca agcggtggag catgtggttt aattcgaagc 960 aacgcgaaga accttaccag gtcttgacat cctttgacca ctctagagat agagcttccc 1020 cttcgggggc aaagtgacag gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg 1080 ggttaagtcc cgcaacgagc gcaaccctta ttgttagttg ccatcattta gttgggcact 1140 ctagcgagac tgccggtgac aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc 1200 ccttatgacc tgggctacac acgtgctaca atgggaagta caacgagtcg cgaagtcgcg 1260 aggctaagct aatctcttaa agcttctctc agttcggatt gtaggctgca actcgcctac 1320 atgaagccgg aatcgctagt aatcgcggat cagcacgccg cggtgaatac gttcccgggc 1380 cttgtacaca ccgcccgtca caccacgaga gtttgtaaca cccgaagtcg gtgaggtaac 1440 cttttggagc cagccgccta aggtgggata gatgattggg gtgaagtcgt aacaaggtag 1500 ccgtatcgga agg 1513

<210> 132

<211> 1507

<212> DNA

<213> Enterococcus hirae 3 <400> 132 cgaacgctgg cggcgtgcct aatacatgca agtcgaacgc ttctttttcc accggagctt 60 gctccaccgg aaaaagagga gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc 120 agaaggggat aacacttgga aacaggtgct aataccgtat aacaatcgaa accgcatggt 180 tttgatttga aaggcgcttt cgggtgtcgc tgatggatgg acccgcggtg cattagctag 240 ttggtgaggt aacggctcac caaggcgacg atgcatagcc gacctgagag ggtgatcggc 300 cacattggga ctgagacacg gcccaaactc ctacgggagg cagcagtagg gaatcttcgg 360 caatggacga aagtctgacc gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa 420 aactctgttg ttagagaaga acaaggatga gagtaactgt tcatcccttg acggtatcta 480 accagaaagc cacggctaac tacgtgccag cagccgcggt aatacgtagg tggcaagcgt 540 tgtccggatt tattgggcgt aaagcgagcg caggcggttt cttaagtctg atgtgaaagc 600 ccccggctca accggggagg gtcattggaa actgggagac ttgagtgcag aagaggagag 660 tggaattcca tgtgtagcgg tgaaatgcgt agatatatgg aggaacacca gtggcgaagg 720 cggctctctg gtctgtaact gacgctgagg ctcgaaagcg tggggagcaa acaggattag 780 ataccctggt agtccacgcc gtaaacgatg agtgctaagt gttggagggt ttccgccctt 840 cagtgctgca gctaacgcat taagcactcc gcctggggag tacgaccgca aggttgaaac 900 tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac 960 gcgaagaacc ttaccaggtc ttgacatcct ttgaccactc tagagataga gcttcccctt 1020 cgggggcaaa gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt 1080 taagtcccgc aacgagcgca acccttattg ttagttgcca tcatttagtt gggcactcta 1140 gcaagactgc cggtgacaaa ccggaggaag gtggggatga cgtcaaatca tcatgcccct 1200 tatgacctgg gctacacacg tgctacaatg ggaagtacaa cgagtcgcaa agtcgcgagg 1260 ctaagctaat ctcttaaagc ttctctcagt tcggattgta ggctgcaact cgcctacatg 1320 aagccggaat cgctagtaat cgcggatcag cacgccgcgg tgaatacgtt cccgggcctt 1380 gtacacaccg cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg aggtaacctt 1440 ttggagccag ccgcctaagg tgggatagat gattggggtg aagtcgtaac aaggtagccg 1500 tatcgga 1507

<210> 133

<211> 1500

<212> DNA

<213> Enterococcus viikkiensis

<400> 133 gcggcgtgcc taatacatgc aagtcgaacg ctttttcttt caccggagct tgctccaccg 60 aaagaaaaag agtggcgaac gggtgagtaa cacgtgggta acctgcccat cagaagggga 120 taacacttgg aaacaggtgc taataccgta taacaagtaa aaccgcatgg ttttactttg 180 aaaggcgctt ttgcgtcact gatggatgga cccgcggtgc attagctagt tggtgaggta 240 acggctcacc aaggcaacga tgcatagccg acctgagagg gtgatcggcc acattgggac 300 tgagacacgg cccaaactcc tacgggaggc agcagtaggg aatcttcggc aatggacgaa 360 agtctgaccg agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt 420 tagagaagaa caaggatgag agtaaaatgt tcatcccttg acggtatcta accagaaagc 480 cacggctaac tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt 540 tattgggcgt aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca 600 accggggagg gtcattggaa actgggaaac ttgagtgcag aagaggagag tggaattcca 660 tgtgtagcgg tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg 720 gtctgtaact gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt 780 agtccacgcc gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca 840 gctaacgcat taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat 900 tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc 960 ttaccaggtc ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa 1020 gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc 1080 aacgagcgca acccttattg ttagttgcca tcatttagtt gggcactcta gcgagactgc 1140 cggtgacaaa ccggaggaag gtggggatga cgtcaaatca tcatgcccct tatgacctgg 1200 gctacacacg tgctacaatg ggaagtacaa cgagtcgcga agtcgcgagg ctaagctaat 1260 ctcttaaagc ttctctcagt tcggattgta ggctgcaact cgcctacatg aagccggaat 1320 cgctagtaat cgcggatcag cacgccgcgg tgaatacgtt cccgggcctt gtacacaccg 1380 cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg aggtaacctt ttggagccag 1440 ccgcctaagg tgggatagat gattggggtg aagtcgtaac aaggtagccg tatcggaagg 1500

<210> 134

<211> 1500

<212> DNA

<213> Enterococcus viikkiensis 2

<400> 134 gcggcgtgcc taatacatgc aagtcgaacg ctttttcttt caccggagct tgctccaccg 60 aaagaaaaag agtggcgaac gggtgagtaa cacgtgggta acctgcccat cagaagggga 120 taacacttgg aaacaggtgc taataccgta taacaagtaa aaccgcatgg ttttactttg 180 aaaggcgctt ttgcgtcact gatggatgga cccgcggtgc attagctagt tggtgaggta 240 acggctcacc aaggcaacga tgcatagccg acctgagagg gtgatcggcc acattgggac 300 tgagacacgg cccaaactcc tacgggaggc agcagtaggg aatcttcggc aatggacgaa 360 agtctgaccg agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt 420 tagagaagaa caaggatgag agtaaaatgt tcatcccttg acggtatcta accagaaagc 480 cacggctaac tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt 540 tattgggcgt aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca 600 accggggagg gtcattggaa actgggaaac ttgagtgcag aagaggagag tggaattcca 660 tgtgtagcgg tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg 720 gtctgtaact gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt 780 agtccacgcc gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca 840 gctaacgcat taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat 900 tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc 960 ttaccaggtc ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa 1020 gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc 1080 aacgagcgca acccttattg ttagttgcca tcatttagtt gggcactcta gcgagactgc 1140 cggtgacaaa ccggaggaag gtggggatga cgtcaaatca tcatgcccct tatgacctgg 1200 gctacacacg tgctacaatg ggaagtacaa cgagtcgcga agtcgcgagg ctaagctaat 1260 ctcttaaagc ttctctcagt tcggattgta ggctgcaact cgcctacatg aagccggaat 1320 cgctagtaat cgcggatcag cacgccgcgg tgaatacgtt cccgggcctt gtacacaccg 1380 cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg aggtaacctt ttggagccag 1440 ccgcctaagg tgggatagat gattggggtg aagtcgtaac aaggtagccg tatcggaagg 1500

<210> 135

<211> 1497

<212> DNA

<213> Enterococcus hirae 4

<400> 135 ggacgaacgc tggcggcgtg cctaatacat gcaagtcgaa cgcttctttt tccaccggag 60 cttgctccac cggaaaaaga ggagtggcga acgggtgagt aacacgtggg taacctgccc 120 atcagaaggg gataacactt ggaaacaggt gctaataccg tataacaatc gaaaccgcat 180 ggttttgatt tgaaaggcgc tttcgggtgt cgctgatgga tggacccgcg gtgcattagc 240 tagttggtga ggtaacggct caccaaggcg acgatgcata gccgacctga gagggtgatc 300 ggccacattg ggactgagac acggcccaaa ctcctacggg aggcagcagt agggaatctt 360 cggcaatgga cgaaagtctg accgagcaac gccgcgtgag tgaagaaggt tttcggatcg 420 taaaactctg ttgttagaga agaacaagga tgagagtaac tgttcatccc ttgacggtat 480 ctaaccagaa agccacggct aactacgtgc cagcagccgc ggtaatacgt aggtggcaag 540 cgttgtccgg atttattggg cgtaaagcga gcgcaggcgg tttcttaagt ctgatgtgaa 600 agcccccggc tcaaccgggg agggtcattg gaaactggga gacttgagtg cagaagagga 660 gagtggaatt ccatgtgtag cggtgaaatg cgtagatata tggaggaaca ccagtggcga 720 aggcggctct ctggtctgta actgacgctg aggctcgaaa gcgtggggag caaacaggat 780 tagataccct ggtagtccac gccgtaaacg atgagtgcta agtgttggag ggtttccgcc 840 cttcagtgct gcagctaacg cattaagcac tccgcctggg gagtacgacc gcaaggttga 900 aactcaaagg aattgacggg ggcccgcaca agcggtggag catgtggttt aattcgaagc 960 aacgcgaaga accttaccag gtcttgacat cctttgacca ctctagagat agagcttccc 1020 cttcgggggc aaagtgacag gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg 1080 ggttaagtcc cgcaacgagc gcaaccctta ttgttagttg ccatcattta gttgggcact 1140 ctagcaagac tgccggtgac aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc 1200 ccttatgacc tgggctacac acgtgctaca atgggaagta caacgagtcg caaagtcgcg 1260 aggctaagct aatctcttaa agcttctctc agttcggatt gtaggctgca actcgcctac 1320 atgaagccgg aatcgctagt aatcgcggat cagcacgccg cggtgaatac gttcccgggc 1380 cttgtacaca ccgcccgtca caccacgaga gtttgtaaca cccgaagtcg gtgaggtaac 1440 cttttggagc cagccgccta aggtgggata gatgattggg gtgaagtcgt aacaagg 1497

<210> 136

<211> 1497

<212> DNA

<213> Enterococcus viikkiensis 3

<400> 136 gcggcgtgcc taatacatgc aagtcgaacg ctttttcttt caccggagct tgctccaccg 60 aaagaaaaga gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat 120 aacacttgga aacaggtgct aataccgtat aacaagtaaa accgcatggt tttactttga 180 aaggcgcctt tgcgtcactg atggatggac ccgcggtgca ttagctagtt ggtgaggtaa 240 cggctcacca aggcaacgat gcatagccga cctgagaggg tgatcggcca cattgggact 300 gagacacggc ccaaactcct acgggaggca gcagtaggga atcttcggca atggacgaaa 360 gtctgaccga gcaacgccgc gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt 420 agagaagaac aaggatgaga gtaaaatgtt catcccttga cggtatctaa ccagaaagcc 480 acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggattt 540 attgggcgta aagcgagcgc aggcgtttct taagtctgat gtgaaagccc ccggctcaac 600 cggggagggt cattggaaac tgggaaactt gagtgcagaa gaggagagtg gaattccatg 660 tgtagcggtg aaatgcgtag atatatggag gaacaccagt ggcgaaggcg gctctctggt 720 ctgtaactga cgctgaggct cgaaagcgtg gggagcaaac aggattagat accctggtag 780 tccacgccgt aaacgatgag tgctaagtgt tggagggttt ccgcccttca gtgctgcagc 840 taacgcatta agcactccgc ctggggagta cgaccgcaag ttgaaactca aaggaattga 900 cgggggcccg cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg aagaacctta 960 ccaggtcttg acatcctttg accactctag agatagagct tccccttcgg gggcaaagtg 1020 acaggtggtg catggttgtc gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac 1080 gagcgcaacc cttattgtta gttgccatca tttagttggg cactctagcg agactgccgg 1140 tgacaaaccg gaggaaggtg gggatgacgt caaatcatca tgccccttat gacctgggct 1200 acacacgtgc tacaatggga agtacaacga gtcgcgaagt cgcgaggcta agctaatctc 1260 ttaaagcttc tctcagttcg gattgtaggc tgcaactcgc ctacatgaag ccggaatcgc 1320 tagtaatcgc ggatcagcac gccgcggtga atacgttccc gggccttgta cacaccgccc 1380 gtcacaccac gagagtttgt aacacccgaa gtcggtgagg taaccttttg gagccagccg 1440 cctaaggtgg gatagatgat tggggtgaag tcgtaacaag gtagccgtat cggaagg 1497

<210> 137

<211> 1494

<212> DNA

<213> Enterococcus devriesei

<400> 137 tttgatcctg gctgaggacg aacgctggcg gcgtgcctaa tacatgcaag tcgaacgctt 60 tttctttcac cggagcttgc tccaccgaaa gaaaaagagt ggcgaacggg tgagtaacac 120 gtgggtaacc tgcccatcag aaggggataa cacttggaaa caggtgctaa taccgtataa 180 caagtaaaac cgcatggttt tactttgaaa ggcgcttttg cgtcactgat ggatggaccc 240 gcggtgcatt agctagttgg tgaggtaacg gctcaccaag gcaacgatgc atagccgacc 300 tgagagggtg atcggccaca ttgggactga gacacggccc aaactcctac gggaggcagc 360 agtagggaat cttcggcaat ggacgaaagt ctgaccgagc aacgccgcgt gagtgaagaa 420 ggttttcgga tcgtaaaact ctgttgttag agaagaacaa ggatgagagt aaaaagttca 480 tcccttgacg gtatctaacc agaaagccac ggctaactac gtgccagcag ccgcggtaat 540 acgtaggtgg caagcgttgt ccggatttat tgggcgtaaa gcgagcgcag gcggtttctt 600 aagtctgatg tgaaagcccc cggctcaacc ggggagggtc attggaaact gggaaacttg 660 agtgcagaag aggagagtgg aattccatgt gtagcggtga aatgcgtaga tatatggagg 720 aacaccagtg gcgaaggcgg ctctctggtc tgtaactgac gctgaggctc gaaagcgtgg 780 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgagt gctaagtgtt 840 ggagggtttc cgcccttcag tgctgcagct aacgcattaa gcactccgcc tggggagtac 900 gaccgcaagg ttgaaactca aaggaattga cgggggcccg cacaagcggt ggagcatgtg 960 gtttaattcg aagcaacgcg aagaacctta ccaggtcttg acatcctttg accactctag 1020 agatagagct tccccttcgg gggcaaagtg acaggtggtg catggttgtc gtcagctcgt 1080 gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc cttattgtta gttgccatca 1140 tttagttggg cactctagcg agactgccgg tgacaaaccg gaggaaggtg gggatgacgt 1200 caaatcatca tgccccttat gacctgggct acacacgtgc tacaatggga agtacaacga 1260 gtcgcgaagt cgcgaggcta agctaatctc ttaaagcttc tctcagttcg gattgtaggc 1320 tgcaactcgc ctacatgaag ccggaatcgc tagtaatcgc ggatcagcac gccgcggtga 1380 atacgttccc gggccttgta cacaccgccc gtcacaccac gagagtttgt aacacccgaa 1440 gtcggtgagg taaccttttg gagccagccg cctaaggtgg gatagatgat tggg 1494

<210> 138

<211> 1493

<212> DNA

<213> Enterococcus devriesei 2

<400> 138 tttgatcctg gctgaggacg aacgctggcg gcgtgcctaa tacatgcaag tcgaacgctt 60 tttctttcac cggagcttgc tccaccgaaa gaaaaagagt ggcgaacggg tgagtaacac 120 gtgggtaacc tgcccatcag aaggggataa cacttggaaa caggtgctaa taccgtataa 180 caagtaaaac cgcatggttt tactttgaaa ggcgcttttg cgtcactgat ggatggaccc 240 gcggtgcatt agctagttgg tgaggtaacg gctcaccaag gcaacgatgc atagccgacc 300 tgagagggtg atcggccaca ttgggactga gacacggccc aaactcctac gggaggcagc 360 agtagggaat cttcggcaat ggacgaaagt ctgaccgagc aacgccgcgt gagtgaagaa 420 ggttttcgga tcgtaaaact ctgttgttag agaagaacaa ggatgagagt aaaaagttca 480 tcccttgacg gtatctaacc agaaagccac ggctaactac gtgccagcag ccgcggtaat 540 acgtaggtgg caagcgttgt ccggatttat tgggcgtaaa gcgagcgcag gcggtttctt 600 aagtctgatg tgaaagcccc cggctcaacc ggggagggtc attggaaact gggaaacttg 660 agtgcagaag aggagagtgg aattccatgt gtagcggtga aatgcgtaga tatatggagg 720 aacaccagtg gcgaaggcgg ctctctggtc tgtaactgac gctgaggctc gaaagcgtgg 780 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgagt gctaagtgtt 840 ggagggtttc cgcccttcag tgctgcagct aacgcattaa gcactccgcc tggggagtac 900 gaccgcaagg ttgaaactca aaggaattga cgggggcccg cacaagcggt ggagcatgtg 960 gtttaattcg aagcaacgcg aagaacctta ccaggtcttg acatcctttg accactctag 1020 agatagagct tccccttcgg gggcaaagtg acaggtggtg catggttgtc gtcagctcgt 1080 gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc cttattgtta gttgccatca 1140 tttagttggg cactctagcg agactgccgg tgacaaaccg gaggaaggtg gggatgacgt 1200 caaatcatca tgccccttat gacctgggct acacacgtgc tacaatggga agtacaacga 1260 gtcgcgaagt cgcgaggcta agctaatctc ttaaagcttc tctcagttcg gattgtaggc 1320 tgcaactcgc ctacatgaag ccggaatcgc tagtaatcgc ggatcagcac gccgcggtga 1380 atacgttccc gggccttgta cacaccgccc gtcacaccac gagagtttgt aacacccgaa 1440 gtcggtgagg taaccttttg gagccagccg cctaaggtgg gatagatgat tgg 1493

<210> 139

<211> 1490

<212> DNA

<213> Enterococcus devriesei 3

<400> 139 gatcctggct gaggacgaac gctggcggcg tgcctaatac atgcaagtcg aacgcttttt 60 ctttcaccgg agcttgctcc accgaaagaa aaagagtggc gaacgggtga gtaacacgtg 120 ggtaacctgc ccatcagaag gggataacac ttggaaacag gtgctaatac cgtataacaa 180 gtaaaaccgc atggttttac tttgaaaggc gcttttgcgt cactgatgga tggacccgcg 240 gtgcattagc tagttggtga ggtaacggct caccaaggca acgatgcata gccgacctga 300 gagggtgatc ggccacattg ggactgagac acggcccaaa ctcctacggg aggcagcagt 360 agggaatctt cggcaatgga cgaaagtctg accgagcaac gccgcgtgag tgaagaaggt 420 tttcggatcg taaaactctg ttgttagaga agaacaagga tgagagtaaa atgttcatcc 480 cttgacggta tctaaccaga aagccacggc taactacgtg ccagcagccg cggtaatacg 540 taggtggcaa gcgttgtccg gatttattgg gcgtaaagcg agcgcaggcg ktttcttaag 600 tctgatgtga aagcccccgg ctcaaccggg gagggtcatt ggaaactggg aaacttgagt 660 gcagaagagg agagtggaat tccatgtgta gcggtgaaat gcgtagatat atggaggaac 720 accagtggcg aaggcggctc tctggtctgt aactgacgct gaggctcgaa agcgtgggga 780 gcaaacagga ttagataccc tggtagtcca cgccgtaaac gatgagtgct aagtgttgga 840 gggtttccgc ccttcagtgc tgcagctaac gcattaagca ctccgcctgg ggagtacgac 900 cgcaaggttg aaactcaaag gaattgacgg gggcccgcac aagcggtgga gcatgtggtt 960 taattcgaag caacgcgaag aaccttacca ggtcttgaca tcctttgacc actctagaga 1020 tagagcttcc ccttcggggg caaagtgaca ggtggtgcat ggttgtcgtc agctcgtgtc 1080 gtgagatgtt gggttaagtc ccgcaacgag cgcaaccctt attgttagtt gccatcattt 1140 agttgggcac tctagcgaga ctgccggtga caaaccggag gaaggtgggg atgacgtcaa 1200 atcatcatgc cccttatgac ctgggctaca cacgtgctac aatgggaagt acaacgagtc 1260 gcgaagtcgc gaggctaagc taatctctta aagcttctct cagttcggat tgtaggctgc 1320 aactcgccta catgaagccg gaatcgctag taatcgcgga tcagcacgcc gcggtgaata 1380 cgttcccggg ccttgtacac accgcccgtc acaccacgag agtttgtaac acccgaagtc 1440 ggtgaggtaa ccttttggag ccagccgcct aaggtgggat agatgattgg 1490

<210> 140

<211> 1485

<212> DNA

<213> Enterococcus thailandicus

<400> 140 tatacatgca agtcgtacgc tttttctttc accggagctt gctccaccga aagaaaagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggccacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cgactctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 900 ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960 ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa gtgacaggtg 1020 gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca 1080 acccttattg ttagttgcca tcatttagtt gggcactcta gcaagactgc cggtgacaaa 1140 ccggaggaag gtggggatga cgtcaaatca tcatgcccct tatgacctgg gctacacacg 1200 tgctacaatg ggaagtacaa cgagtcgcga agtcgcgagg ctaagctaat ctcttaaagc 1260 ttctctcagt tcggattgta ggctgcaact cgcctacatg aagccggaat cgctagtaat 1320 cgcggatcag cacgccgcgg tgaatacgtt cccgggcctt gtacacaccg cccgtcacac 1380 cacgaaagtt tgtaacaccc gaagtcggtg aggtaacctt tttggagcca gccgccctaa 1440 ggtgggataa atgattgggg tgaagtccta acaaggtaac cgtaa 1485

<210> 141

<211> 1483

<212> DNA

<213> Prior Sequence 23

<400> 141 gacgaacgct ggcggcgtgc ctaatacatg caagtcgtac gctttttctt tcaccggagc 60 ttgctccacc gaaagaaaag gagtggcgaa cgggtgagta acacgtgggt aacctgccca 120 tcagaagggg ataacacttg gaaacaggtg ctaataccgt ataacaatcg aaaccgcatg 180 gttttgattt gaaaggcgct ttcgggtgtc gctgatggat ggacccgcgg tgcattagct 240 agttggtgag gtaacggctc accaaggcca cgatgcatag ccgacctgag agggtgatcg 300 gccacattgg gactgagaca cggcccaaac tcctacggga ggcagcagta gggaatcttc 360 ggcaatggac gaaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt ttcggatcgt 420 aaaactctgt tgttagagga gaacaaggat gagagtaact gttcatccct tgacggtatc 480 taaccagaaa gccacggcta actacgtgcc agcagccgcg gtaatacgta ggtggcaagc 540 gttgtccgga tttattgggc gtaaagcgag cgcaggcggt ttcttaagtc tgatgtgaaa 600 gcccccggct caaccgggga gggtcattgg aaactgggag acttgagtgc agaagaggag 660 agtggaattc catgtgtagc ggtgaaatgc gtagatatat ggaggaacac cagtggcgaa 720 ggcgactctc tggtctgtaa ctgacgctga ggctcgaaag cgtggggagc aaacaggatt 780 agataccctg gtagtccacg ccgtaaacga tgagtgctaa gtgttggagg gtttccgccc 840 ttcagtgctg cagctaacgc attaagcact ccgcctgggg agtacgaccg caaggttgaa 900 acccaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca 960 acgcgaagaa ccttaccagg tcttgacatc ctttgaccac tctagagata gagcttcccc 1020 ttcgggggca aagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg 1080 gttaagtccc gcaacgagcg caacccttat tgttagttgc catcatttag ttgggcactc 1140 tagcaagact gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc 1200 cttatgacct gggctacaca cgtgctacaa tgggaagtac aacgagtcgc gaagtcgcga 1260 ggctaagcta atctcttaaa gcttctctca gttcggattg taggctgcaa ctcgcctaca 1320 tgaagccgga atcgctagta atcgcggatc agcacgccgc ggtgaatacg ttcccgggcc 1380 ttgtacacac cgcccgtcac accacgaaag tttgtaacac ccgaagtcgg tgaggtaacc 1440 tttttggagc cagccgccta aggtgggata gatgattggg gtg 1483 <210> 142

<211> 1483

<212> DNA

<213> Prior Sequence 24

<400> 142 gacgaacgct ggcggcgtgc ctaatacatg caagtcgtac gctttttctt tcaccggagc 60 ttgctccacc gaaagaaaag gagtggcgaa cgggtgagta acacgtgggt aacctgccca 120 tcagaagggg ataacacttg gaaacaggtg ctaataccgt ataacaatcg aaaccgcatg 180 gttttgattt gaaaggcgct ttcgggtgtc gctgatggat ggacccgcgg tgcattagct 240 agttggtgag gtaacggctc accaaggcca cgatgcatag ccgacctgag agggtgatcg 300 gccacattgg gactgagaca cggcccaaac tcctacggga ggcagcagta gggaatcttc 360 ggcaatggac gaaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt ttcggatcgt 420 aaaactctgt tgttagagaa gaacaaggat gagagtaact gttcatccct tgacggtatc 480 taaccagaaa gccacggcta actacgtgcc agcagccgcg gtaatacgta ggtggcaagc 540 gttgtccgga tttattgggc gtaaagcgag cgcaggcggt ttcttaagtc tgatgtgaaa 600 gcccccggct caaccgggga gggtcattgg aaactgggag acttgagtgc agaagaggag 660 agtggaattc catgtgtagc ggtgaaatgc gtagatatat ggaggaacac cagtggcgaa 720 ggcgactctc tggtctgtaa ctgacgctga ggctcgaaag cgtggggagc aaacaggatt 780 agataccctg gtagtccacg ccgtaaacga tgagtgctaa gtgttggagg gtttccgccc 840 ttcagtgctg cagctaacgc attaagcact ccgcctgggg agtacgaccg caaggttgaa 900 actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca 960 acgcgaagaa ccttaccagg tcttgacatc ctttgaccac tctagagata gagcttcccc 1020 ttcgggggca aagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg 1080 gttaagtccc gcaacgagcg caacccttat tgttagttgc catcatttag ttgggcactc 1140 tagcaagact gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc 1200 cttatgacct gggctacaca cgtgctacaa tgggaagtac aacgagtcgc gaagtcgcga 1260 ggctaagcta atctcttaaa gcttctctca gtccggattg taggctgcaa ctcgcctacg 1320 tgaagccgga atcgctagta atcgcggatc agcacgccgc ggtgaatacg ttcccgggcc 1380 ttgtacacac cgcccgtcac accacgaaag tttgtaacac ccgaagtcgg tgaggtaacc 1440 tttttggagc cagccgccta aggtgggata gatgattggg gtg 1483

<210> 143

<211> 1483

<212> DNA

<213> Enterococcus thailandicus 2

<400> 143 gacgaacgct ggcggcgtgc ctaatacatg caagtcgtac gctttttctt tcaccggagc 60 ttgctccacc gaaagaaaag gagtggcgaa cgggtgagta acacgtgggt aacctgccca 120 tcagaagggg ataacacttg gaaacaggtg ctaataccgt ataacaatcg aaaccgcatg 180 gttttgattt gaaaggcgct ttcgggtgtc gctgatggat ggacccgcgg tgcattagct 240 agttggtgag gtaacggctc accaaggcca cgatgcatag ccgacctgag agggtgatcg 300 gccacattgg gactgagaca cggcccaaac tcctacggga ggcagcagta gggaatcttc 360 ggcaatggac gaaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt ttcggatcgt 420 aaaactctgt tgttagagaa gaacaaggat gagagtaact gttcatccct tgacggtatc 480 taaccagaaa gccacggcta actacgtgcc agcagccgcg gtaatacgta ggtggcaagc 540 gttgtccgga tttattgggc gtaaagcgag cgcaggcggt ttcttaagtc tgatgtgaaa 600 gcccccggct caaccgggga gggtcattgg aaactgggag acttgagtgc agaagaggag 660 agtggaattc catgtgtagc ggtgaaatgc gtagatatat ggaggaacac cagtggcgaa 720 ggcgactctc tggtctgyaa ctgacgctga ggctcgaaag cgtggggagc aaacaggatt 780 agataccctg gtagtccacg ccgtaaacga tgagtgctaa gtgttggagg gtttccgccc 840 ttcagtgctg cagctaacgc attaagcact ccgcctgggg agtacgaccg caaggttgaa 900 actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca 960 acgcgaagaa ccttaccagg tcttgacatc ctttgaccac tctagagata gagcttcccc 1020 ttcgggggca aagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg 1080 gttaagtccc gcaacgagcg caacccttat tgttagttgc catcatttag ttgggcactc 1140 tagcaagact gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc 1200 cttatgacct gggctacaca cgtgctacaa tgggaagtac aacgagtcgc gaagtcgcga 1260 ggctaagcta atctcttaaa gcttctctca gttcggattg taggctgcaa ctcgcctaca 1320 tgaagccgga atcgctagta atcgcggatc agcacgccgc ggtgaatacg ttcccgggcc 1380 ttgtacacac cgcccgtcac accacgaaag tttgtaacac ccgaagtcgg tgaggtaacc 1440 tttttggagc cagccgccta aggtgggata gatgattggg gtg 1483

<210> 144

<211> 1482

<212> DNA

<213> Enterococcus hawaiiensis

<400> 144 gacgaacgct ggcggcgtgc ctaatacatg caagtcgaac gctttttctt tcaccggagc 60 ttgctccacc gaaagaaaag gagtggcgaa cgggtgagta acacgtgggt aacctgccca 120 tcagaagggg ataacacttg gaaacaggtg ctaataccgt ataataagta aaaccgcatg 180 gttttacttt gaaaggcgct tttgcgtcac tgatggatgg acccgcggtg cattagctag 240 ttggtgaggt aacggctcac caaggcaacg atgcatagcc gacctgagag ggtgatcggc 300 cacattggga ctgagacacg gcccaaactc ctacgggagg cagcagtagg gaatcttcgg 360 caatggacga aagtctgacc gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa 420 aactctgttg ttagagaaga acaaggatga gagtaaaacg ttcatccctt gacggtatct 480 aaccagaaag ccacggctaa ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg 540 ttgtccggat ttattgggcg taaagcgagc gcaggcggtt tcttaagtct gatgtgaaag 600 cccccggctc aaccggggag ggtcattgga aactgggaaa cttgagtgca gaagaggaga 660 gtggaattcc atgtgtagcg gtgaaatgcg tagatatatg gaggaacacc agtggcgaag 720 gcggctctct ggtctgtaac tgacgctgag gctcgaaagc gtggggagca aacaggatta 780 gataccctgg tagtccacgc cgtaaacgat gagtgctaag tgttggaggg tttccgccct 840 tcagtgctgc agctaacgca ttaagcactc cgcctgggga gtacgaccgc aaggttgaaa 900 ctcaaaggaa ttgacggggg cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa 960 cgcgaagaac cttaccaggt cttgacatcc tttgaccact ctagagatag agcttcccct 1020 tcgggggcaa agtgacaggt ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg 1080 ttaagtcccg caacgagcgc aacccttatt gttagttgcc atcatttagt tgggcactct 1140 agcgagactg ccggtgacaa accggaggaa ggtggggatg acgtcaaatc atcatgcccc 1200 ttatgacctg ggctacacac gtgctacaat gggaagtaca acgagtcgcg aagtcgcgag 1260 gctaagctaa tctcttaaag cttctctcag ttcggattgt aggctgcaac tcgcctacat 1320 gaagccggaa tcgctagtaa tcgcggatca gcacgccgcg gtgaatacgt tcccgggcct 1380 tgtacacacc gcccgtcaca ccacgagagt ttgtaacacc cgaagtcggt gaggtaacct 1440 ttttggagcc agccgcctaa ggtgggatag atgattgggg tg 1482

<210> 145

<211> 1481

<212> DNA

<213> Enterococcus sp. 4

<400> 145 caccccaatc atctatccca ccttaggcgg ctggctccaa aggttacctc accgacttcg 60 ggtgttacaa actttcgtgg tgtgacgggc ggtgtgtaca aggcccggga acgtattcac 120 cgcggcgtgc tgatccgcga ttactagcga ttccggcttc atgtaggcga gttgcagcct 180 acaatccgaa ctgagagaag ctttaagaga ttagcttagc ctcgcgactt cgcgactcgt 240 tgtacttccc attgtagcac gtgtgtagcc caggtcataa ggggcatgat gatttgacgt 300 catccccacc ttcctccggt ttgtcaccgg cagtcttgct agagtgccca acttaatgat 360 ggcaactaac aataagggtt gcgctcgttg cgggacttaa cccaacatct cacgacacga 420 gctgacgaca accatgcacc acctgtcact ttgcccccga aggggaagct ctatctctag 480 agtggtcaaa ggatgtcaag acctggtaag gttcttcgcg ttgcttcgaa ttaaaccaca 540 tgctccaccg cttgtgcggg cccccgtcaa ttcctttgag tttcaacctt gcggtcgtac 600 tccccaggcg gagtgcttaa tgcgttagct gcagcactga agggcggaaa ccctccaaca 660 cttagcactc atcgtttacg gcgtggacta ccagggtatc taatcctgtt tgctccccac 720 gctttcgagc ctcagcgtca gttacagacc agagagtcgc cttcgccact ggtgttcctc 780 catatatcta cgcatttcac cgctacacat ggaattccac tctcctcttc tgcactcaag 840 tctcccagtt tccaatgacc ctccccggtt gagccggggg ctttcacatc agacttaaga 900 aaccgcctgc gctcgcttta cgcccaataa atccggacaa cgcttgccac ctacgtatta 960 ccgcggctgc tggcacgtag ttagccgtgg ctttctggtt agataccgtc aagggatgaa 1020 cagttactct catccttgtt cttctctaac aacagagttt tacgatccga aaaccttctt 1080 cactcacgcg gcgttgctcg gtcagacttt cgtccattgc cgaagattcc ctactgctgc 1140 ctcccgtagg agtttgggcc gtgtctcagt cccaatgtgg ccgatcaccc tctcaggtcg 1200 gctatgcatc gtggccttgg tgagccgtta cctcaccaac tagctaatgc accgcgggtc 1260 catccatcag cgacacccga aagcgccttt caaatcaaaa ccatgcggtt tcgattgtta 1320 tacggtatta gcacctgttt ccaagtgtta tccccttctg atgggcaggt tacccacgtg 1380 ttactcaccc gttcgccact ccttttcttt cggtggagca agctccggag aaagaaaaag 1440 cgtacgactt gcatgtatta ggcacgccgc cagcgttcgt c 1481

<210> 146

<211> 1477

<212> DNA

<213> Enterococcus hirae 5

<400> 146 atgcaagtcg aacgcttctt tttccaccgg agcttgctcc accggaaaaa gaggagtggc 60 gaacgggtga gtaacacgtg ggtaacctgc ccatcagaag gggataacac ttggaaacag 120 gtgctaatac cgtataacaa tcgaaaccgc atggttttga tttgaaaggc gctttcgggt 180 gtcgctgatg gatggacccg cggtgcatta gctagttggt gaggtaacgg ctcaccaagg 240 cgacgatgca tagccgacct gagagggtga tcggccacat tgggactgag acacggccca 300 aactcctacg ggaggcagca gtagggaatc ttcggcaatg gacgaaagtc tgaccgagca 360 acgccgcgtg agtgaagaag gttttcggat cgtaaaactc tgttgttaga gaagaacaag 420 gatgagagta actgttcatc ccttgacggt atctaaccag aaagccacgg ctaactacgt 480 gccagcagcc gcggtaatac gtaggtggca agcgttgtcc ggatttattg ggcgtaaagc 540 gagcgcaggc ggtttcttaa gtctgatgtg aaagcccccg gctcaaccgg ggagggtcat 600 tggaaactgg gagacttgag tgcagaagag gagagtggaa ttccatgtgt agcggtgaaa 660 tgcgtagata tatggaggaa caccagtggc gaaggcggct ctctggtctg taactgacgc 720 tgaggctcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa 780 cgatgagtgc taagtgttgg agggtttccg cccttcagtg ctgcagctaa cgcattaagc 840 actccgcctg gggagtacga ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca 900 caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac 960 atcctttgac cactctagag atagagcttc cccttcgggg gcaaagtgac aggtggtgca 1020 tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct 1080 tattgttagt tgccatcatt tagttgggca ctctagcaag actgccggtg acaaaccgga 1140 ggaaggtggg gatgacgtca aatcatcatg ccccttatga cctgggctac acacgtgcta 1200 caatgggaag tacaacgagt cgcaaagtcg cgaggctaag ctaatctctt aaagcttctc 1260 tcagttcgga ttgtaggctg caactcgcct acatgaagcc ggaatcgcta gtaatcgcgg 1320 atcagcacgc cgcggtgaat acgttcccgg gccttgtaca caccgcccgt cacaccacga 1380 gagtttgtaa cacccgaagt cggtgaggta accttttgga gccagccgcc taaggtggga 1440 tagatgattg gggtgaagtc gtaacaaggt agccgta 1477

<210> 147

<211> 1473

<212> DNA

<213> Enterococcus hirae 6

<400> 147 gagtttgatc ctggctcaga acgaacgctg gcggcgtgct taacacatgc aagtcgaacg 60 cttctttttc caccggagct tgctccaccg gaaaaagagg agtggcgaac gggtgagtaa 120 cacgtgggta acctgcccat cagaagggga taacacttgg aaacaggtgc taataccgta 180 taacaatcga aaccgcatgg ttttgatttg aaaggcgctt tcgggtgtcg ctgatggatg 240 gacccgcggt gcattagcta gttggtgagg taacggctca ccaaggcgac gatgcatagc 300 cgacctgaga gggtgatcgg ccacattggg actgagacac ggcccaaact cctacgggag 360 gcagcagtag ggaatcttcg gcaatggacg aaagtctgac cgagcaacgc cgcgtgagtg 420 aagaaggttt tcggatcgta aaactctgtt gttagagaag aacaaggatg agagtaactg 480 ttcatccctt gacggtatct aaccagaaag ccacggctaa ctacgtgcca gcagccgcgg 540 taatacgtag gtggcaagcg ttgtccggat ttattgggcg taaagcgagc gcaggcggtt 600 tcttaagtct gatgtgaaag cccccggctc aaccggggag ggtcattgga aactgggaga 660 cttgagtgca gaagaggaga gtggaattcc atgtgtagcg gtgaaatgcg tagatatatg 720 gaggaacacc agtggcgaag gcggctctct ggtctgtaac tgacgctgag gctcgaaagc 780 gtggggagca aacaggatta gataccctgg tagtccacgc cgtaaacgat gagtgctaag 840 tgttggaggg tttccgccct tcagtgctgc agctaacgca ttaagcactc cgcctgggga 900 gtacgaccgc aaggttgaaa ctcaaaggaa ttgacggggg cccgcacaag cggtggagca 960 tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt cttgacatcc tttgaccact 1020 ctagagatag agcttcccct tcgggggcaa agtgacaggt ggtgcatggt tgtcgtcagc 1080 tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccttatt gttagttgcc 1140 atcatttagt tgggcactct agcaagactg ccggtgacaa accggaggaa ggtggggatg 1200 acgtcaaatc atcatgcccc ttatgacctg ggctacacac gtgctacaat gggaagtaca 1260 acgagtcgca aagtcgcgag gctaagctaa tctcttaaag cttctctcag ttcggattgt 1320 aggctgcaac tcgcctacat gaagccggaa tcgctagtaa tcgcggatca gcacgccgcg 1380 gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccacgagagt ttgtaacacc 1440 cgaagtcggt gaggtaacct ttggagccag ccg 1473

<210> 148

<211> 1473

<212> DNA

<213> Enterococcus viikkiensis 4

<400> 148 gcggcgtgcc taatacatgc aagtcgaacg ctttttcttt caccggagct tgctccaccg 60 aaagaaaaag agtggcgaac gggtgagtaa cacgtgggta acctgcccat cagaagggga 120 taacacttgg aaacaggtgc taataccgta taacaagtaa aaccgcatgg ttttactttg 180 aaaggcgctt ttgcgtcact gatggatgga cccgcggtgc attagctagt tggtgaggta 240 acggctcacc aaggcaacga tgcatagccg acctgagagg gtgatcggcc acattgggac 300 tgagacacgg cccaaactcc tacgggaggc agcagtaggg aatcttcggc aatggacgaa 360 agtctgaccg agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt 420 tagagaagaa caaggatgag agtaaaatgt tcatcccttg acggtatcta accagaaagc 480 cacggctaac tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt 540 tattgggcgt aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca 600 accggggagg gtcattggaa actgggaaac ttgagtgcag aagaggagag tggaattcca 660 tgtgtagcgg tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg 720 gtctgtaact gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt 780 agtccacgcc gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca 840 gctaacgcat taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat 900 tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc 960 ttaccaggtc ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa 1020 gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc 1080 aacgagcgca acccttattg ttagttgcca tcatttagtt gggcactcta gcgagactgc 1140 cggtgacaaa ccggaggaag gtggggatga cgtcaaatca tcatgcccct tatgacctgg 1200 gctacacacg tgctacaatg ggaagtacaa cgagtcgcga agtcgcgagg ctaagctaat 1260 ctcttaaagc ttctctcagt tcggattgta ggctgcaact cgcctacatg aagccggaat 1320 cgctagtaat cgcggatcag cacgccgcgg tgaatacgtt cccgggcctt gtacacaccg 1380 cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg aggtaacctt ttggagccag 1440 ccgcctaagg tgggatagat gattggggtg aag 1473

<210> 149

<211> 1464

<212> DNA

<213> Enterococcus raffinosus 5

<400> 149 agcgagtgcg cttgctatac atgcagtcga acgctttttc tttcaccgga gcttgctcca 60 ccgaaagaag aggagtggcg aacgggtgag taacacgtgg gtaacctgcc catcagaagg 120 ggataacact tggaaacagg tgctaatacc gtataacagt agaaaccgca tggtttctat 180 ttgaaaggcg cttttgcgtc actgatggat ggacccgcgg tgcattagct agttggtgag 240 gtaacggctc accaaggcaa cgatgcatag ccgacctgag agggtgatcg gccacattgg 300 gactgagaca cggcccaaac tcctacggga ggcagcagta gggaatcttc ggcaatggac 360 gaaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt ttcggatcgt aaaactctgt 420 tgttagagaa gaacaaggat gagaataaaa cgttcatccc ttgacggtat ctaaccagaa 480 agccacggct aactacgtgc cagcagccgc ggtaatacgt aggtggcaag cgttgtccgg 540 atttattggg cgtaaagcga gcgcaggcgg tttcttaagt ctgatgtgaa agcccccggc 600 tcaaccgggg agggtcattg gaaactggga aacttgagtg cagaagagga gagtggaatt 660 ccatgtgtag cggtgaaatg cgtagatata tggaggaaca ccagtggcga aggcggctct 720 ctggtctgta actgacgctg aggctcgaaa gcgtgggagc aaacaggatt agataccctg 780 gtagtccacg ccgtaaacga tgagtgctaa gtgttggagg gtttccgccc ttcagtgctg 840 cagctaacgc attaagcact ccgcctgggg agtacgaccg caaggttgaa actcaaagga 900 attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgaagaa 960 ccttaccagg tcttgacatc ctttgaccac tctagagata gagcttcccc ttcgggggca 1020 aagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc 1080 gcaacgagcg caacccttat tgttagttgc catcatttag ttgggcactc tagcgagact 1140 gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct 1200 gggctacaca cgtgctacaa tgggaagtac aacgagtcgc gaagtcgcga ggctaagcta 1260 atctcttaaa gcttctctca gttcggattg taggctgcaa ctcgcctaca tgaagccgga 1320 atcgctagta atcgcggatc agcacgccgc ggtgaatacg ttcccgggcc ttgtacacac 1380 cgcccgtcac accacgagag tttgtaacac ccgaagtcgg tgaggtaacc ttttggagcc 1440 agccgcctaa ggtgatagta ttaa 1464

<210> 150

<211> 1462

<212> DNA

<213> Enterococcus gilvus 3

<400> 150 gcggcgtgcc taatacatgc aagtcgacgc tttttctttc accggagctt gctccaccga 60 aagaaaagga gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat 120 aacacttgga aacaggtgct aataccgtat aacaatagaa accgcatggt ttctatttga 180 aaggcgcttt tgcgtcactg atggatggac ccgcggtgca ttagctagtt ggtgaggtaa 240 cggctcacca aggcaacgat gcatagccga cctgagaggg tgatcggcca cattgggact 300 gagacacggc ccaaactcct acgggaggca gcagtaggga atcttcggca atggacgaaa 360 gtctgaccga gcaacgccgc gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt 420 agagaagaac aaggatgaga gtaaaatgtt catcccttga cggtatctaa ccagaaagcc 480 acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggattt 540 attgggcgta aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa 600 ccggggaggg tcattggaaa ctgggaaact tgagtgcaga agaggagagt ggaattccat 660 gtgtagcggt kaaatgcgta gatatatgga ggaacaccag tggcgaaggc ggctctctgg 720 tctgtaactg acgctgaggc tcgaaagcgt ggggagcaaa caggattaga taccctggta 780 gtccacgccg taaacgatga gtgctaagtg ttggagggtt tccgcccttc agtgctgcag 840 taacgcatta agcactccgc ctggggagta cgaccgcaag gttgaaactc aaaggaattg 900 acgggggccc gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc gaagaacctt 960 accaggtctt gacatccttt gaccactcta gagatagagc ttccccttcg ggggcaaagt 1020 gacaggtggt gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa 1080 cgagcgcaac ccttattgtt agttgccatc atttagttgg gcactctagc gagactgccg 1140 gtgacaaacc ggaggaaggt ggggatgacg tcaaatcatc atgcccctta tgacctgggc 1200 tacacacgtg ctacaatggg aagtacaacg agtcgcgaag tcgcgaggct aagctaatct 1260 cttaaagctt ctctcagttc ggattgtagg ctgcaactcg cctacatgaa gccggaatcg 1320 ctagtaatcg cggatcagca cgccgcggtg aatacgttcc cgggccttgt acacaccgcc 1380 cgtcacacca cgagagtttg taacacccga agtcggtgag gtaacctttt ggagccagcc 1440 gcctaaggtg ggatagatga tt 1462

<210> 151

<211> 1460

<212> DNA

<213> Enterococcus pseudoavium 2

<220>

<221 > misc_feature

<222> (152).. (152)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (171).. (171 )

<223> n is a, c, g, or t

<400> 151 ggctcaggac gaagctggcg gcgtgcctaa tacatgcaag tcgaacgctt tttctttcac 60 cggagcttgc tccaccgtaa agaaaaagag tggcgaacgg gtgtagtaac acgtgggtaa 120 cctgcccatc agaaggggat aacacttgcg knracaggtg ctaataccag ntataacaag 180 taaaaccgca tggttttact ttgaaaggcg cttttgcgtc actgatggat ggacccgcgg 240 tgcattagct agttggtgag gtaacggctc accaaggcaa cgatgcatag ccgacctgag 300 agggtgatcg gccacattgg gactgagaca cggcccaaac tcctacggga ggcagcagta 360 gggaatcttc ggcaatggac gaaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt 420 ttcggatcgt aaaactctgt tgttagagaa gaacaaggat gagagtaaaa tgttcatccc 480 ttgacggtat ctaaccagaa agccacggct aactacgtgc cagcagccgc ggtaatacgt 540 aggtggcaag cgttgtccgg atttattggg cgtaaagcga gcgcaggcgg tttcttaagt 600 ctgatgtgaa agcccccggc tcaaccgggg agggtcattg gaaactggga aacttgagtg 660 cagaagagga gagtggaatt ccatgtgtag cggtgaaatg cgtagatata tggaggaaca 720 ccagtggcga aggcggctct ctggtctgta actgacgctg aggctcgaaa gcgtggggag 780 caaacaggat tagataccct ggtagtccac gccgtaaacg atgagtgcta agtgttggag 840 ggtttccgcc cttcagtgct gcagctaacg cattaagcac tccgcctggg gagtacgacc 900 gcaaggttga aactcaaagg aattgacggg ggcccgcaca agcggtggag catgtggttt 960 aattcgaagc aacgcgaaga accttaccag gtcttgacat cctttgacca ctctagagat 1020 agagcttccc cttcgggggc aaagtgacag gtggtgcatg gttgtcgtca gctcgtgtcg 1080 tgagatgttg ggttaagtcc cgcaacgagc gcaaccctta ttgttagttg ccatcattta 1140 gttgggcact ctagcgagac tgccggtgac aaaccggagg aaggtgggga tgacgtcaaa 1200 tcatcatgcc ccttatgacc tgggctacac acgtgctaca atgggaagta caacgagtcg 1260 cgaagtcgcg aggctaagct aatctcttaa agcttctctc agttcggatt gtaggctgca 1320 actcgcctac atgaagccgg aatcgctagt aatcgcggat cagcacgccg cggtgaatac 1380 gttcccgggc cttgtacaca ccgcccgtca caccacgaga gtttgtaaca cccgaagtcg 1440 gtgaggtaac cttttggagc 1460

<210> 152

<211> 1455

<212> DNA

<213> Enterococcus gilvus 4

<400> 152 tacatgcaag tcgaacgctt tttctttcac cggagcttgc tccaccgaaa gaaaaggagt 60 ggcgaacggg tgagtaacac gtgggtaacc tgcccatcag aaggggataa cacttggaaa 120 caggtgctaa taccgtataa caatagaaac cgcatggttt ctatttgaaa ggcgcttttg 180 cgtcactgat ggatggaccc gcggtgcatt agctagttgg tgaggtaacg gctcaccaag 240 gcaacgatgc atagccgacc tgagagggtg atcggccaca ttgggactga gacacggccc 300 aaactcctac gggaggcagc agtagggaat cttcggcaat ggacgaaagt ctgaccgagc 360 aacgccgcgt gagtgaagaa ggttttcgga tcgtaaaact ctgttgttag agaagaacaa 420 ggatgagagt aaaatgttca tcccttgacg gtatctaacc agaaagccac ggctaactac 480 gtgccagcag ccgcggtaat acgtaggtgg caagcgttgt ccggatttat tgggcgtaaa 540 gcgagcgcag gcgtttctta agtctgatgt gaaagccccc ggctcaaccg gggagggtca 600 ttggaaactg ggaaacttga gtgcagaaga ggagagtgga attccatgtg tagcggtgaa 660 atgcgtagat atatggagga acaccagtgg cgaaggcggc tctctggtct gtaactgacg 720 ctgaggctcg aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa 780 acgatgagtg ctaagtgttg gagggtttcc gcccttcagt gctgcagcta acgcattaag 840 cactccgcct ggggagtacg accgcaaggt tgaaactcaa aggaattgac gggggcccgc 900 acaagcggtg gagcatgtgg tttaattcga agcaacgcga agaaccttac caggtcttga 960 catcctttga ccactctagg agataggagc ttccccttcg ggggcaaagt gacaggtggt 1020 gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac 1080 ccttattgtt agttgccatc atttagttgg gcactctagc gagactgccg gtgacaaacc 1140 ggaggaaggt ggggatgacg tcaaatcatc atgcccctta tgacctgggc tacacacgtg 1200 ctacaatggg aagtacaacg agtcgcgaag tcgcgaggct aagctaatct cttaaagctt 1260 ctctcagttc ggattgtagg ctgcaactcg cctacatgaa gccggaatcg ctagtaatcg 1320 cggatcagca cgccgcggtg aatacgttcc cgggccttgt acacaccgcc cgtcacacca 1380 cgagagtttg taacacccga agtcggtgag gtaacctttt ggagccagcc gcctaaggtg 1440 ggatagatga ttggg 1455

<210> 153

<211> 1449

<212> DNA

<213> Enterococcus durans

<400> 153 gtygtacgct tctttttcca tcggagcttg ctcaycggga aaagaagagt ggcgaacggg 60 tgagtaacac gtgggtaacc tgcccatcag aaggggataa cacttggaaa caggtgctaa 120 taccgtataa caatcgaaac cgcatggttt tgatttgaaa ggcgctttcg ggtgtcgctg 180 atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca aggccacgat 240 gcatagccga cctgagaggg tgatcggcca cattgggact gagacacggc ccaaactcct 300 acgggaggca gcagtaggga atcttcggca atggacgaaa gtctgaccga gcaacgccgc 360 gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac aaggatgaga 420 gtaactgttc atcccttgac ggtatctaac cagaaagcca cggctaacta cgtgccagca 480 gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca 540 ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac 600 tgggagactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg aaatgcgtag 660 atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggct 720 cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgag 780 tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta agcactccgc 840 ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc gcacaagcgg 900 tggagcatgt ggtttaattc gaagcaacgc gaagaacctt accaggtctt gacatccttt 960 gaccactcta gagatagagc ttcttcgggg gcaaagtgac aggtggtgca tggttgtcgt 1020 cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct tattgttagt 1080 tgccatcatt cagttgggca ctctagcaag actgccggtg acaaaccgga ggaaggtggg 1140 gatgacgtca aatcatcatg ccccttatga cctgggctac acacgtgcta caatgggaag 1200 tacaacgagt tgcgaagtcg cgaggctaag ctaatctctt aaagcttctc tcagttcgga 1260 ttgtaggctg caactcgcct acatgaagcc ggaatcgcta gtaatcgcgg atcagcacgc 1320 cgcggtgaat acgttcccgg gccttgtaca caccgcccgt cacaccacga gagtttgtaa 1380 cacccgaagt cggtgaggta accttttgga gccagccgcc taaggtggga tagatgattg 1440 gggtgaagc 1449 <210> 154

<211> 1449

<212> DNA

<213> Enterococcus malodoratus 4

<220>

<221 > misc_feature

<222> (1244).. (1244)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (1254).. (1254)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (1412).. (1412)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (1426).. (1426)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (1434).. (1434)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (1440).. (1440)

<223> n is a, c, g, or t

<400> 154 gggcagcata tatgcagtca acgctttttc tttcaccgga gcttgctcca ccgaaagaaa 60 aagagtggcg aacgggtgag taacacgtgg gtaacctgcc catcagaagg ggataacact 120 tggaaacagg tgctaatacc gtataacagt agaaaccgca tggtttctat ttgaaaggcg 180 cttttgcgtc actgatggat ggacccgcgg tgcattagct agttggtgag gtaacggctc 240 accaaggcaa cgatgcatag ccgacctgag agggtgatcg gccacattgg gactgagaca 300 cggcccaaac tcctacggga ggcagcagta gggaatcttc ggcaatggac gaaagtctga 360 ccgagcaacg ccgcgtgagt gaagaaggtt ttcggatcgt aaaactctgt tgttagagaa 420 gaacaaggat gagagtaaaa cgttcatccc ttgacggtat ctaaccagaa agccacggct 480 aactacgtgc cagcagccgc ggtaatacgt aggtggcaag cgttgtccgg atttattggg 540 cgtaaagcga gcgcaggcgg tttcttaagt ctgatgtgaa agcccccggc tcaaccgggg 600 agggtcattg gaaactggga aacttgagtg cagaagagga gagtggaatt ccatgtgtag 660 cggtgaaatg cgtagatata tggaggaaca ccagtggcga aggcggctct ctggtctgta 720 actgacgctg aggctcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac 780 gccgtaaacg atgagtgcta agtgttggag ggtttccgcc cttcagtgct gcagctaacg 840 cattaagcac tccgcctggg gagtacgacc gcaaggttga aactcaaagg aattgacggg 900 ggcccgcaca agcggtggag catgtggttt aattcgaagc aacgcgaaga accttaccag 960 gtcttgacat cctttgacca ctctagagat agagcttccc cttcgggggc aaagtgacag 1020 gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc 1080 gcaaccctta ttgttagttg ccatcattta gttgggcact ctagcgagac tgccggtgac 1140 aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac 1200 acgtgctaca atgggaagta caacgagtcg cgaagtcgcg aggntaagct aatntcttaa 1260 agcttctctc agttcggatt gtaggctgca actcgcctac atgaagccgg aatcgctagt 1320 aatcgcggat cagcacgccg cggtgaatac gttcccgggc cttgtacaca ccgcccgtca 1380 caccacgaga gtttgtaaca cccgaagtcg tngaggtaac ctttgnagcc agcngcttan 1440 gtgtttatt 1449

<210> 155

<211> 1447

<212> DNA

<213> Enterococcus faecium

<400> 155 ttgggcgttt cgatgccagt tatcgttcca cacggaaaaa gaggccctcg tgggaacggg 60 tgagtaacac gtgggtaacc tgcccatcag aaggggataa cacttggaaa caggtgctaa 120 taccgtataa caatcgaaac cgcatggttt tgatttgaaa ggcgctttcg ggtgtcgctg 180 atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca aggccacgat 240 gcatagccga cctgagaggg tgatcggcca cattgggact gagacacggc ccaaactcct 300 acgggaggca gcagtaggga atcttcggca atggacgaaa gtctgaccga gcaacgccgc 360 gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac aaggatgaga 420 gtaactgttc atcccttgac ggtatctaac cagaaagcca cggctaacta cgtgccagca 480 gccgcggtaa tacgtaggtg gcaagcgttg tccgggattt attgggcgta aagcgagcgc 540 aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa cctggggagg gtcattggaa 600 actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg tgaaatgcgt 660 agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact gacgctgagg 720 ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc gtaaacgatg 780 agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat taagcactcc 840 gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc 900 ggtggagcat gtgggtttaa ttcgaagcaa cgcgaagaac cttaccaggt cttgacatcc 960 tttgaccacc tctagagata gagcttcccc ttcgggggca aagtgacagg tggtgcatgg 1020 ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccttat 1080 tgttagttgc catcattcag ttgggcactc tagcaagact gccggtgaca aaccggagga 1140 aggtggggat gacgtcaaat catcatgccc cttatgacct gggctacaca cgtgctacaa 1200 tgggaagtac aacgagttgc gaagtcgcga ggctaagcta atctcttaaa gcttctctca 1260 gttcggattg caggctgcaa ctcgcctgca tgaagccgga atcgctagta atcgcggatc 1320 agcacgccgc ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accacgagag 1380 tttgtaacac ccgaagtcgg ttgaggtaac ctttggagcc agcccggcct taaaaggggg 1440 atatagt 1447

<210> 156

<211> 1441

<212> DNA

<213> Enterococcus raffinosus 6

<220>

<221 > misc_feature

<222> (17).. (17)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (28).. (28)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (1409).. (1409)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (1418).. (1418)

<223> n is a, c, g, or t

<220>

<221 > misc_feature

<222> (1438)..(1438)

<223> n is a, c, g, or t

<400> 156 ggctatcatg cagtcgnacg ctttttcnat caccggagct tgctccaccg aaagaaaagg 60 agtggcgaac gggtgagtaa cacgtgggta acctgcccat cagaagggga taacacttgg 120 aaacaggtgc taataccgta taacaataga aaccgcatgg tttctatttg aaaggcgctt 180 ttgcgccact gatggatgga cccgcggtgc attagctagt tggtgaggta acggctcacc 240 aaggcaacga tgcatagccg acctgagagg gtgatcggcc acattgggac tgagacacgg 300 cccaaactcc tacgggaggc agcagtaggg aatcttcggc aatggacgaa agtctgaccg 360 agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt tagagaagaa 420 caaggatgag agtaaaatgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggaaac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 900 ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960 ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa gtgacaggtg 1020 gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca 1080 acccttattg ttagttgcca tcatttagtt gggcactcta gcgagactgc cggtgacaaa 1140 ccggaggaag gtggggatga cgtcaaatca tcatgcccct tatgacctgg gctacacacg 1200 tgctacaatg ggaagtacaa cgagtcgcga agtcgcgagg ctaagctaat ctcttaaagc 1260 ttctctcagt tcggattgta ggctgcaact cgcctacatg aagccggaat cgctagtaat 1320 cgcggatcag cacgccgcgg tgaatacgtt cccgggcctt gtacacaccg cccgtcacac 1380 cacgagagtt tgtaacaccc gaagtcgtng aggtaacnct ttggagccag ccgcctangg 1440 g 1441

<210> 157

<211> 1437

<212> DNA

<213> Enterococcus sp. 115w

<400> 157 gctataatgc agtcgtacgc tttttctttc accggagctt gctccaccga aagaaaagga 60 gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat aacacttgga 120 aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga aaggcgcttt 180 cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac 240 caaggccacg atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg 300 gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc 360 gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga 420 acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc cacggctaac 480 tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt tattgggcgt 540 aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca accggggagg 600 gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca tgtgtagcgg 660 tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cgactctctg gtctgtaact 720 gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgcc 780 gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca gctaacgcat 840 taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc 900 ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc 960 ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa gtgacaggtg 1020 gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca 1080 acccttattg ttagttgcca tcatttagtt gggcattcta gcaagactgc cggtgacaaa 1140 ccggaggaag gtggggagga ggtcaaatca tcatgcccct tatgacctgg gctacacacg 1200 tgttccaatg ggaagtacaa ggagtcggga agtcgggagg ttaagttaat ttcttaaagc 1260 ttttttcagt tcggattgta ggctgcaatt cgcctacatg aagccggaat cgttagtaat 1320 cgcggatcac cacgccgcgg ggaatacgtt cccggccctt gtacacaccg cccgtcacac 1380 cacgaaagtt tgtaacaccg gaagtcggtg aggtaacctt ttggagccag ccgccta 1437

<210> 158

<211> 1435

<212> DNA

<213> Enterococcus durans 2

<400> 158 cgcttctttt tcatcggagc ttgctcaycg ggaaaagaag agtggcgaac gggtgagtaa 60 cacgtgggta actgcccatc agaaggggat aacacttgga aacaggtgct aataccgtat 120 aacaatcgaa accgcatggt tttgatttga aaggcgcttt cgggtgtcgc tgatggatgg 180 acccgcggtg cattagctag ttggtgaggt aacggctcac caaggccacg atgcatagcc 240 gacctgagag ggtgatcggc cacattggga ctgagacacg gcccaaactc ctacgggagg 300 cagcagtagg gaatcttcgg caatggacga aagtctgacc gagcaacgcc gcgtgagtga 360 agaaggtttt cggatcgtaa aactctgttg ttagagaaga acaaggatga gagtaactgt 420 tcatcccttg acggtatcta accagaaagc cacggctaac tacgtgccag cagccgcggt 480 aatacgtagg tggcaagcgt tgtccggatt tattgggcgt aaagcgagcg caggcggttt 540 cttaagtctg atgtgaaagc ccccggctca accggggagg gtcattggaa actgggagac 600 ttgagtgcag aagaggagag tggaattcca tgtgtagcgg tgaaatgcgt agatatatgg 660 aggaacacca gtggcgaagg cggctctctg gtctgtaact gacgctgagg ctcgaaagcg 720 tggggagcaa acaggattag ataccctggt agtccacgcc gtaaacgatg agtgctaagt 780 gttggagggt ttccgccctt cagtgctgca gctaacgcat taagcactcc gcctggggag 840 tacgaccgca aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat 900 gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc ttgacatcct ttgaccactc 960 tagagataga gcttcccttc gggggcaaag tgacaggtgg tgcatggttg tcgtcagctc 1020 gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa cccttattgt tagttgccat 1080 cattcagttg ggcactctag caagactgcc ggtgacaaac cggaggaagg tggggatgac 1140 gtcaaatcat catgcccctt atgacctggg ctacacacgt gctacaatgg gaagtacaac 1200 gagttgcgaa gtcgcgaggc taagctaatc tcttaaagct tctctcagtt cggattgtag 1260 gctgcaactc gcctacatga agccggaatc gctagtaatc gcggatcagc acgccgcggt 1320 gaatacgttc ccgggccttg tacacaccgc ccgtcacacc acgagagttt gtaacacccg 1380 aagtcggtga ggtaaccttt tggagccagc cgcctaaggt gggatagatg attgg 1435

<210> 159

<211> 1435

<212> DNA

<213> Enterococcus xiangfangensis

<400> 159 cgctttttct ttcaccggag cttgctccac cgaaagaaaa agagtggcga acgggtgagt 60 aacacgtggg taacctgccc atcagaaggg gataacactt ggaaacaggt gctaataccg 120 tataacaagt aaaaccgcat ggttttactt tgaaaggcgc ttttgcgtca ctgatggatg 180 gacccgcggt gcattagcta gttggtgagg taacggctca ccaaggcaac gatgcatagc 240 cgacctgaga gggtgatcgg ccacattggg actgagacac ggcccaaact cctacgggag 300 gcagcagtag ggaatcttcg gcaatggacg aaagtctgac cgagcaacgc cgcgtgagtg 360 aagaaggttt tcggatcgta aaactctgtt gttagagaag aacaaggatg agagtaaaaa 420 gttcatccct tgacggtatc taaccagaaa gccacggcta actacgtgcc agcagccgcg 480 gtaatacgta ggtggcaagc gttgtccgga tttattgggc gtaaagcgag cgcaggcggt 540 ttcttaagtc tgatgtgaaa gcccccggct caaccgggga gggtcattgg aaactgggaa 600 acttgagtgc agaagaggag agtggaattc catgtgtagc ggtgaaatgc gtagatatat 660 ggaggaacac cagtggcgaa ggcggctctc tggtctgtaa ctgacgctga ggctcgaaag 720 cgtggggagc aaacaggatt agataccctg gtagtccacg ccgtaaacga tgagtgctaa 780 gtgttggagg gtttccgccc ttcagtgctg cagctaacgc attaagcact ccgcctgggg 840 agtacgaccg caaggttgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc 900 atgtggttta attcgaagca acgcgaagaa ccttaccagg tcttgacatc ctttgaccac 960 tctagagata gagcttcccc ttcgggggca aagtgacagg tggtgcatgg ttgtcgtcag 1020 ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccttat tgttagttgc 1080 catcatttag ttgggcactc tagcgagact gccggtgaca aaccggagga aggtggggat 1140 gacgtcaaat catcatgccc cttatgacct gggctacaca cgtgctacaa tgggaagtac 1200 aacgagtcgc gaagtcgcga ggctaagcta atctcttaaa gcttctctca gttcggattg 1260 taggctgcaa ctcgcctaca tgaagccgga atcgctagta atcgcggatc agcacgccgc 1320 ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac 1380 ccgaagtcgg tgaggtaacc tttttggagc cagccgccta aggtgggata gatga 1435

<210> 160

<211> 1431

<212> DNA

<213> bacterium MRG-IFC-2

<400> 160 aaatatgcag tcgtacgctt ctttttaccg gagcttgctc caccggaaaa agggagtggc 60 gaacgggtga gtaacacgtg ggtaacctgc ccatcagaag gggataacac ttggaaacag 120 gtgctaatac cgtataacaa tcgaaaccgc atggttttga tttgaaaggc gctttcgggt 180 gtcgctgatg gatggacccg cggtgcatta gctagttggt gaggtaacgg ctcaccaagg 240 ccacgatgca tagccgacct gagagggtga tcggccacat tgggactgag acacggccca 300 aactcctacg ggaggcagca gtagggaatc ttcggcaatg gacgaaagtc tgaccgagca 360 acgccgcgtg agtgaagaag gttttcggat cgtaaaactc tgttgttaga gaagaacaag 420 gatgagagta actgttcatc ccttgacggt atctaaccag aaagccacgg ctaactacgt 480 gccagcagcc gcggtaatac gtaggtggca agcgttgtcc ggatttattg ggcgtaaagc 540 gagcgcaggc ggtttcttaa gtctgatgtg aaagcccccg gctcaaccgg ggagggtcat 600 tggaaactgg gagacttgag tgcagaagag gagagtggaa ttccatgtgt agcggtgaaa 660 tgcgtagata tatggaggaa caccagtggc gaaggcggct ctctggtctg taactgacgc 720 tgaggctcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa 780 cgatgagtgc taagtgttgg agggtttccg cccttcagtg ctgcagctaa cgcattaagc 840 actccgcctg gggagtacga ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca 900 caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac 960 atcctttgac cactctagag atagagcttc cccttcgggg gcaaagtgac aggtggtgca 1020 tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct 1080 tattgttagt tgccatcatt cagttgggca ctctagcaag actgccggtg acaaaccgga 1140 ggaaggtggg gatgacgtca aatcatcatg ccccttatga cctgggctac acacgtgcta 1200 caatgggaag tacaacgagt tgcgaagtcg cgaggctaag ctaatctctt aaagcttctc 1260 tcagttcgga ttgcaggctg caactcgcct gcatgaagcc ggaatcgcta gtaatcgcgg 1320 atcagcacgc cgcggtgaat acgttcccgg gccttgtaca caccgcccgt cacaccacga 1380 gagtttgtaa cacccgaagt cggtgaggta accttttgga gccagccgca t 1431

<210> 161

<211> 1427

<212> DNA

<213> Enterococcus sp. KS40

<400> 161 tgcagtcgac gctttttctt tcaccggagc ttgctccacc gaaagaaaag gagtggcgaa 60 cgggtgagta acacgtgggt aacctgccca tcagaagggg ataacacttg gaaacaggtg 120 ctaataccgt ataataagta aaaccgcatg gttttacttt gaaaggcgct tttgcgtcac 180 tgatggatgg acccgcggtg cattagctag ttggtgaggt aacggctcac caaggcaacg 240 atgcatagcc gacctgagag ggtgatcggc cacattggga ctgagacacg gcccaaactc 300 ctacgggagg cagcagtagg gaatcttcgg caatggacga aagtctgacc gagcaacgcc 360 gcgtgagtga agaaggtttt cggatcgtaa aactctgttg ttagagaaga acaaggatga 420 gagtaaaacg ttcatccctt gacggtatct aaccagaaag ccacggctaa ctacgtgcca 480 gcagccgcgg taatacgtag gtggcaagcg ttgtccggat ttattgggcg taaagcgagc 540 gcaggcggtt tcttaagtct gatgtgaaag cccccggctc aaccggggag ggtcattgga 600 aactgggaaa cttgagtgca gaagaggaga gtggaattcc atgtgtagcg gtgaaatgcg 660 tagatatatg gaggaacacc agtggcgaag gcggctctct ggtctgtaac tgacgctgag 720 gctcgaaagc gtggggagca aacaggatta gataccctgg tagtccacgc cgtaaacgat 780 gagtgctaag tgttggaggg tttccgccct tcagtgctgc agctaacgca ttaagcactc 840 cgcctgggga gtacgaccgc aaggttgaaa ctcaaaggaa ttgacggggg cccgcacaag 900 cggtggagca tgtggtttaa ttcgaagcaa cgcgaagaac cttaccaggt cttgacatcc 960 tttgaccact ctagagatag agcttcccct tcgggggcaa agtgacaggt ggtgcatggt 1020 tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccttatt 1080 gttagttgcc atcatttagt tgggcactct agcgagactg ccggtgacaa accggaggaa 1140 ggtggggatg acgtcaaatc atcatgcccc ttatgacctg ggctacacac gtgctacaat 1200 gggaagtaca acgagtcgcg aagtcgcgag gctaagctaa tctcttaaag cttctctcag 1260 ttcggattgt aggctgcaac tcgcctacat gaagccggaa tcgctagtaa tcgcggatca 1320 gcacgccgcg gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccacgagagt 1380 ttgtaacacc cgaagtcggt gaggtaacct tttggagcca gccgcct 1427 <210> 162

<211> 1426

<212> DNA

<213> Enterococcus faecalis

<400> 162 agtcgtacgc ttctttttca ccggagcttg ctccaccgga aaaagaagag tggcgaacgg 60 gtgagtaaca cgtgggtaac ctgcccatca gaaggggata acacttggaa acaggtgcta 120 ataccgtata acaatcgaaa ccgcatggtt ttgatttgaa aggcgctttc gggtgtcgct 180 gatggatgga cccgcggtgc attagctagt tggtgaggta acggctcacc aaggccacga 240 tgcatagccg acctgagagg gtgatcggcc acattgggac tgagacacgg cccaaactcc 300 tacgggaggc agcagtaggg aatcttcggc aatggacgaa agtctgaccg agcaacgccg 360 cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt tagagaagaa caaggatgag 420 agtaactgtt catcccttga cggtatctaa ccagaaagcc acggctaact acgtgccagc 480 agccgcggta atacgtaggt ggcaagcgtt gtccggattt attgggcgta aagcgagcgc 540 aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg tcattggaaa 600 ctgggagact tgagtgcaga agaggagagt ggaattccat gtgtagcggt gaaatgcgta 660 gatatatgga ggaacaccag tggcgaaggc ggctctctgg tctgtaactg acgctgaggc 720 tcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccg taaacgatga 780 gtgctaagtg ttggagggtt tccgcccttc agtgctgcag ctaacgcatt aagcactccg 840 cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc cgcacaagcg 900 gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaggtct tgacatcctt 960 tgaccactct agagatagag cttccccttc gggggcaaag tgacaggtgg tgcatggttg 1020 tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa cccttattgt 1080 tagttgccat cattcagttg ggcactctag caagactgcc ggtgacaaac cggaggaagg 1140 tggggatgac gtcaaatcat catgcccctt atgacctggg ctacacacgt gctacaatgg 1200 gaagtacaac gagttgcgaa gtcgcgaggc taagctaatc tcttaaagct tctctcagtt 1260 cggattgcag gctgcaactc gcctgcatga agccggaatc gctagtaatc gcggatcagc 1320 acgccgcggt gaatacgttc ccgggccttg tacacaccgc ccgtcacacc acgagagttt 1380 gtaacacccg aagtcggtga ggtaaccttt ttggagccag ccgcct 1426

<210> 163

<211> 1425

<212> DNA

<213> Enterococcus viikkiensis 5

<400> 163 accggagctt gctcaccgaa agaaaaagag tggcgaacgg gtgagtaaca cgtgggtaac 60 ctgcccatca gaaggggata acacttggaa acaggtgcta ataccgtata acaagtaaaa 120 ccgcatggtt ttactttgaa aggcgctttt gcgtcactga tggatggacc cgcggtgcat 180 tagctagttg gtgaggtaac ggctcaccaa ggcaacgatg catagccgac ctgagagggt 240 gatcggccac attgggactg agacacggcc caaactccta cgggaggcag cagtagggaa 300 tcttcggcaa tggacgaaag tctgaccgag caacgccgcg tgagtgaaga aggttttcgg 360 atcgtaaaac tctgttgtta gagaagaaca aggatgagag taaaatgttc atcccttgac 420 ggtatctaac cagaaagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg 480 gcaagcgttg tccggattta ttgggcgtaa agcgagcgca ggcggtttct taagtctgat 540 gtgaaagccc ccggctcaac cggggagggt cattggaaac tgggaaactt gagtgcagaa 600 gaggagagtg gaattccatg tgtagcggtg aaatgcgtag atatatggag gaacaccagt 660 ggcgaaggcg gctctctggt ctgtaactga cgctgaggct cgaaagcgtg gggagcaaac 720 aggattagat accctggtag tccacgccgt aaacgatgag tgctaagtgt tggagggttt 780 ccgcccttca gtgctgcagc taacgcatta agcactccgc ctggggagta cgaccgcaag 840 gttgaaactc aaaggaattg acgggggccc gcacaagcgg tggagcatgt ggtttaattc 900 gaagcaacgc gaagaacctt accaggtctt gacatccttt gaccactcta gagatagagc 960 ttcttcgggg gcaaagtgac aggtggtgca tggttgtcgt cagctcgtgt cgtgagatgt 1020 tgggttaagt cccgcaacga gcgcaaccct tattgttagt tgccatcatt tagttgggca 1080 ctctagcgag actgccggtg acaaaccgga ggaaggtggg gatgacgtca aatcatcatg 1140 ccccttatga cctgggctac acacgtgcta caatgggaag tacaacgagt cgcgaagtcg 1200 cgaggctaag ctaatctctt aaagcttctc tcagttcgga ttgtaggctg caactcgcct 1260 acatgaagcc ggaatcgcta gtaatcgcgg atcagcacgc cgcggtgaat acgttcccgg 1320 gccttgtaca caccgcccgt cacaccacga gagtttgtaa cacccgaagt cggtgaggta 1380 accttttgga gccagccgcc taaggtggga tagatgattg gggtg 1425

<210> 164

<211> 1424

<212> DNA

<213> Enterococcus pseudoavium 3

<400> 164 tggcggcgtg cctaatacat gcaagtcgaa cgctttttct ttcaccggag cttgctccac 60 cgaaagaaaa agagtggcga acgggtgagt aacacgtggg taacctgccc atcagaaggg 120 gataacactt ggaaacaggt gctaataccg tataacaagt aaaaccgcat ggttttactt 180 tgaaaggcgc ttttgcgtca ctgatggatg gacccgcggt gcattagcta gttggtgagg 240 taacggctca ccaaggcaac gatgcatagc cgacctgaga gggtgatcgg ccacattggg 300 actgagacac ggcccaaact cctacgggag gcagcagtag ggaatcttcg gcaatggacg 360 aaagtctgac cgagcaacgc cgcgtgagtg aagaaggttt tcggatcgta aaactctgtt 420 gttagagaag aacaaggatg agagtaaaat gttcatccct tgacggtatc taaccagaaa 480 gccacggcta actacgtgcc agcagccgcg gtaatacgta ggtggcaagc gttgtccgga 540 tttattgggc gtaaagcgag cgcaggcggt ttcttaagtc tgatgtgaaa gcccccggct 600 caaccgggga gggtcattgg aaactgggaa acttgagtgc agaagaggag agtggaattc 660 catgtgtagc ggtgaaatgc gtagatatat ggaggaacac cagtggcgaa ggcggctctc 720 tggtctgtaa ctgacgctga ggctcgaaag cgtggggagc aaacaggatt agataccctg 780 gtagtccacg ccgtaaacga tgagtgctaa gtgttggagg gtttccgccc ttcagtgctg 840 cagctaacgc attaagcact ccgcctgggg agtacgaccg caaggttgaa actcaaagga 900 attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgaagaa 960 ccttaccagg tcttgacatc ctttgaccac tctagagata gagcttcccc ttcgggggca 1020 aagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc 1080 gcaacgagcg caacccttat tgttagttgc catcatttag ttgggcactc tagcgagact 1140 gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct 1200 gggctacaca cgtgctacaa tgggaagtac aacgagtcgc gaagtcgcga ggctaagcta 1260 atctcttaaa gcttctctca gttcggattg taggctgcaa ctcgcctaca tgaagccgga 1320 atcgctagta atcgcggatc agcacgccgc ggtgaatacg ttcccgggcc ttgtacacac 1380 cgcccgtcac accacgagag tttgtaacac ccgaagtcgg tgag 1424

<210> 165

<211> 1419

<212> DNA

<213> Enterococcus sp. SB

<400> 165 tgcagtcgac gcctcttttt ccaccggagc ttgctccatc ggaaaaagag gagtggcgaa 60 cgggtgagta acacgtgggt aacctgccca tcagaagggg ataacacttg gaaacaggtg 120 ctaataccgt ataacaatcg aaaccgcatg gtttcgattt gaaaggcgct ttcgggtgtc 180 gctgatggat ggacccgcgg tgcattagct agttggtgag gtaacggctc accaaggcga 240 cgatgcatag ccgacctgag agggtgatcg gccacattgg gactgagaca cggcccaaac 300 tcctacggga ggcagcagta gggaatcttc ggcaatggac gaaagtctga ccgagcaacg 360 ccgcgtgagt gaagaaggtt ttcggatcgt aaaactctgt tgttagagaa gaacaaggat 420 gagagtaact gttcatccct tgacggtatc taaccagaaa gccacggcta actacgtgcc 480 agcagccgcg gtaatacgta ggtggcaagc gttgtccgga tttattgggc gtaaagcgag 540 cgcaggcggt ttcttaagtc tgatgtgaaa gcccccggct caaccgggga gggtcattgg 600 aaactgggag acttgagtgc agaagaggag agtggaattc catgtgtagc ggtgaaatgc 660 gtagatatat ggaggaacac cagtggcgaa ggcggctctc tggtctgtaa ctgacgctga 720 ggctcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg ccgtaaacga 780 tgagtgctaa gtgttggagg gtttccgccc ttcagtgctg cagctaacgc attaagcact 840 ccgcctgggg agtacgaccg caaggttgaa actcaaagga attgacgggg gcccgcacaa 900 gcggtggagc atgtggttta attcgaagca acgcgaagaa ccttaccagg tcttgacatc 960 ctttgaccac tctagagata gagcttcccc ttcgggggca aagtgacagg tggtgcatgg 1020 ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccttat 1080 tgttagttgc catcatttag ttgggcactc tagcaagact gccggtgaca aaccggagga 1140 aggtggggat gacgtcaaat catcatgccc cttatgacct gggctacaca cgtgctacaa 1200 tgggaagtac aacgagtcgc aaagtcgcga ggctaagcta atctcttaaa gcttctctca 1260 gttcggattg taggctgcaa ctcgcctaca tgaagccgga atcgctagta atcgcggatc 1320 agcacgccgc ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accacgagag 1380 tttgaacacc cgaagtcggt gaggtaacct ttggagcca 1419

<210> 166

<211> 1416

<212> DNA

<213> bacterium mpn-isolate group 13

<400> 166 tctatcccac cttaggcggc tggctccaaa aggttacctc accgacttcg ggtgttacaa 60 actctcgtgg tgtgacgggc ggtgtgtaca aggcccggga acgtattcac cgcggcgtgc 120 tgatccgcga ttactagcga ttccggcttc atgtaggcga gttgcagcct acaatccgaa 180 ctgagagaag ctttaagaga ttagcttagc ctcgcgactt tgcgactcgt tgtacttccc 240 attgtagcac gtgtgtagcc caggtcataa ggggcatgat gatttgacgt catccccacc 300 ttcctccggt ttgtcaccgg cagtcttgct agagtgccca actaaatgat ggcaactaac 360 aataagggtt gcgctcgttg cgggacttaa cccaacatct cacgacacga gctgacgaca 420 accatgcacc acctgtcact ttgcccccga aggggaagct ctatctctag agtggtcaaa 480 ggatgtcaag acctggtaag gttcttcgcg ttgcttcgaa ttaaaccaca tgctccaccg 540 cttgtgcggg cccccgtcaa ttcctttgag tttcaacctt gcggtcgtac tccccaggcg 600 gagtgcttaa tgcgttagct gcagcactga agggcggaaa ccctccaaca cttagcactc 660 atcgtttacg gcgtggacta ccagggtatc taatcctgtt tgctccccac gctttcgagc 720 ctcagcgtca gttacagacc agagagccgc cttcgccact ggtgttcctc catatatcta 780 cgcatttcac cgctacacat ggaattccac tctcctcttc tgcactcaag tctcccagtt 840 tccaatgacc ctccccggtt gagccggggg ctttcacatc agacttaaga aaccgcctgc 900 gctcgcttta cgcccaataa atccggacaa cgcttgccac ctacgtatta ccgcggctgc 960 tggcacgtag ttagccgtgg ctttctggtt agataccgtc aagggatgaa cagttactct 1020 catccttgtt cttctctaac aacagagttt tacgatccga aaaccttctt cactcacgcg 1080 gcgttgctcg gtcagacttt cgtccattgc cgaagattcc ctactgctgc ctcccgtagg 1140 agtttgggcc gtgtctcagt cccaatgtgg ccgatcaccc tctcaggtcg gctatgcatc 1200 gtcgccttgg tgagccgtta cctcaccaac tagctaatgc accgcgggtc catccatcag 1260 cgacacccga aagcgccttt caaatcaaaa ccatgcggtt tcgattgtta tacggtatta 1320 gcacctgttt ccaagtgtta tccccttctg atgggcaggt tacccacgtg ttactcaccc 1380 gttcgccact cctctttttc cggtggagca agctct 1416

<210> 167

<211> 1408

<212> DNA

<213> Enterococcus avium 4

<400> 167 ctgcccatca gaaggggata acacttggaa acaggtgcta ataccgtata acagtagaaa 60 ccgcatggtt tctatttgaa aggcgctttt gcgtcactga tggatggacc cgcggtgcat 120 tagctagttg gtgaggtaac ggctcaccaa ggcaacgatg catagccgac ctgagagggt 180 gatcggccac attgggactg agacacggcc caaactccta cgggaggcag cagtagggaa 240 tcttcggcaa tggacgaaag tctgaccgag caacgccgcg tgagtgaaga aggttttcgg 300 atcgtaaaac tctgttgtta gagaagaaca aggatgagag taaaacgttc atcccttgac 360 ggtatctaac cagaaagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg 420 gcaagcgttg tccggattta ttgggcgtaa agcgagcgca ggcggtttct taagtctgat 480 gtgaaagccc ccggctcaac cggggagggt cattggaaac tgggaaactt gagtgcagaa 540 gaggagagtg gaattccatg tgtagcggtg aaatgcgtag atatatggag gaacaccagt 600 ggcgaaggcg gctctctggt ctgtaactga cgctgaggct cgaaagcgtg ggagcaaaca 660 ggattagata ccctggtagt ccacgccgta aacgatgagt gctaagtgtt ggagggtttc 720 cgcccttcag tgctgcagct aacgcattaa gcactccgcc tggggagtac gaccgcaagg 780 ttgaaactca aaggaattga cgggggcccg cacaagcggt ggagcatgtg gtttaattcg 840 aagcaacgcg aagaacctta ccaggtcttg acatcctttg accactctag agatagagct 900 tccccttcgg gggcaaagtg acaggtggtg catggttgtc gtcagctcgt gtcgtgagat 960 gttgggttaa gtcccgcaac gagcgcaacc cttattgtta gttgccatca tttagttggg 1020 cactctagcg agactgccgg tgacaaaccg gaggaaggtg gggatgacgt caaatcatca 1080 tgccccttat gacctgggct acacacgtgc tacaatgggg agtacaacga gtcgcgaagt 1140 cgcgaggcta agctaatctc ttaaagcttc tctcagttcg gattgtaggc tgcaactcgc 1200 ctacatgaag ccggaatcgc tagtaatcgc ggatcagcac gccgcggtga atacgttccc 1260 gggccttgta cacaccgccc gtcacaccac gagagtttgt aacacccgaa gtcggtgagg 1320 taaccttttg gagccagccg cctaaggtgg gatagatgat tggggtgaag tcgtaacaag 1380 gtagccgtat cggaaggtgc ggctggat 1408

<210> 168

<211> 1401

<212> DNA

<213> Enterococcus faecium 2

<400> 168 gcttcttttt ccaccggagc ttgctccacc ggaaaaagaa gagtggcgaa cgggtgagta 60 acacgtgggt aacctgccca tcagaagggg ataacacttg gaaacaggtg ctaataccgt 120 ataacaatcg aaaccgcatg gttttgattt gaaaggcgct ttcgggtgtc gctgatggat 180 ggacccgcgg tgcattagct agttggtgag gtaacggctc accaaggcca cgatgcatag 240 ccgacctgag agggtgatcg gccacattgg gactgagaca cggcccaaac tcctacggga 300 ggcagcagta gggaatcttc ggcaatggac gaaagtctga ccgagcaacg ccgcgtgagt 360 gaagaaggtt ttcggatcgt aaaactctgt tgttagagaa gaacaaggat gagagtaact 420 gttcatccct tgacggtatc taaccagaaa gccacggcta actacgtgcc agcagccgcg 480 gtaatacgta ggtggcaagc gttgtccgga tttattgggc gtaaagcgag cgcaggcggt 540 ttcttaagtc tgatgtgaaa gcccccggct caaccgggga gggtcattgg aaactgggag 600 acttgagtgc agaagaggag agtggaattc catgtgtagc ggtgaaatgc gtagatatat 660 ggaggaacac cagtggcgaa ggcggctctc tggtctgtaa ctgacgctga ggctcgaaag 720 cgtggggagc aaacaggatt agataccctg gtagtccacg ccgtaaacga tgagtgctaa 780 gtgttggagg gtttccgccc ttcagtgctg cagctaacgc attaagcact ccgcctgggg 840 agtacgaccg caaggttgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc 900 atgtggttta attcgaagca acgcgaagaa ccttaccagg tcttgacatc ctttgaccac 960 tctagagata gagcttcccc ttcgggggca aagtgacagg tggtgcatgg ttgtcgtcag 1020 ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccttat tgttagttgc 1080 catcattcag ttgggcactc tagcaagact gccggtgaca aaccggagga aggtggggat 1140 gacgtcaaat catcatgccc cttatgacct gggctacaca cgtgctacaa tgggaagtac 1200 aacgagttgc gaagtcgcga ggctaagcta atctcttaaa gcttctctca gttcggattg 1260 caggctgcaa ctcgcctgca tgaagccgga atcgctagta atcgcggatc agcacgccgc 1320 ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac 1380 ccgaagtcgg tgaggtaacc t 1401

<210> 169

<211> 1397

<212> DNA

<213> Enterococcus faecium 3

<400> 169 gcttcttttt ccaccggagc ttgctccacc ggaaaaagaa gagtggcgaa cgggtgagta 60 acacgtgggt aacctgccca tcagaagggg ataacacttg gaaacaggtg ctaataccgt 120 ataacaatcg aaaccgcatg gttttgattt gaaaggcgct ttcgggtgtc gctgatggat 180 ggacccgcgg tgcattagct agttggtgag gtaacggctc accaaggcca cgatgcatag 240 ccgacctgag agggtgatcg gccacattgg gactgagaca cggcccaaac tcctacggga 300 ggcagcagta gggaatcttc ggcaatggac gaaagtctga ccgagcaacg ccgcgtgagt 360 gaagaaggtt ttcggatcgt aaaactctgt tgttagagaa gaacaaggat gagagtaact 420 gttcatccct tgacggtatc taaccagaaa gccacggcta actacgtgcc agcagccgcg 480 gtaatacgta ggtggcaagc gttgtccgga tttattgggc gtaaagcgag cgcaggcggt 540 ttcttaagtc tgatgtgaaa gcccccggct caaccgggga gggtcattgg aaactgggag 600 acttgagtgc agaagaggag agtggaattc catgtgtagc ggtgaaatgc gtagatatat 660 ggaggaacac cagtggcgaa ggcggctctc tggtctgtaa ctgacgctga ggctcgaaag 720 cgtggggagc aaacaggatt agataccctg gtagtccacg ccgtaaacga tgagtgctaa 780 gtgttggagg gtttccgccc ttcagtgctg cagctaacgc attaagcact ccgcctgggg 840 agtacgaccg caaggttgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc 900 atgtggttta attcgaagca acgcgaagaa ccttaccagg tcttgacatc ctttgaccac 960 tctagagata gagcttcccc ttcgggggca aagtgacagg tggtgcatgg ttgtcgtcag 1020 ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg caacccttat tgttagttgc 1080 catcattcag ttgggcactc tagcaagact gccggtgaca aaccggagga aggtggggat 1140 gacgtcaaat catcatgccc cttatgacct gggctacaca cgtgctacaa tgggaagtac 1200 aacgagttgc gaagtcgcga ggctaagcta atctcttaaa gcttctctca gttcggattg 1260 caggctgcaa ctcgcctgca tgaagccgga atcgctagta atcgcggatc agcacgccgc 1320 ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accacgagag tttgtaacac 1380 ccgaagtcgg tgaggta 1397 <210> 170

<211> 1397

<212> DNA

<213> Enterococcus faecium 4

<400> 170 ttctttttca ccggagcttg ctccaccgga aaaagaagag tggcgaacgg gtgagtaaca 60 cgtgggtaac ctgcccatca gaaggggata acacttggaa acaggtgcta ataccgtata 120 acaatcgaaa ccgcatggtt ttgatttgaa aggcgctttc gggtgtcgct gatggatgga 180 cccgcggtgc attagctagt tggtgaggta acggctcacc aaggccacga tgcatagccg 240 acctgagagg gtgatcggcc acattgggac tgagacacgg cccaaactcc tacgggaggc 300 agcagtaggg aatcttcggc aatggacgaa agtctgaccg agcaacgccg cgtgagtgaa 360 gaaggttttc ggatcgtaaa actctgttgt tagagaagaa caaggatgag agtaactgtt 420 catcccttga cggtatctaa ccagaaagcc acggctaact acgtgccagc agccgcggta 480 atacgtaggt ggcaagcgtt gtccggattt attgggcgta aagcgagcgc aggcggtttc 540 ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg tcattggaaa ctgggagact 600 tgagtgcaga agaggagagt ggaattccat gtgtagcggt gaaatgcgta gatatatgga 660 ggaacaccag tggcgaaggc ggctctctgg tctgtaactg acgctgaggc tcgaaagcgt 720 ggggagcaaa caggattaga taccctggta gtccacgccg taaacgatga gtgctaagtg 780 ttggagggtt tccgcccttc agtgctgcag ctaacgcatt aagcactccg cctggggagt 840 acgaccgcaa ggttgaaact caaaggaatt gacgggggcc cgcacaagcg gtggagcatg 900 tggtttaatt cgaagcaacg cgaagaacct taccaggtct tgacatcctt tgaccactct 960 agagatagag cttccccttc gggggcaaag tgacaggtgg tgcatggttg tcgtcagctc 1020 gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa cccttattgt tagttgccat 1080 cattcagttg ggcactctag caagactgcc ggtgacaaac cggaggaagg tggggatgac 1140 gtcaaatcat catgcccctt atgacctggg ctacacacgt gctacaatgg gaagtacaac 1200 gagttgcgaa gtcgcgaggc taagctaatc tcttaaagct tctctcagtt cggattgcag 1260 gctgcaactc gcctgcatga agccggaatc gctagtaatc gcggatcagc acgccgcggt 1320 gaatacgttc ccgggccttg tacacaccgc ccgtcacacc acgagagttt gtaacacccg 1380 aagtcggtga ggtaacc 1397

<210> 171

<211> 1396

<212> DNA

<213> Prior Sequence 25

<220>

<221 > misc_feature <222> (824).. (824)

<223> n is a, c, g, or t

<400> 171 gacgaacgct ggcggcgtgc ctaatacatg caagtcgaac gctttttctt tcaccggagc 60 ttgctccacc gaaagaaaag gagtggcgaa cgggtgagta acacgtgggt aacctgccca 120 tcagaagggg ataacacttg gaaacaggtg ctaataccgt ataacaagta aaaccgcatg 180 gttttacttt gaaaggcgct tttgcgtcac tgatggatgg acccgcggtg cattagctag 240 ttggtgaggt aacggctcac caaggcaacg atgcatagcc gacctgagag ggtgatcggc 300 cacattggga ctgagacacg gcccaaactc ctacgggagg cagcagtagg gaatcttcgg 360 caatggacga aagtctgacc gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa 420 aactctgttg ttagagaaga acaaggatga gagtagaatg ttcatccctt gacggtatct 480 aaccagaaag ccacggctaa ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg 540 ttgtccggat ttattgggcg taaagcgagc gcaggcggtt tcttaagtct gatgtgaaag 600 cccccggctc aaccggggag ggtcattgga aactgggaaa cttgagtgca gaagaggaga 660 gtggaattcc atgtgtagcg gtgaaatgcg tagatatatg gaggaacacc agtggcgaag 720 gcggctctct ggtctgtaac tgacgctgag gctcgaaagc gtggggagca aacaggatta 780 gataccctgg tagtccacgc cgtaaacgat gagtgctaag tgtnggaggg tttccgccct 840 tcagtgctgc agctaacgca ttaagcactc cgcctgggga gtacgaccgc aaggttgaaa 900 ctcaaaggaa ttgacggggg cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa 960 cgcgaagaac cttaccaggt cttgacatcc tttgaccact ctagagatag agtttcccct 1020 tcgggggcaa agtgacaggt ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg 1080 ttaagtcccg caacgagcgc aacccttatt gttagttgcc atcatttagt tgggcactct 1140 agcgagactg ccggtgacaa accggaggaa ggtggggatg acgtcaaatc atcatgcccc 1200 ttatgacctg ggctacacac gtgctacatt gggaagtaca acgagtcgcg aagtcgcgag 1260 gctaagctaa tctcttaaag cttctctcag ttcggattgt aggctgcaac tcgcctacat 1320 gaagccggaa tcgctagtaa tcgcggatca gcacgccgcg gtgaatacgt tcccgggcct 1380 tgtacacacc gcccgt 1396

<210> 172

<211> 1392

<212> DNA

<213> Enterococcus faecium 5

<400> 172 aacgggtgag taacacgtgg gtaacctgcc catcagaagg ggataacact tggaaacagg 60 tgctaatacc gtataacaat cgaaaccgca tggttttgtt tgaaaggcgc tttcgggtgt 120 cgctgatgga tggacccgcg gtgcattagc tagttggtga ggtaacggct caccaaggcc 180 acgatgcata gccgacctga gagggtgatc ggccacattg ggactgagac acggcccaaa 240 ctcctacggg aggcagcagt agggaatctt cggcaatgga cgaaagtctg accgagcaac 300 gccgcgtgag tgaagaaggt tttcggatcg taaaactctg ttgttagaga agaacaagga 360 tgagagtaac tgttcatccc ttgacggtat ctaaccagaa agccacggct aactacgtgc 420 cagcagccgc ggtaatacgt aggtggcaag cgttgtccgg atttattggg cgtaaagcga 480 gcgcaggcgg tttcttaagt ctgatgtgaa agcccccggc tcaaccgggg agggtcattg 540 gaaactggga gacttgagtg cagaagagga gagtggaatt ccatgtgtag cggtgaaatg 600 cgtagatata tggaggaaca ccagtggcga aggcggctct ctggtctgta actgacgctg 660 aggctcgaaa gcgtggggag caaacaggat tagataccct ggtagtccac gccgtaaacg 720 atgagtgcta agtgttggag ggtttccgcc cttcagtgct gcagctaacg cattaagcac 780 tccgcctggg gagtacgacc gcaaggttga aactcaaagg aattgacggg ggcccgcaca 840 agcggtggag catgtggttt aattcgaagc aacgcgaaga accttaccag gtcttgacat 900 cctttgacca ctctagagat agagcttccc cttcgggggc aaagtgacag gtggtgcatg 960 gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccctta 1020 ttgttagttg ccatcattca gttgggcact ctagcaagac tgccggtgac aaaccggagg 1080 aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac acgtgctaca 1140 atgggaagta caacgagttg cgaagtcgcg aggctaagct aatctcttaa agcttctctc 1200 agttcggatt gcaggctgca actcgcctgc atgaagccgg aatcgctagt aatcgcggat 1260 cagcacgccg cggtgaatac gttcccgggc cttgtacaca ccgcccgtca caccacgaga 1320 gtttgtaaca cccgaagtcg gtgaggtaac ctttggagcc agccgcctaa ggtggataga 1380 tgatggggga ag 1392

<210> 173

<211> 1383

<212> DNA

<213> bacterium MRG-2

<400> 173 ccggagcttg ctccaccgga aaaagaggag tggcgaacgg gtgagtaaca cgtgggtaac 60 ctgcccatca gaaggggata acacttggaa acaggtgcta ataccgtata acaatcgaaa 120 ccgcatggtt ttgatttgaa aggcgctttc gggtgtcgct gatggatgga cccgcggtgc 180 attagctagt tggtgaggta acggctcacc aaggccacga tgcatagccg acctgagagg 240 gtgatcggcc acattgggac tgagacacgg cccaaactcc tacgggaggc agcagtaggg 300 aatcttcggc aatggacgaa agtctgaccg agcaacgccg cgtgagtgaa gaaggttttc 360 ggatcgtaaa actctgttgt tagagaagaa caaggatgag agtaactgtt catcccttga 420 cggtatctaa ccagaaagcc acggctaact acgtgccagc agccgcggta atacgtaggt 480 ggcaagcgtt gtccggattt attgggcgta aagcgagcgc aggcggtttc ttaagtctga 540 tgtgaaagcc cccggctcaa ccggggaggg tcattggaaa ctgggagact tgagtgcaga 600 agaggagagt ggaattccat gtgtagcggt gaaatgcgta gatatatgga ggaacaccag 660 tggcgaaggc ggctctctgg tctgtaactg acgctgaggc tcgaaagcgt ggggagcaaa 720 caggattaga taccctggta gtccacgccg taaacgatga gtgctaagtg ttggagggtt 780 tccgcccttc agtgctgcag ctaacgcatt aagcactccg cctggggagt acgaccgcaa 840 ggttgaaact caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt 900 cgaagcaacg cgaagaacct taccaggtct tgacatcctt tgaccactct agagatagag 960 cttccccttc gggggcaaag tgacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag 1020 atgttgggtt aagtcccgca acgagcgcaa cccttattgt tagttgccat cattcagttg 1080 ggcactctag caagactgcc ggtgacaaac cggaggaagg tggggatgac gtcaaatcat 1140 catgcccctt atgacctggg ctacacacgt gctacaatgg gaagtacaac gagttgcgaa 1200 gtcgcgaggc taagctaatc tcttaaagct tctctcagtt cggattgcag gctgcaactc 1260 gcctgcatga agccggaatc gctagtaatc gcggatcagc acgccgcggt gaatacgttc 1320 ccgggccttg tacacaccgc ccgtcacacc acgagagttt gtaacacccg aagtcggtga 1380 ggt 1383

<210> 174

<211> 1381

<212> DNA

<213> Prior Sequence 26

<400> 174 gacgaacgct ggcggcgtgc ctaatacatg caagtcgaac gcttcttttt ccaccggagc 60 ttgctccacc ggaaaaagag gagtggcgaa cgggtgagta acacgtgggt aacctgccca 120 tcagaagggg ataacacttg gaaacaggtg ctaataccgt ataacaatcg aaaccgcatg 180 gttttgattt gaaaggcgct ttcgggtgtc gctgatggat ggacccgcgg tgcattagct 240 agttggtgag gtaacggctc accaaggcga cgatgcatag ccgacctgag agggtgatcg 300 gccacattgg gactgagaca cggcccaaac tcctacggga ggcagcagta gggaatcttc 360 ggcaatggac gaaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt ttcggatcgt 420 aaaactctgt tgttagagaa gaacaaggat gagagtaact gttcatccct tgacggtatc 480 taaccagaaa gccacggcta actacgtgcc agcagccgcg gtaatacgta ggtggcaagc 540 gttgtccgga tttattgggc gtaaagcgag cgcaggcggt ttcttaagtc tgatgtgaaa 600 gcccccggct caaccgggga gggtcattgg aaactgggag acttgagtgc agaagaggag 660 agtggaattc catgtgtagc ggtgaaatgc gtagatatat ggaggaacac cagtggcgaa 720 ggcggctctc tggtctgtaa ctgacgctga ggctcgaaag cgtggggagc aaacaggatt 780 agataccctg gtagtccacg ccgtaaacga tgagtgctaa gtgttggagg gtttccgccc 840 ttcagtgctg cagctaacgc attaagcact ccgcctgggg agtacgaccg caaggttgaa 900 actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca 960 acgcgaagaa ccttaccagg tcttgacatc ctttgaccac tctagagata gagcttcccc 1020 ttcgggggca aagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg 1080 gttaagtccc gcaacgagcg caacccttat tgttagttgc catcatttag ttgggcactc 1140 tagcaagact gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc 1200 cttatgacct gggctacaca cgtgctacaa tgggaagtac aacgagtcgc aaagtcgcga 1260 ggctaagcta atctcttaaa gcttctctca gttcggattg taggctgcaa ctcgcctaca 1320 tgaagccgga atcgctagta atcgcggatc agcacgccgc ggtgaatacg ttcccgggcc 1380 t 1381

<210> 175

<211> 1379

<212> DNA

<213> Enterococcus faecium 6

<400> 175 ctccaccgga aaaagaggag tggcgaacgg gtgagtaaca cgtgggtaac ctgcccatca 60 gaaggggata acacttggaa acaggtgcta ataccgtata acaatcaaaa ccgcatggtt 120 ttgatttgaa aggcgctttc gggtgtcgct gatggatgga cccgcggtgc attagctagt 180 tggtgaggta acggctcacc aaggccacga tgcatagccg acctgagagg gtgatcggcc 240 acattgggac tgagacacgg cccaaactcc tacgggaggc agcagtaggg aatcttcggc 300 aatggacgaa agtctgaccg agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa 360 actctgttgt tagagaagaa caaggatgag agtaactgtt catcccttga cggtatctaa 420 ccagaaagcc acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt 480 gtccggattt attgggcgta aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc 540 cccggctcaa ccggggaggg tcattggaaa ctgggagact tgagtgcaga agaggagagt 600 ggaattccat gtgtagcggt gaaatgcgta gatatatgga ggaacaccag tggcgaaggc 660 ggctctctgg tctgtaactg acgctgaggc tcgaaagcgt ggggagcaaa caggattaga 720 taccctggta gtccacgccg taaacgatga gtgctaagtg ttggagggtt tccgcccttc 780 agtgctgcag ctaacgcatt aagcactccg cctggggagt acgaccgcaa ggttgaaact 840 caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg 900 cgaagaacct taccaggtct tgacatcctt tgaccactct agagatagag cttccccttc 960 gggggcaaag tgacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt 1020 aagtcccgca acgagcgcaa cccttattgt tagttgccat cattcagttg ggcactctag 1080 caagactgcc ggtgacaaac cggaggaagg tggggatgac gtcaaatcat catgcccctt 1140 atgacctggg ctacacacgt gctacaatgg gaagtacaac gagttgcgaa gtcgcgaggc 1200 taagctaatc tcttaaagct tctctcagtt cggattgcag gctgcaactc gcctgcatga 1260 agccggaatc gctagtaatc gcggatcagc acgccgcggt gaatacgttc ccgggccttg 1320 tacacaccgc ccgtcacacc acgagagttt gtaacacccg aagtcggtga ggtaacctt 1379

<210> 176

<211> 1379

<212> DNA

<213> Enterococcus faecium 7

<400> 176 ctccaccgga aaaagaggag tggcgaacgg gtgagtaaca cgtgggtaac ctgcccatca 60 gaaggggata acacttggaa acaggtgcta ataccgtata acaatcaaaa ccgcatggtt 120 ttgatttgaa aggcgctttc gggtgtcgct gatggatgga cccgcggtgc attagctagt 180 tggtgaggta acggctcacc aaggccacga tgcatagccg acctgagagg gtgatcggcc 240 acattgggac tgagacacgg cccaaactcc tacgggaggc agcagtaggg aatcttcggc 300 aatggacgaa agtctgaccg agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa 360 actctgttgt tagagaagaa caaggatgag agtaactgtt catcccttga cggtatctaa 420 ccagaaagcc acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt 480 gtccggattt attgggcgta aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc 540 cccggctcaa ccggggaggg tcattggaaa ctgggagact tgagtgcaga agaggagagt 600 ggaattccat gtgtagcggt gaaatgcgta gatatatgga ggaacaccag tggcgaaggc 660 ggctctctgg tctgtaactg acgctgaggc tcgaaagcgt ggggagcaaa caggattaga 720 taccctggta gtccacgccg taaacgatga gtgctaagtg ttggagggtt tccgcccttc 780 agtgctgcag ctaacgcatt aagcactccg cctggggagt acgaccgcaa ggttgaaact 840 caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg 900 cgaagaacct taccaggtct tgacatcctt tgaccactct agagatagag cttccccttc 960 gggggcaaag tgacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt 1020 aagtcccgca acgagcgcaa cccttattgt tagttgccat cattcagttg ggcactctag 1080 caagactgcc ggtgacaaac cggaggaagg tggggatgac gtcaaatcat catgcccctt 1140 atgacctggg ctacacacgt gctacaatgg gaagtacaac gagttgcgaa gtcgcgaggc 1200 taagctaatc tcttaaagct tctctcagtt cggattgcag gctgcaactc gcctgcatga 1260 agccggaatc gctagtaatc gcggatcagc acgccgcggt gaatacgttc ccgggccttg 1320 tacacaccgc ccgtcacacc acgagagttt gtaacacccg aagtcggtga ggtaacctt 1379

<210> 177

<211> 1379

<212> DNA

<213> Enterococcus faecium 8

<400> 177 ctccaccgga aaaagaggag tggcgaacgg gtgagtaaca cgtgggtaac ctgcccatca 60 gaaggggata acacttggaa acaggtgcta ataccgtata acaatcaaaa ccgcatggtt 120 ttgatttgaa aggcgctttc gggtgtcgct gatggatgga cccgcggtgc attagctagt 180 tggtgaggta acggctcacc aaggccacga tgcatagccg acctgagagg gtgatcggcc 240 acattgggac tgagacacgg cccaaactcc tacgggaggc agcagtaggg aatcttcggc 300 aatggacgaa agtctgaccg agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa 360 actctgttgt tagagaagaa caaggatgag agtaactgtt catcccttga cggtatctaa 420 ccagaaagcc acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt 480 gtccggattt attgggcgta aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc 540 cccggctcaa ccggggaggg tcattggaaa ctgggagact tgagtgcaga agaggagagt 600 ggaattccat gtgtagcggt gaaatgcgta gatatatgga ggaacaccag tggcgaaggc 660 ggctctctgg tctgtaactg acgctgaggc tcgaaagcgt ggggagcaaa caggattaga 720 taccctggta gtccacgccg taaacgatga gtgctaagtg ttggagggtt tccgcccttc 780 agtgctgcag ctaacgcatt aagcactccg cctggggagt acgaccgcaa ggttgaaact 840 caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg 900 cgaagaacct taccaggtct tgacatcctt tgaccactct agagatagag cttccccttc 960 gggggcaaag tgacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt 1020 aagtcccgca acgagcgcaa cccttattgt tagttgccat cattcagttg ggcactctag 1080 caagactgcc ggtgacaaac cggaggaagg tggggatgac gtcaaatcat catgcccctt 1140 atgacctggg ctacacacgt gctacaatgg gaagtacaac gagttgcgaa gtcgcgaggc 1200 taagctaatc tcttaaagct tctctcagtt cggattgcag gctgcaactc gcctgcatga 1260 agccggaatc gctagtaatc gcggatcagc acgccgcggt gaatacgttc ccgggccttg 1320 tacacaccgc ccgtcacacc acgagagttt gtaacacccg aagtcggtga ggtaacctt 1379

<210> 178 <211> 1379

<212> DNA

<213> Enterococcus faecium 9

<400> 178 ctccaccgga aaaagaggag tggcgaacgg gtgagtaaca cgtgggtaac ctgcccatca 60 gaaggggata acacttggaa acaggtgcta ataccgtata acaatcaaaa ccgcatggtt 120 ttgatttgaa aggcgctttc gggtgtcgct gatggatgga cccgcggtgc attagctagt 180 tggtgaggta acggctcacc aaggccacga tgcatagccg acctgagagg gtgatcggcc 240 acattgggac tgagacacgg cccaaactcc tacgggaggc agcagtaggg aatcttcggc 300 aatggacgaa agtctgaccg agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa 360 actctgttgt tagagaagaa caaggatgag agtaactgtt catcccttga cggtatctaa 420 ccagaaagcc acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt 480 gtccggattt attgggcgta aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc 540 cccggctcaa ccggggaggg tcattggaaa ctgggagact tgagtgcaga agaggagagt 600 ggaattccat gtgtagcggt gaaatgcgta gatatatgga ggaacaccag tggcgaaggc 660 ggctctctgg tctgtaactg acgctgaggc tcgaaagcgt ggggagcaaa caggattaga 720 taccctggta gtccacgccg taaacgatga gtgctaagtg ttggagggtt tccgcccttc 780 agtgctgcag ctaacgcatt aagcactccg cctggggagt acgaccgcaa ggttgaaact 840 caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg 900 cgaagaacct taccaggtct tgacatcctt tgaccactct agagatagag cttccccttc 960 gggggcaaag tgacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt 1020 aagtcccgca acgagcgcaa cccttattgt tagttgccat cattcagttg ggcactctag 1080 caagactgcc ggtgacaaac cggaggaagg tggggatgac gtcaaatcat catgcccctt 1140 atgacctggg ctacacacgt gctacaatgg gaagtacaac gagttgcgaa gtcgcgaggc 1200 taagctaatc tcttaaagct tctctcagtt cggattgcag gctgcaactc gcctgcatga 1260 agccggaatc gctagtaatc gcggatcagc acgccgcggt gaatacgttc ccgggccttg 1320 tacacaccgc ccgtcacacc acgagagttt gtaacacccg aagtcggtga ggtaacctt 1379

<210> 179

<211> 1366

<212> DNA

<213> Enterococcus mundtii

<400> 179 agaggagtgg cgaacgggtg agtaacacgt gggtaacctg cccatcagaa ggggataaca 60 cttggaaaca ggtgctaata ccgtataaca atcgaaaccg catggtttcg ttttgaaagg 120 cgctttacgg tgccgctgat ggatggaccc gcggtgcatt agctagttgg tgaggtaacg 180 gctcaccaag gccacgatgc atagccgacc tgagagggtg atcggccaca ttgggactga 240 gacacggccc aaactcctac gggaggcagc agtagggaat cttcggcaat ggacgaaagt 300 ctgaccgagc aacgccgcgt gagtgaagaa ggttttcgga tcgtaaaact ctgttgttag 360 agaagaacaa gggtgagagt aactgttcac cccttgacgg tatctaacca gaaagccacg 420 gctaactacg tgccagcagc cgcggtaata cgtaggtggc aagcgttgtc cggatttatt 480 gggcgtaaag cgagcgcagg cggtttctta agtctgatgt gaaagccccc ggctcaaccg 540 gggagggtca ttggaaactg ggagacttga gtgcagaaga ggagagtgga attccatgtg 600 tagcggtgaa atgcgtagat atatggagga acaccagtgg cgaaggcggc tctctggtct 660 gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag gattagatac cctggtagtc 720 cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc gcccttcagt gctgcagcta 780 acgcattaag cactccgcct ggggagtacg accgcaaggt tgaaactcaa aggaattgac 840 gggggcccgc acaagcggtg gagcatgtgg tttaattcga agcaacgcga agaaccttac 900 caggtcttga catcctttga ccactctaga gatagagctt ccccttcggg ggcaaagtga 960 caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg 1020 agcgcaaccc ttattgttag ttgccatcat ttagttgggc actctagcaa gactgccggt 1080 gacaaaccgg aggaaggtgg gggatgacgt caaatcatca tgccccttat gacctgggct 1140 acacacgtgc tacaatggga agtacaacga gtcgcgaagt cgcgaggcta agctaatctc 1200 ttaaagcttc tctcagttcg gattgtaggc tgcaactcgc ctacatgaag ccggaatcgc 1260 tagtaatcgc ggatcagcac gccgcgggtg aaaaacgttc ccggggcctt gtacacaccc 1320 gcccgtcaca ccacggagag tttgtaacac cccgaagtcg gtgaag 1366

<210> 180

<211> 1362

<212> DNA

<213> Enterococcus mundtii 2

<400> 180 agaggagtgg cgaacgggtg agtaacacgt gggtaacctg cccatcagaa ggggataaca 60 cttggaaaca ggtgctaata ccgtataaca atcgaaaccg catggtttcg ttttgaaagg 120 cgctttacgg tgccgctgat ggatggaccc gcggtgcatt agctagttgg tgaggtaacg 180 gctcaccaag gccacgatgc atagccgacc tgagagggtg atcggccaca ttgggactga 240 gacacggccc aaactcctac gggaggcagc agtagggaat cttcggcaat ggacgaaagt 300 ctgaccgagc aacgccgcgt gagtgaagaa ggttttcgga tcgtaaaact ctgttgttag 360 agaagaacaa gggtgagagt aactgttcac cccttgacgg tatctaacca gaaagccacg 420 gctaactacg tgccagcagc cgcggtaata cgtaggtggc aagcgttgtc cggatttatt 480 gggcgtaaag cgagcgcagg cggtttctta agtctgatgt gaaagccccc ggctcaaccg 540 gggagggtca ttggaaactg ggagacttga gtgcagaaga ggagagtgga attccatgtg 600 tagcggtgaa atgcgtagat atatggagga acaccagtgg cgaaggcggc tctctggtct 660 gtaaactgac gctgaggctc gaaagcgtgg ggagcaaaca ggattagata ccctggtagt 720 ccacgccgta aacgatgagt gctaagtgtt ggagggtttc cgcccttcag tgctgcagct 780 aacgcattaa gcactccgcc tggggagtac gaccgcaagg ttgaaactca aaggaattga 840 cgggggcccg cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg aagaacctta 900 ccaggtcttg acatcctttg accactctag agatagagct tccccttcgg gggcaaagtg 960 acaggtggtg catggttgtc gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac 1020 gagcgcaacc cttattgtta gttgccatca tttagttggg cactctagca agactgccgg 1080 tgacaaaccg gaggaaggtg ggggatgacg tcaaatcatc atgcccctta tgacctgggc 1140 tacacacgtg ctacaatggg aagtacaacg agtcgcgaag tcgcgaggct aagctaatct 1200 cttaaagctt ctctcagttc ggattgtagg ctgcaactcg cctacatgaa gccggaatcg 1260 ctagtaatcg cggatcacac gccgcgggga aaaacctttc ccgggccttg tacacaccgc 1320 ccgtcacacc accgagagtt tgtaacaccc gaagtcggtg ag 1362

<210> 181

<211> 1361

<212> DNA

<213> Enterococcus mundtii 3

<400> 181 agaggagtgg cgaacgggtg agtaacacgt gggtaacctg cccatcagaa ggggataaca 60 cttggaaaca ggtgctaata ccgtataaca atcgaaaccg catggtttcg ttttgaaagg 120 cgctttacgg tgccgctgat ggatggaccc gcggtgcatt agctagttgg tgaggtaacg 180 gctcaccaag gccacgatgc atagccgacc tgagagggtg atcggccaca ttgggactga 240 gacacggccc aaactcctac gggaggcagc agtagggaat cttcggcaat ggacgaaagt 300 ctgaccgagc aacgccgcgt gagtgaagaa ggttttcgga tcgtaaaact ctgttgttag 360 agaagaacaa gggtgagagt aactgttcac cccttgacgg tatctaacca gaaagccacg 420 gctaactacg tgccagcagc cgcggtaata cgtaggtggc aagcgttgtc cggatttatt 480 gggcgtaaag cgagcgcagg cggtttctta agtctgatgt gaaagccccc ggctcaaccg 540 gggagggtca ttggaaactg ggagacttga gtgcagaaga ggagagtgga attccatgtg 600 tagcggtgaa atgcgtagat atatggagga acaccagtgg cgaaggcggc tctctggtct 660 gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag gattagatac cctggtagtc 720 cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc gcccttcagt gctgcagcta 780 acgcattaag cactccgcct ggggagtacg accgcaaggt tgaaactcaa aggaattgac 840 gggggcccgc acaagcggtg gagcatgtgg tttaattcga agcaacgcga agaaccttac 900 caggtcttga catcctttga ccactctaga gatagagctt ccccttcggg ggcaaagtga 960 caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg 1020 agcgcaaccc ttattgttag ttgccatcat ttagttgggc actctagcaa gactgccggt 1080 gacaaaccgg aggaaggtgg ggatgacgtc aaatcatcat gccccttatg acctgggcta 1140 cacacgtgct acaatgggaa gtacaacgag tcgcgaagtc gcgaggctaa gctaatctct 1200 taaagcttct ctcagttcgg attgtaggct gcaactcgcc tacatgaagc cggaatcgct 1260 agtaatcgcg gatcagcacg ccgcgggtga atacgtttcc cgggccttgt acacaccgcc 1320 cgtcacacca cgaagagttt gtaacacccg aagtcggtga g 1361

<210> 182

<211> 1359

<212> DNA

<213> Enterococcus faecium 10

<400> 182 agaaggggat aacacttgga aacaggtgct aataccgtat aacaatcgaa accgcatggt 60 tttgatttga aaggcgcttt cgggtgtcgc tgatggatgg acccgcggtg cattagctag 120 ttggtgaggt aacggctcac caaggccacg atgcatagcc gacctgagag ggtgatcggc 180 cacattggga ctgagacacg gcccaaactc ctacgggagg cagcagtagg gaatcttcgg 240 caatggacga aagtctgacc gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa 300 aactctgttg ttagagaaga acaaggatga gagtaactgt tcatcccttg acggtatcta 360 accagaaagc cacggctaac tacgtgccag cagccgcggt aatacgtagg tggcaagcgt 420 tgtccggatt tattgggcgt aaagcgagcg caggcggttt cttaagtctg atgtgaaagc 480 ccccggctca accggggagg gtcattggaa actgggagac ttgagtgcag aagaggagag 540 tggaattcca tgtgtagcgg tgaaatgcgt agatatatgg aggaacacca gtggcgaagg 600 cggctctctg gtctgtaact gacgctgagg ctcgaaagcg tggggagcaa acaggattag 660 ataccctggt agtccacgcc gtaaacgatg agtgctaagt gttggagggt ttccgccctt 720 cagtgctgca gctaacgcat taagcactcc gcctggggag tacgaccgca aggttgaaac 780 tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac 840 gcgaagaacc ttaccaggtc ttgacatcct ttgaccactc tagagataga gcttcccctt 900 cgggggcaaa gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt 960 taagtcccgc aacgagcgca acccttattg ttagttgcca tcattcagtt gggcactcta 1020 gcaagactgc cggtgacaaa ccggaggaag gtggggatga cgtcaaatca tcatgcccct 1080 tatgacctgg gctacacacg tgctacaatg ggaagtacaa cgagttgcga agtcgcgagg 1140 ctaagctaat ctcttaaagc ttctctcagt tcggattgca ggctgcaact cgcctgcatg 1200 aagccggaat cgctagtaat cgcggatcag cacgccgcgg tgaatacgtt cccgggcctt 1260 gtacacaccg cccgtcacac cacgagagtt tgtaacaccc gaagtcggtg aggtaacctt 1320 tggagccagc cgcctaaggt ggatagatga tgggggaag 1359

<210> 183

<211> 1340

<212> DNA

<213> Enterococcus faecium 11

<400> 183 ctttttccac cggagcttgc tccaccggaa aaagaggagt ggcgaacggg gtgagtaaca 60 cgtgggtaac ctgcccatca gaaggggata acacttggaa acaggtgcta ataccgtata 120 acaatcgaaa ccgcatggtt ttgatttgaa aggcgctttc gggtgtcgct gatggatgga 180 cccgcggtgc attagctagt tggtgaggta acggctcacc aaggccacga tgcatagccg 240 acctgagagg gtgatcggcc acattgggac tgagacacgg cccaaactcc tacgggaggc 300 agcagtaggg aatcttcggc aatggacgaa agtctgaccg agcaacgccg cgtgagtgaa 360 gaaggttttc ggatcgtaaa actctgttgt tagagaagaa caaggatgag agtaactgtt 420 catcccttga cggtatctaa ccagaaagcc acggctaact acgtgccagc agccgcggta 480 atacgtaggt ggcaagcgtt gtccggattt attgggcgta aagcgagcgc aggcggtttc 540 ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg tcattggaaa ctgggagact 600 tgagtgcaga agaggagagt ggaattccat gtgtagcggt gaaatgcgta gatatatgga 660 ggaacaccag tggcgaaggc ggctctctgg tctgtaactg acgctgaggc tcgaaagcgt 720 ggggagcaaa caggattaga taccctggta gtccacgccg taaacgatga gtgctaagtg 780 ttggagggtt tccgcccttc agtgctgcag ctaacgcatt aagcactccg cctggggagt 840 acgaccgcaa ggttgaaact caaaggaatt gacgggggcc cgcacaagcg gtggagcatg 900 tggtttaatt cgaagcaacg cgaagaacct taccaggtct tgacatcctt tgaccactct 960 agagatagag cttccccttc gggggcaaag tgacaggtgg tgcatggttg tcgtcagctc 1020 gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa cccttattgt tagttgccat 1080 cattcagttg ggcactctag caagactgcc ggtgacaaac cggaggaagg tggggatgac 1140 gtcaaatcat catgcccctt atgacctggg ctacacacgt gctacaatgg gaagtacaac 1200 gagttgcgaa gtcgcgaggc taagctaatc tcttaaagct tctctcagtt cggattgcag 1260 gctgcaactc gcctgcatga agccggaatc gctagtaatc gcggatcagc acgccgcggt 1320 gaatacgttc ccgggccttg 1340 <210> 184

<211> 1339

<212> DNA

<213> Enterococcus faecium 12

<400> 184 aaaaagaaga gtggcgaacg ggtgagtaac acgtgggtaa cctgcccatc agaaggggat 60 aacacttgga aacaggtgct aataccgtat aacaatcgaa accgcatggt tttgatttga 120 aaggcgcttt cgggtgtcgc tgatggatgg acccgcggtg cattagctag ttggtgaggt 180 aacggctcac caaggccacg atgcatagcc gacctgagag ggtgatcggc cacattggga 240 ctgagacacg gcccaaactc ctacgggagg cagcagtagg gaatcttcgg caatggacga 300 aagtctgacc gagcaacgcc gcgtgagtga agaaggtttt cggatcgtaa aactctgttg 360 ttagagaaga acaaggatga gagtaactgt tcatcccttg acggtatcta accagaaagc 420 cacggctaac tacgtgccag cagccgcggt aatacgtagg tggcaagcgt tgtccggatt 480 tattgggcgt aaagcgagcg caggcggttt cttaagtctg atgtgaaagc ccccggctca 540 accggggagg gtcattggaa actgggagac ttgagtgcag aagaggagag tggaattcca 600 tgtgtagcgg tgaaatgcgt agatatatgg aggaacacca gtggcgaagg cggctctctg 660 gtctgtaact gacgctgagg ctcgaaagcg tggggagcaa acaggattag ataccctggt 720 agtccacgcc gtaaacgatg agtgctaagt gttggagggt ttccgccctt cagtgctgca 780 gctaacgcat taagcactcc gcctggggag tacgaccgca aggttgaaac tcaaaggaat 840 tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc 900 ttaccaggtc ttgacatcct ttgaccactc tagagataga gcttcccctt cgggggcaaa 960 gtgacaggtg gtgcatggtt gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc 1020 aacgagcgca acccttattg ttagttgcca tcattcagtt gggcactcta gcaagactgc 1080 cggtgacaaa ccggaggaag gtggggatga cgtcaaatca tcatgcccct tatgacctgg 1140 gctacacacg tgctacaatg ggaagtacaa cgagttgcga agtcgcgagg ctaagctaat 1200 ctcttaaagc ttctctcagt tcggattgca ggctgcaact cgcctgcatg aagccggaat 1260 cgctagtaat cgcggatcag cacgccgcgg tgaatacgtt cccgggcctt gtacacaccg 1320 cccgtcacac cacgagagt 1339

<210> 185

<211> 1337

<212> DNA

<213> Enterococcus sp. LV 07

<400> 185 tcttttccca ccggagcttg ctccaggggg taaaagagga gtggcgaacg ggtgagtaac 60 acgtgggtaa cctgcccatc agaaggggat aacacttgga aacaggtgct aataccgtat 120 aacaatcgaa accgcatggt ttcgttttga aaggcgcttt acggtgccgc tgatggatgg 180 acccgcggtg cattagctag ttggtgaggt aacggctcac caaggccacg atgcatagcc 240 gacctgagag ggtgatcggc cacattggga ctgagacacg gcccaaactc ctacgggagg 300 cagcagtagg gaatcttcgg caatggacga aagtctgacc gagcaacgcc gcgtgagtga 360 agaaggtttt cggatcgtaa aactctgttg ttagagaaga acaagggtga gagtaactgt 420 tcaccccttg acggtatcta accagaaagc cacggctaac tacgtgccag cagccgcggt 480 aatacgtagg tggcaagcgt tgtccggatt tattgggcgt aaagcgagcg caggcggttt 540 cttaagtctg atgtgaaagc ccccggctca accggggagg gtcattggaa actgggagac 600 ttgagtgcag aagaggagag tggaattcca tgtgtagcgg tgaaatgcgt agatatatgg 660 aggaacacca gtggcgaagg cggctctctg gtctgtaact gacgctgagg ctcgaaagcg 720 tggggagcaa acaggattag ataccctggt agtccacgcc gtaaacgatg agtgctaagt 780 gttggagggt ttccgccctt cagtgctgca gctaacgcat taagcactcc gcctggggag 840 tacgaccgca aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat 900 gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc ttgacatcct ttgaccactc 960 tagagataga gcttcccctt cgggggcaaa gtgacaggtg gtgcatggtt gtcgtcagct 1020 cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca acccttattg ttagttgcca 1080 tcatttagtt gggcactcta gcaagactgc cggtgacaaa ccggaggaag gtggggatga 1140 cgtcaaatca tcatgcccct tatgacctgg gctacacacg tgctacaatg ggaagtacaa 1200 cgagtcgcga agtcgcgagg ctaagctaat ctcttaaagc ttctctcagt tcggattgta 1260 ggctgcaact cgccacatga agccggaatc gctagtaatc gcggatcagc acgccgcggt 1320 gaatacgttc ccgggcc 1337

<210> 186

<211> 1335

<212> DNA

<213> Enterococcus hirae 7

<400> 186 gagtggcgaa cgggtgagta acacgtgggt aacctgccca tcagaagggg ataacacttg 60 gaaacaggtg ctaataccgt ataacaatcg aaaccgcatg gttttgattt gaaaggcgct 120 ttcgggtgtc gctgatggat ggacccgcgg tgcattagct agttggtgag gtaacggctc 180 accaaggcga cgatgcatag ccgacctgag agggtgatcg gccacattgg gactgagaca 240 cggcccaaac tcctacggga ggcagcagta gggaatcttc ggcaatggac gaaagtctga 300 ccgagcaacg ccgcgtgagt gaagaaggtt ttcggatcgt aaaactctgt tgttagagaa 360 gaacaaggat gagagtaact gttcatccct tgacggtatc taaccagaaa gccacggcta 420 actacgtgcc agcagccgcg gtaatacgta ggtggcaagc gttgtccgga tttattgggc 480 gtaaagcgag cgcaggcggt ttcttaagtc tgatgtgaaa gcccccggct caaccgggga 540 gggtcattgg aaactgggag acttgagtgc agaagaggag agtggaattc catgtgtagc 600 ggtgaaatgc gtagatatat ggaggaacac cagtggcgaa ggcggctctc tggtctgtaa 660 ctgacgctga ggctcgaaag cgtggggagc aaacaggatt agataccctg gtagtccacg 720 ccgtaaacga tgagtgctaa gtgttggagg gtttccgccc ttcagtgctg cagctaacgc 780 attaagcact ccgcctgggg agtacgaccg caaggttgaa actcaaagga attgacgggg 840 gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgaagaa ccttaccagg 900 tcttgacatc ctttgaccac tctagagata gagcttcccc ttcgggggca aagtgacagg 960 tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg 1020 caacccttat tgttagttgc catcatttag ttgggcactc tagcaagact gccggtgaca 1080 aaccggagga aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac 1140 acgtgctaca atgggaagta caacgagtcg caaagtcgcg aggctaagct aatctcttaa 1200 agcttctctc agttcggatt gtaggctgca actcgcctac atgaagccgg aatcgctagt 1260 aatcgcggat cagcacgccg cggtgaatac gttcccgggc cttgtacaca ccgcccgtca 1320 caccacgaga gtttg 1335

<210> 187

<211> 1334

<212> DNA

<213> Enterococcus faecium 13

<400> 187 ctccaccgga aaaagaggag tggcgaacgg gtgagtaaca cgtgggtaac ctgcccatca 60 gaaggggata acacttggaa acaggtgcta ataccgtata acaatcaaaa ccgcatggtt 120 ttgatttgaa aggcgctttc gggtgtcgct gatggatgga cccgcggtgc attagctagt 180 tggtgaggta acggctcacc aaggccacga tgcatagccg acctgagagg gtgatcggcc 240 acattgggac tgagacacgg cccaaactcc tacgggaggc agcagtaggg aatcttcggc 300 aatggacgaa agtctgaccg agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa 360 actctgttgt tagagaagaa caaggatgag agtaactgtt catcccttga cggtatctaa 420 ccagaaagcc acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt 480 gtccggattt attgggcgta aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc 540 cccggctcaa ccggggaggg tcattggaaa ctgggagact tgagtgcaga agaggagagt 600 ggaattccat gtgtagcggt gaaatgcgta gatatatgga ggaacaccag tggcgaaggc 660 ggctctctgg tctgtaactg acgctgaggc tcgaaagcgt ggggagcaaa caggattaga 720 taccctggta gtccacgccg taaacgatga gtgctaagtg ttggagggtt tccgcccttc 780 agtgctgcag ctaacgcatt aagcactccg cctggggagt acgaccgcaa ggttgaaact 840 caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg 900 cgaagaacct taccaggtct tgacatcctt tgaccactct agagatagag cttccccttc 960 gggggcaaag tgacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt 1020 aagtcccgca acgagcgcaa cccttattgt tagttgccat cattcagttg ggcactctag 1080 caagactgcc ggtgacaaac cggaggaagg tggggatgac gtcaaatcat catgcccctt 1140 atgacctggg ctacacacgt gctacaatgg gaagtacaac gagttgcgaa gtcgcgaggc 1200 taagctaatc tcttaaagct tctctcagtt cggattgcag gctgcaactc gcctgcatga 1260 agccggaatc gctagtaatc gcggatcagc acgccgcggt gaatacgttc ccgggccttg 1320 tacacaccgc ccgt 1334

<210> 188

<211 > 1334

<212> DNA

<213> Enterococcus faecium 14

<400> 188 ccaccggagc ttgctccacc ggaaaaagag gagtggcgaa cgggtgagta acacgtgggt 60 aacctgccca tcagaagggg ataacacttg gaaacaggtg ctaataccgt ataacaatca 120 aaaccgcatg gttttgattt gaaaggcgct ttcgggtgtc gctgatggat ggacccgcgg 180 tgcattagct agttggtgag gtaacggctc accaaggcca cgatgcatag ccgacctgag 240 agggtgatcg gccacattgg gactgagaca cggcccaaac tcctacggga ggcagcagta 300 gggaatcttc ggcaatggac gaaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt 360 ttcggatcgt aaaactctgt tgttagagaa gaacaaggat gagagtaact gttcatccct 420 tgacggtatc taaccagaaa gccacggcta actacgtgcc agcagccgcg gtaatacgta 480 ggtggcaagc gttgtccgga tttattgggc gtaaagcgag cgcaggcggt ttcttaagtc 540 tgatgtgaaa gcccccggct caaccgggga gggtcattgg aaactgggag acttgagtgc 600 agaagaggag agtggaattc catgtgtagc ggtgaaatgc gtagatatat ggaggaacac 660 cagtggcgaa ggcggctctc tggtctgtaa ctgacgctga ggctcgaaag cgtggggagc 720 aaacaggatt agataccctg gtagtccacg ccgtaaacga tgagtgctaa gtgttggagg 780 gtttccgccc ttcagtgctg cagctaacgc attaagcact ccgcctgggg agtacgaccg 840 caaggttgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc atgtggttta 900 attcgaagca acgcgaagaa ccttaccagg tcttgacatc ctttgaccac tctagagata 960 gagcttcccc ttcgggggca aagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt 1020 gagatgttgg gttaagtccc gcaacgagcg caacccttat tgttagttgc catcattcag 1080 ttgggcactc tagcaagact gccggtgaca aaccggagga aggtggggat gacgtcaaat 1140 catcatgccc cttatgacct gggctacaca cgtgctacaa tgggaagtac aacgagtcgc 1200 gaagtcgcga ggctaagcta atctcttaaa gcttctctca gttcggattg caggctgcaa 1260 ctcgcctgca tgaagccgga atcgctagta atcgcggatc agcacgccgc ggtgaatacg 1320 ttcccgggcc ttgt 1334

<210> 189

<211> 1320

<212> DNA

<213> Enterococcus mundtii QU 25

<400> 189 tggcgaacgg gtgagtaaca cgtgggtaac ctgcccatca gaaggggata acacttggaa 60 acaggtgcta ataccgtata acaatcgaaa ccgcatggtt tcgttttgaa aggcgcttta 120 cggtgccgct gatggatgga cccgcggtgc attagctagt tggtgaggta acggctcacc 180 aaggccacga tgcatagccg acctgagagg gtgatcggcc acattgggac tgagacacgg 240 cccaaactcc tacgggaggc agcagtaggg aatcttcggc aatggacgaa agtctgaccg 300 agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt tagagaagaa 360 caagggtgag agtaactgtt caccccttga cggtatctaa ccagaaagcc acggctaact 420 acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggattt attgggcgta 480 aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg 540 tcattggaaa ctgggagact tgagtgcaga agaggagagt ggaattccat gtgtagcggt 600 gaaatgcgta gatatatgga ggaacaccag tggcgaaggc ggctctctgg tctgtaactg 660 acgctgaggc tcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccg 720 taaacgatga gtgctaagtg ttggagggtt tccgcccttc agtgctgcag ctaacgcatt 780 aagcactccg cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc 840 cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaggtct 900 tgacatcctt tgaccactct agagatagag cttccccttc gggggcaaag tgacaggtgg 960 tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa 1020 cccttattgt tagttgccat catttagttg ggcactctag caagactgcc ggtgacaaac 1080 cggaggaagg tggggatgac gtcaaatcat catgcccctt atgacctggg ctacacacgt 1140 gctacaatgg gaagtacaac gagtcgcgaa gtcgcgaggc taagctaatc ttttaaagct 1200 tctctcagtt cggattgtag gctgcaactc gcctacatga agccggaatc gctagtaatc 1260 gcggatcagc acgccgcggt gaatacgttc ccgggccttg tacacaccgc ccgtcacacc 1320

<210> 190

<211> 1265

<212> DNA

<213> Prior Sequence 27

<400> 190 ggaaaaagag gagtggcgaa cgggtgagta acacgtgggt aacctgccca tcagaagggg 60 ataacacttg gaaacaggtg ctaataccgt ataacaatca aaaccgcatg gttttgattt 120 gaaaggcgct ttcgggtgtc gctgatggat ggacccgcgg tgcattagct agttggtgag 180 gtaacggctc accaaggcca cgatgcatag ccgacctgag agggtgatcg gccacattgg 240 gactgagaca cggcccaaac tcctacggga ggcagcagta gggaatcttc ggcaatggac 300 gaaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt ttcggatcgt aaaactctgt 360 tgttagagaa gaacaaggat gagagtaact gttcatccct tgacggtatc taaccagaaa 420 gccacggcta actacgtgcc agcagccgcg gtaatacgta ggtggcaagc gttgtccgga 480 tttattgggc gtaaagcgag cgcaggcggt ttcttaagtc tgatgtgaaa gcccccggct 540 caaccgggga gggtcattgg aaactgggag acttgagtgc agaagaggag agtggaattc 600 catgtgtagc ggtgaaatgc gtagatatat ggaggaacac cagtggcgaa ggcggctctc 660 tggtctgtaa ctgacgctga ggctcgaaag cgtggggagc aaacaggatt agataccctg 720 gtagtccacg ccgtaaacga tgagtgctaa gtgttggagg gtttccgccc ttcagtgctg 780 cagctaacgc attaagcact ccgcctgggg agtacgaccg caaggttgaa actcaaagga 840 attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgaagaa 900 ccttaccagg tcttgacatc ctttgaccac tctagagata gagcttcccc ttcgggggca 960 aagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc 1020 gcaacgagcg caacccttat tgttagttgc catcattcag ttgggcactc tagcaagact 1080 gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct 1140 gggctacaca cgtgctacaa tgggaagtac aacgagttgc gaagtcgcga ggctaagcta 1200 atctcttaaa gcttctctca gttcggattg caggctgcaa ctcgcctgca tgaagccgga 1260 atcgc 1265

<210> 191

<211> 1165

<212> DNA

<213> Enterococcus faecium 15

<400> 191 ggcgtgccta atacatgcaa gtcgaacgct tctttttcca ccggagcttg ctccaccgga 60 aaaagaggag tggcgaacgg gtgagtaaca cgtgggtaac ctgcccatca gaaggggata 120 acacttggaa acaggtgcta ataccgtata acaatcaaaa ccgcatggtt ttgatttgaa 180 aggcgctttc gggtgtcgct gatggatgga cccgcggtgc attagctagt tggtgaggta 240 acggctcacc aaggccacga tgcatagccg acctgagagg gtgatcggcc acattgggac 300 tgagacacgg cccaaactcc tacgggaggc agcagtaggg aatcttcggc aatggacgaa 360 agtctgaccg agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt 420 tagagaagaa caaggatgag agtaactgtt catcccttga cggtatctaa ccagaaagcc 480 acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggattt 540 attgggcgta aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa 600 ccggggaggg tcattggaaa ctgggagact tgagtgcaga agaggagagt ggaattccat 660 gtgtagcggt gaaatgcgta gatatatgga ggaacaccag tggcgaaggc ggctctctgg 720 tctgtaactg acgctgaggc tcgaaagcgt ggggagcaaa caggattaga taccctggta 780 gtccacgccg taaacgatga gtgctaagtg ttggagggtt tccgcccttc agtgctgcag 840 ctaacgcatt aagcactccg cctggggagt acgaccgcaa ggttgaaact caaaggaatt 900 gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg cgaagaacct 960 taccaggtct tgacatcctt tgaccactct agagatagag cttccccttc gggggcaaag 1020 tgacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca 1080 acgagcgcaa cccttattgt tagttgccat cattcagttg ggcactctag caagactgcc 1140 ggtgacaaac cggaggaagg tgggg 1165

<210> 192

<211> 1148

<212> DNA

<213> Enterococcus sp. 618B2_12AGalle

<400> 192 acatgcaagt cgaacgcttc tttttccacc ggagcttgct ccaccggaaa aagaggagtg 60 gcgaacgggt gagtaacacg tgggtaacct gcccatcaga aggggataac acttggaaac 120 aggtgctaat accgyataac aatcaaaacc gcatggtttt gatttgaaag gcgctttcgg 180 gtgtcgctga tggatggacc cgcggtgcat tagctagttg gtgaggtaac ggctcaccaa 240 ggccacgatg catagccgac ctgagagggt gatcggccac attgggactg agacacggcc 300 caaactccta cgggaggcag cagtagggaa tcttcggcaa tggacgaaag tctgaccgag 360 caacgccgcg tgagtgaaga aggttttcgg atcgtaaaac tctgttgtta gagaagaaca 420 aggatgagag taactgttca tcccttgacg gtatctaacc agaaagccac ggctaactac 480 gtgccagcag ccgcggtaat acgtaggtgg caagcgttgt ccggatttat tgggcgtaaa 540 gcgagcgcag gcggtttctt aagtctgatg tgaaagcccc cggctcaacc ggggagggtc 600 attggaaact gggagacttg agtgcagaag aggagagtgg aattccatgt gtagcggtga 660 aatgcgtaga tatatggagg aacaccagtg gcgaaggcgg ctctctggtc tgtaactgac 720 gctgaggctc gaaagcgtgg ggagcaaaca ggattagata ccctggtagt ccacgccgta 780 aacgatgagt gctaagtgtt ggagggtttc cgcccttcag tgctgcagct aacgcattaa 840 gcactccgcc tggggagtac gaccgcaagg ttgaaactca aaggaattga cgggggcccg 900 cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg aagaacctta ccaggtcttg 960 acatcctttg accactctag agatagagct tccccttcgg gggcaaagtg acaggtggtg 1020 catggttgtc gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc 1080 cttattgtta gttgccatca ttcagttggg cactctagca agactgccgg tgacaaaccg 1140 gagaaagg 1148

<210> 193

<211> 1138

<212> DNA

<213> Enterococcus sp. 691 B1_12EGalle

<400> 193 gtcgaacgct tctttttcca ccggagcttg ctccaccgga aaaagaggag tggcgaacgg 60 gtgagtaaca cgtgggtaac ctgcccatca gaaggggata acacttggaa acaggtgcta 120 ataccgtata acaatcraaa ccgcatggtt ttgatttgaa aggcgctttc gggtgtcgct 180 gatggatgga cccgcggtgc attagctagt tggtgaggta acggctcacc aaggccacga 240 tgcatagccg acctgagagg gtgatcggcc acattgggac tgagacacgg cccaaactcc 300 tacgggaggc agcagtaggg aatcttcggc aatggacgaa agtctgaccg agcaacgccg 360 cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt tagagaagaa caaggatgag 420 agtaactgtt catcccttga cggtatctaa ccagaaagcc acggctaact acgtgccagc 480 agccgcggta atacgtaggt ggcaagcgtt gtccggattt attgggcgta aagcgagcgc 540 aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg tcattggaaa 600 ctgggagact tgagtgcaga agaggagagt ggaattccat gtgtagcggt gaaatgcgta 660 gatatatgga ggaacaccag tggcgaaggc ggctctctgg tctgtaactg acgctgaggc 720 tcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccg taaacgatga 780 gtgctaagtg ttggagggtt tccgcccttc agtgctgcag ctaacgcatt aagcactccg 840 cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc cgcacaagcg 900 gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaggtct tgacatcctt 960 tgaccactct agagatagag cttccccttc gggggcaaag tgacaggtgg tgcatggttg 1020 tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa cccttattgt 1080 tagttgccat cattcagttg ggcactctag caagactgcc ggtgacaaac cggaggaa 1138

<210> 194

<211> 1130

<212> DNA

<213> Enterococcus sp. 680B1_12ESB

<400> 194 gtcgaacgct tctttttcca ccggagcttg ctccaccgga aaaagaggag tggcgaacgg 60 gtgagtaaca cgtgggtaac ctgcccatca gaaggggata acacttggaa acaggtgcta 120 ataccgtata acaatcgaaa ccgcatggtt ttgatttgaa aggcgctttc gggtgtcgct 180 gatggatgga cccgcggtgc attagctagt tggtgaggta acggctcacc aaggccacga 240 tgcatagccg acctgagagg gtgatcggcc acattgggac tgagacacgg cccaaactcc 300 tacgggaggc agcagtaggg aatcttcggc aatggacgaa agtctgaccg agcaacgccg 360 cgtgagtgaa gaaggttttc ggatcgtaaa actctgttgt tagagaagaa caaggatgag 420 agtaactgtt catcccttga cggtatctaa ccagaaagcc acggctaact acgtgccagc 480 agccgcggta atacgtaggt ggcaagcgtt gtccggattt attgggcgta aagcgagcgc 540 aggcggtttc ttaagtctga tgtgaaagcc cccggctcaa ccggggaggg tcattggaaa 600 ctgggagact tgagtgcaga agaggagagt ggaattccat gtgtagcggt gaaatgcgta 660 gatatatgga ggaacaccag tggcgaaggc ggctctctgg tctgtaactg acgctgaggc 720 tcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccg taaacgatga 780 gtgctaagtg ttggagggtt tccgcccttc agtgctgcag ctaacgcatt aagcactccg 840 cctggggagt acgaccgcaa ggttgaaact caaaggaatt gacgggggcc cgcacaagcg 900 gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaggtct tgacatcctt 960 tgaccactct agagatagag cttccccttc gggggcaaag tgacaggtgg tgcatggttg 1020 tcgtcagctc gtgtcgtgag atgttgggtt aagtcccgca acgagcgcaa cccttattgt 1080 tagttgccat cattcagttg ggcactctag caagactgcc ggtgacaaac 1130

<210> 195

<211> 1126

<212> DNA

<213> Enterococcus sp. A3

<400> 195 tatagagttt gatccctggc tcaggacgaa cgctggcggc gtgcctaata catgcaagtc 60 gtacgctttt tctttcaccg gagcttgctc caccgaaaga aaaggagtgg cgaacgggtg 120 agtaacacgt gggtaacctg cccatcagaa ggggataaca cttggaaaca ggtgctaata 180 ccgtataaca atcgaaaccg catggttttg atttgaaagg cgctttcggg tgtcgctgat 240 ggatggaccc gcggtgcatt agctagttgg tgaggtaacg gctcaccaag gccacgatgc 300 atagccgacc tgagagggtg atcggccaca ttgggactga gacacggccc aaactcctac 360 gggaggcagc agtagggaat cttcggcaat ggacgaaagt ctgaccgagc aacgccgcgt 420 gagtgaagaa ggttttcgga tcgtaaaact ctgttgttag agaagaacaa ggatgagagt 480 aactgttcat cccttgacgg katctaacca gaaagccacg gctaactacg tgccagcagc 540 cgcggtaata cgtaggtggc aagcgttgtc cggatttatt gggcgtaaag cgagcgcagg 600 cggtttctta agtctgatgt gaaagccccc ggctcaaccg gggagggtca ttggaaactg 660 ggagacttga gtgcagaaga ggagagtgga attccatgtg tagcggtgaa atgcgtagat 720 atatggagga acaccagtgg cgaaggcgac tctctggtct gyaactgacg ctgaggctcg 780 aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa acgatgagtg 840 ctaagtgttg gagggtttcc gcccttcagt gctgcagcta acgcattaag cactccgcct 900 ggggagtacg accgcaaggt tgaaactcaa aggaattgac gggggcccgc acaagcggtg 960 gagcatgtgg tttaattcga agcaacgcga agaaccttac caggtcttga catcctttga 1020 ccactctaga gatagagctt ccccttcggg ggcaaagtga caggtggtgc atggttgtcg 1080 tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg agcgca 1126

<210> 196

<211> 1124

<212> DNA

<213> Enterococcus sp. 366B7_12EEMB

<400> 196 atgcaagtcg tacgcttctt tttccaccgg agcttgctcc accggaaaaa gaggagtggc 60 gaacgggtga gtaacacgtg ggtaacctgc ccatcagaag gggataacac ttggaaacag 120 gtgctaatac cgtataacaa tcgaaaccgc atggttttga tttgaaaggc gctttcgggt 180 gtcgctgatg gatggacccg cggtgcatta gctagttggt gaggtaacgg ctcaccaagg 240 ccacgatgca tagccgacct gagagggtga tcggccacat tgggactgag acacggccca 300 aactcctacg ggaggcagca gtagggaatc ttcggcaatg gacgaaagtc tgaccgagca 360 acgccgcgtg agtgaagaag gttttcggat cgtaaaactc tgttgttaga gaagaacaag 420 gatgagagta actgttcatc ccttgacggt atctaaccag aaagccacgg ctaactacgt 480 gccagcagcc gcggtaatac gtaggtggca agcgttgtcc ggatttattg ggcgtaaagc 540 gagcgcaggc ggtttcttaa gtctgatgtg aaagcccccg gctcaaccgg ggagggtcat 600 tggaaactgg gagacttgag tgcagaagag gagagtggaa ttccatgtgt agcggtgaaa 660 tgcgtagata tatggaggaa caccagtggc gaaggcggct ctctggtctg taactgacgc 720 tgaggctcga aagcgtgggg agcaaacagg attagatacc ctggtagtcc acgccgtaaa 780 cgatgagtgc taagtgttgg agggtttccg cccttcagtg ctgcagctaa cgcattaagc 840 actccgcctg gggagtacga ccgcaaggtt gaaactcaaa ggaattgacg ggggcccgca 900 caagcggtgg agcatgtggt ttaattcgaa gcaacgcgaa gaaccttacc aggtcttgac 960 atcctttgac cactctagag atagagcttc cccttcgggg gcaaagtgac aggtggtgca 1020 tggttgtcgt cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct 1080 tattgttagt tgccatcatt cagttgggca ctctagcaag actg 1124

<210> 197

<211> 1116 <212> DNA <213> Enterococcus sp. 545B2_12AVRE <400> 197 acatgcaagt cgaacgcttt ttctttcacc ggagcttgct ccaccgaaag aaaaagagtg 60 gcgaacgggt gagtaacacg tgggtaacct gcccatcaga aggggataac acttggaaac 120 aggtgctaat accgtataac actattttcc gcatggaaga aagttgaaag gcgcttttgc 180 gtcactgatg gatggacccg cggtgcatta gctagttggt gaggtaacgg ctcaccaagg 240 ccacgatgca tagccgacct gagagggtga tcggccacac tgggactgag acacggccca 300 gactcctacg ggaggcagca gtagggaatc ttcggcaatg gacgaaagtc tgaccgagca 360 acgccgcgtg agtgaagaag gttttcggat cgtaaaactc tgttgttaga gaagaacaag 420 gatgagagta gaacgttcat cccttgacgg tatctaacca gaaagccacg gctaactacg 480 tgccagcagc cgcggtaata cgtaggtggc aagcgttgtc cggatttatt gggcgtaaag 540 cgagcgcagg cggtttctta agtctgatgt gaaagccccc ggctcaaccg gggagggtca 600 ttggaaactg ggagacttga gtgcagaaga ggagagtgga attccatgtg tagcggtgaa 660 atgcgtagat atatggagga acaccagtgg cgaaggcggc tctctggtct gtaactgacg 720 ctgaggctcg aaagcgtggg gagcgaacag gattagatac cctggtagtc cacgccgtaa 780 acgatgagtg ctaagtgttg gagggtttcc gcccttcagt gctgcagcaa acgcattaag 840 cactccgcct ggggagtacg accgcaaggt tgaaactcaa aggaattgac gggggcccgc 900 acaagcggtg gagcatgtgg tttaattcga agcaacgcga agaaccttac caggtcttga 960 catcctttga ccactctaga gatagagctt ccccttcggg ggcaaagtga caggtggtgc 1020 atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg agcgcaaccc 1080 ttattgttag ttgccatcat ttagttgggc actcta 1116

<210> 198

<211> 1113

<212> DNA

<213> Enterococcus sp. 692B1_12AGalle <400> 198 cggagcttgc tccaccggag aaagaggagt ggcgaacggg tgagtaacac gtgggtaacc 60 tgcccatcag aaggggataa cacttggaaa caggtgctaa taccgtataa caatcgaaac 120 cgcatggttt tgatttgaaa ggcgctttcg ggtgtcgctg atggatggac ccgcggtgca 180 ttagctagtt ggtgaggtaa cggctcacca aggcgacgat gcatagccga cctgagaggg 240 tgatcggcca cattgggact gagacacggc ccaaactcct acgggaggca gcagtaggga 300 atcttcggca atggacgaaa gtctgaccga gcaacgccgc gtgagtgaag aaggttttcg 360 gatcgtaaaa ctctgttgtt agagaagaac aaggatgaga gtaactgttc atcccttgac 420 ggtatctaac cagaaagcca cggctaacta cgtgccagca gccgcggtaa tacgtaggtg 480 gcaagcgttg tccggattta ttgggcgtaa agcgagcgca ggcggtttct taagtctgat 540 gtgaaagccc ccggctcaac cggggagggt cattggaaac tgggagactt gagtgcagaa 600 gaggagagtg gaattccatg tgtagcggtg aaatgcgtag atatatggag gaacaccagt 660 ggcgaaggcg gctctctggt ctgtaactga cgctgaggct cgaaagcgtg gggagcaaac 720 aggattagat accctggtag tccacgccgt aaacgatgag tgctaagtgt tggagggttt 780 ccgcccttca gtgctgcagc taacgcatta agcactccgc ctggggagta cgaccgcaag 840 gttgaaactc aaaggaattg acgggggccc gcacaagcgg tggagcatgt ggtttaattc 900 gaagcaacgc gaagaacctt accaggtctt gacatccttt gaccactcta gagatagagc 960 ttccccttcg ggggcaaagt gacaggtggt gcatggttgt cgtcagctcg tgtcgtgaga 1020 tgttgggtta agtcccgcaa cgagcgcaac ccttattgtt agttgccatc atttagttgg 1080 gcactctagc aagactgccg gtgacaaacc gga 1113

<210> 199

<211> 1112

<212> DNA

<213> Enterococcus sp. 325B3_12AGalle

<400> 199 acatgcaagt cgaacgcttc tttttccacc ggagcttgct ccaccggaaa aagaggagtg 60 gcgaacgggt gagtaacacg tgggtaacct gcccatcaga aggggataac acttggaaac 120 aggtgctaat accgtataac aatcgaaacc gcatggtttt gatttgaaag gcgctttcgg 180 gtgtcgctga tggatggacc cgcggtgcat tagctagttg gtgaggtaac ggctcaccaa 240 ggcgacgatg catagccgac ctgagagggt gatcggccac attgggactg agacacggcc 300 caaactccta cgggaggcag cagtagggaa tcttcggcaa tggacgaaag tctgaccgag 360 caacgccgcg tgagtgaaga aggttttcgg atcgtaaaac tctgttgtta gagaagaaca 420 aggatgagag taactgttca tcccttgacg gtatctaacc agaaagccac ggctaactac 480 gtgccagcag ccgcggtaat acgtaggtgg caagcgttgt ccggatttat tgggcgtaaa 540 gcgagcgcag gcggtttctt aagtctgatg tgaaagcccc cggctcaacc ggggagggtc 600 attggaaact gggagacttg agtgcagaag aggagagtgg aattccatgt gtagcggtga 660 aatgcgtaga tatatggagg aacaccagtg gcgaaggcgg ctctctggtc tgtaactgac 720 gctgaggctc gaaagcgtgg ggagcaaaca ggattagata ccctggtagt ccacgccgta 780 aacgatgagt gctaagtgtt ggagggtttc cgcccttcag tgctgcagct aacgcattaa 840 gcactccgcc tggggagtac gaccgcaagg ttgaaactca aaggaattga cgggggcccg 900 cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg aagaacctta ccaggtcttg 960 acatcctttg accactctag agatagagct tccccttcgg gggcaaagtg acaggtggtg 1020 catggttgtc gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc 1080 cttattgtta gttgccatca tttagttggg ca 1112

<210> 200

<211> 1110

<212> DNA

<213> Enterococcus sp. 759B5_12ASB

<400> 200 tgcaagtcga acgctttttc tttcaccgga gcttgctcca ccgaaagaaa aagagtggcg 60 aacgggtgag taacacgtgg gtaacctgcc catcagaagg ggataacact tggaaacagg 120 tgctaatacc gtataacact attttccgca tggaagaaag ttgaaaggcg cttttgcgtc 180 actgatggat ggacccgcgg tgcattagct agttggtgag gtaacggctc accaaggcaa 240 cgatgcatag ccgacctgag agggtgatcg gccacactgg gactgagaca cggcccagac 300 tcctacggga ggcagcagta gggaatcttc ggcaatggac gaaagtctga ccgagcaacg 360 ccgcgtgagt gaagaaggtt ttcggatcgt aaaactctgt tgttagagaa gaacaaggat 420 gagagtaaaa tgttcatccc ttgacggtat ctaaccagaa agccacggct aactacgtgc 480 cagcagccgc ggtaatacgt aggtggcaag cgttgtccgg atttattggg cgtaaagcga 540 gcgcaggcgg tttcttaagt ctgatgtgaa agcccccggc tcaaccgggg agggtcattg 600 gaaactggga gacttgagtg cagaagagga gagtggaatt ccatgtgtag cggtgaaatg 660 cgtagatata tggaggaaca ccagtggcga aggcggctct ctggtctgta actgacgctg 720 aggctcgaaa gcgtggggag cgaacaggat tagataccct ggtagtccac gccgtaaacg 780 atgagtgcta agtgttggag ggtttccgcc cttcagtgct gcagcaaacg cattaagcac 840 tccgcctggg gagtacgacc gcaaggttga aactcaaagg aattgacggg ggcccgcaca 900 agcggtggag catgtggttt aattcgaagc aacgcgaaga accttaccag gtcttgacat 960 cctttgacca ctctagagat agagcttccc cttcgggggc aaagtgacag gtggtgcatg 1020 gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccctta 1080 ttgttagttg ccatcattta agttgggcac 1110

<210> 201

<211> 1105

<212> DNA

<213> Enterococcus sp. 689B1_12EGalle

<400> 201 tcgaacgctt ctttttccac cggagcttgc tccaccggar aaagaggagt ggcgaacggg 60 tgagtaacac gtgggtaacc tgcccatcag aaggggataa cacttggaaa caggtgctaa 120 taccgtataa caatcraaac cgcatggttt tgatttgaaa ggcgctttcg ggtgtcgctg 180 atggatggac ccgcggtgca ttagctagtt ggtgaggtaa cggctcacca aggccacgat 240 gcatagccga cctgagaggg tgatcggcca cattgggact gagacacggc ccaaactcct 300 acgggaggca gcagtaggga atcttcggca atggacgaaa gtctgaccga gcaacgccgc 360 gtgagtgaag aaggttttcg gatcgtaaaa ctctgttgtt agagaagaac aaggatgaga 420 gtaactgttc atcccttgac ggtatctaac cagaaagcca cggctaacta cgtgccagca 480 gccgcggtaa tacgtaggtg gcaagcgttg tccggattta ttgggcgtaa agcgagcgca 540 ggcggtttct taagtctgat gtgaaagccc ccggctcaac cggggagggt cattggaaac 600 tgggagactt gagtgcagaa gaggagagtg gaattccatg tgtagcggtg aaatgcgtag 660 atatatggag gaacaccagt ggcgaaggcg gctctctggt ctgtaactga cgctgaggct 720 cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgccgt aaacgatgag 780 tgctaagtgt tggagggttt ccgcccttca gtgctgcagc taacgcatta agcactccgc 840 ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc gcacaagcgg 900 tggagcatgt ggtttaattc gaagcaacgc gaagaacctt accaggtctt gacatccttt 960 gaccactcta gagatagagc ttccccttcg ggggcaaagt gacaggtggt gcatggttgt 1020 cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac ccttattgtt 1080 agttgccatc attcagttgg gcact 1105

<210> 202

<211> 1103

<212> DNA

<213> Enterococcus sp. 686B1_12ASB

<400> 202 acatgcaagt cgaacgcttt ttctttcacc ggagcttgct ccaccgaaag aaaaagagtg 60 gcgaacgggt gagtaacacg tgggtaacct gcccatcaga aggggataac acttggaaac 120 aggtgctaat accgtataac actattttcc gcatggaaga aagttgaaag gcgcttttgc 180 gtcactgatg gatggacccg cggtgcatta gctagttggt gaggtaacgg ctcaccaagg 240 ccacgatgca tagccgacct gagagggtga tcggccacac tgggactgag acacggccca 300 gactcctacg ggaggcagca gtagggaatc ttcggcaatg gacgaaagtc tgaccgagca 360 acgccgcgtg agtgaagaag gttttcggat cgtaaaactc tgttgttaga gaagaacaag 420 gatgagagta gaacgttcat cccttgacgg tatctaacca gaaagccacg gctaactacg 480 tgccagcagc cgcggtaata cgtaggtggc aagcgttgtc cggatttatt gggcgtaaag 540 cgagcgcagg cggtttctta agtctgatgt gaaagccccc ggctcaaccg gggagggtca 600 ttggaaactg ggagacttga gtgcagaaga ggagagtgga attccatgtg tagcggtgaa 660 atgcgtagat atatggagga acaccagtgg cgaaggcggc tctctggtct gtaactgacg 720 ctgaggctcg aaagcgtggg gagcgaacag gattagatac cctggtagtc cacgccgtaa 780 acgatgagtg ctaagtgttg gagggtttcc gcccttcagt gctgcagcaa acgcattaag 840 cactccgcct ggggagtacg accgcaaggt tgaaactcaa aggaattgac gggggcccgc 900 acaagcggtg gagcatgtgg tttaattcga agcaacgcga agaaccttac caggtcttga 960 catcctttga ccactctaga gatagagctt ccccttcggg ggcaaagtga caggtggtgc 1020 atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg agcgcaaccc 1080 ttattgttag ttgccatcat tta 1103

<210> 203

<211> 1101

<212> DNA

<213> Enterococcus sp. 309B4_12EGalle

<400> 203 tgcaagtcga acgcttcttt ttccaccgga gcttgctcca ccggaaaaag aggagtggcg 60 aacgggtgag taacacgtgg gtaacctgcc catcagaagg ggataacact tggaaacagg 120 tgctaatacc gtataacaat cgaaaccgca tggttttgat ttgaaaggcg ctttcgggtg 180 tcgctgatgg atggacccgc ggtgcattag ctagttggtg aggtaacggc tcaccaaggc 240 gacgatgcat agccgacctg agagggtgat cggccacatt gggactgaga cacggcccaa 300 actcctacgg gaggcagcag tagggaatct tcggcaatgg acgaaagtct gaccgagcaa 360 cgccgcgtga gtgaagaagg ttttcggatc gtaaaactct gttgttagag aagaacaagg 420 atgagagtaa ctgttcatcc cttgacggta tctaaccaga aagccacggc taactacgtg 480 ccagcagccg cggtaatacg taggtggcaa gcgttgtccg gatttattgg gcgtaaagcg 540 agcgcaggcg gtttcttaag tctgatgtga aagcccccgg ctcaaccggg gagggtcatt 600 ggaaactggg agacttgagt gcagaagagg agagtggaat tccatgtgta gcggtgaaat 660 gcgtagatat atggaggaac accagtggcg aaggcggctc tctggtctgt aactgacgct 720 gaggctcgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccgtaaac 780 gatgagtgct aagtgttgga gggtttccgc ccttcagtgc tgcagctaac gcattaagca 840 ctccgcctgg ggagtacgac cgcaaggttg aaactcaaag gaattgacgg gggcccgcac 900 aagcggtgga gcatgtggtt taattcgaag caacgcgaag aaccttacca ggtcttgaca 960 tcctttgacc actctagaga tagagcttcc ccttcggggg caaagtgaca ggtggtgcat 1020 ggttgtcgtc agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag cgcaaccctt 1080 attgttagtt gccatcattt a 1101

<210> 204

<211> 1100

<212> DNA

<213> Enterococcus sp. 630B2_12ASB

<400> 204 acatgcaagt cgtacgcttc tttttccacc ggagcttgct ccaccggaaa aagaagagtg 60 gcgaacgggt gagtaacacg tgggtaacct gcccatcaga aggggataac acttggaaac 120 aggtgctaat accgtataac aatcgaaacc gcatggtttt gatttgaaag gcgctttcgg 180 gtgtcgctga tggatggacc cgcggtgcat tagctagttg gtgaggtaac ggctcaccaa 240 ggccacgatg catagccgac ctgagagggt gatcggccac attgggactg agacacggcc 300 caaactccta cgggaggcag cagtagggaa tcttcggcaa tggacgaaag tctgaccgag 360 caacgccgcg tgagtgaaga aggttttcgg atcgtaaaac tctgttgtta gagaagaaca 420 aggatgagag taactgttca tcccttgacg gtatctaacc agaaagccac ggctaactac 480 gtgccagcag ccgcggtaat acgtaggtgg caagcgttgt ccggatttat tgggcgtaaa 540 gcgagcgcag gcggtttctt aagtctgatg tgaaagcccc cggctcaacc ggggagggtc 600 attggaaact gggagacttg agtgcagaag aggagagtgg aattccatgt gtagcggtga 660 aatgcgtaga tatatggagg aacaccagtg gcgaaggcgg ctctctggtc tgtaactgac 720 gctgaggctc gaaagcgtgg ggagcaaaca ggattagata ccctggtagt ccacgccgta 780 aacgatgagt gctaagtgtt ggagggtttc cgcccttcag tgctgcagct aacgcattaa 840 gcactccgcc tggggagtac gaccgcaagg ttgaaactca aaggaattga cgggggcccg 900 cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg aagaacctta ccaggtcttg 960 acatcctttg accactctag agatagagct tccccttcgg gggcaaagtg acaggtggtg 1020 catggttgtc gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc 1080 cttattgtta gttgccatca 1100

<210> 205

<211> 1087

<212> DNA

<213> Enterococcus sp. 631 B2_12ASB <400> 205 tgcaagtcga acgcttcttt ttccaccgga gcttgctcca ccggaaaaag aggagtggcg 60 aacgggtgag taacacgtgg gtaacctgcc catcagaagg ggataacact tggaaacagg 120 tgctaatacc gtataacaat cgaaaccgca tggttttgat ttgaaaggcg ctttcgggtg 180 tcgctgatgg atggacccgc ggtgcattag ctagttggtg aggtaacggc tcaccaaggc 240 cacgatgcat agccgacctg agagggtgat cggccacatt gggactgaga cacggcccaa 300 actcctacgg gaggcagcag tagggaatct tcggcaatgg acgaaagtct gaccgagcaa 360 cgccgcgtga gtgaagaagg ttttcggatc gtaaaactct gttgttagag aagaacaagg 420 atgagagtaa ctgttcatcc cttgacggta tctaaccaga aagccacggc taactacgtg 480 ccagcagccg cggtaatacg taggtggcaa gcgttgtccg gatttattgg gcgtaaagcg 540 agcgcaggcg gtttcttaag tctgatgtga aagcccccgg ctcaaccggg gagggtcatt 600 ggaaactggg agacttgagt gcagaagagg agagtggaat tccatgtgta gcggtgaaat 660 gcgtagatat atggaggaac accagtggcg aaggcggctc tctggtctgt aactgacgct 720 gaggctcgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccgtaaac 780 gatgagtgct aagtgttgga gggtttccgc ccttcagtgc tgcagctaac gcattaagca 840 ctccgcctgg ggagtacgac cgcaaggttg aaactcaaag gaattgacgg gggcccgcac 900 aagcggtgga gcatgtggtt taattcgaag caacgcgaag aaccttacca ggtcttgaca 960 tcctttgacc actctagaga tagagcttcc ccttcggggg caaagtgaca ggtggtgcat 1020 ggttgtcgtc agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag cgcaaccctt 1080 attgtta 1087

<210> 206

<211> 1399

<212> DNA

<213> bacterium EM-2014-115

<400> 206 ggttacctca ccgacttcgg gtgttacaaa ctctcgtggt gtgacgggcg gtgtgtacaa 60 ggcccgggaa cgtattcacc gcggcgtgct gatccgcgat tactagcgat tccggcttca 120 tgcaggcgag ttgcagcctg caatccgaac tgagagaagc tttaagagat tagcttagcc 180 tcgcgacttc gcaactcgtt gtacttccca ttgtagcacg tgtgtagccc aggtcataag 240 gggcatgatg atttgacgtc atccccacct tcctccggtt tgtcaccggc agtcttgcta 300 gagtgcccaa ctgaatgatg gcaactaaca ataagggttg cgctcgttgc gggacttaac 360 ccaacatctc acgacacgag ctgacgacaa ccatgcacca cctgtcactt tgcccccgaa 420 ggggaagctc tatctctaga gtggtcaaag gatgtcaaga cctggtaagg ttcttcgcgt 480 tgcttcgaat taaaccacat gctccaccgc ttgtgcgggc ccccgtcaat tcctttgagt 540 ttcaaccttg cggtcgtact ccccaggcgg agtgcttaat gcgttagctg cagcactgaa 600 gggcggaaac cctccaacac ttagcactca tcgtttacgg cgtggactac cagggtatct 660 aatcctgttt gctccccacg ctttcgagcc tcagcgtcag ttacagacca gagagccgcc 720 ttcgccactg gtgttcctcc atatatctac gcatttcacc gctacacatg gaattccact 780 ctcctcttct gcactcaagt ctcccagttt ccaatgaccc tccccggttg agccgggggc 840 tttcacatca gacttaagaa accgcctgcg ctcgctttac gcccaataaa tccggacaac 900 gcttgccacc tacgtattac cgcggctgct ggcacgtagt tagccgtggc tttctggtta 960 gataccgtca agggatgaac agttactctc atccttgttc ttctctaaca acagagtttt 1020 acgatccgaa aaccttcttc actcacgcgg cgttgctcgg tcagactttc gtccattgcc 1080 gaagattccc tactgctgcc tcccgtagga gtttgggccg tgtctcagtc ccaatgtggc 1140 cgatcaccct ctcaggtcgg ctatgcatcg tggccttggt gagccgttac ctcaccaact 1200 agctaatgca ccgcgggtcc atccatcagc gacacccgaa agcgcctttc aaatcaaaac 1260 catgcggttt tgattgttat acggtattag cacctgtttc caagtgttat ccccttctga 1320 tgggcaggtt acccacgtgt tactcacccg ttcgccactc ctctttttcc ggtggagcaa 1380 gctccggtgg aaaaagaag 1399

<210> 207

<211> 1321

<212> DNA

<213> Enterococcus hirae 8

<400> 207 tggcggcgtg cctaatacat gcaagtcgaa cgcttctttt tccaccggag cttgctccac 60 cggaaaaaga ggagtggcga acgggtgagt aacacgtggg taacctgccc atcagaaggg 120 gataacactt ggaaacaggt gctaataccg tataacaatc gaaaccgcat ggttttgatt 180 tgaaaggcgc tttcgggtgt cgctgatgga tggacccgcg gtgcattagc tagttggtga 240 ggtaacggct caccaaggcg acgatgcata gccgacctga gagggtgatc ggccacattg 300 ggactgagac acggcccaaa ctcctacggg aggcagcagt agggaatctt cggcaatgga 360 cgaaagtctg accgagcaac gccgcgtgag tgaagaaggt tttcggatcg taaaactctg 420 ttgttagaga agaacaagga tgagagtaac tgttcatccc ttgacggtat ctaaccagaa 480 agccacggct aactacgtgc cagcagccgc ggtaatacgt aggtggcaag cgttgtccgg 540 atttattggg cgtaaagcga gcgcaggcgg tttcttaagt ctgatgtgaa agcccccggc 600 tcaaccgggg agggtcattg gaaactggga gacttgagtg cagaagagga gagtggaatt 660 ccatgtgtag cggtgaaatg cgtagatata tggaggaaca ccagtggcga aggcggctct 720 ctggtctgta actgacgctg aggctcgaaa gcgtggggag caaacaggat tagataccct 780 ggtagtccac gccgtaaacg atgagtgcta agtgttggag ggtttccgcc cttcagtgct 840 gcagctaacg cattaagcac tccgcctggg gagtacgacc gcaaggttga aactcaaagg 900 aattgacggg ggcccgcaca agcggtggag catgtggttt aattcgaagc aacgcgaaga 960 accttaccag gtcttgacat cctttgacca ctctagagat agagcttccc cttcgggggc 1020 aaagtgacag gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc 1080 cgcaacgagc gcaaccctta ttgttagttg ccatcattta gttgggcact ctagcaagac 1140 tgccggtgac aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc 1200 tgggctacac acgtgctaca atgggaagta caacgagtcg caaagtcgcg aggctaagct 1260 aatctcttaa agcttctctc agttcggatt gtaggctgca actcgcctac atgaagccgg 1320 a 1321

<210> 208

<211> 1138

<212> DNA

<213> Enterococcus sp. 930B6_12ESB

<400> 208 acatgcaagt cgtacgcttc tttttccacc ggagcttgct ccaccggaaa aagaagagtg 60 gcgaacgggt gagtaacacg tgggtaacct gcccatcaga aggggataac acttggaaac 120 aggtgctaat accgtataac aatcgaaacc gcatggtttt gatttgaaag gcgctttcgg 180 gtgtcgctga tggatggacc cgcggtgcat tagctagttg gtgaggtaac ggctcaccaa 240 ggccacgatg catagccgac ctgagagggt gatcggccac attgggactg agacacggcc 300 caaactccta cgggaggcag cagtagggaa tcttcggcaa tggacgaaag tctgaccgag 360 caacgccgcg tgagtgaaga aggttttcgg atcgtaaaac tctgttgtta gagaagaaca 420 aggatgagag taactgttca tcccttgacg gtatctaacc agaaagccac ggctaactac 480 gtgccagcag ccgcggtaat acgtaggtgg caagcgttgt ccggatttat tgggcgtaaa 540 gcgagcgcag gcggtttctt aagtctgatg tgaaagcccc cggctcaacc ggggagggtc 600 attggaaact gggagacttg agtgcagaag aggagagtgg aattccatgt gtagcggtga 660 aatgcgtaga tatatggagg aacaccagtg gcgaaggcgg ctctctggtc tgtaactgac 720 gctgaggctc gaaagcgtgg ggagcaaaca ggattagata ccctggtagt ccacgccgta 780 aacgatgagt gctaagtgtt ggagggtttc cgcccttcag tgctgcagct aacgcattaa 840 gcactccgcc tggggagtac gaccgcaagg ttgaaactca aaggaattga cgggggcccg 900 cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg aagaacctta ccaggtcttg 960 acatcctttg accactctag agatagagct tccccttcgg gggcaaagtg acaggtggtg 1020 catggttgtc gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc 1080 cttattgtta gttgccatca ttcagttggg cactctagca agactgccgg tgacaaac 1138

<210> 209

<211> 1487

<212> DNA

<213> Prior Sequence 28

<400> 209 agagtttgaa tcatggctca ggacgaacgc tggcggcgtg cctaatacat gcaagtcgaa 60 cgcttctttt tccaccggag cttgctccac cggaaaaaga ggagtggcga acgggtgagt 120 aacacgtggg taacctgccc atcagaaggg gataacactt ggaaacaggt gctaataccg 180 tataacaatc gaaaccgcat ggttttgatt tgaaaggcgc tttcgggtgt cgctgatgga 240 tggacccgcg gtgcattagc tagttggtga ggtaacggct caccaaggcg acgatgcata 300 gccgacctga gagggtgatc ggccacattg ggactgagac acggcccaaa ctcctacggg 360 aggcagcagt agggaatctt cggcaatgga cgaaagtctg accgagcaac gccgcgtgag 420 tgaagaaggt tttcggatcg taaaactctg ttgttagaga agaacaagga tgagagtaac 480 tgttcatccc ttgacggtat ctaaccagaa agccacggct aactacgtgc cagcagccgc 540 ggtaatacgt aggtggcaag cgttgtccgg atttattggg cgtaaagcga gcgcaggcgg 600 tttcttaagt ctgatgtgaa agcccccggc tcaaccgggg agggtcattg gaaactggga 660 gacttgagtg cagaagagga gagtggaatt ccatgtgtag cggtgaaatg cgtagatata 720 tggaggaaca ccagtggcga aggcggctct ctggtctgta actgacgctg aggctcgaaa 780 gcgtggggag caaacaggat tagataccct ggtagtccac gccgtaaacg atgagtgcta 840 agtgttggag ggtttccgcc cttcagtgct gcagctaacg cattaagcac tccgcctggg 900 gagtacgacc gcaaggttga aactcaaagg aattgacggg ggcccgcaca agcggtggag 960 catgtggttt aattcgaagc aacgcgaaga accttaccag gtcttgacat cctttgacca 1020 ctctagagat agagcttccc cttcgggggc aaagtgacag gtggtgcatg gttgtcgtca 1080 gctcgtgtcg tgagatgttg ggttaagtcc cgcaacgagc gcaaccctta ttgttagttg 1140 ccatcattta gttgggcact ctagcaagac tgccggtgac aaaccggagg aaggtgggga 1200 tgacgtcaaa tcatcatgcc ccttatgacc tgggctacac acgtgctaca atgggaagta 1260 caacgagtcg caaagtcgcg aggctaagct aatctcttaa agcttctctc agttcggatt 1320 gtaggctgca actcgcctac atgaagccgg aatcgctagt aatcgcggat cagcacgccg 1380 cggtgaatac gttcccgggc cttgtacaca ccgcccgtca caccacgaga gtttgtaaca 1440 cccgaagtcg gtgaggtaac cttttggagc cagccgccta aggtgat 1487

<210> 210

<211> 1350 <212> DNA

<213> Prior Sequence 29

<400> 210 ccggaaaaag aggagtggcg aacgggtgag taacacgtgg gtaacctgcc catcagaagg 60 ggataacact tggaaacagg tgctaatacc gtataacaat cgaaaccgca tggttttgat 120 ttgaaaggcg ctttcgggtg tcgctgatgg atggacccgc ggtgcattag ctagttggtg 180 aggtaacggc tcaccaaggc cacgatgcat agccgacctg agagggtgat cggccacatt 240 gggactgaga cacggcccaa actcctacgg gaggcagcag tagggaatct tcggcaatgg 300 acgaaagtct gaccgagcaa cgccgcgtga gtgaagaagg ttttcggatc gtaaaactct 360 gttgttagag aagaacaagg atgagagtaa ctgttcatcc cttgacggta tctaaccaga 420 aagccacggc taactacgtg ccagcagccg cggtaatacg taggtggcaa gcgttgtccg 480 gatttattgg gcgtaaagcg agcgcaggcg gtttcttaag tctgatgtga aagcccccgg 540 ctcaaccggg gagggtcatt ggaaactggg agacttgagt gcagaagagg agagtggaat 600 tccatgtgta gcggtgaaat gcgtagatat atggaggaac accagtggcg aaggcggctc 660 tctggtctgt aactgacgct gaggctcgaa agcgtgggga gcaaacagga ttagataccc 720 tggtagtcca cgccgtaaac gatgagtgct aagtgttgga gggtttccgc ccttcagtgc 780 tgcagctaac gcattaagca ctccgcctgg ggagtacgac cgcaaggttg aaactcaaag 840 gaattgacgg gggcccgcac aagcggtgga gcatgtggtt taattcgaag caacgcaaga 900 accttaccag gtcttgacat cctttgacca ctctagagat agagcttccc cttcgggggc 960 aaagtgacag gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc 1020 cgcaacgagc gcaaccctta ttgttagttg ccatcattca gttgggcact ctagcaagac 1080 tgccggtgac aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc 1140 tgggctacac acgtgctaca atgggaagta caacgagttg cgaagtcgcg aggctaagct 1200 aatctcttaa agcttctctc agttcggatt gcaggctgca actcgcctgc atgaagccgg 1260 aatcgctagt aatcgcggat cagcacgccg cggtgaatac gttcccgggc cttgtacaca 1320 ccgcccgtca caccacgaga gtgtatcaca 1350

<210> 211

<211> 1436

<212> DNA

<213> Prior Sequence 30

<400> 211 catgcaagtc gaacgcttct ttttccaccg gagcttgctc caccggaaaa agaggagtgg 60 cgaacgggtg agtaacacgt gggtaacctg cccatcagaa ggggataaca cttggaaaca 120 ggtgctaata ccgtataaca atcgaaaccg catggttttg atttgaaagg cgctttcggg 180 tgtcgctgat ggatggaccc gcggtgcatt agctagttgg tgaggtaacg gctcaccaag 240 gcgacgatgc atagccgacc tgagagggtg atcggccaca ttgggactga gacacggccc 300 aaactcctac gggaggcagc agtagggaat cttcggcaat ggacgaaagt ctgaccgagc 360 aacgccgcgt gagtgaagaa ggttttcgga tcgtaaaact ctgttgttag agaagaacaa 420 ggatgagagt aactgttcat cccttgacgg tatctaacca gaaagccacg gctaactacg 480 tgccagcagc cgcggtaata cgtaggtggc aagcgttgtc cggatttatt gggcgtaaag 540 cgagcgcagg cggtttctta agtctgatgt gaaagccccc ggctcaaccg gggagggtca 600 ttggaaactg ggagacttga gtgcagaaga ggagagtgga attccatgtg tagcggtgaa 660 atgcgtagat atatggagga acaccagtgg cgaaggcggc tctctggtct gtaactgacg 720 ctgaggctcg aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgccgtaa 780 acgatgagtg ctaagtgttg gagggtttcc gcccttcagt gctgcagcta acgcattaag 840 cactccgcct ggggagtacg accgcaaggt tgaaactcaa aggaattgac gggggcccgc 900 acaagcggtg gagcatgtgg tttaattcga agcaacgcga agaaccttac caggtcttga 960 catcctttga ccactctaga gatagagctt ccccttcggg ggcaaagtga caggtggtgc 1020 atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg agcgcaaccc 1080 ttattgttag ttgccatcat ttagttgggc actctagcaa gactgccggt gacaaaccgg 1140 aggaaggtgg ggatgacgtc aaatcatcat gccccttatg acctgggcta cacacgtgct 1200 acaatgggaa gtacaacgag tcgcaaagtc gcgaggctaa gctaatctct taaagcttct 1260 ctcagttcgg attgtaggct gcaactcgcc tacatgaagc cggaatcgct agtaatcgcg 1320 gatcagcacg ccgcggtgaa tacgttcccg ggccttgtac acaccgcccg tcacaccacg 1380 agagtttgta acacccgaag tcggtgaggt aaccttttgg agccagccgc ctaagg 1436

<210> 212

<211> 1475

<212> DNA

<213> Prior Sequence 31

<400> 212 acgacgctgg cggcgtgcct atacatgcaa gtcgaacgct tctttttcca ccggagcttg 60 ctccaccgga aaaagaggag tggcgaacgg gtgagtaaca cgtgggtaac ctgcccatca 120 gaaggggata acacttggaa acaggtgcta ataccgtata acaatcgaaa ccgcatggtt 180 ttgatttgaa aggcgctttc gggtgtcgct gatggatgga cccgcggtgc attagctagt 240 tggtgaggta acggctcacc aaggcgacga tgcatagccg acctgagagg gtgatcggcc 300 acattgggac tgagacacgg cccaaactcc tacgggaggc agcagtaggg aatcttcggc 360 aatggacgaa agtctgaccg agcaacgccg cgtgagtgaa gaaggttttc ggatcgtaaa 420 actctgttgt tagagaagaa caaggatgag agtaactgtt catcccttga cggtatctaa 480 ccagaaagcc acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt 540 gtccggattt attgggcgta aagcgagcgc aggcggtttc ttaagtctga tgtgaaagcc 600 cccggctcaa ccggggaggg tcattggaaa ctgggagact tgagtgcaga agaggagagt 660 ggaattccat gtgtagcggt gaaatgcgta gatatatgga ggaacaccag tggcgaaggc 720 ggctctctgg tctgtaactg acgctgaggc tcgaaagcgt ggggagcaaa caggattaga 780 taccctggta gtccacgccg taaacgatga gtgctaagtg ttggagggtt tccgcccttc 840 agtgctgcag ctaacgcatt aagcactccg cctggggagt acgaccgcaa ggttgaaact 900 caaaggaatt gacgggggcc cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg 960 cgaagaacct taccaggtct tgacatcctt tgaccactct agagatagag cttccccttc 1020 gggggcaaag tgacaggtgg tgcatggttg tcgtcagctc gtgtcgtgag atgttgggtt 1080 aagtcccgca acgagcgcaa cccttattgt tagttgccat catttagttg ggcactctag 1140 caagactgcc ggtgacaaac cggaggaagg tggggatgac gtcaaatcat catgcccctt 1200 atgacctggg ctacacacgt gctacaatgg gaagtacaac gagtcgcaaa gtcgcgaggc 1260 taagctaatc tcttaaagct tctctcagtt cggattgtag gctgcaactc gcctacatga 1320 agccggaatc gctagtaatc gcggatcagc acgccgcggt gaatacgttc ccgggccttg 1380 tacacaccgc ccgtcacacc acgagagttt gtaacacccg aagtcggtga ggtaaccttt 1440 tggagccagc cgcctaaggt ggatagagac tggag 1475

<210> 213

<211> 1467

<212> DNA

<213> Prior Sequence 32

<400> 213 ctggcggcgt gctatacatg caagtcgaac gcttcttttt ccaccggagc ttgctccacc 60 ggaaaaagag gagtggcgaa cgggtgagta acacgtgggt aacctgccca tcagaagggg 120 ataacacttg gaaacaggtg ctaataccgt ataacaatcg aaaccgcatg gttttgattt 180 gaaaggcgct ttcgggtgtc gctgatggat ggacccgcgg tgcattagct agttggtgag 240 gtaacggctc accaaggcga cgatgcatag ccgacctgag agggtgatcg gccacattgg 300 gactgagaca cggcccaaac tcctacggga ggcagcagta gggaatcttc ggcaatggac 360 gaaagtctga ccgagcaacg ccgcgtgagt gaagaaggtt ttcggatcgt aaaactctgt 420 tgttagagaa gaacaaggat gagagtaact gttcatccct tgacggtatc taaccagaaa 480 gccacggcta actacgtgcc agcagccgcg gtaatacgta ggtggcaagc gttgtccgga 540 tttattgggc gtaaagcgag cgcaggcggt ttcttaagtc tgatgtgaaa gcccccggct 600 caaccgggga gggtcattgg aaactgggag acttgagtgc agaagaggag agtggaattc 660 catgtgtagc ggtgaaatgc gtagatatat ggaggaacac cagtggcgaa ggcggctctc 720 tggtctgtaa ctgacgctga ggctcgaaag cgtggggagc aaacaggatt agataccctg 780 gtagtccacg ccgtaaacga tgagtgctaa gtgttggagg gtttccgccc ttcagtgctg 840 cagctaacgc attaagcact ccgcctgggg agtacgaccg caaggttgaa actcaaagga 900 attgacgggg gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgaagaa 960 ccttaccagg tcttgacatc ctttgaccac tctagagata gagcttcccc ttcgggggca 1020 aagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc 1080 gcaacgagcg caacccttat tgttagttgc catcatttag ttgggcactc tagcaagact 1140 gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct 1200 gggctacaca cgtgctacaa tgggaagtac aacgagtcgc aaagtcgcga ggctaagcta 1260 atctcttaaa gcttctctca gttcggattg taggctgcaa ctcgcctaca tgaagccgga 1320 atcgctagta atcgcggatc agcacgccgc ggtgaatacg ttcccgggcc ttgtacacac 1380 cgcccgtcac accacgagag tttgtaacac ccgaagtcgg tgaggtaacc ttttggagcc 1440 agccgcctaa ggtggataga gactttg 1467

<210> 214

<211> 1478

<212> DNA

<213> Prior Sequence 33

<400> 214 ggagacgctg gcggcgtgcc taatacatgc aagtcgaacg cttctttttc caccggagct 60 tgctccaccg gaaaaagagg agtggcgaac gggtgagtaa cacgtgggta acctgcccat 120 cagaagggga taacacttgg aaacaggtgc taataccgta taacaatcga aaccgcatgg 180 ttttgatttg aaaggcgctt tcgggtgtcg ctgatggatg gacccgcggt gcattagcta 240 gttggtgagg taacggctca ccaaggcgac gatgcatagc cgacctgaga gggtgatcgg 300 ccacattggg actgagacac ggcccaaact cctacgggag gcagcagtag ggaatcttcg 360 gcaatggacg aaagtctgac cgagcaacgc cgcgtgagtg aagaaggttt tcggatcgta 420 aaactctgtt gttagagaag aacaaggatg agagtaactg ttcatccctt gacggtatct 480 aaccagaaag ccacggctaa ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg 540 ttgtccggat ttattgggcg taaagcgagc gcaggcggtt tcttaagtct gatgtgaaag 600 cccccggctc aaccggggag ggtcattgga aactgggaga cttgagtgca gaagaggaga 660 gtggaattcc atgtgtagcg gtgaaatgcg tagatatatg gaggaacacc agtggcgaag 720 gcggctctct ggtctgtaac tgacgctgag gctcgaaagc gtggggagca aacaggatta 780 gataccctgg tagtccacgc cgtaaacgat gagtgctaag tgttggaggg tttccgccct 840 tcagtgctgc agctaacgca ttaagcactc cgcctgggga gtacgaccgc aaggttgaaa 900 ctcaaaggaa ttgacggggg cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa 960 cgcgaagaac cttaccaggt cttgacatcc tttgaccact ctagagatag agcttcccct 1020 tcgggggcaa agtgacaggt ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg 1080 ttaagtcccg caacgagcgc aacccttatt gttagttgcc atcatttagt tgggcactct 1140 agcaagactg ccggtgacaa accggaggaa ggtggggatg acgtcaaatc atcatgcccc 1200 ttatgacctg ggctacacac gtgctacaat gggaagtaca acgagtcgca aagtcgcgag 1260 gctaagctaa tctcttaaag cttctctcag ttcggattgt aggctgcaac tcgcctacat 1320 gaagccggaa tcgctagtaa tcgcggatca gcacgccgcg gtgaatacgt tcccgggcct 1380 tgtacacacc gcccgtcaca ccacgagagt ttgtaacacc cgaagtcggt gaggtaacct 1440 tttggagcca gccgcctaag gtggatagag attggggg 1478

<210> 215

<211> 1469

<212> DNA

<213> Prior Sequence 34

<400> 215 ctggcggcgt gcgtatacat gcaagtcgaa cgcttctttt tccaccggag cttgctccac 60 cggaaaaaga ggagtggcga acgggtgagt aacacgtggg taacctgccc atcagaaggg 120 gataacactt ggaaacaggt gctaataccg tataacaatc gaaaccgcat ggttttgatt 180 tgaaaggcgc tttcgggtgt cgctgatgga tggacccgcg gtgcattagc tagttggtga 240 ggtaacggct caccaaggcg acgatgcata gccgacctga gagggtgatc ggccacattg 300 ggactgagac acggcccaaa ctcctacggg aggcagcagt agggaatctt cggcaatgga 360 cgaaagtctg accgagcaac gccgcgtgag tgaagaaggt tttcggatcg taaaactctg 420 ttgttagaga agaacaagga tgagagtaac tgttcatccc ttgacggtat ctaaccagaa 480 agccacggct aactacgtgc cagcagccgc ggtaatacgt aggtggcaag cgttgtccgg 540 atttattggg cgtaaagcga gcgcaggcgg tttcttaagt ctgatgtgaa agcccccggc 600 tcaaccgggg agggtcattg gaaactggga gacttgagtg cagaagagga gagtggaatt 660 ccatgtgtag cggtgaaatg cgtagatata tggaggaaca ccagtggcga aggcggctct 720 ctggtctgta actgacgctg aggctcgaaa gcgtggggag caaacaggat tagataccct 780 ggtagtccac gccgtaaacg atgagtgcta agtgttggag ggtttccgcc cttcagtgct 840 gcagctaacg cattaagcac tccgcctggg gagtacgacc gcaaggttga aactcaaagg 900 aattgacggg ggcccgcaca agcggtggag catgtggttt aattcgaagc aacgcgaaga 960 accttaccag gtcttgacat cctttgacca ctctagagat agagcttccc cttcgggggc 1020 aaagtgacag gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc 1080 cgcaacgagc gcaaccctta ttgttagttg ccatcattta gttgggcact ctagcaagac 1140 tgccggtgac aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc 1200 tgggctacac acgtgctaca atgggaagta caacgagtcg caaagtcgcg aggctaagct 1260 aatctcttaa agcttctctc agttcggatt gtaggctgca actcgcctac atgaagccgg 1320 aatcgctagt aatcgcggat cagcacgccg cggtgaatac gttcccgggc cttgtacaca 1380 ccgcccgtca caccacgaga gtttgtaaca cccgaagtcg gtgaggtaac cttttggagc 1440 cagccgccta aggtggatag agactgggg 1469

<210> 216

<211> 1468

<212> DNA

<213> Prior Sequence 35

<400> 216 ctggcggcgt gctaatacat gcaagtcgaa cgcttctttt tccaccggag cttgctccac 60 cggaaaaaga ggagtggcga acgggtgagt aacacgtggg taacctgccc atcagaaggg 120 gataacactt ggaaacaggt gctaataccg tataacaatc gaaaccgcat ggttttgatt 180 tgaaaggcgc tttcgggtgt cgctgatgga tggacccgcg gtgcattagc tagttggtga 240 ggtaacggct caccaaggcg acgatgcata gccgacctga gagggtgatc ggccacattg 300 ggactgagac acggcccaaa ctcctacggg aggcagcagt agggaatctt cggcaatgga 360 cgaaagtctg accgagcaac gccgcgtgag tgaagaaggt tttcggatcg taaaactctg 420 ttgttagaga agaacaagga tgagagtaac tgttcatccc ttgacggtat ctaaccagaa 480 agccacggct aactacgtgc cagcagccgc ggtaatacgt aggtggcaag cgttgtccgg 540 atttattggg cgtaaagcga gcgcaggcgg tttcttaagt ctgatgtgaa agcccccggc 600 tcaaccgggg agggtcattg gaaactggga gacttgagtg cagaagagga gagtggaatt 660 ccatgtgtag cggtgaaatg cgtagatata tggaggaaca ccagtggcga aggcggctct 720 ctggtctgta actgacgctg aggctcgaaa gcgtggggag caaacaggat tagataccct 780 ggtagtccac gccgtaaacg atgagtgcta agtgttggag ggtttccgcc cttcagtgct 840 gcagctaacg cattaagcac tccgcctggg gagtacgacc gcaaggttga aactcaaagg 900 aattgacggg ggcccgcaca agcggtggag catgtggttt aattcgaagc aacgcgaaga 960 accttaccag gtcttgacat cctttgacca ctctagagat agagcttccc cttcgggggc 1020 aaagtgacag gtggtgcatg gttgtcgtca gctcgtgtcg tgagatgttg ggttaagtcc 1080 cgcaacgagc gcaaccctta ttgttagttg ccatcattta gttgggcact ctagcaagac 1140 tgccggtgac aaaccggagg aaggtgggga tgacgtcaaa tcatcatgcc ccttatgacc 1200 tgggctacac acgtgctaca atgggaagta caacgagtcg caaagtcgcg aggctaagct 1260 aatctcttaa agcttctctc agttcggatt gtaggctgca actcgcctac atgaagccgg 1320 aatcgctagt aatcgcggat cagcacgccg cggtgaatac gttcccgggc cttgtacaca 1380 ccgcccgtca caccacgaga gtttgtaaca cccgaagtcg gtgaggtaac cttttggagc 1440 cagccgccta aggtggatag agactggt 1468

<210> 217

<211> 1477

<212> DNA

<213> Prior Sequence 36

<400> 217 gggagacgct ggcggcgtgc ctatacatgc aagtcgaacg cttctttttc caccggagct 60 tgctccaccg gaaaaagagg agtggcgaac gggtgagtaa cacgtgggta acctgcccat 120 cagaagggga taacacttgg aaacaggtgc taataccgta taacaatcga aaccgcatgg 180 ttttgatttg aaaggcgctt tcgggtgtcg ctgatggatg gacccgcggt gcattagcta 240 gttggtgagg taacggctca ccaaggcgac gatgcatagc cgacctgaga gggtgatcgg 300 ccacattggg actgagacac ggcccaaact cctacgggag gcagcagtag ggaatcttcg 360 gcaatggacg aaagtctgac cgagcaacgc cgcgtgagtg aagaaggttt tcggatcgta 420 aaactctgtt gttagagaag aacaaggatg agagtaactg ttcatccctt gacggtatct 480 aaccagaaag ccacggctaa ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg 540 ttgtccggat ttattgggcg taaagcgagc gcaggcggtt tcttaagtct gatgtgaaag 600 cccccggctc aaccggggag ggtcattgga aactgggaga cttgagtgca gaagaggaga 660 gtggaattcc atgtgtagcg gtgaaatgcg tagatatatg gaggaacacc agtggcgaag 720 gcggctctct ggtctgtaac tgacgctgag gctcgaaagc gtggggagca aacaggatta 780 gataccctgg tagtccacgc cgtaaacgat gagtgctaag tgttggaggg tttccgccct 840 tcagtgctgc agctaacgca ttaagcactc cgcctgggga gtacgaccgc aaggttgaaa 900 ctcaaaggaa ttgacggggg cccgcacaag cggtggagca tgtggtttaa ttcgaagcaa 960 cgcgaagaac cttaccaggt cttgacatcc tttgaccact ctagagatag agcttcccct 1020 tcgggggcaa agtgacaggt ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg 1080 ttaagtcccg caacgagcgc aacccttatt gttagttgcc atcatttagt tgggcactct 1140 agcaagactg ccggtgacaa accggaggaa ggtggggatg acgtcaaatc atcatgcccc 1200 ttatgacctg ggctacacac gtgctacaat gggaagtaca acgagtcgca aagtcgcgag 1260 gctaagctaa tctcttaaag cttctctcag ttcggattgt aggctgcaac tcgcctacat 1320 gaagccggaa tcgctagtaa tcgcggatca gcacgccgcg gtgaatacgt tcccgggcct 1380 tgtacacacc gcccgtcaca ccacgagagt ttgtaacacc cgaagtcggt gaggtaacct 1440 tttggagcca gccgcctaag gtggatagag actgggg 1477