Title:
BISPECIFIC CHIMERIC ANTIGEN RECEPTOR TARGETING HIV-1 ENVELOPE PROTEIN, PREPARATION METHOD THEREFOR AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/077943
Kind Code:
A1
Abstract:
Provided are a bispecific chimeric antigen receptor targeting an HIV-1 envelope protein. The chimeric antigen receptor comprises: (1) a recognition unit targeting an HIV-1gp120 protein co-receptor binding site; (2) a recognition unit targeting another HIV-1 gp120 binding site (including, but not limited to, a CD4 binding site, a V1V2 glycan region, a V3 glycan region, a gp120-gp41 interface, or a membrane proximal external region on gp41); (3) hinge and transmembrane regions; and (4) an intracellular signaling domain; optionally, the chimeric antigen receptor further comprises (5) one or more co-stimulatory signal domains; preferably, the extracellular recognition domains of the chimeric antigen receptor that are sequentially connected in series is the recognition unit targeting the HIV-1gp120 protein co-receptor binding site at the end away from a membrane, and the recognition unit targeting the another HIV-1 gp120 binding site at the end close to the membrane. Provided is a new construction method for a bispecific chimeric antigen receptor targeting an HIV-1 envelope protein, the immune cells modified by the bispecific chimeric antigen receptor have stronger activation and killing ability, and can specifically recognize and kill HIV-1 infected cells.
Inventors:
XU JIANQING (CN)
ZHANG XIAOYAN (CN)
YING TIANLEI (CN)
MAO YUNYU (CN)
LIAO QIBIN (CN)
ZHANG XIAOYAN (CN)
YING TIANLEI (CN)
MAO YUNYU (CN)
LIAO QIBIN (CN)
Application Number:
PCT/CN2022/117012
Publication Date:
May 11, 2023
Filing Date:
September 05, 2022
Export Citation:
Assignee:
UNIV FUDAN (CN)
International Classes:
C07K19/00; A61K39/00; A61P31/18; C12N5/10; C12N15/62; C12N15/867
Foreign References:
| CN111954677A | 2020-11-17 | |||
| CN114163534A | 2022-03-11 | |||
| US20130108636A1 | 2013-05-02 | |||
| CN111196858A | 2020-05-26 | |||
| CN112368052A | 2021-02-12 |
Other References:
OLLERTON MATTHEW T., BERGER EDWARD A., CONNICK ELIZABETH, BURTON GREGORY F.: "HIV-1-Specific Chimeric Antigen Receptor T Cells Fail To Recognize and Eliminate the Follicular Dendritic Cell HIV Reservoir In Vitro", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 94, no. 10, 4 May 2020 (2020-05-04), US , XP093063257, ISSN: 0022-538X, DOI: 10.1128/JVI.00190-20
Attorney, Agent or Firm:
JW IP LAW FIRM (CN)
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