BLOCKING COMPOSITIONS FIELD OF THE INVENTION The present invention relates to a substrate (e.g. a sponge) comprising a blocking composition. The invention has particular, but not necessarily exclusive, application in blocking a cleaning composition to facilitate detection of microorganisms (such as bacteria) on a surface. BACKGROUND Microorganisms, such as bacteria, typically require metal species (e.g. iron), for a number of biological processes. Bacteria may extract iron from the ambient environment by means of iron- chelating compounds known as siderophores (e.g. enterobactin and deferoxamine). Siderophores secreted by bacteria have high iron (Fe ³⁺ ) binding affinity and are thereby able to sequester ambient iron. After sequestration, the iron bound to the siderophore can be taken up by the bacteria using active transport mechanisms. Chromeazurol S (CAS) is a colour changing agent which provides a change in colour upon binding and/or release of iron (blue or purple on binding, orange on release/in unbound form). Due to a high iron binding affinity, when CAS with bound iron is exposed to siderophores, the iron is released from the CAS and instead binds preferentially to the siderophores, thereby resulting in a colour change in the CAS. Levels of bacteria present in a sample are related to the amount of siderophore present in that sample, and the amount of siderophore present is related to the amount of iron that can be sequestered. Similarly, the colour change resulting from binding and/or release of iron is related to the amount of iron bound and/or released. Therefore, the colour change resulting from release of iron bound to CAS may be used to determine the presence or absence of bacteria present in a sample and various colour changing compositions have been produced which rely on these principles. Other microorganisms adopt similar techniques for iron uptake, and other metals can be sequestered in the same manner. Similarly, other colour changing agents can be used to monitor this. In many environments, it is desirable to reduce or eliminate microorganisms, and thereby to reduce or eliminate risk of infection to living organisms. Suitably, this is undertaken using detergent compositions, disinfecting compositions, or the like. Such compositions operate by virtue of a variety of chemical species, such as surfactants, chelators, pH-altering agents, etc.. However, these chemical species can undesirably interact (i.e. interfere) with colour changing agents/compositions configured to detect microorganisms. For example, chelators can themselves chelate iron and prevent uptake by CAS and siderophores. This affects various properties of the colour changing compositions, such as sensitivity, selectivity, stability, etc. and may ultimately lead to an ineffective colour changing composition (e.g. eliciting false positives or false negatives). It is desirable to provide an alternative and/or improved technique for detecting microorganisms, and/or otherwise to obviate and/or mitigate one or more of the disadvantages with known techniques, whether identified herein or otherwise. SUMMARY According to a first aspect, provided herein is a substrate (optionally a sponge) comprising a blocking composition, wherein said blocking composition comprises: one or more metals; one or more blocking composition surfactants; and one or more pH buffers; wherein 0.15 ml of said composition comprises a combined amount of said metal and said blocking composition surfactant that is sufficient to block: at least around 1.372 x 10 ^-8 moles of n-alkyl dimethyl benzyl ammonium chloride; and at least around 3.106 x 10 ^-8 moles of C ₉ -C ₁₁ alkyl alcohol ethoxylate. According to a second aspect, provided herein is a substrate (optionally a sponge) comprising a blocking composition (optionally around 0.15 ml thereof), wherein said blocking composition comprises: one or more metals; one or more blocking composition surfactants; and one or more pH buffers, optionally wherein around 0.15 ml of the blocking composition comprises a combined amount of said metal and said blocking composition surfactant that is sufficient to block: (a) at least around 1.937 x 10 ^-8 moles of citrate, optionally at least around 4.029x10- ⁷ , optionally at least around 7.864x10 ^-7 , optionally at least around 1.17x10 ^-6 , optionally at least around 1.553x10 ^-6 , optionally around 1.9x10 ^-6 such as around 1.937x10 ^-6 ; and/or (b) at least around 2.959 x 10 ^-8 moles of ethylenediaminetetraacetic acid (EDTA), optionally at least around 6.155x10 ^-7 , optionally at least around 1.201x10 ^-6 , optionally at least around 1.787x10 ^-6 , optionally at least around 2.373x10 ^-6 , optionally around 3x10 ^-6 such as around 2.959x10 ^-6 ; and/or (c) at most around 1.937 x 10 ^-8 moles of citrate, optionally at most around 1.553x10- ⁴ , optionally at most around 1.17x10 ^-4 , optionally at most around 7.864x10 ^-5 , optionally at most around 4.029x10 ^-5 , optionally around 1.9x10 ^-6 such as around 1.937x10 ^-6 ; and/or (d) at most around 1.937 x 10 ^-4 moles of ethylenediaminetetraacetic acid (EDTA), optionally at most around 2.373x10 ^-4 , optionally at most around 1.787x10 ^-4 , optionally at most around 1.201x10 ^-4 , optionally at most around 6.155x10 ^-5 , optionally around 3x10 ^-6 such as around 2.959x10 ^-6 ; and/or (e) around 1.937 x 10 ^-8 to 1.937 x 10 ^-4 moles of citrate, optionally around 4.029x10 ^-7 to 1.553x10 ^-4 , optionally around 7.864x10 ^-7 to 1.17x10 ^-4 , optionally around 1.17x10 ^-6 to 7.864x10 ^-5 , optionally around 1.553x10 ^-6 to 4.029x10 ^-5 ; and/or (f) around 2.959 x 10 ^-8 to 1.937 x 10 ^-4 moles of ethylenediaminetetraacetic acid (EDTA), optionally around 6.155x10 ^-7 to 2.373x10 ^-4 , optionally around 1.201x10 ^-6 to 1.787x10 ^-4 , optionally around 1.787x10 ^-6 to 1.201x10 ^-4 , optionally around 2.373x10 ^-6 to 6.155x10 ^-5 . According to a third aspect, provided herein is a composition comprising the blocking composition as defined in the first or second aspect. According to a fourth aspect, provided herein is a kit comprising: the substrate according to the first or second aspect and/or the composition according to the third aspect; and (a) a colour changing composition configured to detect the presence of microorganisms; and/or (b) a cleaning composition configured to reduce and/or eliminate microorganisms. According to a fifth aspect, provided herein is a swab comprising a rod having a substrate according to the first or second aspect at an end thereof (optionally wherein the substrate comprises 0.15 ml of said composition), optionally further comprising a reservoir of a colour changing composition. According to a sixth aspect, provided herein is a use of a substrate according to the first or second aspect, the composition according to third aspect or a swab according to the fifth aspect to block a cleaning composition. According to a seventh aspect, provided herein is a method, comprising contacting a surface, or a sample therefrom, that has been pre-treated with a cleaning composition, with the composition of the third aspect. DEFINITIONS The following definitions apply for terms used herein. In the event that any term is not specifically defined here or otherwise, the standard meaning in the present technical field prevails. This standard meaning may bear in mind definitions provided in common general knowledge (e.g. standard textbooks) in the present technical field. Usefully, for example, chemical terms may be interpreted in accordance with the IUPAC Gold Book Version 3.0.1. As used herein the term “about” or “around” generally encompasses or refers to a range of values that one skilled in the art would consider equivalent to the recited values (i.e. having the same function or result). Where the term “about” or “around” is used in relation to a numerical value or range, it can represent (in increasing order of preference) a 10%, 5%, 2%, 1% or 0% deviation from that value/range. As used herein, the term “substantially” is intended to modify a quality such that a given feature need not be “exactly” in accordance with that quality. Suitably, this modifier may indicate a deviation from the quality given of less than or equal to about 20%, such as less than or equal to about 15%, such as less than or equal to about 10%, such as less than or equal to about 5%, such as less than or equal to about 1%, such as about 0%. Generally speaking, lower deviation is preferred. The term "aliphatic", as used herein, means a straight-chain, branched or cyclic hydrocarbon, which is completely saturated, or which contains one or more units of unsaturation (e.g. alkenyl or alkynyl), but which is not aromatic. Where the aliphatic group refers to a range, such as C ₁ to C ₁₀ , it is to be understood that it includes each member of the range, i.e. C ₁ , C ₂ , C ₃ , C ₄ , C ₅ , C ₆ , etc. The term “(C _1-6 )aliphatic” or “C ₁ to C ₆ ” aliphatic as used herein means an aliphatic group having 1 to 6 carbon atoms, which may be branched or unbranched, saturated or unsaturated and optionally contains a ring. The term “alkyl” refers to a saturated (no double or triple bonds) aliphatic hydrocarbon radical, including straight-chain, and, where possible, branched-chain and cyclic groups and hybrids thereof, such as (cycloalkyl)alkyl. Where the alkyl group refers to a range, such as C ₁ to C ₁₀ , it is to be understood that it includes each member of the range, i.e. C ₁ , C ₂ , C ₃ , C ₄ , C ₅ , C ₆ , etc. The term “(C1-6)alkyl” as used herein means an alkyl group having 1-6 carbon atoms, which may be branched or unbranched and optionally contains a ring. The term “cycloalkyl” refers to a cyclic alkyl group, for example cycloheptyl, cyclohexyl, cyclopentyl, cyclobutyl or cyclopropyl. Cycloalkyl may be substituted as defined herein. As used herein, “alkenyl” refers to an alkyl group that contains in the straight or branched hydrocarbon chain one or more double bonds. Examples of alkenyl groups include allenyl, vinylmethyl and ethenyl. An alkenyl group may be unsubstituted or substituted. As used herein, “alkynyl” refers to an alkyl group that contains in the straight or branched hydrocarbon chain one or more triple bonds. Examples of alkynyls include ethynyl and propynyl. An alkynyl group may be unsubstituted or substituted. The term “alkanoate” means -C(O)O-alkyl wherein alkyl has the meaning as defined above (e.g. being C ₁ to C ₁₀ ). Examples of (C ₁ - ₄ )alkanoate include methanoate, ethanoate, propanoate, isopropanoate, butanoate, isobutanoate and tertiary butanoate. The term “halide” or “halogen” are used interchangeably and, as used herein mean an ion derived from IUPAC group number 17 of the periodic table. The halide/halogen may be a fluoride, a chloride, a bromide, an iodide and the like. As used herein, “aryl” refers to a carbocyclic (all carbon) monocyclic or multicyclic aromatic ring system (including fused ring systems where two carbocyclic rings share a chemical bond) that has a fully delocalized pi-electron system throughout all the rings. The number of carbon atoms in an aryl group can vary. For example, the aryl group can be a C ₆ -C ₁₄ aryl group, a C ₆ -C ₁₀ aryl group, or a C ₆ aryl group. Examples of aryl groups include, but are not limited to, benzene, naphthalene and azulene. As used herein, “heteroaryl” refers to a monocyclic or multicyclic aromatic ring system (a ring system with fully delocalized pi-electron system) that contain(s) one or more heteroatoms (for example, 1 to 5 heteroatoms), that is, an element other than carbon, including but not limited to, nitrogen, oxygen and sulfur. The number of atoms in the ring(s) of a heteroaryl group can vary. For example, the heteroaryl group can contain 4 to 14 atoms in the ring(s), 5 to 10 atoms in the ring(s) or 5 to 6 atoms in the ring(s). Furthermore, the term “heteroaryl” includes fused ring systems where two rings, such as at least one aryl ring and at least one heteroaryl ring, or at least two heteroaryl rings, share at least one chemical bond. Examples of heteroaryl rings include, but are not limited to, furan, furazan, thiophene, benzothiophene, phthalazine, pyrrole, oxazole, benzoxazole, 1,2,3-oxadiazole, 1,2,4-oxadiazole, thiazole, 1,2,3-thiadiazole, 1,2,4-thiadiazole, benzothiazole, imidazole, benzimidazole, indole, indazole, pyrazole, benzopyrazole, isoxazole, benzoisoxazole, isothiazole, triazole, benzotriazole, thiadiazole, tetrazole, pyridine, pyridazine, pyrimidine, pyrazine, purine, pteridine, quinoline, isoquinoline, quinazoline, quinoxaline, cinnoline and triazine. Ethylenediaminetetraacetic acid, referred to as “EDTA”, can be present as a salt, such as a tetrasalt (e.g. tetrasodium) thereof. As used herein, the phrase “colour changing composition” is intended to refer to a composition that comprises an agent (e.g. a dye) configured to change colour upon detection of microbes. The colour changing composition may comprise a dye and a metal, wherein the metal is bindable to the colour changing agent to provide a change in colour on binding and/or release thereof. Such colour changing compositions can be used to detect the presence of microorganisms by means of a colour change arising from an interaction between the colour changing agent, metal and siderophores as released by microorganisms (e.g. bacteria). For example, siderophores can sequester said metal, releasing this from the colour changing agent and thereby causing said colour change. As used herein, the phrase “colour changing complex” refers to a complex formed between the metal and the colour changing agent. Suitable colour changing compositions are described in international patent application WO2018185486, the entire content of which is hereby incorporated in its entirety. The colour changing composition defined herein may be a sprayable composition in accordance with the first aspect defined in WO2018185486, and related implementations thereof. The colour changing composition defined herein may be a composition in accordance with the second aspect defined in WO2018185486, and related implementations thereof. As used herein, the term “metal” is intended to include elemental forms or compounds of metals. As used herein, the phrase “cleaning composition” as used herein is intended to refer to an aqueous composition comprising one or more cleaning chemicals, such as surfactants, emulsifiers, chelators, etc. The cleaning composition may be a “detergent composition” and/or a “disinfectant composition”. As used herein, the phrase “blocking composition” refers to a composition which is able to substantially prevent and/or mitigate the ability of cleaning chemicals in a cleaning composition to interfere with activity of a colour changing composition as discussed herein (in particular a metal-dye based colour changing composition, such as an iron-CAS based composition). Such cleaning chemicals may be left as residue on a surface after cleaning with a cleaning composition. Use of a colour changing composition on a residue-contaminated surface may not elicit the correct colour change in view of unwanted interactions of the residue with the colour changing composition. For example, chelators present in the cleaning composition may interact with metal/iron in a metal-dye based colour changing composition and thereby interfere with the colour change otherwise initiated by binding and release of said metal. Use of the blocking composition discussed herein on the residue substantially prevents and/or mitigates interference such as this, so that the surface is ready for the detection of microorganisms by the colour changing composition without or substantially without interference from the residual cleaning composition. Blocking includes: ● Inhibition of pH-based interference from the cleaning composition (e.g. blocking pH changes that may be caused by residual cleaning compositions, which are typically basic); and/or ● Inhibition of chelation interference commonly elicited by the cleaning composition (e.g. blocking chelators from binding to metal species, such as iron); and/or ● Inhibition of surfactant interference from the cleaning composition (e.g. blocking surfactants from interfering with the colour changing agent, such as CAS). ● Inhibition of hypochlorite interference from the cleaning composition (e.g. blocking reactive hypochlorites from degrading the colour changing agent, such as CAS). ● Inhibition of acid interference (e.g. acetic acid interference) from the cleaning composition (e.g. blocking pH changes that may be caused by residual cleaning compositions, which are typically acidic). ● Inhibition of peroxide interference from the cleaning composition (e.g. blocking reactive peroxides from interfering with the colour change composition by modulating the pH of reactive species). ● Inhibition of peracetic acid interference from the cleaning composition (e.g. blocking reactive peracetic acid from interfering with the colour change composition by modulating the pH of reactive species). The buffer included in the blocking composition can be defined by a buffer capacity and working pH range. These ranges help to ensure that the blocking of undesired surface pH changes can be achieved, whilst also helping to ensure they are not too high to prevent colour change caused by drastic pH changes (e.g. an excessive level of bleach). The buffer capacity should preferably have a β of around -0.7 to 0.7 mol. The working pH should preferably be around pH 2.6 to pH 8, optionally pH 3 to pH 8, optionally pH 3.5 to 7, optionally around 5.6 to 7. Chelation inhibitors included in the buffers must have an affinity for relevant chelation molecules (e.g. EDTA or Citrate). This ensures that they bind to the relevant chelator to block the chelator from interacting with the colour change composition. The affinity can be measured with binding constants (log K _d ) and range from 4 - 42. Variation in K _d can be modulated via relative abundance. Surfactants included in the buffer must be present at such a level as to prevent cleaning chemicals (e.g. quaternary ammonium compounds) from causing an undesired colour change. This can be defined as preventing a specific number of molecules from causing a colour change that would indicate a ‘contaminated’ results and is measured in parts per million (ppm). The range that the surfactant may inhibit is 5 - 2000 ppm. Where the amount of a particular component in a composition or solution is expressed as a “wt%”, this is based on the total weight of composition or solution and may be: As used herein, when said blocking composition comprises a certain concentration of said one or more metals, this refers to the amount of free metallic species in the composition (e.g. dissociated metal species from a precursor salt). When multiple metals exist (e.g. the composition comprises both ZnCl ₂ and CaCl ₂ ) then the defined concentration refers to the sum of concentrations of these species. The term “binding affinity” is intended to refer to the strength of the binding interaction between a molecule to its ligand/binding partner. Binding affinities for various chemical species are typically found in textbooks, such as Microchimica Acta 1964, 52, 414–428. As used herein, the term “buffer capacity” is intended to refer to a quantitative measure of the amount a buffer can resist changes in pH. Capacities for various buffers can be measured in accordance with ISO 23497:2019 (https://www.iso.org/obp/ui#iso:std:iso:23497:ed-1:v1:en, accessible as of 24 January 2022). In certain aspects, present disclosures refer to different compositions, such as a “blocking composition”, a “colour changing composition”, a “detergent composition” or a “sprayable composition”. These different compositions can comprise similar components, such as surfactants. For the sake of clarity and to avoid confusion, a surfactant present in a blocking composition is referred to herein as a “blocking composition surfactant”, a surfactant present in a colour changing composition is referred to herein as a “colour changing composition surfactant”, etc. while a metal present in a blocking composition is referred to herein as a “blocking composition metal”. These are merely labels intended to aid understanding. DETAILED DESCRIPTION According to a first aspect, provided herein is a substrate (optionally a sponge) comprising a blocking composition, wherein said blocking composition comprises: one or more metals; one or more blocking composition surfactants; and one or more pH buffers; wherein 0.375 ml of said composition comprises a combined amount of said metal and said blocking composition surfactant that is sufficient to block: at least around 1.372 x 10 ^-8 moles of n-alkyl dimethyl benzyl ammonium chloride; and at least around 3.106 x 10 ^-8 moles of C ₉ -C ₁₁ alkyl alcohol ethoxylate. The blocking composition defined herein has been specifically formulated to block cleaning compositions which are typically found in a variety of settings (e.g. those found in food hygiene settings, medical settings, and the like). The amounts of metal, surfactant and other components mentioned below have been specifically chosen bearing these in mind. In the examples that follow, numerous such cleaning compositions are tested (e.g. the “D10” base formulation which commonly comprises a number of cleaning compositions). The substrate is useful in applications in which detection of microorganisms is desirable. The substrate can be wiped over an article (e.g. a surface) to block action of residual cleaning chemicals. Thereafter, the article/surface may be ready for sampling and detection of microorganisms by means of a colour change using a colour changing composition (such as a colour changing composition defined herein, e.g. one which operates via an interaction between the colour changing agent, metal and siderophores as released by microorganisms, such as bacteria). The blocking composition may comprise a combined amount of said metal and said blocking composition surfactant that is sufficient to block: at least around 2.854x10 ^-7 , optionally at least around 5.57x10 ^-7 , optionally at least around 8.287x10 ^-7 , optionally at least around 1.1x10 ^-6 , optionally around 1.3x10 ^-6 , such as around 1.372x10 ^-6 moles of n-alkyl dimethyl benzyl ammonium chloride; and at least around 6.46x10 ^-7 , optionally at least around 1.261x10 ^-6 , optionally at least around 1.876x10 ^-6 , optionally at least around 2.491x10 ^-6 , optionally around 3.1x10 ^-6 such as around 3.106x10 ^-6 moles of C ₉ -C ₁₁ alkyl alcohol ethoxylate. The blocking composition may comprise a combined amount of said metal and said blocking composition surfactant that is sufficient to block: at most around 1.372 x 10 ^-4 , optionally at most around 1.1x10 ^-4 , optionally at most around 8.287x10 ^-5 , optionally at most around 5.57x10 ^-5 , optionally at most around 2.854x10 ^-5 , around 1.3x10 ^-6 , such as around 1.372x10 ^-6 moles of n-alkyl dimethyl benzyl ammonium chloride; and at most around 3.106 x 10 ^-4 , optionally at most around 2.491x10 ^-4 , optionally at most around 1.876x10 ^-4 , optionally at most around 1.261x10 ^-4 , optionally at most around 6.46x10 ^-5 , optionally around 3.1x10 ^-6 such as around 3.106x10 ^-6 moles of C ₉ -C ₁₁ alkyl alcohol ethoxylate. The blocking composition may comprise a combined amount of said metal and said blocking composition surfactant that is sufficient to block: around 1.372x10 ^-8 to 1.372x10 ^-4 , optionally around 2.854x10 ^-7 to 1.1x10 ^-4 , optionally around 5.57x10 ^-7 to 8.287x10 ^-5 , optionally around 8.287x10 ^-7 to 5.57x10 ^-5 , optionally around 1.1x10 ^-6 to 2.854x10 ^-5 moles of n-alkyl dimethyl benzyl ammonium chloride; and around 3.106x10 ^-8 to 3.106x10 ^-4 , optionally around 6.46x10 ^-7 to 2.491x10 ^-4 , optionally around 1.261x10 ^-6 to 1.876x10 ^-4 , optionally around 1.876x10 ^-6 to 1.261x10 ^-4 , optionally around 2.491x10 ^-6 to 6.46x10 ^-5 moles of C ₉ -C ₁₁ alkyl alcohol ethoxylate. The blocking composition may comprise a combined amount of said metal and said blocking composition surfactant that is sufficient to block said amount of n-alkyl dimethyl benzyl ammonium chloride and C ₉ -C ₁₁ alkyl alcohol ethoxylate, and at least: (a) around 1.937x 10 ^-8 moles of citrate, optionally at least around 4.029x10 ^-7 , optionally at least around 7.864x10 ^-7 , optionally at least around 1.17x10 ^-6 , optionally at least around 1.553x10 ^-6 , optionally around 1.9x10 ^-6 such as around 1.937x10 ^-6 ; and/or (b) around 2.959 x 10 ^-8 moles of ethylenediaminetetraacetic acid (EDTA), optionally at least around 6.155x10 ^-7 , optionally at least around 1.201x10 ^-6 , optionally at least around 1.787x10 ^-6 , optionally at least around 2.373x10 ^-6 , optionally around 3x10 ^-6 such as around 2.959x10 ^-6 . Citrate and EDTA are commonly-encountered chelators present in a variety of cleaning compositions and it is desirable, in some implementations, to provide a blocking composition which is able to cater for such chelators. The blocking composition may comprise a combined amount of said metal and said blocking composition surfactant that is sufficient to block said amount of n-alkyl dimethyl benzyl ammonium chloride and C ₉ -C ₁₁ alkyl alcohol ethoxylate, and at most: (a) around 1.937x10 ^-4 moles of citrate, optionally at most around 1.553x10 ^-4 , optionally at most around 1.17x10 ^-4 , optionally at most around 7.864x10 ^-5 , optionally at most around 4.029x10 ^-5 , optionally around 1.9x10 ^-6 such as around 1.937x10 ^-6 ; and/or (b) around 2.959x10 ^-4 moles of ethylenediaminetetraacetic acid (EDTA), optionally at most around 2.373x10 ^-4 , optionally at most around 1.787x10 ^-4 , optionally at most around 1.201x10 ^-4 , optionally at most around 6.155x10 ^-5 , optionally around 3x10 ^-6 such as around 2.959x10 ^-6 . The blocking composition may comprise a combined amount of said metal and said blocking composition surfactant that is sufficient to block said amount of n-alkyl dimethyl benzyl ammonium chloride and C ₉ -C ₁₁ alkyl alcohol ethoxylate, and: (a) around 1.937 x 10 ^-8 to 1.937 x 10 ^-4 , optionally around 4.029x10 ^-7 to 1.553x10 ^-4 , optionally around 7.864x10 ^-7 to 1.17x10 ^-4 , optionally around 1.17x10 ^-6 to 7.864x10 ^-5 , optionally around 1.553x10 ^-6 to 4.029x10 ^-5 moles of citrate; and/or (b) around 2.959 x 10 ^-8 to 2.959 x 10 ^-4 , optionally around 6.155x10 ^-7 to 2.373x10 ^-4 , optionally around 1.201x10 ^-6 to 1.787x10 ^-4 , optionally around 1.787x10 ^-6 to 1.201x10 ^-4 , optionally around 2.373x10 ^-6 to 6.155x10 ^-5 moles of ethylenediaminetetraacetic acid (EDTA). According to a second aspect, provided herein is a substrate (optionally a sponge) comprising a blocking composition (optionally around 0.15 ml thereof), wherein said blocking composition comprises: one or more metals; one or more blocking composition surfactants; and one or more pH buffers, optionally wherein: around 0.15 ml of the blocking composition comprises a combined amount of said metal and said blocking composition surfactant that is sufficient to block: (a) at least around 1.937 x 10 ^-8 moles of citrate, optionally at least around 4.029x10- ⁷ , optionally at least around 7.864x10 ^-7 , optionally at least around 1.17x10 ^-6 , optionally at least around 1.553x10 ^-6 , optionally around 1.9x10 ^-6 such as around 1.937x10 ^-6 ; and/or (b) at least around 2.959 x 10 ^-8 moles of ethylenediaminetetraacetic acid (EDTA), optionally at least around 6.155x10 ^-7 , optionally at least around 1.201x10 ^-6 , optionally at least around 1.787x10 ^-6 , optionally at least around 2.373x10 ^-6 , optionally around 3x10 ^-6 such as around 2.959x10 ^-6 ; and/or (c) at most around 1.937 x 10 ^-8 moles of citrate, optionally at most around 1.553x10- ⁴ , optionally at most around 1.17x10 ^-4 , optionally at most around 7.864x10 ^-5 , optionally at most around 4.029x10 ^-5 , optionally around 1.9x10 ^-6 such as around 1.937x10 ^-6 ; and/or (d) at most around 1.937 x 10 ^-4 moles of ethylenediaminetetraacetic acid (EDTA), optionally at most around 2.373x10 ^-4 , optionally at most around 1.787x10 ^-4 , optionally at most around 1.201x10 ^-4 , optionally at most around 6.155x10 ^-5 , optionally around 3x10 ^-6 such as around 2.959x10 ^-6 ; and/or (e) around 1.937 x 10 ^-8 to 1.937 x 10 ^-8 moles of citrate, optionally around 4.029x10 ^-7 to 1.553x10 ^-4 , optionally around 7.864x10 ^-7 to 1.17x10 ^-4 , optionally around 1.17x10 ^-6 to 7.864x10 ^-5 , optionally around 1.553x10 ^-6 to 4.029x10 ^-5 ; and/or (f) around 2.959 x 10 ^-8 to 1.937 x 10 ^-4 moles of ethylenediaminetetraacetic acid (EDTA), optionally around 6.155x10 ^-7 to 2.373x10 ^-4 , optionally around 1.201x10 ^-6 to 1.787x10 ^-4 , optionally around 1.787x10 ^-6 to 1.201x10 ^-4 , optionally around 2.373x10 ^-6 to 6.155x10 ^-5 . Features below relate to the first and/or second aspects. The one or more metals may comprise one or more metal salts selected from the group consisting of metal halides (optionally metal chlorides, metal fluorides, metal iodides and metal bromides), metal acetates, metal sulphates, metal carbonates, metal nitrates, metal phosphates, metal gluconates, metal oxides, metal hydroxides, metal citrates, metal lactates, metal glubionates, metal hydrates, metal peroxides, metal hypochlorides, metal dioxides and metal fumarates. The one or more metals may be selected from the group consisting of zinc, calcium, magnesium, copper, bismuth, indium, manganese, nickel, titanium, chromium, aluminum, lithium, sodium, potassium, beryllium, radium, scandium, yttrium, lanthanum, vanadium, iron, cobalt, iridium, hafnium, silver, thallium, palladium, cadmium, tin, gallium, cerium, praseodymium, neodymium, promethium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium, americium, curium, berkelium, californium, thorium, uranium and neptunium, optionally from the group consisting of magnesium, calcium, zinc, copper, bismuth and manganese, or metal ions thereof, optionally from the group consisting of magnesium, calcium and zinc, or metal ions thereof. The one or more metals may be selected from the group consisting of Zn ²⁺ , Ca ²⁺ , Mg ²⁺ , Cu ²⁺ , Cu ³⁺ , Zn ³⁺ , Bi ³⁺ , In ³⁺ , Mn ²⁺ , Mn ³⁺ , Ni ²⁺ , Ti ³⁺ , Cr ³⁺ , Al ³⁺ , Li ⁺ , Na ⁺ , K ⁺ , Be ²⁺ , Sr ²⁺ , Ba ²⁺ , Ra ²⁺ , Sc ³⁺ , Y ³⁺ , La ³⁺ , V ²⁺ , Cr ²⁺ , Fe ²⁺ , Co ²⁺ , V ³⁺ , Ir ⁴⁺ , Hf ⁴⁺ , VO ²⁺ , Ag ⁺ , Tl ⁺ , Pd ²⁺ , Cd ²⁺ , Sn ²⁺ , Ga ³⁺ , Tl ³⁺ , Ce ³⁺ , Pr ³⁺ , Nd ³⁺ , Pm ³⁺ , Sm ³⁺ , Eu ³⁺ , Gd ³⁺ , Tb ³⁺ , Dy ³⁺ , Ho ³⁺ , Er ³⁺ , Tm ³⁺ , Yb ³⁺ , Lu ³⁺ , Am ³⁺ , Cm ³⁺ , Bk ³⁺ , Cf ³⁺ , Th ⁴⁺ , U ⁴⁺ and Np ⁴⁺ (optionally Zn ²⁺ , Ca ²⁺ , Mg ²⁺ , Cu ²⁺ , Cu ³⁺ , Bi ³⁺ , In ³⁺ , Mn ²⁺ , Mn ³⁺ , Ni ²⁺ , Ti ³⁺ , Cr ³⁺ , Al ³⁺ , Li ⁺ , Na ⁺ , K ⁺ , Be ²⁺ , Sr ²⁺ , Ba ²⁺ , Ra ²⁺ , Sc ³⁺ , Y ³⁺ , La ³⁺ , V ²⁺ , Cr ²⁺ , Fe ²⁺ , Co ²⁺ , V ³⁺ , Ir ⁴⁺ , Hf ⁴⁺ , VO ²⁺ , Ag ⁺ , Tl ⁺ , Pd ²⁺ , Cd ²⁺ , Sn ²⁺ , Ga ³⁺ , Tl ³⁺ , Ce ³⁺ , Pr ³⁺ , Nd ³⁺ , Pm ³⁺ , Sm ³⁺ , Eu ³⁺ , Gd ³⁺ , Tb ³⁺ , Dy ³⁺ , Ho ³⁺ , Er ³⁺ , Tm ³⁺ , Yb ³⁺ , Lu ³⁺ , Am ³⁺ , Cm ³⁺ , Bk ³⁺ , Cf ³⁺ , Th ⁴⁺ , U ⁴⁺ and Np ⁴⁺ ), optionally from the group consisting of Zn ²⁺ , Ca ²⁺ , Mg ²⁺ , Cu ²⁺ , Cu ³⁺ , Zn ³⁺ , Bi ³⁺ , Mn ²⁺ and Mn ³⁺ (optionally Zn ²⁺ , Ca ²⁺ , Mg ²⁺ , Cu ²⁺ , Cu ³⁺ , Bi ³⁺ , Mn ²⁺ and Mn ³⁺ ), optionally from the group consisting of Zn ²⁺ , Ca ²⁺ and Mg ²⁺ . The one or more metals may comprise magnesium, optionally Mg ²⁺ . The one or more metals may comprise calcium and zinc (optionally Ca ²⁺ and Zn ²⁺ ). To reduce the interaction of chelators with iron and prevent the degradation of the colour changing complex, magnesium chloride was added to the blocking composition. Magnesium competes with iron in binding to the chelators and thus it reduces chelator-iron interaction. Although iron has a higher binding affinity to EDTA (log Kf = 25.1) or citrate, the binding to magnesium (EDTA binding: log Kf = 8.79) can be enhanced by adding an excess of magnesium ions to iron ions. An advantage of using magnesium chloride is that it has a low binding affinity towards the dye and siderophore molecules preventing a significant interference with the colour changing composition. The one or more metals may comprise zinc and/or calcium, optionally Zn ²⁺ and/or Ca ²⁺ , optionally ZnCl₂ and/or CaCl₂. Calcium chloride was also investigated to block chelator-iron interactions. Compared to magnesium ions, calcium ions have a higher binding affinity to EDTA (log K _f = 10.69). Zinc chloride showed a greater efficiency in blocking chelator-iron interactions as it has been published to have a higher binding affinity (EDTA binding: log K _f = 16.5). Copper chloride has a higher binding affinity to EDTA (log Kf = 18.8) and was tested as a chelator inhibitor. It has shown initial results in blocking chelator-iron interaction, but it also represented an issue in binding to the dye molecule and influencing the colour changing complex. Both bismuth (III) salts (EDTA binding: log Kf = 27.8) and manganese (III) salts (EDTA binding: log Kf = 25.3) were also investigated as potential chelator inhibitors due to their high binding affinities to EDTA. Said blocking composition may comprise around 0.0001 to 4 M of said one or more metals, optionally around .001 to 2 M; optionally 0.01 to 1 M, optionally around 0.05 to 0.25 M, optionally around 0.1 to 0.15 M. The one or more metals comprise ZnCl ₂ and CaCl ₂ . Optionally, said blocking composition comprises: around 0.00005 to 2 M of ZnCl ₂ , optionally around 0.001 to 1 M; optionally 0.005 to 0.05 M, optionally around 0.025 to 0.125 M, optionally around 0.05 to 0.1 M, optionally around 0.06 to 0.08 M; and around 0.00005 to 2 M of CaCl ₂ , optionally around 0.001 to 1 M; optionally 0.005 to 0.05 M, optionally around 0.025 to 0.125 M, optionally around 0.03 to 0.07 M, optionally around 0.04 to 0.06 M. The one or more metals may have a binding affinity, log K _f , for EDTA of at least around 4, optionally at least around 8, 10, 15, 20, 25, 30, 35, 40 or 45. Said metal may have a binding affinity, log Kf, for EDTA of at most around 30, optionally at most around 25, 20, 15, 10 or 8. Said metal may have a binding affinity, log K _f , for EDTA of around 4 to 45, optionally around 8 to 30, optionally around 10 to 30, optionally around 15 to 25, optionally around 20 to 25. The metal may have a binding affinity for Chromeazurol S of at most 15, optionally at most 13, optionally at most 10 (e.g. per Microchimica Acta 1964, 52, 414–428, log K by the method of Dey et al.). The one or more pH buffers may be selected from the group consisting of: glycine, acetate, citrate, phosphate, ethanesulfonic acid and bis-tris methane. The one or more pH buffers may be selected from the group consisting of: glycine-hydrochloric acid, sodium acetate (counter-substrate acetic acid), piperazine-N,N′-bis(2-ethanesulfonic acid), citrate, phosphate, phosphate-citrate, 2-(N-morpholino)ethanesulfonic acid, 3-(N- morpholino)propanesulfonic acid and bis-tris methane. The one or more pH buffers may provide a pH working range of about 2.6 to 7, optionally 3.5 – 5.8, optionally around 5.6 – 7. The one or more pH buffers may provide a buffer capacity (β, beta) of about -0.7 to 0.7 mol, optionally about -0.4 to 0.7 mol, optionally about -0.1 to 0.7 mol, optionally about 0.075 to 0.7 mol, optionally about 0.04 to 0.7 mol; and/or optionally about -0.7 to 0.4 mol, optionally about -0.7 to 0.1 mol, optionally about -0.7 to 0.04 mol, optionally about -0.7 to 0.01 mol; and/or optionally about -0.06 to 0.04, optionally about -0.05 to 0.02, optionally about -0.04 to 0.015 mol. pH was determined to influence the formation of distinct colour changing complexes and their activity. Glycine buffer was tested due to its ability to keep a strong acidic pH (3.5) preventing the rise of pH after the addition of the highly basic detergents. The red colour change was achieved by the release of the protonated dye molecule upon reaction. It was also observed that glycine might have had a catalysing effect on the more stable colour changing complexes. Although the low pH was beneficial in blocking chelator-iron interactions because of protonation, it simultaneously lowered the favoured siderophore-iron interactions. Acetate buffer was investigated as an alternative buffering system due to its greater buffering range of pH 3.6 - 5.8. The blocking composition may comprise around 10 mmol/L to 1 mol/L of said one or more pH buffers, optionally around 10 mmol/L to 750 mmol/L, optionally around 50 mmol/L to 500 mmol/L, optionally around 100 mmol/L to 300 mmol/L, optionally around 100 to 200 mmol/L, optionally around 130 to 170 mmol/L. The one or more pH buffers may comprise acetate, such as sodium acetate. The blocking composition surfactant may comprise: a polysorbate (optionally a polysorbate 20, polysorbate 40, polysorbate 60, polysorbate 80 or optionally a Tween ^TM ) optionally at a level of around 1 mM to 200 mM; an aliphatic phenol ethoxylate (such as Triton X-100 ^TM ; optionally wherein the aliphatic group is an alkyl group; optionally wherein the aliphatic phenol ethoxylate is octyl phenol ethoxylate) optionally at a level of around 0.1 mM to 200 mM; a cyclodextrin (optionally β-cyclodextrin or γ-cyclodextrin); an aliphatic sulfate (optionally wherein the aliphatic group is an alkyl group, optionally wherein the aliphatic group has a straight chain length of 8 to 16 carbon atoms; optionally wherein the aliphatic sulfate is sodium dodecyl sulfate) optionally at a level of around 0.005 mM to 200 mM; a lecithin optionally at a level of around 1 mM to 250 mM; or a pyruvate (optionally an alkyl pyruvate, sodium pyruvate or pyruvic acid, optionally wherein the alkyl pyruvate is methyl pyruvate) optionally at a level of around 1 mM to 10000 mM; a branched-chain or single-chain aliphatic carbonate (optionally wherein the aliphatic group is an alkyl group, optionally wherein the aliphatic group has a chain length of 8 to 22 carbon atoms, optionally 8 to 18 carbon atoms); a branched-chain or single-chain aliphatic benzene sulphonate (optionally wherein the aliphatic group is an alkyl group, optionally wherein the aliphatic group has a chain length of 8 to 16 carbon atoms, optionally 10 to 14 carbon atoms); a branched-chain or single-chain polyetheramine; or a cocamide diethanolamine (DEA) (optionally comprising a carbon chain having a chain length of 8 to 18 carbon atoms, optionally 8 to 12 carbon atoms). Polysorbate (e.g. polysorbate-80) may be helpful to block action of QATs, which are present in a variety of cleaning compositions. Its large structural composition can incorporate the QAT molecule and prevent it from interacting with the colour changing complex. Inclusion of polysorbates (e.g. polysorbate-80) may be helpful to reduce friction of absorbent materials (e.g. sponges/swabs/tips) with target surfaces. Polysorbate surfactants are not believed to interact with the colour changing agent (such as the chrome azurol family, e.g. CAS or CAB). An additional advantage of including surfactant-like molecules in the blocking composition is the minimisation of surface tension between the analysed surface and substrate (e.g. the swab/a cotton tip thereof). Similarly, cyclodextrin substrates were tested due to their ability to incorporate QAT molecules and thus to inhibit their interaction with the colour changing system. Triton was included in the blocking composition due to its reported property to lyse bacterial membrane. It was hypothesised that the triton molecules would lyse bacteria and release internal siderophores leading to an increase in siderophore sample size and thus enhancing the sensitivity of the colour changing system. It was also observed that triton molecules influenced colour. SDS is known in the literature to have a higher efficiency in lysing cellular membranes. Due to its structural differences to the glycol-based polysorbate and triton substrates, SDS was studied to also have a higher stability in acidic media. In contrast to the previously investigated substrates, SDS has a negatively charged head allowing it to interact with the positively charged surfactant molecules of certain dye complexes (e.g. CAS) and thus catalysing the desired reaction, resulting in increased overall sensitivity . Lecithin was found to be extremely effective in limiting QAT interaction. Pyruvate substrates were used to deactivate the active component of the bleach cleaners. Pyruvate chemicals react with peroxides and hypochlorites leading to decarboxylation and thus their decomposition. The blocking composition surfactant may comprise an aliphatic sulfate (optionally wherein the aliphatic group is an alkyl group, optionally wherein the aliphatic group has a chain length of 8 to 16 carbon atoms (optionally straight chain); optionally wherein the aliphatic sulfate is sodium dodecyl sulfate). The blocking composition surfactant (selected from the options above, optionally sodium dodecyl sulfate) may be present from an amount of about 1 x 10 ^-5 mol/L to about 3.5 x 10 ^-3 mol/L, optionally about 1 x 10 ^-4 mol/L to about 3.5 x 10 ^-3 mol/L, optionally about 2.059x10 ^-4 to 2.926x10 ^-3 , optionally about 3.119x10 ^-4 to 2.352x10 ^-3 , optionally about 4.178x10 ^-4 to 1.778x10 ^-3 , optionally about 5.238x10 ^-4 to 1.204x10 ^-3 . The blocking composition may further comprise 5-sulfosalicyclic acid. As discussed above, SDS was partially used as a catalyst to accelerate the desired colour change of the colour changing system because of its ability to interact with the surfactant component of the complex. An alternative way of catalysing the reaction was found to be the addition of 5-sulfosalicyclic acid. 5-sulfosalicyclic acid may be present from an amount of about 5x10 ^-3 mol / L, optionally 1 x10 ^-6 mol / L to 2.5x10 ^-3 mol / L, optionally, 1x10 ^-4 mol / L to 1x10 ^-3 mol / L. The blocking composition may comprise: ● around 12.5-400 mM acetate buffer at a pH of around 5.6 – 5.8 ● around 0.05-3.5 mM SDS ● around 10-200 mM CaCl ₂ ● around 15-250 mM ZnCl ₂ Around 0.05 ml to 0.5 ml of said blocking composition (e.g. around 0.1 ml to 0.4 ml, optionally around 0.125 ml to 0.3 ml) of such a composition may be present on in the substrate (e.g. on a swab tip/sponge). The substrate carrier may be an absorbent material, such as a sponge or wipe. The substrate may be able to absorb at least about 5 times its weight (based on the total weight of the substrate) in liquid, such as at least about 7 its weight, such as at least about 10 times its weight, such as at least about 15 times its weight in liquid, such at least about 20 times its weight in liquid. The substrate may be able to absorb between about 4-40 times its weight (based on the total weight of the substrate) in liquid, such as between about 4-30 times its weight, such as between about 4-20 times its weight in liquid. The substrate may be a non-woven material, such as a fibrous material (optionally paper or a fabric). The substrate carrier may comprise cellulose (e.g. derived from cotton), polyester, lignin, protein, acrylic, nylon, aramid, polyurethane, alginate and/or mixtures thereof (such as lignocellulosic fibres). The substrate carrier may comprise fibres of cellulose, polyester, lignin and/or mixtures thereof (such as lignocellulosic fibres), optionally wherein the substrate carrier comprises cellulose. The substrate carrier may comprise pulp, wool, silk, jute, linen, ramie, sisal, bagasse, banana fibres, hemp, flax, camel hair, kenaf and/or mixtures thereof. The substrate may be a wipe, such as a surface wipe or a personal (e.g. baby) wipe; a paper towel; or a tissue (such as a toilet or facial tissue). The substrate may be a surface wipe. According to a third aspect, provided herein is a composition comprising the blocking composition as defined in the first or second aspect. In other words, the third aspect is not limited to the presence of a substrate. The composition may comprise: around 0.001 to 3 mol / L of one or more metals selected from the group consisting of: Zn ²⁺ , optionally wherein said one or more metals are selected from the group consisting of: Zn ²⁺ , Ca ²⁺ , Mg ²⁺ , Cu ²⁺ , Cu ³⁺ , Bi ³⁺ , Mn ²⁺ , Mn ³⁺ , Cr ²⁺ , Cr ³⁺ , Li ⁺ , K ⁺ , Na ⁺ , Be ²⁺ and Fe ²⁺ ; optionally wherein said one or more metals are selected from the group consisting of: Zn ²⁺ , Ca ²⁺ , Mg ²⁺ , Fe ²⁺ , Cr ³⁺ , Mn ²⁺ and Bi ³⁺ . The one or more metals may be present as salts selected from the group consisting of: metal halides, metal acetates, metal carbonates and metal sulfates. The composition may comprise around 10 to 750 mmol / L of buffer selected from the group consisting of: acetate, glycine, ethanesulfonic acid, bis-tris methane and phosphate. The composition may comprise: (a) around 0.01 to 10 mmol / L of an aliphatic sulfate; or (b) around 1 to 100 mmol / L of an aliphatic phenol ethoxylate; or (c) around 10 to 200 mmol / L of a polysorbate. The composition may comprise around 0.001 to 2 mol / L of the one or more metals, optionally around 0.001 to 1.5 mol / L, optionally around 0.001 to 1 mol / L. The composition may comprise: around 0.001 to 3 mol / L of one or more metals are selected from the group consisting of: Zn ²⁺ , Ca ²⁺ , Mg ²⁺ , Fe ²⁺ , Cr ³⁺ , Mn ²⁺ and Bi ³⁺ ; around 10 to 750 mmol / L buffer (optionally according to claim 25, optionally acetate buffer); around 0.01 to 10 mmol / L surfactant (optionally an aliphatic sulfate, optionally sodium dodecyl sulfate). The buffer may be selected from the group consisting of: acetate, glycine, ethanesulfonic acid, bis-tris methane and phosphate; optionally wherein the buffer is acetate. The surfactant may be an aliphatic sulfate (optionally sodium dodecyl sulfate). The one or more metals may be Zn ²⁺ and Ca ²⁺ . The composition may comprise: around 0.001 to 1.5 mol / L of Zn ²⁺ ; around 0.001 to 1.5 mol / L of Ca ²⁺ . The composition may comprise: around 10 to 750 mmol / L of acetate buffer. The composition may comprise: around 0.01 to 10 mmol / L of an aliphatic sulfate. The composition may comprise: around 0.001 to 1 mol / L of Zn ²⁺ ; around 0.001 to 1 mol / L of Ca ²⁺ ; around 12.5 to 400 mmol / L of acetate buffer; around 0.05 to 3.5 mmol / L sodium dodecyl sulfate. The composition may comprise: around 15 to 250 mmol / L of Zn ²⁺ ; around 10 to 200 mmol / L of Ca ²⁺ ; around 12.5 to 400 mmol / L of acetate buffer; around 0.05 to 3.5 mmol / L sodium dodecyl sulfate. The one or more metals may comprise CaCl ₂ and ZnCl ₂ ; and the blocking composition may comprise a sodium dodecyl sulfate blocking composition surfactant. The composition may comprise: around 0.001 to 1.5 mol / L of ZnCl ₂ ; around 0.001 to 1.5 mol / L of CaCl ₂ ; around 10 to 750 mmol / L of acetate buffer; around 0.01 to 10 mmol / L sodium dodecyl sulfate. The composition may comprise: around 0.001 to 1 mol / L of ZnCl ₂ ; around 0.001 to 1 mol / L of CaCl ₂ . The composition may comprise around 12.5 to 400 mmol / L of acetate buffer. The composition may comprise around 0.05 to 3.5 mmol / L sodium dodecyl sulfate. The composition may comprise: around 12.5 to 400 mmol / L of acetate buffer; and around 0.05 to 3.5 mmol / L sodium dodecyl sulfate. The composition may comprise: around 0.001 to 1 mol / L of ZnCl ₂ ; around 0.001 to 1 mol / L of CaCl ₂ ; around 12.5 to 400 mmol / L of acetate buffer; around 0.05 to 3.5 mmol / L sodium dodecyl sulfate. The blocking composition may comprise: ● around 15 mmol/L to 250 mmol/L of ZnCl ₂ ; ● around 10 mmol/L to 200 mmol/L of CaCl ₂ ; ● around 12.5 mmol/L to 400 mmol/L acetate buffer; ● around 0.05-3.5 mM SDS. According to a fourth aspect, provided herein is a kit comprising: the substrate according to the first or second aspect and/or the composition according to the third aspect; and (a) a colour changing composition configured to detect the presence of microorganisms; and/or (b) a cleaning composition configured to reduce and/or eliminate microorganisms. The colour changing agent may be a chromeazurol (such as chromeazurol S or chromeazurol B) or a tannin. The colour changing agent may be chromeazurol S (CAS). Chromeazurol compounds form a blue colour upon binding with iron and are coloured orange in the absence of iron. It will be appreciated that chromeazurol compounds may exist in different isomeric forms and/or salt forms and/or de/protonated forms, particularly as a result of conjugation in the compounds. All such forms are envisaged herein. Tannins are a class of polyphenolic compounds, such as vescalagin, castalagin [(33beta)-isomer of vescalagin], penta-O-galloyl-beta-D-glucose. Said substrate may comprise around 0.05 ml to 0.75 ml of said blocking composition, optionally around 0.1 ml to 0.6 ml, optionally around 0.125 ml to 0.5 ml, optionally around 0.15 ml. Said colour changing composition may be present in an amount around 0.1 ml to 1 ml, optionally around 0.2 ml to 0.7 ml, optionally around 0.3 ml to 0.5 ml. Said colour changing composition may comprise: a colour changing composition metal, and a colour changing agent, wherein said colour changing composition metal is bindable to said colour changing agent to provide a change in colour on binding and/or release thereof. The kit may further comprise a colour changing composition surfactant, optionally wherein said colour changing composition metal and colour changing composition surfactant are present at a molar ratio of about 1:0.25 to 30, optionally about 1:0.5 to 7; optionally about 1:0.75 to 6; optionally about 1:0.75 to 5; optionally about 1:0.75 to 3; optionally about 1:1 to 3; optionally about 1:1 to 2.5. Said colour changing composition surfactant may be selected from the group consisting of: a polysorbate (optionally a polysorbate 80 or polysorbate 20, optionally a Tween ^TM ), an aliphatic phenol ethoxylate (such as Triton X-100; optionally wherein the aliphatic group is an alkyl group; optionally wherein the aliphatic phenol ethoxylate is octyl phenol ethoxylate), an aliphatic sulfobetaine (optionally wherein the aliphatic group is an alkyl group, optionally wherein the aliphatic group has a chain length of 8 to 16 carbon atoms (optionally straight chain); optionally wherein the aliphatic sulfobetaine is lauryl sulfobetaine), an aliphatic quaternary ammonium halide such as aliphatic trimethylammonium halide (optionally wherein the aliphatic group is an alkyl group, optionally wherein the aliphatic group has a chain length of 8 to 22 carbon atoms (optionally straight chain), optionally wherein the aliphatic trimethylammonium halide is myristyltrimethylammonium halide, trimethyloctadecylammonium halide, hexadecyl-trimethyl- ammonium bromide (HDTMA) or dodecyltrimethylammonium halide, optionally wherein the halide is a fluoride, bromide, or iodide), or combinations thereof, optionally wherein the aliphatic group has a chain length of 8 to 22 carbon atoms (optionally straight chain). Said colour changing composition metal and colour changing agent may be present at a molar ratio of about 1:0.25 to 25; optionally about 1:0.25 to 20, optionally 1:0.5 to 15; optionally about 1:1 to 13; optionally about 1:1.5 to 11.5; optionally about 1:1.5 to about 8; optionally about 1:1.5 to 5; optionally about 1:1.5 to 4; optionally about 1:1.5. Said colour changing composition metal may be iron; optionally iron (III); optionally FeCl ₃ , optionally hydrated FeCl ₃ (FeCl ₃ ⋅ ₆ H2O). Said colour changing agent may be chromeazurol (such as chromeazurol S or chromeazurol B) or a tannin, optionally wherein said colour changing agent is a chromeazurol, optionally chromeazurol S (CAS). The colour changing agent, such as CAS (e.g. CAS S) may be tetrabasic, having 4 ionizable hydrogen moieties. Ionization of one or more of said moieties may lead to a colour change. A chemical capable of achieving said ionization may therefore be detectable upon exposure to such a colour changing agent. The skilled person will understand the nature of chemicals for which the composition is suitable for detecting. The colour changing agent may provide a change in colour in the visible spectrum (e.g. about 390 nm to 700 nm). The colour changing composition may comprise: ● Fe : CAS : (C _10-16 )-trimethylammonium Bromide/Chloride ● Equivalent molar ratios around 1 : 1-3 : 0-4 (e.g.1-4) Around 0.1 ml to 1 ml (e.g. around 0.2 ml to 0.7 ml, optionally around 0.3 ml to 0.5 ml) of this solution may be used as the colour change liquid (e.g. in the swab reservoir). Said cleaning composition may comprise: an n-alkyl dialkyl triamine (e.g. wherein the n-alkyl is at least C ₁₀ , optionally C ₁₂ ; and/or optionally wherein the trialkyl is dipropylene, optionally wherein the n-alkyl dialkyl triamine is dodecyl dipropylene triamine), n-alkyl dimethyl ammonium halide (e.g. chloride), n-alkyl dialkyl aryl ammonium halide and aliphatic alcohol alkanoate, optionally wherein said n-alkyl dialkyl aryl ammonium halide is n-alkyl dimethyl benzyl ammonium halide, optionally n-alkyl dimethyl benzyl ammonium chloride, optionally wherein the n-alkyl is at least C ₈ (e.g. C ₈ - C ₁₀ ), optionally C ₁₂ -C ₁₆ n- alkyl dimethyl benzyl ammonium chloride; and/or wherein said aliphatic alcohol alkanoate is an alkyl alcohol alkanoate, optionally wherein the aliphatic group has a chain length of 8 to 18 carbon atoms (optionally straight chain), optionally wherein the aliphatic group has a chain length of 9 to 11 carbon atoms (optionally straight chain). Aliphatic alcohol alkanoate may be an alkyl alcohol ethoxylate, optionally C ₈ -C ₁₈ alkyl alcohol ethoxylate, optionally C ₉ -C ₁₁ alkyl alcohol ethoxylate. Said cleaning composition may comprise n-alkyl dialkyl aryl ammonium halide present from about 0.5 wt% to 15 wt%; and aliphatic alcohol alkanoate present from about 0.5 wt% to 15 wt%. Said cleaning composition may further compromise sodium hypochlorite present from about 1.8 wt% to 7 wt%, optionally 2.1 wt% to 6.5 wt%, optionally 2.4 wt% to 6 wt%, optionally 2.7 wt% to 5.5 wt%, optionally about 3 wt% to 5 wt%; and hydrogen peroxide present from about 10 wt% to 30 wt%; and peracetic acid present from about 10 wt% to 30 wt%; and acetic acid present from about 10 wt% to 30 wt%. Said cleaning composition may comprise: n-alkyl dialkyl aryl ammonium halide present from about 0.6 wt% to 14 wt%, optionally 0.7 wt% to 13 wt%, optionally 0.8 wt% to 12 wt%, optionally 0.9 wt% to 11 wt%, optionally about 1 wt% to 10 wt%; and aliphatic alcohol alkanoate present from about 0.6 wt% to 14 wt%, optionally 0.7 wt% to 13 wt%, optionally 0.8 wt% to 12 wt%, optionally 0.9 wt% to 11 wt%, optionally about 1 wt% to 10 wt%. Said cleaning composition may further comprise citrate present from about 0.5 wt% to 10 wt%; optionally about 0.6 wt% to 9 wt%, optionally 0.7 wt% to 8 wt%, optionally 0.8 wt% to 7 wt%, optionally 0.9 wt% to 6 wt%, optionally about 1 wt% to 5 wt%. Said cleaning composition may further comprise EDTA present from about 0.5 wt% to 30 wt%; optionally about 0.6 wt% to 25 wt%, optionally 0.7 wt% to 20 wt%, optionally 0.8 wt% to 15 wt%, optionally 0.9 wt% to 12.5 wt%, optionally, about 1 wt% to 10 wt%. Said cleaning composition may further comprise: sodium hypochlorite present from about 1.8 wt% to 7 wt%, optionally 2.1 wt% to 6.5 wt%, optionally 2.4 wt% to 6 wt%, optionally 2.7 wt% to 5.5 wt%, optionally about 3 wt% to 5 wt%; and hydrogen peroxide present from about 6 wt% to 42 wt%, optionally 7 wt% to 39 wt%, optionally 8 wt% to 36 wt%, optionally 9 wt% to 33 wt%, optionally, about 10 wt% to 30 wt%; and peracetic acid present from about 6 wt% to 42 wt%, optionally 7 wt% to 39 wt%, optionally 8 wt% to 36 wt%, optionally 9 wt% to 33 wt%, optionally about 10 wt% to 30 wt%; and acetic acid present from about 6 wt% to 42 wt%, optionally 7 wt% to 39 wt%, optionally 8 wt% to 36 wt%, optionally 9 wt% to 33 wt%, optionally about 10 wt% to 30 wt%. Said colour changing composition may comprise: a colour changing composition metal, wherein said colour changing composition is iron; a colour changing agent, wherein said colour changing agent is chromeazurol S; and optionally a colour changing composition surfactant, wherein said colour changing composition surfactant is dodecyltrimethylammonium bromide or dodecyltrimethylammonium chloride; wherein said iron is bindable to chromeazurol S to provide a change in colour on binding and/or release thereof, optionally wherein said iron and said aliphatic quaternary ammonium halide are present at a molar ratio of 1:0.25 - 1:5; and wherein said iron and said chromeazurol S are present at a molar ratio of 1:0.5 - 1:5. Said cleaning composition may comprise: n-alkyl dialkyl aryl ammonium halide, wherein said n-alkyl dialkyl aryl ammonium halide is n-alkyl dimethyl benzyl ammonium chloride present at an amount from about 1 wt% to 10 wt%; and aliphatic alcohol alkanoate, wherein said aliphatic alcohol alkanoate is C ₉ -C ₁₁ alkyl alcohol ethoxylate present at an amount from about 1 wt% to 10 wt%, optionally wherein said cleaning composition further comprises citrate, optionally present at an amount from about 1 wt% to 5 wt% and/or EDTA, optionally present at an amount from about 1 wt% to 10 wt%, optionally wherein said cleaning composition further compromises sodium hypochlorite, optionally present at an amount from about 3 wt% to 5 wt%; and/or hydrogen peroxide, optionally present at an amount from about 10 wt% to 30 wt%; and/or peracetic acid present at an amount from about 10 wt% to 30 wt%; and/or acetic acid present at an amount from about 10 wt% to 30 wt%. According to a fifth aspect, provided herein is a swab comprising a rod having a substrate according to the first or second aspect at an end thereof (e.g. at a tip thereof, optionally wherein the substrate comprises 0.15 ml of said composition), optionally further comprising a reservoir of a colour changing composition (e.g. at an opposite end to the tip end). The reservoir may be rupturable to release colour changing composition (e.g. to enable it to interact with the swab substrate/tip). The swab may be provided with a collecting vial, to facilitate mixture of the colour changing composition with the blocking composition on/around the substrate. According to a sixth aspect, provided herein is a use of a substrate according to the first or second aspect, the composition according to third aspect or a swab according to the fifth aspect to block a cleaning composition. According to a seventh aspect, provided herein is a method comprising contacting a surface, or a sample therefrom, that has been pre-treated with a cleaning composition with the composition of the third aspect. Also provided herein is a method comprising contacting a surface that has been pre-treated with a cleaning composition with a substrate according to the first or second aspect or a swab as defined in the fifth aspect, to collect a sample therefrom. The method may further comprise contacting said surface, or sample therefrom (e.g. collected by a substrate according to the first or second aspect or a swab as defined in the fifth aspect) with a colour changing composition as defined in the fourth aspect. It will be appreciated that the first, second, third, fourth, fifth, sixth and seventh aspects are interrelated. Thus, features described above in relation to one or more of the first, second, third, fourth, fifth, sixth and seventh aspects apply mutatis mutandis to the subject matter of any one or more of the other aspects as will be readily understood by a person of skill in the art. BRIEF DESCRIPTION OF FIGURES Figs.1 to 10: absorption spectroscopy traces. Figs.11 to 24: photographs of vials of compositions. Fig.25(A&B): absorption spectroscopy traces. Fig.26: pH vs absorbance measurements. EXAMPLES General In the experiments below, stock solutions of the colour changing complex (hereafter “stock colour changing complex solutions”) were prepared, including the following: ● FreshCheck Complexes: o Complexes are given in the ratio of Iron : dye : surfactant. The 3 letters preceding the ratios (e.g. FCH) indicate the metal ion : dye : surfactant/aggregation inhibitor. A key to different letters is here: ▪ Fe = F ▪ C = CAS ▪ H = Hexadecyl-TMA ▪ D = Dodecyl-TMA ▪ P = Dodecyl pyridinium ammonium chloride o Example 1: FCH 1 : 2 : 0 - 4 o Example 2: FCH 1 : 2 - 3 : 0 - 4 o Example 3: FCH 1 : 2 - 3 : 0 - 4 o Example 4: FCH 1 : 2 : 0 - 4 o Example 5: ▪ FCH 1 : 1 - 3 : 0 - 4 ▪ FCD 1 : 1 - 3 : 0 - 4 ▪ FCP 1 : 1 - 3 : 0 – 4 o Example 16: FCH/D 1 : 2 - 3 : 0 - 4 ● Commercial Suppliers: o D10 - Diversey – commercially available from Viking Direct; with the following composition: o Bioklenz (or “BioK”) – commercially available from Christeyns; with the following composition: o Hypochlorite (bleach) provided by Milton; with the following composition A composition representative of commonly used bleach (hereafter “bleach”) was prepared, including the following: ● Dilution to standard (i.e. sprayable) stock was around 11mL in 600mL A siderophore (deferoxamine)-based composition (hereafter “Dfx”) was prepared: ● Deferoxamine (DFX) was sourced from Merck (Sigma Aldrich). DFX was dissolved in MilliQ water at a stock concentration of 0.4994mM. This was diluted to 0.04994mM - 0.004994mM for studies. mM = mmol / L. Introduction To investigate the effects of cleaning chemicals on colour changing compositions, an analogue colour changing complex (based on iron-CAS-S colour changing dye as discussed above) was mixed with a range of chemicals (D10, BioK and bleach) typically found in commonly-available cleaning compositions, as well as siderophore-based composition Dfx. Light absorption spectroscopy measurements were conducted, and the results are shown in Fig.1. In the absence of cleaning chemicals, the colour changing complex adopts two distinct curves depending on the presence or absence of siderophores. In Fig.1, the solid curve (peak ~576 nm) depicts a metal-bound colour changing complex (absence of siderophores) and the dash-dot-dot curve (peak ~495 nm) depicts the protonated colour changing complex (presence of siderophores), wherein metal has been released from the colour changing complex through chelation by the siderophores. A colour change shift can therefore clearly be seen, depending on the presence or absence of siderophores, which in turn indicates the presence or absence of microbes. Interaction with common cleaning chemicals interferes with this process, leading to several new peaks/curves in the light spectrum and consequent contamination of the colour-change shift as can be seen in Fig.1. This was generally caused by (a) the release of the metal from the colour changing dye (small dashes curve, peak ~430 nm, indicating interaction with trialkylamine (TEA) cleaning chemical, in BioKlenz; and long dashes curve, peak ~427 nm, indicating interaction with bleach) or (b) the formation of a colour changing detergent complex (dotted line, peak ~690 nm) indicating interaction with quaternary ammonium compound, QAT, benzalkonium chloride in D10). To mitigate these interference effects, the cleaning chemicals were blocked (or in other words, the colour changing complexes were stabilised) using a series of agents as discussed below. Example 1 – glycine buffer blocking of bleach Glycine buffer at pH 3.0 – 4.0 (150 μl, 50 - 150 mM), bleach (100 μl), BioKlenz (100 μl) and D10 (100 μl) were added to the stock colour changing complex solution (400 μl). As shown in Fig.2, the buffer blocked bleach, as shown with the long dashes curve which no longer appears with a peak in the ~427 nm region and instead appears approximately aligned with solid metal-bound dye complex ~576 nm). Without wishing to be bound by theory, it is understood that blocking here occurred by inhibiting the increase of pH after the addition of bleach. However, blocking of the trialkylamine cleaning chemical (TEAs from BioKlenz) was not achieved – the release of free dye can still be seen in Fig.2 (short dashes curve, peak ~430 nm still present). Addition of glycine buffer also appeared not to substantially affect formation of the dye-detergent complex (dots curve, peak ~690 nm still present) caused by QAT benzalkonium chloride (from D10). Example 2(A) – metal blocking of TEAs As mentioned above, addition of TEAs to the stock colour changing complex solution led to chelation of the metal and release of free colour changing dye (see also solid curve with peak ~430 nm, Fig.3A). A range of metal salts was incorporated in an effort to bind to TEAs and mitigate interaction with the stock colour changing complex solution. As seen in Fig.3A, the addition of magnesium chloride to a mixture of the stock colour changing complex solution 400 μl and 100 μl BioKlenz (i.e. in the presence of TEAs) resulted in the stabilisation of the dye complex (low levels 0.006 to 0.008 mol/L of MgCl ₂ , dashes; high levels 0.014 – 0.018 mol/L of MgCl ₂ , dots; both with peaks ~595 to 650 nm)/blocking of TEAs. Example 2(B) – metal blocking of TEAs (combination effect with glycine buffer) A combination of glycine buffer solution 150 μl (containing 50 - 150 mM glycine and 0.006 – 0.008 mol/L magnesium chloride) was added to the stock colour changing complex solution (400 μl) and tested against the common cleaning chemicals (bleach 100 μl, BioK 100 μl and D10100 μl; see Fig.3B). The undesired release of free dye as result of TEA interaction was blocked (see short dashes curve, which no longer appears with a peak in the ~430 nm region). However, the QAT and TEA components of the common cleaning chemicals still undesirably interact with the dye complex, despite the combination of glycine buffer and magnesium chloride (both eliciting peaks~656 nm), e.g. forming a dye-QAT complex. Example 3 – polysorbate blocking of QATs Polysorbate-80 (0.02 – 0.05 mol/L) was incorporated in an effort to bind to QATs and mitigate interaction with the dye complex. Fig.4 shows the decay of the dye-QAT complex as the result of the interaction of Polysorbate-80 (long dash, with peak spread between ~490 and 690 nm). Example 4 – 5-sulfosaliyclic acid effect on colour changing speed To decrease the reaction time of the dye–siderophore interaction and hence induce a quicker colour change, 5-sulfosalicyclic acid was investigated as a potential catalyst. Without wishing to be bound by theory, it is believed that 5-sulfosalicyclic acid can enhance the reactivity of metal- dye (e.g. CAS)-surfactant complexes by competing with the colour changing dye and coordinating to metal (e.g. iron). It will be appreciated that a balance is needed, between inducing a quicker colour change on the one hand and degrading the complex on the other. Fig.5 shows the interaction of 5-sulfosalicyclic acid with the dye complex in the stock colour changing complex solution (400 μl). An increase in 5-sulfosalicyclic acid amount led to a decrease in the absorption peak (low level: 8 - 11E-05 mol/L, medium level: 0.0002 – 0.0008 mol/L, high level: 0.0008 – 0.0015 mol/L). Low levels of 5-sulfosalicyclic acid show no change to the peak, but medium to high levels show degradation of the peak. This suggests a limit for 5-sulfosalicyclic acid based on the degradation of the formulation. Example 5(A) – pH and acetate buffer effect on colour change In Example 1, glycine was used as a buffering agent, enabling the pH of the stock colour changing complex solution to be kept at low acidic pH (~3.5). The low pH enabled a number of factors: (i) pH blocking of basic detergents found in common cleaning compositions, (ii) regulation of the binding affinity (K _d ) of chelators to metal/iron in the presence of the dye; and (iii) influencing the colour of bound and unbound dye. It was determined that the low pH of the solution simultaneously lowered the binding affinity of siderophore molecules to metal/iron by protonating siderophore binding sites. Due to its pH range of 3.6 – 5.6, acetate buffer was next investigated as a potential buffer to stabilise the pH of the stock colour changing complex solution after the addition of a detergent. Fig.6 breaks down the interaction of 400 μl of the stock colour changing complex solution with a range of common cleaning chemicals (upper graph is with 100 μl D10; middle is with 100 μl BioK,; and lower is with 100 μl bleach-based cleaning formulation) in both the absence (milli-Q purified water, MQ, dashed curve) and the presence (solid curve) of an acetate buffer (150 μl, 100-200 mM at pH 5.4-5.8). As can be seen, in all instances the acetate buffer stabilises the colour changing complex against the tested common cleaning chemicals (i.e. blocks these) and prevents a following colour change to bright green or yellow. For each graph, photographs can be seen in the top left to depict vial colouring (vial colouring: upper graph: upper teal, lower blue; middle graph: upper yellow, lower green; lower graph: upper yellow, lower teal). Fig.7 depicts the results of experiments conducted to demonstrate the effect of acetate buffer (150 μl, 100 - 200 mM) on 400 μl of the specific colour changing composition described in the general exemplary section above having a molar ratio Fe : CAS : HDTMA of 1:2-3:2- (based on 4.99 * 10^-5 mol/L Fe in 400 μl) and the results are shown in Fig.7. As with Example 5(A) above, it can be seen that the presence of acetate buffer stabilises the colour changing complex against the tested cleaning chemicals/blocks those cleaning chemicals. Example 5(B) – pH, acetate buffer, polysorbate and magnesium chloride effect on colour change Further experiments tested the effect of the acetate buffer (150 μl, 25 - 125 mM) in combination with 400 μl of the specific colour changing composition described in the general exemplary section above, having a molar ratio Fe : CAS : HDTMA of 1:2-3:2-4 (based on 0.0499 mol/L Fe in 400 μl), polysorbate 80 (5-10 molar equivalents to Fe) and magnesium chloride (1-5 molar equivalents to EDTA). These were tested against common cleaning chemicals (bleach 100 μl, BioK 100 μl and D10100 μl) and the results are shown in Fig.8. As can be seen, the combination of all three components elicited particularly stable colour changing compositions, against all tested common cleaning chemicals. In other words, these were blocked. Example 6 – pH and glycine buffer concentration effect on colour change Comparisons of different glycine buffer concentrations (150 μl, 25-75 mM and 75-125 mM) and their blocking and catalysis effects can be seen in Figs.9 and 10. Excessive levels of glycine cause undesired degradation of the colour changing composition, providing an upper limit of sensitivity. However, if the concentration of buffer is too low, the buffering capacity may be too low to block cleaning chemicals as discussed above. Example 7 – pH effect on colour change The stability of the dye complex can be influenced by the pH of the buffer and thus its interaction with common cleaning chemicals can vary in the presence of the blocking composition. For this example, the following solution was used: *described in the general exemplary section above, here having a molar ratio Fe : CAS : dodecyl pyridinium bromide of 1:2 - 3:1 - 3 (based on 2.50 * 10^-5 mol/L Fe in 200 μl). A series of vials was prepared with the solution above, with D10 or Dfx at various dilution levels as indicated in the tables below. In the tables below, the layout of cells (upper left to lower right) matches the layout of vials in Figs.11(A) and (B). Fig.11(A) – vial layout: In general terms, the D101x vial had the deepest blue colour, extending progressively towards a green/teal colour at 0.1x with a notable change towards green/teal at 0.5x and below. Vials 0.9x to 0x remained approximately consistent with a green/teal colour similar to that of 0.1x. For Dfx, the 1x vial had a yellow colour, transitioning through green between 0.6 to 0.4x and on to teal at 0.1x. Fig.11(B) – vial layout: In general terms, the D101x vial had a blue-purple colour, extending progressively towards a blue colour at 0.5x, transitioning towards purple again at 0.4x with a notable change towards red at 0.3x and below. For Dfx, the 1x vial had an orange colour, transitioning progressively towards purple at 0.1x. As seen from the right side of Fig.11(B), a vial of D10 at 1x had a blue colour and a vial of Dfx at 1x had a yellow colour. As seen in Figs.11(A) and (B), the blocking effect of the buffer towards an intermediate amount of a QAT-based cleaners diminished by lowering the pH of the Acetate buffer from pH 5.6 (A) to 3.3 (B). Example 8 – metal ion effect on colour change Further analysis of the cleaner interactions with FCD revealed that by lowering the cleaning chemical concentration, the blocking composition was not as efficient in inhibiting chelator interaction as initially identified, resulting in a colour change from blue to green. Hence, alternative metal ions in higher concentrations were tested against the individual chelators such as EDTA (Fig.12) and citrate (Fig.13). For this example, the following solution composition was used: *described in the general exemplary section above, here having a molar ratio Fe : CAS : dodecyltrimethyl ammonium chloride of 1:2-3:1-3 (based on 2.50 * 10^-5mol/L Fe in 200 μl). A series of vials was prepared with the composition above, with EDTA or citric acid at various dilution levels as indicated in the tables below. In the tables below, the layout of cells (upper left to lower right) matches the layout of vials in Figs.12 and 13. Fig.12 – vial layout: Vials labelled with bold underlined text above had a blue colour (i.e. were not substantially affected by EDTA presence) while the remaining vials were green. Generally speaking, the ZnCl ₂ vials had the best performance with CaCl ₂ next, followed by MgCl ₂ . Deeper blues were generally encountered with lower equivalent levels of EDTA. By changing the metal ion from magnesium to calcium or zinc, the efficiency of chelator blocking was increased maintaining the blue colour changing composition colour at higher EDTA concentrations. Zinc chloride was the preferred chelator inhibitor as it blocked EDTA (1x to 0.001x) and was the most successful in blocking citrate. The middle row shows that CaCl ₂ can block EDTA up to around 0.5x (which is representative of around 0.5x a standard level of cleaning chemicals typically found in real-world applications). The bottom row shows that ZnCl ₂ can block EDTA (except when EDTA is encountered in very high concentrations). Fig.13 – vial layout: Vials labelled with bold underlined text above had a blue colour (i.e. were not substantially affected by citric acid presence/citric acid was blocked) while the remaining vials were green. The top row shows that MgCl ₂ is able to block/stabilise the colour change complex up to around 0.01x dilution. The middle row shows that CaCl ₂ can block citric acid to approximately the level as MgCl ₂ . The bottom row shows that ZnCl ₂ can block citric acid up to around 0.1x – 0.5x concentration. Example 9 – metal effect on stability and sensitivity The effect of citric acid on colour changing compositions that contain different levels of Dodecyl- TMA (“D”) was investigated. For this example, the following solution composition was used:

*described in the general exemplary section above, here having a molar ratio Fe : CAS : A series of vials was prepared with the compositions above, with citric acid at various dilution levels as indicated in the tables below. In the tables below, the layout of cells (upper left to lower right) matches the layout of vials in Fig.14: Fig.14 – vial layout: Vials labelled with bold underlined text above had a blue colour (i.e. were not substantially affected) while the remaining vials were green. Below 0.1x dilution citric acid had no effect on the colour of the colour changing composition. Above 0.5x equivalents caused a colour change. This implies that the high level of ZnCl ₂ mitigates/blocks this effect. Example 10 – metal effect on stability and sensitivity Copper chloride amongst others has been reported in the literature to have a higher binding efficacy to EDTA than zinc chloride. Consequently, it was incorporated into the blocking composition and investigated for chelator inhibition at lower concentrations. For this example, the following solution composition was used: *described in the general exemplary section above, here having a molar ratio Fe : CAS : dodecyltrimethyl ammonium chloride of 1:2-3:0.5 - 3 (based on 2.50 * 10^-5 mol/L Fe in 200 μl). A series of vials was prepared with the composition above, with citric acid at 0.5x or 0.05x dilution levels as indicated in the tables below. In the tables below, the layout of cells (upper left to lower right) matches the layout of vials in Fig.15. Fig.15 – vial layout: Vials labelled with bold underlined text above had a blue colour (i.e. were not substantially affected) while the remaining vials were green (albeit 0.05x citrate dilution for the 0.001x and 0x vials had a blue tinge). The addition of CuCl ₂ caused the colour changing composition to become a much darker blue than the control (not shown) but prevent the interference of citrate with the dye complex, until there was less than 0.01 mol of CuCl ₂ compared to Fe. Example 11 – metal effect on stability and sensitivity For this example, the following solution composition was used: *described in the general exemplary section above, here having a molar ratio Fe : CAS : HDTMA of 1:2 - 3:0.5 - 3 (based on 2.50 * 10^-5 mol/L Fe in 400 μl). A series of vials was prepared with the composition above, with D10 or Dfx at various dilution levels as indicated in the tables below. Each dilution was conducted in pairs (i.e. each cell below represents a pair of vials), with a left hand (0 equivalents CaCl ₂ ) and a right hand (50 equivalents CaCl ₂ ) vial in each pair. Fig.16 – vial layout: All upper vials were teal coloured. The 0.4x Dfx vials were yellow, while the remainder were green-blue (with the deepest blues in the 0.1x and 0x control vials). The level of CaCl ₂ had limited effect on the presence of D10, except where D10 was at high levels (0.1x standard cleaning solution) where it prevented the solution turning yellow. The CaCl ₂ also had limited effect on the potential for the siderophore DFX to bind to the Fe centre of the colour changing composition. The experiment was repeated, with 100 (left hand vial in each pair) or 150 (right hand vial in each pair) equivalents of CaCl ₂ . The results are shown in Fig.17(A), which matches the vial layout in Fig.16, and Fig.17(B) which shows a specific comparison of the D10 vials at 0.06x and 0.05x against a MilliQ control and varying concentrations of calcium chloride. All upper vials were teal coloured. The 0.4x and 0.3x Dfx vials were yellow, while the remainder were green-blue (with the deepest blues in the 0.1x and 0x control vials). Here, the level of CaCl ₂ again had limited effect on the presence of D10, except where D10 was at high levels (0.2x standard cleaning solution), where it prevented the solution turning yellow. The CaCl ₂ also had limited effect on the potential for the siderophore DFX to bind to the Fe centre of the colour changing composition. Example 12 – metal effect on stability and sensitivity For this example, the following solution composition was used: *described in the general exemplary section above, here having a molar ratio Fe : CAS : surfactant of 1:2-3:0.5-3 (based on 0.0499 mol/mL Fe in 400 μl). A series of vials was prepared with the composition above, with D10 or Dfx at various dilution levels as indicated in the tables below. Each dilution was conducted in pairs (i.e. each cell below represents a pair of vials), with a left hand (of FCP) and a right hand (a comparator formulation of FCD) vial in each pair. Fig.18 – vial layout: All upper vials were teal coloured. The 0.4x and 0.3x Dfx vials were yellow, while the remainder were green-blue (with the deepest blues in the 0.1x and 0x control vials). Compared to the controls, FCD showed good stability to D10-mediated colour change, with sensitivity to siderophore (deferoxamine) at 0.2x equivalence to iron and above. FCD showed some colour change, but the colour change with Dfx occurred at a lower concentration and was more distinct. Example 13 – Interaction of FCD complex with D10 or Dfx with (Fig.19) or without (Fig. 20) a blocking composition For this example, the following solution composition was used:

*described in the general exemplary section above, here having a molar ratio Fe : CAS : surfactant of 1:2-3:0.5 - 3 (based on 0.0499 mol/mL Fe in 400 μl). A series of vials was prepared with the composition above with (Fig.19) or without (Fig.20) a blocking composition and tested against D10 or Dfx at various dilution levels as indicated in the tables below. Figs.19 and 20 – vial layout: All upper vials were blue coloured. The 0.3x and weaker Dfx vials were purple-blue, while the remainder were red. Fig.19 shows FCD not reacting with any level of D10 contamination when a blocking composition is used. At high concentrations (as typically seen in the food industry) of D10, FCD becomes discoloured and a brighter blue than the control. Fig.20 shows that the blocking composition still allows FCD to interact with a siderophore (deferoxamine) as the colour change become more intense as more Dfx is added (right to left). The effect of Dfx on colour is more apparent on the left than on the right, showing that the blocking composition prevents unwanted D10 interaction, whilst encouraging sensitivity to Dfx. Example 14 – metal effect on sensitivity For this example, the following solution composition was used: *described in the general exemplary section above, here having a molar ratio Fe : CAS : HDTMA of 1:2-3:0.5 - 3 (based on 0.0499 mol/mL Fe in 400 μl). A series of vials was prepared with the composition above with D10 or Dfx at various dilution levels as indicated in the tables below. Fig.21 – vial layout: All upper and middle vials were blue or teal coloured, with the deepest blues being at 1x D10 dilution. The 1x to 0.6x Dfx vials were yellow, 0.5x to 0.3x green-teal and 0.2x to 0.1x blue. The test was repeated with the same vial layout, but with 3 equivalents MgCl ₂ (relative to EDTA) instead of ZnCl ₂ and CaCl ₂ . The results are shown in Fig.22. The top two lines of both figures show a reducing level of D10 from left to right and top to bottom. The bottom line shows a reducing level of siderophore reducing in concentration. The blocking composition that includes MgCl ₂ (Fig.22) showed some discolouration with D10 from below 0.3x – 0.04x dilution compared to stock concentration. The effect of Dfx on the MgCl ₂ blocking composition is apparent from 0.1x and up. The blocking composition that includes the ZnCl ₂ and CaCl ₂ (Fig.20) showed a smaller sensitivity range to D10 (0.09x – 0.07x) and still had sensitivity to DFX from 0.2x and up. This shows a clear trade-off between metal ions that prevent D10 interference, and the effect of the metal ions on the sensitivity to DFX. Example 15 – metal effect on stability and sensitivity For this example, the following solution composition was used: *described in the general exemplary section above, here having a molar ratio Fe : CAS : HDTMA of 1:2-3:0.5-3 (based on 0.0499 mol/L Fe in 400 μl). A series of vials was prepared with the composition above with D10 or Dfx at various dilution levels as indicated in the tables below. Fig.23 – vial layout: All upper vials were blue with the exception of 0.3-0.1x, which progressively became more teal coloured. The middle row was teal. The 1x to 0.6x Dfx vials were yellow, 0.5x to 0.1x green- teal. The experiment was repeated, but with 50 equivalents CaCl ₂ (relative to EDTA) and no ZnCl ₂ . Fig.24 – vial layout: All upper vials were blue with the exception. The middle two rows were teal. The bottom row was yellow-teal, with deeper yellows towards the 1x side and deeper teal towards 0.1x. The range of sensitivity to D10 was increased compared to ZnCl ₂ at 50 equivalents, but the sensitivity to Dfx was increased. This further shows that a lower amount of ZnCl ₂ has a direct effect on sensitivity to both cleaning chemicals and bacterial residues.

Example 16 – the effect of detergent on colour change sensitivity The stability & sensitivity of the dye complex can be influenced by the amount of surfactant (e.g. HDTMA) that is present. For this example, the following solution was used: *described in the general exemplary section above, here having a molar ratio Fe : CAS : (C12-16) trimethyl ammonium chloride of 1:2 - 3:0 - 4 (based on 4.99 * 10^-5 mol/L Fe in 400 μl). An increased level of surfactant led to preservation of the peak from 600 – 710 nm, and reduced the increase of the peak from 450 – 460 nm when DFX was introduced. This is shown in Figure 25A (where the surfactant, “D”, had a ratio of 0 to Fe (solid line), where “D” had a ratio of 1 to Fe (dotted line), where “D” had a ratio of 2 to Fe (small dashes), where “D” had a ratio of 3 to Fe (long dashes) and where “D” had a ratio of 4 to Fe (dashed and dotted line)). Similarly, increasing the level of surfactant led to the preservation of the peak from 600 – 710 nm and the reduced the increase of the peak at 450 – 460 nm when D10 was introduced. This is shown in Figure 25B (where the surfactant, “D”, had a ratio of 0 to Fe (solid line), where “D” had a ratio of 1 to Fe (dotted line), where “D” had a ratio of 2 to Fe (small dashes), where “D” had a ratio of 3 to Fe (long dashes) and where “D” had a ratio of 4 to Fe (dashed and dotted line)). These graphs show that the level of surfactant is related to controlling both the sensitivity and stability of the colour-change composition. Example 17 Stock solutions of compositions were prepared by mixing the following solutions: Stock solution A: ∙ 50 ml of a solution comprising 0.06 g CAS in 50 ml H ₂ O; ∙ 9 ml of a solution comprising 0.0027 g hydrated FeCl ₃ (FeCl ₃ ⋅6H ₂ O) in 10 ml 10 mM HCl; ∙ 8 ml of a solution comprising 0.0146 g HDTMA in 8 ml H ₂ O; and ∙ 33 ml of a solution comprising 2 g Tween ^® 80 in 33 ml H ₂ O Stock solution B: ∙ 50 ml of a solution comprising 0.06 g CAS in 50 ml H ₂ O; ∙ 9 ml of a solution comprising 0.0081 g hydrated FeCl ₃ (FeCl ₃ ⋅6H ₂ O) in 10 ml 10 mM HCl; ∙ 8 ml of a solution comprising 0.0146 g HDTMA in 8 ml H ₂ O; and ∙ 33 ml of a solution comprising 2 g Tween ^® 80 in 33 ml H ₂ O 10 ml of each stock solution were diluted with 90 ml water to provide compositions for use as a cleaning spray. 10 ml of each stock solution were diluted with 10 ml of water to provide compositions for use in a label. Compositions may be added to the label by mixing with the carrier (e.g. agar) at a temperature of about 40 °C. Example 18 A range of tests were performed to investigate the colouring effect of increasing molar ratio of iron relative to CAS S (hydrated FeCl ₃ (FeCl ₃ ⋅6H ₂ O) to CAS S). The results were as follows: ∙ 1:0.0099 [1000x] – Blue ∙ 1:0.099 [100x] - Blue ∙ 1:0.485 [50x] - Blue ∙ 1:0.99 [10x] - Blue ∙ 1:9.9 [1x] – Pale Blue ∙ 1:19.8 [0.5x] - Red ∙ 1:99 [0.1x] - Red ∙ 1:198 [0.05x] - Red A deep blue colour was obtained with a ratio of 1:3.3. Example 19 Stock solution B was diluted by a factor of two to yield a solution for visibility testing. The solution was then made to the following dilutions, with the resulting qualitative visibility: ∙ 1x – dark blue, very little changes to orange seen with bacteria ∙ 2x – dark blue, still hard to see changes to orange with bacteria ∙ 5x – dark to medium blue, adding bacteria you can visibly see a grey and orange ∙ 10x – medium blue, mixing with bacteria shows a transparent orange colour ∙ 20x – near transparent blue, difficult to see orange colour because of high transparency ∙ 50x – almost completely clear with a hint blue Testing was performed in a 50 ml falcon tube with each dilution at a volume of 10 ml. 1 ml of OD 1 Bacteria (E-coli BL21 (DE3) cells and grown to an optical density of 1 as determined using UV- VIS) in water was added to each dilution to a final volume of 11 ml. The dilutions were placed at a volume of 1 ml on white weighing boats. Example 20 Membrane testing was performed with 10 kDa MWCO dialysis tubing. Dialysis tubing was cut to 10cm in length to form a cylinder of tubing open and both ends. After tying one end of the dialysis tubing, the formulation was added to 5ml total volume and then the second end was tied off to prevent any leaking of the formulation from the ends of the tubing. The membrane with the formulation was put into milk at room temperature for 24 hours. The results were compared with adding 5ml total volume directly to the milk and also compared relative to a control which comprised of the membrane filled with 5ml of water. No observable colour change occurred in the milk when the membrane was used. The formulation inside the membrane did change colour from blue to orange indicating that detection of siderophores was still possible. Example 21 An exemplary composition suitable for inclusion in a substrate, such as a surface wipe, is given below. 0.0008475 g of the composition was mixed with 12.25 mL water and then doped into a 10x10 cm cellulose wipe. Example 22 An experiment was conducted to demonstrate the effect of pH on the colour of compositions. A series of compositions having pH between 0.8 and 12.8 were prepared in accordance with the following procedure. Preparatory solutions with a pH between 0.8 and 6.8 were prepared from a stock solution comprising hydrochloric acid (1 mL, 37%) in distilled water (50 mL) and then diluted with sufficient further distilled water to yield solutions having a pH level 0.3 units lower than that intended for the final compositions for testing (e.g. where the final composition for testing was intended to have a pH of 1.8, then the preparatory solution was prepared by diluting the stock solution with further distilled water to a pH of 1.5). Preparatory solutions with a pH between 7.8 and 12.8 were prepared from a stock solution comprising sodium hydroxide (0.4 g) in distilled water (50 mL) and then diluted with further distilled water to yield solutions having a pH level 0.3 units higher than that intended for the final compositions for testing. Preparatory solutions were then diluted 1:1 (volume) with a water-mixed composition prepared in accordance with Example 21, to yield final compositions for testing having the desired pH. The (unbuffered) final compositions for testing were observed to have colouring as set out in the table below. A light absorbance study was conducted (Nanodrop 2000 Spectrophotometer, 0.1 mm path length) on the final compositions for testing to determine absorbance of CAS at 458 nm (λ _max absorption for CAS S) at variable pH between 1 and 13. The results are shown in Figure 26. Example 23 – validation of blocking effectiveness A method adapted from BS EN 1276:2019 was used to evaluate the blocking effectiveness of the blocking composition and any false negatives. A culture of Escherichia coli was produced and adjusted to 1.5-5.0x10 ⁸ CFU*/mL according to BS EN 1276:2019 to form a cell suspension. This organism was chosen due to its sensitivity to quaternary ammonium compounds. The cell suspension was then further diluted to achieve a cell count of 3.0x10 ² to 1.6x10 ³ CFU/mL to form a validation suspension. This validation suspension was then used in the blocking test. 100 µL of a commercial quaternary ammonium compound (QAC)-based disinfectant (Hycolin™ hospital disinfectant, diluted to 2% by volume) was added to 550 µL of blocking composition according to the present disclosure (including 150 µL of buffer) and incubated at ambient temperature for 5 minutes to allow blocking of the disinfectant. After blocking, 50 µL of the E. coli validation suspension was added to the tube and mixed thoroughly. Samples were incubated at ambient temperature for 5 minutes contact time, allowing exposure of the test organism to any residual un-blocked disinfectant. Samples were analysed to determine the number of surviving organisms in each tube. Control samples in which sterile distilled water was used in place of the disinfectant were also tested and results were compared. The counts obtained in the blocking effectiveness tests were assessed to confirm that all counts were ≥50% of the control count, indicating that the disinfectant had been blocked. Results are given in the table below. *CFU = colony-forming unit * * * The disclosure also comprises the following clauses, which may be claimed: 1. A substrate (optionally a sponge) comprising a blocking composition, wherein said blocking composition comprises: one or more metals; one or more blocking composition surfactants; and one or more pH buffers; wherein 0.15 ml of said composition comprises a combined amount of said metal and said blocking composition surfactant that is sufficient to block: at least around 1.372 x 10 ^-8 moles of n-alkyl dimethyl benzyl ammonium chloride; and at least around 3.106 x 10 ^-8 moles of C ₉ -C ₁₁ alkyl alcohol ethoxylate. 2. The substrate according to clause 1, wherein 0.15 ml of said composition comprises a combined amount of said metal and said blocking composition surfactant that is sufficient to block: at least around 2.854x10 ^-7 , optionally at least around 5.57x10 ^-7 , optionally at least around 8.287x10 ^-7 , optionally at least around 1.1x10 ^-6 , optionally around 1.3x10 ^-6 , such as around 1.372x10 ^-6 moles of n-alkyl dimethyl benzyl ammonium chloride; and at least around 6.46x10 ^-7 , optionally at least around 1.261x10 ^-6 , optionally at least around 1.876x10 ^-6 , optionally at least around 2.491x10 ^-6 , optionally around 3.1x10 ^-6 such as around 3.106x10 ^-6 moles of C ₉ -C ₁₁ alkyl alcohol ethoxylate. 3. The substrate according to clause 1 or 2, wherein 0.15 ml of said composition comprises a combined amount of said metal and said blocking composition surfactant that is sufficient to block: at most around 1.372 x 10 ^-4 , optionally at most around 1.1x10 ^-4 , optionally at most around 8.287x10 ^-5 , optionally at most around 5.57x10 ^-5 , optionally at most around 2.854x10- ⁵ , around 1.3x10 ^-6 , such as around 1.372x10 ^-6 moles of n-alkyl dimethyl benzyl ammonium chloride; and at most around 3.106 x 10 ^-4 , optionally at most around 2.491x10 ^-4 , optionally at most around 1.876x10 ^-4 , optionally at most around 1.261x10 ^-4 , optionally at most around 6.46x10- ⁵ , optionally around 3.1x10 ^-6 such as around 3.106x10 ^-6 moles of C ₉ -C ₁₁ alkyl alcohol ethoxylate. 4. The substrate according to any preceding clause, wherein 0.15 ml of said composition comprises a combined amount of said metal and said blocking composition surfactant that is sufficient to block: around 1.372x10 ^-8 to 1.372x10 ^-4 , optionally around 2.854x10 ^-7 to 1.1x10 ^-4 , optionally around 5.57x10 ^-7 to 8.287x10 ^-5 , optionally around 8.287x10 ^-7 to 5.57x10 ^-5 , optionally around 1.1x10 ^-6 to 2.854x10 ^-5 moles of n-alkyl dimethyl benzyl ammonium chloride; and around 3.106x10 ^-8 to 3.106x10 ^-4 , optionally around 6.46x10 ^-7 to 2.491x10 ^-4 , optionally around 1.261x10 ^-6 to 1.876x10 ^-4 , optionally around 1.876x10 ^-6 to 1.261x10 ^-4 , optionally around 2.491x10 ^-6 to 6.46x10 ^-5 moles of C ₉ -C ₁₁ alkyl alcohol ethoxylate. 5. The substrate according to any preceding clause, wherein 0.15 ml of said composition comprises a combined amount of said metal and said blocking composition surfactant that is sufficient to block said amount of n-alkyl dimethyl benzyl ammonium chloride and C ₉ -C ₁₁ alkyl alcohol ethoxylate, and at least: (a) around 1.937x 10 ^-8 moles of citrate, optionally at least around 4.029x10 ^-7 , optionally at least around 7.864x10 ^-7 , optionally at least around 1.17x10 ^-6 , optionally at least around 1.553x10 ^-6 , optionally around 1.9x10 ^-6 such as around 1.937x10 ^-6 ; and/or (b) around 2.959 x 10 ^-8 , optionally at least around 6.155x10 ^-7 , optionally at least around 1.201x10 ^-6 , optionally at least around 1.787x10 ^-6 , optionally at least around 2.373x10 ^-6 , optionally around 3x10 ^-6 such as around 2.959x10 ^-6 moles of ethylenediaminetetraacetic acid (EDTA). 6. The substrate according to any preceding clause, wherein 0.15 ml of said composition comprises a combined amount of said metal and said blocking composition surfactant that is sufficient to block said amount of n-alkyl dimethyl benzyl ammonium chloride and C ₉ -C ₁₁ alkyl alcohol ethoxylate, and at most: (a) around 1.937x10 ^-4 moles of citrate, optionally at most around 1.553x10 ^-4 , optionally at most around 1.17x10 ^-4 , optionally at most around 7.864x10 ^-5 , optionally at most around 4.029x10 ^-5 , optionally around 1.9x10 ^-6 such as around 1.937x10 ^-6 ; and/or (b) around 2.959x10 ^-4 moles of ethylenediaminetetraacetic acid (EDTA), optionally at most around 2.373x10 ^-4 , optionally at most around 1.787x10 ^-4 , optionally at most around 1.201x10 ^-4 , optionally at most around 6.155x10 ^-5 , optionally around 3x10 ^-6 such as around 2.959x10 ^-6 . 7. The substrate according to any preceding clause, wherein 0.15 ml of said composition comprises a combined amount of said metal and said blocking composition surfactant that is sufficient to block said amount of n-alkyl dimethyl benzyl ammonium chloride and C ₉ -C ₁₁ alkyl alcohol ethoxylate, and: (a) around 1.937 x 10 ^-8 to 1.937 x 10 ^-4 , optionally around 4.029x10 ^-7 to 1.553x10 ^-4 , optionally around 7.864x10 ^-7 to 1.17x10 ^-4 , optionally around 1.17x10 ^-6 to 7.864x10 ^-5 , optionally around 1.553x10 ^-6 to 4.029x10 ^-5 moles of citrate; and/or (b) around 2.959 x 10 ^-8 to 2.959 x 10 ^-4 , optionally around 6.155x10 ^-7 to 2.373x10 ^-4 , optionally around 1.201x10 ^-6 to 1.787x10 ^-4 , optionally around 1.787x10 ^-6 to 1.201x10 ^-4 , optionally around 2.373x10 ^-6 to 6.155x10 ^-5 moles of ethylenediaminetetraacetic acid (EDTA). 8. A substrate (optionally a sponge) comprising a blocking composition (optionally around 0.15 ml thereof), wherein said blocking composition comprises: one or more metals; one or more blocking composition surfactants; and one or more pH buffers, optionally wherein: around 0.15 ml of the blocking composition comprises a combined amount of said metal and said blocking composition surfactant that is sufficient to block: (a) at least around 1.937 x 10 ^-8 moles of citrate, optionally at least around 4.029x10- ⁷ , optionally at least around 7.864x10 ^-7 , optionally at least around 1.17x10 ^-6 , optionally at least around 1.553x10 ^-6 , optionally around 1.9x10 ^-6 such as around 1.937x10 ^-6 ; and/or (b) at least around 2.959 x 10 ^-8 moles of ethylenediaminetetraacetic acid (EDTA), optionally at least around 6.155x10 ^-7 , optionally at least around 1.201x10 ^-6 , optionally at least around 1.787x10 ^-6 , optionally at least around 2.373x10 ^-6 , optionally around 3x10 ^-6 such as around 2.959x10 ^-6 ; and/or (c) at most around 1.937 x 10 ^-8 moles of citrate, optionally at most around 1.553x10- ⁴ , optionally at most around 1.17x10 ^-4 , optionally at most around 7.864x10 ^-5 , optionally at most around 4.029x10 ^-5 , optionally around 1.9x10 ^-6 such as around 1.937x10 ^-6 ; and/or (d) at most around 1.937 x 10 ^-4 moles of ethylenediaminetetraacetic acid (EDTA), optionally at most around 2.373x10 ^-4 , optionally at most around 1.787x10 ^-4 , optionally at most around 1.201x10 ^-4 , optionally at most around 6.155x10 ^-5 , optionally around 3x10 ^-6 such as around 2.959x10 ^-6 ; and/or (e) around 1.937 x 10 ^-8 to 1.937 x 10 ^-4 moles of citrate, optionally around 4.029x10 ^-7 to 1.553x10 ^-4 , optionally around 7.864x10 ^-7 to 1.17x10 ^-4 , optionally around 1.17x10 ^-6 to 7.864x10 ^-5 , optionally around 1.553x10 ^-6 to 4.029x10 ^-5 ; and/or (f) around 2.959 x 10 ^-8 to 1.937 x 10 ^-4 moles of ethylenediaminetetraacetic acid (EDTA), optionally around 6.155x10 ^-7 to 2.373x10 ^-4 , optionally around 1.201x10 ^-6 to 1.787x10 ^-4 , optionally around 1.787x10 ^-6 to 1.201x10 ^-4 , optionally around 2.373x10 ^-6 to 6.155x10 ^-5 . 9. The substrate according to any preceding clause wherein the one or more metals comprise one or more metal salts selected from the group consisting of metal halides (optionally metal chlorides, metal fluorides, metal iodides and metal bromides), metal acetates, metal sulphates, metal carbonates, metal nitrates, metal phosphates, metal gluconates, metal oxides, metal hydroxides, metal citrates, metal lactates, metal glubionates, metal hydrates, metal peroxides, metal hypochlorides, metal dioxides and metal fumarates. 10. The substrate according to any preceding clause wherein the one or more metals comprise one or more metals, selected from the group consisting of zinc, calcium, magnesium, copper, bismuth, indium, manganese, nickel, titanium, chromium, aluminum, lithium, sodium, potassium, beryllium, radium, scandium, yttrium, lanthanum, vanadium, iron, cobalt, iridium, hafnium, silver, thallium, palladium, cadmium, tin, gallium, cerium, praseodymium, neodymium, promethium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium, americium, curium, berkelium, californium, thorium, uranium and neptunium, optionally from the group consisting of magnesium, calcium, zinc, copper, bismuth and manganese, or metal ions thereof, optionally from the group consisting of magnesium, calcium and zinc, or metal ions thereof. 11. The substrate according to any preceding clause, wherein the one or more metals comprise one or more metal ions selected from the group consisting of Zn ²⁺ , Ca ²⁺ , Mg ²⁺ , Cu ²⁺ , Cu ³⁺ ,

12. The substrate according to any preceding clause, wherein the one or more metals comprise zinc and/or calcium, optionally Zn ²⁺ and/or Ca ²⁺ , optionally ZnCl₂ and/or CaCl₂. 13. The substrate according to any preceding clause, wherein said blocking composition comprises around 0.0001 to 4 M of said one or more metals, optionally around 0.001 to 2 M; optionally 0.01 to 0.1 M, optionally around 0.05 to 0.25 M, optionally around 0.1 to 0.15 M. 14. The substrate according to any preceding clause, wherein the one or more metals comprise ZnCl ₂ and CaCl ₂ , optionally wherein said blocking composition comprises: around 0.00005 to 2 M of ZnCl ₂ , optionally around 0.001 to 1 M; optionally 0.005 to 0.05 M, optionally around 0.025 to 0.125 M, optionally around 0.05 to 0.1 M, optionally around 0.06 to 0.09 M, and around 0.00005 to 2 M of CaCl ₂ , optionally around 0.001 to 1 M; optionally 0.005 to 0.05 M, optionally around 0.025 to 0.125 M, optionally around 0.03 to 0.07 M, optionally around 0.04 to 0.06 M. 15. The substrate according to any preceding clause, wherein the one or more metals has a binding affinity, log K _f , for EDTA of at least around 4, optionally at least around 8, 10, 15, 20, 25, 30, 35, 40 or 45. 16. The substrate according to any preceding clause, wherein said metal has a binding affinity, log K _f, for EDTA of at most around 30, optionally at most around 25, 20, 15, 10 or 8. 17. The substrate according to any preceding clause, wherein said metal has a binding affinity, log K _f , for EDTA of around 4 to 45, optionally around 8 to 30, optionally around 10 to 30, optionally around 15 to 25, optionally around 20 to 25. 18. The substrate according to any preceding clause, wherein said metal has a binding affinity for Chromeazurol S of at most 15, optionally at most 13, optionally at most 10. 19. The substrate according to any preceding clause, wherein the one or more pH buffers are selected from the group consisting of: glycine, acetate, citrate, phosphate, ethanesulfonic acid and bis-tris methane. 20. The substrate according to any preceding clause, wherein the one or more pH buffers are selected from the group consisting of: glycine-hydrochloric acid, sodium acetate, piperazine- N,N′-bis(2-ethanesulfonic acid), citrate, phosphate, phosphate-citrate, 2-(N- morpholino)ethanesulfonic acid, 3-(N-morpholino)propanesulfonic acid and bis-tris methane. 21. The substrate according to any preceding clause, wherein the one or more pH buffers provide a pH working range of about 2.6 to 8, optionally 3.5 – 7, optionally around 5.6 – 7. 22. The substrate according to any preceding clause, wherein the one or more pH buffers provide a buffer capacity β of about -0.7 to 0.7 mol, optionally about -0.4 to 0.7 mol, optionally about -0.1 to 0.7 mol, optionally about 0.075 to 0.7 mol, optionally about 0.04 to 0.7 mol; and/or optionally about -0.7 to 0.4 mol, optionally about -0.7 to 0.1 mol, optionally about -0.7 to 0.04 mol, optionally about -0.7 to 0.01 mol; and/or optionally about -0.06 to 0.04, optionally about -0.05 to 0.02, optionally about -0.04 to 0.015 mol. 23. The substrate according to any preceding clause, wherein said blocking composition comprises around 10 mmol/L to 1 mol/L of said one or more pH buffers, optionally around 10 mmol/L to 750 mmol/L, optionally around 50 mmol/L to 500 mmol/L, optionally around 100 mmol/L to 300 mmol/L, optionally around 100 to 200 mmol/L, optionally around 130 to 170 mmol/L. 24. The substrate according to any preceding clause, wherein the one or more pH buffers comprise acetate, such as sodium acetate. 25. The substrate according to any preceding clause, wherein said blocking composition surfactant comprises a polysorbate (optionally a polysorbate 20, polysorbate 40, polysorbate 60, polysorbate 80 or optionally a Tween ^TM ); optionally at a level of around 1 mM to 200 mM; an aliphatic phenol ethoxylate (such as Triton X-100 ^TM ; optionally wherein the aliphatic group is an alkyl group; optionally wherein the aliphatic phenol ethoxylate is octyl phenol ethoxylate); optionally at a level of around 0.1 mM to 200 mM; a cyclodextrin (optionally β-cyclodextrin or γ-cyclodextrin); of a range between 1mM to 200mM; an aliphatic sulfate (optionally wherein the aliphatic group is an alkyl group, optionally wherein the aliphatic group has a straight chain length of 8 to 16 carbon atoms; optionally wherein the aliphatic sulfate is sodium dodecyl sulfate); optionally at a level of around 0.005 mM to 200 mM; a lecithin; optionally at a level of around 1 mM to 250 mM; or a pyruvate (optionally an alkyl pyruvate, sodium pyruvate or pyruvic acid, optionally wherein the alkyl pyruvate is methyl pyruvate) optionally at a level of around 1 mM to 10000 mM; a branched-chain or single-chain aliphatic carbonate (optionally wherein the aliphatic group is an alkyl group, optionally wherein the aliphatic group has a chain length of 8 to 22 carbon atoms, optionally 8 to 18 carbon atoms); a branched-chain or single-chain aliphatic benzene sulphonate (optionally wherein the aliphatic group is an alkyl group, optionally wherein the aliphatic group has a chain length of 8 to 16 carbon atoms, optionally 10 to 14 carbon atoms); a branched-chain or single-chain polyetheramine; or a cocamide diethanolamine (DEA) (optionally comprising a carbon chain having a chain length of 8 to 18 carbon atoms, optionally 8 to 12 carbon atoms). 26. The substrate according to any preceding clause, wherein said blocking composition surfactant comprises a polysorbate (optionally a polysorbate 20, polysorbate 40, polysorbate 60, polysorbate 80 or optionally a Tween ^TM ); optionally at a level of around 1 mM to 200 mM; an aliphatic phenol ethoxylate (such as Triton X-100 ^TM ; optionally wherein the aliphatic group is an alkyl group; optionally wherein the aliphatic phenol ethoxylate is octyl phenol ethoxylate); optionally at a level of around 0.1 mM to 200 mM; a cyclodextrin (optionally β-cyclodextrin or γ-cyclodextrin); of a range between 1mM to 200mM; an aliphatic sulfate (optionally wherein the aliphatic group is an alkyl group, optionally wherein the aliphatic group has a straight chain length of 8 to 16 carbon atoms; optionally wherein the aliphatic sulfate is sodium dodecyl sulfate); optionally at a level of around 0.005 mM to 200 mM; a lecithin; optionally at a level of around 1 mM to 250 mM; or a pyruvate (optionally an alkyl pyruvate, sodium pyruvate or pyruvic acid, optionally wherein the alkyl pyruvate is methyl pyruvate) optionally at a level of around 1 mM to 10000 mM. 27. The substrate according to any preceding clause, wherein said blocking composition surfactant comprises an aliphatic sulfate (optionally wherein the aliphatic group is an alkyl group, optionally wherein the aliphatic group has a chain length of 8 to 16 carbon atoms (optionally straight chain); optionally wherein the aliphatic sulfate is sodium dodecyl sulfate). 28. The substrate according to any preceding clause, wherein said blocking composition surfactant is as defined in any one of clauses 25 to 27 (optionally wherein said blocking composition surfactant is sodium dodecyl sulfate) present from an amount of about 1 x 10 ^-5 mol/L to about 3.5 x 10 ^-3 mol/L, optionally about 1 x 10 ^-4 mol/L to about 3.5 x 10 ^-3 mol/L, optionally about 2.059x10 ^-4 to 2.926x10 ^-3 , optionally about 3.119x10 ^-4 to 2.352x10 ^-3 , optionally about 4.178x10 ^-4 to 1.778x10 ^-3 , optionally about 5.238x10 ^-4 to 1.204x10 ^-3 . 29. The substrate according to any preceding clause, wherein said blocking composition further comprises 5-sulfosalicyclic acid. 30. The substrate according to any preceding clause, wherein said 5-sulfosalicyclic acid is present from an amount of about 1 x 10 ^-6 mol / L to about 5x10 ^-3 mol / L, optionally 1 x10 ^-5 mol / L to 2.5x10 ^-3 mol / L, optionally, 1x10 ^-4 mol / L to 1x10 ^-3 mol / L. 31. The substrate according to any preceding clause, wherein said substrate is an absorbent material, such as a sponge or wipe. 32. A composition comprising the blocking composition as defined in any preceding clause. 33. The composition according to clause 32, comprising: around 0.001 to 3 mol / L of one or more metals selected from the group consisting of: Zn ²⁺ , Ca ²⁺ , Mg ²⁺ , Cu ²⁺ , Cu ³⁺ , Bi ³⁺ , Mn ²⁺ , Mn ³⁺ , Ag ⁺ , Fe ²⁺ , Cr ²⁺ , Cr ³⁺ , Ce ⁴⁺ , Co ²⁺ , Bi ³⁺ , Ti ³⁺ , Al ³⁺ , Li ⁺ , K ⁺ , Na ⁺ and Be ²⁺ ; optionally wherein said one or more metals are selected from the group consisting of: Zn ²⁺ , Ca ²⁺ , Mg ²⁺ , Cu ²⁺ , Cu ³⁺ , Bi ³⁺ , Mn ²⁺ , Mn ³⁺ , Cr ²⁺ , Cr ³⁺ , Li ⁺ , K ⁺ , Na ⁺ , Be ²⁺ and Fe ²⁺ ; optionally wherein said one or more metals are selected from the group consisting of: Zn ²⁺ , Ca ²⁺ , Mg ²⁺ , Fe ²⁺ , Cr ³⁺ , Mn ²⁺ and Bi ³⁺ . 34. The composition according to clause 32 or 33, wherein the one or more metals are present as salts selected from the group consisting of: metal halides, metal acetates, metal carbonates and metal sulfates. 35. The composition according to any one of clauses 32 to 34, comprising around 10 to 750 mmol / L of buffer selected from the group consisting of: acetate, glycine, ethanesulfonic acid, bis-tris methane and phosphate. 36. The composition according to any one of clauses 32 to 35, comprising: (a) around 0.01 to 10 mmol / L of an aliphatic sulfate; or (b) around 1 to 100 mmol / L of an aliphatic phenol ethoxylate; or (c) around 10 to 200 mmol / L of a polysorbate. 37. The composition according to any one of clauses 32 to 36, comprising around 0.001 to 2 mol / L of the one or more metals, optionally around 0.001 to 1.5 mol / L, optionally around 0.001 to 1 mol / L. 38. The composition according to clause 32, comprising: around 0.001 to 3 mol / L of one or more metals selected from the group consisting of: Zn ²⁺ , Ca ²⁺ , Mg ²⁺ , Fe ²⁺ , Cr ³⁺ , Mn ²⁺ and Bi ³⁺ ; around 10 to 750 mmol / L buffer (optionally according to clause 35, optionally acetate buffer); around 0.01 to 10 mmol / L surfactant (optionally an aliphatic sulfate, optionally sodium dodecyl sulfate). 39. The composition according to clause 38, wherein the buffer is according to clause 35 (optionally acetate buffer). 40. The composition according to clause 38 or 39 (optionally according to clause 39), wherein the surfactant is an aliphatic sulfate (optionally sodium dodecyl sulfate). 41. The composition according to any one of clauses 32 to 40, wherein the one or more metals are Zn ²⁺ and Ca ²⁺ . 42. The composition according to any one of clauses 38 to 41 (optionally according to any one of clauses 39 to 41, optionally according to clause 40 or 41, optionally according to clause 41), comprising: around 0.001 to 1.5 mol / L of Zn ²⁺ ; around 0.001 to 1.5 mol / L of Ca ²⁺ . 43. The composition according to any one of clauses 32 to 42 (optionally according to any one of clauses 39 to 42), comprising around 10 to 750 mmol / L of acetate buffer. 44. The composition according to any one of clauses 32 to 43 (optionally according to any one of clauses 39 to 43), comprising around 0.01 to 10 mmol / L of an aliphatic sulfate. 45. The composition according to clause 41, comprising: around 0.001 to 1 mol / L of Zn ²⁺ ; around 0.001 to 1 mol / L of Ca ²⁺ ; around 12.5 to 400 mmol / L of acetate buffer; around 0.05 to 3.5 mmol / L sodium dodecyl sulfate. 46. The composition according to clause 45, comprising: around 15 to 250 mmol / L of Zn ²⁺ ; around 10 to 200 mmol / L of Ca ²⁺ ; around 12.5 to 400 mmol / L of acetate buffer; around 0.05 to 3.5 mmol / L sodium dodecyl sulfate. 47. The composition according to any one of clauses 32 to 46, comprising ZnCl ₂ and CaCl ₂ . 48. The composition of clause 32, wherein: said one or more metal comprises CaCl ₂ and ZnCl ₂ ; the composition comprises a sodium dodecyl sulfate blocking composition surfactant. 49. The composition according to clause 48, comprising: around 0.001 to 1.5 mol / L of ZnCl ₂ ; around 0.001 to 1.5 mol / L of CaCl ₂ ; around 10 to 750 mmol / L of acetate buffer; around 0.01 to 10 mmol / L sodium dodecyl sulfate. 50. The composition according to clause 49, comprising: around 0.001 to 1 mol / L of ZnCl ₂ ; around 0.001 to 1 mol / L of CaCl ₂ . 51. The composition according to clause 49 or 50, comprising around 12.5 to 400 mmol / L of acetate buffer. 52. The composition according to any one of clauses 49 to 51, comprising around 0.05 to 3.5 mmol / L sodium dodecyl sulfate. 53. The composition according to clause 49, comprising: around 12.5 to 400 mmol / L of acetate buffer; around 0.05 to 3.5 mmol / L sodium dodecyl sulfate. 54. The composition according to clause 49, comprising: around 0.001 to 1 mol / L of ZnCl ₂ ; around 0.001 to 1 mol / L of CaCl ₂ ; around 12.5 to 400 mmol / L of acetate buffer; around 0.05 to 3.5 mmol / L sodium dodecyl sulfate. 55. The composition according to clause 49, comprising: around 15 to 250 mmol / L of ZnCl ₂ ; around 10 to 200 mmol / L of CaCl ₂ ; around 12.5 to 400 mmol / L of acetate buffer; around 0.05 to 3.5 mmol / L sodium dodecyl sulfate. 56. A kit comprising: a substrate according to any one of clauses 1-31 and/or a composition according to any one of clauses 32-55; and (a) a colour changing composition, wherein said colour changing composition is configured to detect the presence of microorganisms; and/or (b) a cleaning composition, wherein said cleaning composition is configured to reduce and/or eliminate microorganisms. 57. The kit according to clause 56, wherein said substrate comprises around 0.05 ml to 0.75 ml of said blocking composition, optionally around 0.1 ml to 0.6 ml, optionally around 0.125 ml to 0.5 ml, optionally around 0.15 ml. 58. The kit according to clause 56 or 57, wherein said colour changing composition is present in an amount around 0.1 ml to 1 ml, optionally around 0.2 ml to 0.7 ml, optionally around 0.3 ml to 0.5 ml. 59. The kit according to any one of clauses 56 to 58, wherein said colour changing composition comprises: a colour changing composition metal, and a colour changing agent, wherein said colour changing composition metal is bindable to said colour changing agent to provide a change in colour on binding and/or release thereof. 60. The kit according to any one of clauses 56 to 59, further comprising a colour changing composition surfactant, optionally wherein said colour changing composition metal and colour changing composition surfactant are present at a molar ratio of about 1:0.25 to 30, optionally about 1:0.5 to 7; optionally about 1:0.75 to 6; optionally about 1:0.75 to 5; optionally about 1:0.75 to 3; optionally about 1:1 to 3; optionally about 1:1 to 2.5. 61. The kit according to clause 60, wherein said colour changing composition surfactant is selected from the group consisting of: a polysorbate (optionally a polysorbate 80 or polysorbate 20, optionally a Tween ^TM ), an aliphatic phenol ethoxylate (such as Triton X-100; optionally wherein the aliphatic group is an alkyl group; optionally wherein the aliphatic phenol ethoxylate is octyl phenol ethoxylate), an aliphatic sulfobetaine (optionally wherein the aliphatic group is an alkyl group, optionally wherein the aliphatic group has a chain length of 8 to 16 carbon atoms (optionally straight chain); optionally wherein the aliphatic sulfobetaine is lauryl sulfobetaine), an aliphatic quaternary ammonium halide such as aliphatic trimethylammonium halide (optionally wherein the aliphatic group is an alkyl group, optionally wherein the aliphatic group has a chain length of 8 to 22 carbon atoms (optionally straight chain), optionally wherein the aliphatic quaternary ammonium halide is either alkyl pyridinium halide or trimethylammonium halide, which can be myristyltrimethylammonium halide, trimethyloctadecylammonium halide, hexadecyl-trimethyl-ammonium bromide (HDTMA), 1-Dodecylpyridinium halide, or dodecyltrimethylammonium halide, optionally wherein the halide is a fluoride, chloride, bromide, or iodide), or combinations thereof, optionally wherein the aliphatic group has a chain length of 8 to 22 carbon atoms (optionally straight chain). 62. The kit according to any one of clauses 56 to 61, wherein said colour changing composition metal and colour changing agent are present at a molar ratio of about 1:0.25 to 25; optionally about 1:0.25 to 20, optionally 1:0.5 to 15; optionally about 1:1 to 13; optionally about 1:1.5 to 11.5; optionally about 1:1.5 to about 8; optionally about 1:1.5 to 5; optionally about 1:1.5 to 4. 63. The kit according to any one of clauses 56 to 62, wherein said colour changing composition metal is iron; optionally iron (III); optionally FeCl ₃ , optionally hydrated FeCl ₃ (FeCl ₃ ⋅6H ₂ O). 64. The kit according to any one of clauses 56 to 63, wherein said colour changing agent is a chromeazurol (such as chromeazurol S or chromeazurol B) or a tannin, optionally wherein said colour changing agent is a chromeazurol, optionally chromeazurol S (CAS). 65. The kit according to any one of clauses 56 to 64, wherein said cleaning composition comprises: an n-alkyl dialkyl triamine (e.g. wherein the n-alkyl is at least C ₁₀ , optionally C ₁₂ ; and/or optionally wherein the trialkyl is dipropylene, optionally wherein the n-alkyl dialkyl triamine is dodecyl dipropylene triamine), n-alkyl dimethyl ammonium halide (e.g. chloride), n-alkyl dialkyl aryl ammonium halide and/or aliphatic alcohol alkanoate, optionally wherein said n- alkyl dialkyl aryl ammonium halide is n-alkyl dimethyl benzyl ammonium halide, optionally n- alkyl dimethyl benzyl ammonium chloride, optionally wherein the n-alkyl is at least C ₈ (e.g. C ₈ -C ₁₀ ), optionally C ₁₂ -C ₁₆ n-alkyl dimethyl benzyl N-ammonium chloride; and/or wherein said aliphatic alcohol alkanoate is an alkyl alcohol alkanoate, optionally wherein the aliphatic group has a chain length of 8 to 18 carbon atoms (optionally straight chain), optionally wherein the aliphatic group has a chain length of 9 to 11 carbon atoms (optionally straight chain). 66. The kit according to clause 65, wherein said aliphatic alcohol alkanoate is an alkyl alcohol ethoxylate, optionally C ₈ -C18 alkyl alcohol ethoxylate, optionally C ₉ -C ₁₁ alkyl alcohol ethoxylate. 67. The kit according to any one of clauses 56 to 66, wherein said cleaning composition comprises n-alkyl dialkyl aryl ammonium halide present from about 0.5 wt% to 15 wt%; and aliphatic alcohol alkanoate present from about 0.5 wt% to 15 wt%. 68. The kit according to any one of clauses 56 to 67, wherein said cleaning composition comprises: n-alkyl dialkyl aryl ammonium halide present from about about 0.6 wt% to 14 wt%, optionally 0.7 wt% to 13 wt%, optionally 0.8 wt% to 12 wt%, optionally 0.9 wt% to 11 wt%, optionally about 1 wt% to 10 wt%; and aliphatic alcohol alkanoate present from about 0.6 wt% to 14 wt%, optionally 0.7 wt% to 13 wt%, optionally 0.8 wt% to 12 wt%, optionally 0.9 wt% to 11 wt%, optionally about 1 wt% to 10 wt% 69. The kit according to any one of clauses 56 to 68, wherein said cleaning composition further comprises citrate present from about 0.5 wt% to 10 wt%; optionally about 0.6 wt% to 9 wt%, optionally 0.7 wt% to 8 wt%, optionally 0.8 wt% to 7 wt%, optionally 0.9 wt% to 6 wt%, optionally about 1 wt% to 5 wt%. 70. The kit according to any one of clauses 56 to 69, wherein said cleaning composition further comprises EDTA present from about 0.5 wt% to 30 wt%; optionally about 0.6 wt% to 25 wt%, optionally 0.7 wt% to 20 wt%, optionally 0.8 wt% to 15 wt%, optionally 0.9 wt% to 12.5 wt%, optionally about 1 wt% to 10 wt%. 71. The kit according to any one of clauses 56 to 70, wherein said cleaning composition further comprises sodium hypochlorite present from about 1.8 wt% to 7 wt%, optionally 2.1 wt% to 6.5 wt%, optionally 2.4 wt% to 6 wt%, optionally 2.7 wt% to 5.5 wt%, optionally about 3 wt% to 5 wt%; and hydrogen peroxide present from about 6 wt% to 42 wt%, optionally 7 wt% to 39 wt%, optionally 8 wt% to 36 wt%, optionally 9 wt% to 33 wt%, optionally about 10 wt% to 30 wt%. 72. The kit according to any one of clauses 56 to 71, wherein said cleaning composition further comprises peracetic acid present from about 6 wt% to 42 wt%, optionally 7 wt% to 39 wt%, optionally 8 wt% to 36 wt%, optionally 9 wt% to 33 wt%, optionally about 10 wt% to 30 wt%; and acetic acid present from about 6 wt% to 42 wt%, optionally 7 wt% to 39 wt%, optionally 8 wt% to 36 wt%, optionally 9 wt% to 33 wt%, optionally about 10 wt% to 30 wt%. 73. A kit according to any one of clauses 56 to 72, wherein said blocking composition is as defined in any one of clauses 33 to 55; and wherein said colour changing composition comprises: a colour changing composition metal, wherein said colour changing composition is iron; a colour changing agent, wherein said colour changing agent is chromeazurol S; and optionally a colour changing composition surfactant, wherein said colour changing composition surfactant is an aliphatic quaternary ammonium halide; wherein said iron is bindable to chromeazurol S to provide a change in colour on binding and/or release thereof, optionally wherein said iron and said aliphatic quaternary ammonium halide are present at a molar ratio of 1:0.25 - 1:5; and wherein said iron and said chromeazurol S are present at a molar ratio of 1:0.5 - 1:5. 74. A kit according to any one of clauses 56 to 73, wherein said blocking composition is as defined in any one of clauses 33 to 55; and wherein said cleaning composition comprises: n-alkyl dialkyl aryl ammonium halide, wherein said n-alkyl dialkyl aryl ammonium halide is n-alkyl dimethyl benzyl ammonium chloride present at an amount from about 1 wt% to 10 wt%; and aliphatic alcohol alkanoate, wherein said aliphatic alcohol alkanoate is C ₉ -C ₁₁ alkyl alcohol ethoxylate present at an amount from about 1 wt% to 10 wt%, optionally wherein said cleaning composition further comprises citrate, optionally present at an amount from about 1 wt% to 5 wt% and/or EDTA, optionally present at an amount from about 1 wt% to 10 wt% optionally wherein said cleaning composition further compromises sodium hypochlorite, optionally present at an amount from about 3 wt% to 5 wt%; and/or hydrogen peroxide, optionally present at an amount from about 10 wt% to 30 wt%; and/or peracetic acid present at an amount from about 10 wt% to 30 wt%; and/or acetic acid present at an amount from about 10 wt% to 30 wt%. 75. A kit according to any one of clauses 56 to 74, wherein said blocking composition is as defined in any one of clauses 33 to 55; and wherein said colour changing composition is as defined in clause 73. 76. A swab comprising a rod having a substrate according to clauses 1-31 at an end thereof (optionally wherein the substrate comprises 0.15 ml of said composition), optionally further comprising a reservoir of a colour changing composition, optionally wherein said colour changing composition is as defined in any one of clauses 56 to 74, optionally wherein said reservoir is rupturable to enable release of said colour changing composition. 77. Use of a substrate according to any one of clauses 1 to 31, a blocking composition according to any one of clauses 32 to 55 or a swab according to clause 76 to block a cleaning composition, optionally wherein said cleaning composition is as defined in any one of clauses 56 to 74. 78. A method, comprising contacting a surface, or a sample therefrom, that has been pre- treated with a cleaning composition, with a blocking composition as defined in any one of clauses 32 to 55, optionally wherein said cleaning composition is as defined in any one of clauses 56 to 74. 79. The method according to clause 78, further comprising contacting said surface, or sample therefrom (e.g. collected by a swab optionally as defined in clause 74) with a colour changing composition, optionally wherein said colour changing composition is as defined in any one of clauses 56 to 74. 80. A substrate, composition, kit, swab, use or method substantially as described herein, with reference to the accompanying description above and figures. Any listing or discussion of an apparently prior-published document in this specification should not necessarily be taken as an acknowledgement that the document is part of the state of the art or common general knowledge. All references disclosed herein are to be considered to be incorporated herein by reference. Those skilled in the art will recognise or be able to ascertain using no more than routine experimentation many equivalents to the specific embodiments described herein. The scope of the present disclosure herein is not intended to be limited to the above description, but rather is as set forth in the appended claims. Those of ordinary skill in the art will appreciate that various changes and modifications to this description may be made without departing from the spirit or scope of the present disclosure.