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Title:
BLOOD COMPONENT MEASUREMENT METHOD UTILIZING HEMOLYZED WHOLE BLOOD, AND KIT FOR THE METHOD
Document Type and Number:
WIPO Patent Application WO/2010/010881
Kind Code:
A1
Abstract:
Disclosed is a method for determining the concentration of an analyte in the whole blood, wherein the analyte is a component which is contained in the blood, is different from a component occurring only in an erythrocyte, and can generate hydrogen peroxide upon the reaction with an oxidase, and wherein the whole blood is utilized in the method.  The method comprises the steps of: hemolyzing the whole blood; and detecting hydrogen peroxide generated by the reaction between the analyte and the oxidase.  The measurement method can avoid the inhibition of color development by hemoglobin and the interference with the measurement by hemoglobin, can be used for biological tests that are carried out in home or private clinics or at bedside of patients without the need of any hemocyte separation procedure or the like because the measurement utilizes the whole blood.

Inventors:
MACHIDA REIKO (JP)
IRIE YAYOI (JP)
YABUUTI MASAHIKO (JP)
UMEGAE YOSHIHIKO (JP)
Application Number:
PCT/JP2009/063077
Publication Date:
January 28, 2010
Filing Date:
July 22, 2009
Export Citation:
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Assignee:
NIPPON KAYAKU KK (JP)
MACHIDA REIKO (JP)
IRIE YAYOI (JP)
YABUUTI MASAHIKO (JP)
UMEGAE YOSHIHIKO (JP)
International Classes:
C12Q1/26; G01N33/50; G01N33/66; G01N33/70; G01N33/92; C12Q1/28; G01N33/52; G01N33/72
Domestic Patent References:
WO2008072702A12008-06-19
WO2003005039A12003-01-16
Foreign References:
JPH0870893A1996-03-19
JPH0870893A1996-03-19
JP2003521246A2003-07-15
JPH05304997A1993-11-19
JPS63185397A1988-07-30
JP2983015B21999-11-29
JP2001078797A2001-03-27
JP3170320B22001-05-28
JP3217180B22001-10-09
Other References:
See also references of EP 2319937A4
KANAI'S: "Manual of Clinical Laboratory Medicine", KANEHARA & CO., LTD
Attorney, Agent or Firm:
ASAMURA Kiyoshi et al. (JP)
Hiroshi Asamura (JP)
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