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Title:
BONE REGENERATION AGENT
Document Type and Number:
WIPO Patent Application WO/2015/064705
Kind Code:
A1
Abstract:
This bone regeneration agent, which has stem cells at a starting material and contains inactivated cell masses, the inactivated cell masses containing at least a calcium deposition and an extracellular matrix, has a bone conduction ability and a osteoinduction ability, can be produced at a low cost, and can be controlled in terms of size. Also, the bone regeneration agent can be easily produced by a method containing (1) a step for obtaining cell aggregates containing at least a calcium deposition and an extracellular matrix by inducing the differentiation of stem cells into calcium-deposition-producing cells in a shaking culture at 0.01-1.00 Hz, or (1') a step for obtaining cell aggregates containing at least a calcium deposition and an extracellular matrix by causing stem cells in which a protein participating in bone formation has been overexpressed by means of genetic manipulation to be subjected to a shaking culture or a static culture, inducing differentiation into calcium-deposition-producing cells, and (2) a step for inactivation processing of the cell aggregates obtained by means of the aforementioned step.

Inventors:
EGUSA HIROSHI (JP)
Application Number:
PCT/JP2014/078934
Publication Date:
May 07, 2015
Filing Date:
October 30, 2014
Export Citation:
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Assignee:
UNIV OSAKA (JP)
International Classes:
A61L27/00; A61K35/32; A61P19/00; C12N5/077; C12N5/0775; C12N5/10
Foreign References:
JP2004305259A2004-11-04
JP2011041472A2011-03-03
Other References:
JUN'ICHI SASAKI ET AL.: "Development of endochondral ossification analysis tool using 3D cell constructs", JOURNAL OF JAPANESE SOCIETY FOR DENTAL MATERIALS AND DEVICES, vol. 31, no. 2, 2012, pages 138
HIROKI KAYASHIMA ET AL.: "Ishoku ni Anzen na iPS Saibo Gijutsu o Kiban to shita Shinki Kotsu Zoseijutsu no Kaihatsu", JOURNAL OF JAPANESE SOCIETY OF ORAL IMPLANTOLOGY, vol. 26, August 2013 (2013-08-01)
HIROKI KAYASHIMA ET AL.: "Shinki Kotsu Ishoku Zairyo no Kaihatsu o Mezashita Shiniku Yurai iPS Saibo no Kotsuga Saibo Bunka Yudoho no Kakuritsu", JOURNAL OF JAPANESE SOCIETY OF ORAL IMPLANTOLOGY, vol. 24, 2011, pages 142
Attorney, Agent or Firm:
IWATANI, Ryo (JP)
Tatsu Iwatani (JP)
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