Title:
BORRELIDIN-PRODUCING POLYKETIDE SYNTHASE AND ITS USE
Document Type and Number:
WIPO Patent Application WO2004058976
Kind Code:
A3
Abstract:
The present invention relates to the biosynthesis of polyketides and derives from the cloning of nucleic acids encoding a polyketide synthase and other associated proteins involved in the synthesis of the polyketide borrelidin. Materials and methods including enzyme systems, nucleic acids, vectors and cells are provided for the preparation of polyketides including borrelidin and analogues and derivatives thereof. Novel polyketide molecules are also provided.
Inventors:
SALAS JOSE A (ES)
MENDEZ CARMEN (ES)
OLANO CARLOS (ES)
SANCHEZ CESAR (ES)
BRANA ALFREDO F (ES)
WILKINSON BARRIE (GB)
MARTIN CHRISTINE J (GB)
MOSS STEVEN (GB)
LEADLAY PETER F (GB)
OLIYNYK MARKO (GB)
MENDEZ CARMEN (ES)
OLANO CARLOS (ES)
SANCHEZ CESAR (ES)
BRANA ALFREDO F (ES)
WILKINSON BARRIE (GB)
MARTIN CHRISTINE J (GB)
MOSS STEVEN (GB)
LEADLAY PETER F (GB)
OLIYNYK MARKO (GB)
Application Number:
PCT/GB2003/005704
Publication Date:
February 17, 2005
Filing Date:
December 24, 2003
Export Citation:
Assignee:
BIOTICA TECH LTD (GB)
UNIV OVIEDO (ES)
SALAS JOSE A (ES)
MENDEZ CARMEN (ES)
OLANO CARLOS (ES)
SANCHEZ CESAR (ES)
BRANA ALFREDO F (ES)
WILKINSON BARRIE (GB)
MARTIN CHRISTINE J (GB)
MOSS STEVEN (GB)
LEADLAY PETER F (GB)
OLIYNYK MARKO (GB)
UNIV OVIEDO (ES)
SALAS JOSE A (ES)
MENDEZ CARMEN (ES)
OLANO CARLOS (ES)
SANCHEZ CESAR (ES)
BRANA ALFREDO F (ES)
WILKINSON BARRIE (GB)
MARTIN CHRISTINE J (GB)
MOSS STEVEN (GB)
LEADLAY PETER F (GB)
OLIYNYK MARKO (GB)
International Classes:
C07D313/00; C12N15/52; C12P17/08; (IPC1-7): C12N15/52; C07D313/00; C12P17/08; C12R1/465
Domestic Patent References:
| WO2001068867A1 | 2001-09-20 |
Foreign References:
| DE3607287A1 | 1988-01-07 |
Other References:
BENTLEY S D ET AL: "Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 417, no. 6885, 2002, pages 141 - 147, XP002233530, ISSN: 0028-0836
BANTLEON R ET AL: "CHLOROPEROXIDASE FROM STREPTOMYCES LIVIDANS: ISOLATION AND CHARACTERIZATION OF THE ENZYME AND THE CORRESPONDING GENE", JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 176, no. 8, 1 April 1994 (1994-04-01), pages 2339 - 2347, XP000560163, ISSN: 0021-9193
REEVES CHRISTOPHER D ET AL: "Alteration of the substrate specificity of a modular polyketide synthase acyltransferase domain through site-specific mutations", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 40, no. 51, 25 December 2001 (2001-12-25), pages 15464 - 15470, XP002194845, ISSN: 0006-2960
SWAN D G ET AL: "Characterisation of a Streptomyces antibioticus gene encoding a type I polyketide synthase which has an unusual coding sequence", MOLECULAR AND GENERAL GENETICS, SPRINGER VERLAG, BERLIN, DE, vol. 242, no. 3, 1994, pages 358 - 362, XP002087278, ISSN: 0026-8925
BANTLEON R ET AL: "CHLOROPEROXIDASE FROM STREPTOMYCES LIVIDANS: ISOLATION AND CHARACTERIZATION OF THE ENZYME AND THE CORRESPONDING GENE", JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 176, no. 8, 1 April 1994 (1994-04-01), pages 2339 - 2347, XP000560163, ISSN: 0021-9193
REEVES CHRISTOPHER D ET AL: "Alteration of the substrate specificity of a modular polyketide synthase acyltransferase domain through site-specific mutations", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 40, no. 51, 25 December 2001 (2001-12-25), pages 15464 - 15470, XP002194845, ISSN: 0006-2960
SWAN D G ET AL: "Characterisation of a Streptomyces antibioticus gene encoding a type I polyketide synthase which has an unusual coding sequence", MOLECULAR AND GENERAL GENETICS, SPRINGER VERLAG, BERLIN, DE, vol. 242, no. 3, 1994, pages 358 - 362, XP002087278, ISSN: 0026-8925
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