Title:
BROWN ADIPOCYTE DIFFERENTIATION-INDUCING AGENT
Document Type and Number:
WIPO Patent Application WO/2014/027608
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a means which is effective for the induction of the differentiation of a brown adipocyte and therefore enables a brown adipocyte to be used for and applied to, for example, the prevention/treatment of obesity or metabolic syndrome. Provided is a brown adipocyte differentiation-inducing agent containing, as an active ingredient, (1) CREG1 protein or (2) an expression vector carrying CREG1 gene.
Inventors:
YAMASHITA HITOSHI (JP)
KUSUDO TATSUYA (JP)
KUSUDO TATSUYA (JP)
Application Number:
PCT/JP2013/071591
Publication Date:
February 20, 2014
Filing Date:
August 09, 2013
Export Citation:
Assignee:
UNIV CHUBU EDUCATIONAL FOUNDATION (JP)
International Classes:
C12N15/09; A23L1/30; A23L1/305; A61K38/00; A61K48/00; A61P3/04; A61P3/06; A61P3/10; A61P9/10; C12N5/077; C12N5/0775; C12Q1/02; C12Q1/68
Other References:
VEAL E ET AL: "THE SECRETED GLYCOPROTEIN CREG ENHANCES DIFFERENTIATION OF NTERA-2 HUMAN EMBRYONAL CARCINOMA CELLS", ONCOGENE, vol. 19, 2000, pages 2120 - 2128, XP055197445
BACCO A D ET AL: "THE SECRETED GLYCOPROTEIN CREG INHIBITS CELL GROWTH DEPENDENT ON THE MANNOSE-6-PHOSPATE/INSULIN-LIKE GROWTH FACTOR II RECEPTOR", ONCOGENE, vol. 22, 2003, pages 5423 - 5445, XP055197446
SACHER M ET AL: "THE CRYSTAL STRUCTURE OF CREG, A SECRETED GLYCOPROTEIN INVOLVE GROWTH AND DIFFERENTIATION", PROC NATL ACAD SCI USA, vol. 102, no. 51, 2005, pages 18326 - 18331, XP055197451
HAN Y ET AL: "ADENOVITUS-MEDIATED INTRA ARTERIAL DELIVERY OF CELLULAR REPRESSOR OF EIA-STIMULATED GENES INHIBITS NEOINTIMA FORMATION IN RABBITS AFTER BALLOON INJURY", J VASCULAR SURGERY, vol. 48, 2008, pages 201 - 209, XP022760329
ZHANG Z ET AL: "SIGNAL PEPTIDE PREDICTION BASED ON ANALYSIS ON EXPERIMENTALLY VERIFIED CLEAVAGE SITES", PROTEIN SCI, vol. 13, 2004, pages 2819 - 2824, XP009051595
DATABASE GENBANK [online] NCBI; 21 April 2012 (2012-04-21), "HOMO SAPIENS CELLULAR REPRESSOR OF E1A GENES 1 (CREG1), MRNA", XP055197454, retrieved from NCBI Database accession no. NM_003851.2
NATURE, vol. 366, 1993, pages 740 - 742
NATURE, vol. 387, 1997, pages 90 - 94
AGING CELL, vol. 4, 2005, pages 147 - 155
N ENGL J MED, vol. 360, 2009, pages 1509 - 1517
"Science and Technology Trends", June 2009, SCIENCE AND TECHNOLOGY TRENDS RESERCH CENTER
CELL METAB, vol. 1, 2005, pages 361 - 370
NATURE, vol. 454, 2008, pages 961 - 967
VEAL E; EISENSTEIN M; TSENG ZH; GILL G., MOL CELL BIOL., vol. 18, no. 9, September 1998 (1998-09-01), pages 5032 - 41
DI BACCO, A.; GILL, G., ONCOGENE, 2003
SACHER, M.; DI BACCO, A.; LUNIN, V. V; YE, Z.; WAGNER, J.; GILL, G.; CYGLER, M., PROC. NATL. ACAD. SCI. U. S. A., 2005
KARLIN; ALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 2264 - 68
KARLIN; ALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873 - 77
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 10
ALTSCHUL ET AL., AMINO ACIDS RESEARCH, vol. 25, no. 17, 1997, pages 3389 - 3402
MYERS; MILLER, COMPUT APPL BIOSCI., vol. 4, 1988, pages 11 - 17
FELGNER, P. L.; GADEK, T. R.; HOLM, M. ET AL., PROC. NATL. ACAD. SCI., vol. 84, 1987, pages 7413 - 7417
DZAU, V. J.; MANN, M.; MORISHITA, R. ET AL., PROC. NATL. ACAD. SCI., vol. 93, 1996, pages 11421 - 11425
KANEDA, Y.; SAEKI, Y.; MORISHITA, R., MOLECULAR MED. TODAY, vol. 5, 1999, pages 298 - 303
MIYAKE, S.; MAKIMURA, M.; KANEGAE, Y.; HARADA, S.; TAKAMORI, K.; TOKUDA, C.; SAITO, 1., PROC. NATL. ACAD. SCI. USA, vol. 93, 1996, pages 1320
MIHO TERASHIMA; SAKI KONDO; YUMI KANEGAE; IZUMI SAITO, EXPERIMENTAL MEDICINE, vol. 21, no. 7, 2003, pages 931
GREEN AND SAMBROOK: "Molecular Cloning, Third Edition,", COLD SPRING HARBOR LABORATORY PRESS
FREDERICK M. AUSUBEL ET AL.,: "Current protocols in molecular biology", 1987
MARCH ET AL., HUMAN GENE THERAPY, vol. 6, 1995, pages 41 - 53
See also references of EP 2886650A4
BACCO A D ET AL: "THE SECRETED GLYCOPROTEIN CREG INHIBITS CELL GROWTH DEPENDENT ON THE MANNOSE-6-PHOSPATE/INSULIN-LIKE GROWTH FACTOR II RECEPTOR", ONCOGENE, vol. 22, 2003, pages 5423 - 5445, XP055197446
SACHER M ET AL: "THE CRYSTAL STRUCTURE OF CREG, A SECRETED GLYCOPROTEIN INVOLVE GROWTH AND DIFFERENTIATION", PROC NATL ACAD SCI USA, vol. 102, no. 51, 2005, pages 18326 - 18331, XP055197451
HAN Y ET AL: "ADENOVITUS-MEDIATED INTRA ARTERIAL DELIVERY OF CELLULAR REPRESSOR OF EIA-STIMULATED GENES INHIBITS NEOINTIMA FORMATION IN RABBITS AFTER BALLOON INJURY", J VASCULAR SURGERY, vol. 48, 2008, pages 201 - 209, XP022760329
ZHANG Z ET AL: "SIGNAL PEPTIDE PREDICTION BASED ON ANALYSIS ON EXPERIMENTALLY VERIFIED CLEAVAGE SITES", PROTEIN SCI, vol. 13, 2004, pages 2819 - 2824, XP009051595
DATABASE GENBANK [online] NCBI; 21 April 2012 (2012-04-21), "HOMO SAPIENS CELLULAR REPRESSOR OF E1A GENES 1 (CREG1), MRNA", XP055197454, retrieved from NCBI Database accession no. NM_003851.2
NATURE, vol. 366, 1993, pages 740 - 742
NATURE, vol. 387, 1997, pages 90 - 94
AGING CELL, vol. 4, 2005, pages 147 - 155
N ENGL J MED, vol. 360, 2009, pages 1509 - 1517
"Science and Technology Trends", June 2009, SCIENCE AND TECHNOLOGY TRENDS RESERCH CENTER
CELL METAB, vol. 1, 2005, pages 361 - 370
NATURE, vol. 454, 2008, pages 961 - 967
VEAL E; EISENSTEIN M; TSENG ZH; GILL G., MOL CELL BIOL., vol. 18, no. 9, September 1998 (1998-09-01), pages 5032 - 41
DI BACCO, A.; GILL, G., ONCOGENE, 2003
SACHER, M.; DI BACCO, A.; LUNIN, V. V; YE, Z.; WAGNER, J.; GILL, G.; CYGLER, M., PROC. NATL. ACAD. SCI. U. S. A., 2005
KARLIN; ALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 2264 - 68
KARLIN; ALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 5873 - 77
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 10
ALTSCHUL ET AL., AMINO ACIDS RESEARCH, vol. 25, no. 17, 1997, pages 3389 - 3402
MYERS; MILLER, COMPUT APPL BIOSCI., vol. 4, 1988, pages 11 - 17
FELGNER, P. L.; GADEK, T. R.; HOLM, M. ET AL., PROC. NATL. ACAD. SCI., vol. 84, 1987, pages 7413 - 7417
DZAU, V. J.; MANN, M.; MORISHITA, R. ET AL., PROC. NATL. ACAD. SCI., vol. 93, 1996, pages 11421 - 11425
KANEDA, Y.; SAEKI, Y.; MORISHITA, R., MOLECULAR MED. TODAY, vol. 5, 1999, pages 298 - 303
MIYAKE, S.; MAKIMURA, M.; KANEGAE, Y.; HARADA, S.; TAKAMORI, K.; TOKUDA, C.; SAITO, 1., PROC. NATL. ACAD. SCI. USA, vol. 93, 1996, pages 1320
MIHO TERASHIMA; SAKI KONDO; YUMI KANEGAE; IZUMI SAITO, EXPERIMENTAL MEDICINE, vol. 21, no. 7, 2003, pages 931
GREEN AND SAMBROOK: "Molecular Cloning, Third Edition,", COLD SPRING HARBOR LABORATORY PRESS
FREDERICK M. AUSUBEL ET AL.,: "Current protocols in molecular biology", 1987
MARCH ET AL., HUMAN GENE THERAPY, vol. 6, 1995, pages 41 - 53
See also references of EP 2886650A4
Attorney, Agent or Firm:
HAGINO Mikiharu (JP)
Kanji Hagino (JP)
Kanji Hagino (JP)
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