Title:
BUFFER SOLUTION FOR NUCLEIC ACID AMPLIFICATION COLORIMETRIC REACTION AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2020/098474
Kind Code:
A1
Abstract:
Disclosed in the present invention are a buffer solution for a nucleic acid amplification colorimetric reaction and application thereof. The buffer solution for a nucleic acid amplification colorimetric reaction in the present invention contains components having the following concentrations: a weak acidic substance: 0.5-10 mM and a divalent cationic salt: 0.5-10 mM. The buffer solution of the present invention is applied to preparation of a nucleic acid amplification colorimetric reaction system and/or nucleic acid amplification fluorescence detection. The nucleic acid amplification colorimetric reaction system of the present invention comprises one or more pairs of oligonucleotide primers for target nucleic acid amplification, one or more types of DNA polymerases, the buffer solution for the colorimetric reaction, and dNTPs.
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Inventors:
SHI CHAO (CN)
MA CUIPING (CN)
WANG XUEJIAO (CN)
JI YANLI (CN)
MA CUIPING (CN)
WANG XUEJIAO (CN)
JI YANLI (CN)
Application Number:
PCT/CN2019/113872
Publication Date:
May 22, 2020
Filing Date:
October 29, 2019
Export Citation:
Assignee:
QINGDAO NAVID BIOTECHNOLOGY CO LTD (CN)
International Classes:
C07H21/00; C12Q1/68; C07H21/04
Domestic Patent References:
| WO2017209920A1 | 2017-12-07 | |||
| WO2003043402A2 | 2003-05-30 |
Foreign References:
| US20150240293A1 | 2015-08-27 | |||
| CN103820541A | 2014-05-28 |
Other References:
TANNER NATHAN ET AL: "Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes", BIOTECHNIQUES, vol. 58, no. 2, 28 February 2015 (2015-02-28), pages 59 - 68, XP055245831, DOI: 10.2144/000114253
Attorney, Agent or Firm:
JEEKAI & PARTNERS (CN)
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