Title:
BULK CRYOPRESERVATION OF BIOLOGICAL MATERIALS AND BULK CRYOPRESERVATION OF BIOLOGICAL MATERIALS AND USES FOR CRYOPRESERVED AND ENCAPSULATED BIOLOGICALUSES FOR CRYOPRESERVED AND ENCAPSULATED BIOLOGICAL MATERIALS MATERIALS
Document Type and Number:
WIPO Patent Application WO1996021351
Kind Code:
A3
Abstract:
A method for bulk cryopreservation of biological material includes the steps of providing a flexible container, such as a freezer bag, containing biological material that is treated with a cryoprotectant and freezing the biological material to below -100 DEG C. and preferably below -196 DEG C. for deep freeze long-term storage. In the preferred embodiment, the bag is placed in a holder that maintains the cross-sectional area of the bag essentially constant and small enough (e.g., about 5 mm width to facilitate uniform heat transfer to and from all regions the bag. This facilitates uniform nucleation of the biological material after supercooling which enables controlled and uniform slow cooling through from about -7.5 DEG C. to a temperature in the range of about -40 DEG C. to -80 DEG C., thereby maintaining the viability of the cells. The single freezer bag method facilitates cryopreservation of large amounts of material, such as entire preparations of isolated islets, as compared to conventional multiple tube cryopreservation protocols and yields similar or improved recovery. In addition, the single freezer bag method reduces the risk of cross-contamination, a problem when adding and withdrawing material form multiple tubes before, during and after the cryopreservation freeze-thaw cycle. The bag construction also reduces the risk of freezing medium (e.g., liquid nitrogen) seeping into the bag and causing the bag to burst when thawing the material. The present invention is directed, in particular, to the cryopreservation of encapsulated islets in a flexible container wherein the container thickness during freezing is maintained around 5 mm. The freezing may also be accomplished by supercooling the encapsulated islets and inducing ice nucleation.
Inventors:
RAJOTTE RAY V (CA)
LAKEY JONATHAN R T (CA)
AO ZILIANG (CA)
COLBY C BUDD (CA)
FLASHNER MICHAEL (CA)
KORBUTT GREGORY S (CA)
WARNOCK GARTH L (CA)
LAKEY JONATHAN R T (CA)
AO ZILIANG (CA)
COLBY C BUDD (CA)
FLASHNER MICHAEL (CA)
KORBUTT GREGORY S (CA)
WARNOCK GARTH L (CA)
Application Number:
PCT/US1996/000640
Publication Date:
December 19, 1996
Filing Date:
January 16, 1996
Export Citation:
Assignee:
UNIV ALBERTA (CA)
RAJOTTE RAY V (CA)
LAKEY JONATHAN R T (CA)
AO ZILIANG (CA)
COLBY C BUDD (CA)
FLASHNER MICHAEL (CA)
KORBUTT GREGORY S (CA)
WARNOCK GARTH L (CA)
RAJOTTE RAY V (CA)
LAKEY JONATHAN R T (CA)
AO ZILIANG (CA)
COLBY C BUDD (CA)
FLASHNER MICHAEL (CA)
KORBUTT GREGORY S (CA)
WARNOCK GARTH L (CA)
International Classes:
A01N1/02; (IPC1-7): A01N1/02
Domestic Patent References:
| WO1991007951A1 | 1991-06-13 |
Foreign References:
| US4565073A | 1986-01-21 | |||
| US5071741A | 1991-12-10 | |||
| US4327799A | 1982-05-04 | |||
| US4117881A | 1978-10-03 | |||
| US4469227A | 1984-09-04 | |||
| EP0564880A1 | 1993-10-13 | |||
| US4194369A | 1980-03-25 | |||
| US5017338A | 1991-05-21 | |||
| US3943770A | 1976-03-16 |
Other References:
J.R.T. LAKEY ET AL.: "Development of a method for bulk cryopreservation of purified canine islets and human pancreatic microfragments", CELL TRANSPLANTATION, vol. 3, no. 3, 1994, pages 213, XP002017751
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; RAJOTTE R V ET AL: "PANCREATIC ISLET BANKING THE TRANSPLANTATION OF FROZEN THAWED RAT ISLETS TRANSPORTED BETWEEN CENTERS.", XP002017724
R.V. RAJOTTE ET AL.: "Viability Studies on Frozen-Thawed Rat Islets of Langerhans", CRYOBIOLOGY, vol. 14, no. 1, February 1977 (1977-02-01), NEW YORK, US, pages 116 - 120, XP000607861
DATABASE BIOSIS BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; RAJOTTE R V ET AL: "PANCREATIC ISLET BANKING THE TRANSPLANTATION OF FROZEN THAWED RAT ISLETS TRANSPORTED BETWEEN CENTERS.", XP002017724
R.V. RAJOTTE ET AL.: "Viability Studies on Frozen-Thawed Rat Islets of Langerhans", CRYOBIOLOGY, vol. 14, no. 1, February 1977 (1977-02-01), NEW YORK, US, pages 116 - 120, XP000607861
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