Title:
C4-DICARBOXYLIC ACID TRANSPORTER FOR INCREASING OIL YIELD OF MUCOR CIRCINELLOIDES
Document Type and Number:
WIPO Patent Application WO/2020/103854
Kind Code:
A1
Abstract:
Provided are a C4-dicarboxylic acid transporter for increasing the oil yield of mucor circinelloides and an encoding gene C4mt thereof. The C4mt gene is obtained from high-oil-yield mucor circinelloides WJ11 by means of cloning, and is transformed into a mucor circinelloides deficient strain Mu402; the c4mt gene is integrated to a mucor circinelloides genome by means of homologous recombination to obtain a recombinant strain Mu-C4mt; the total oil fatty acid content of the Mu-C4mt strain is improved by 25.30%, and the maximum intracellular oil content can reach 16.34% of the total fatty acids.
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Inventors:
SONG YUANDA (CN)
YANG JUNHUAN (CN)
YANG WU (CN)
YANG JUNHUAN (CN)
YANG WU (CN)
Application Number:
PCT/CN2019/119646
Publication Date:
May 28, 2020
Filing Date:
November 20, 2019
Export Citation:
Assignee:
UNIV SHANDONG TECHNOLOGY (CN)
International Classes:
C12N15/31; C07K14/37; C12N1/15; C12N15/80; C12R1/785
Foreign References:
CN109628473A | 2019-04-16 | |||
CN104926930A | 2015-09-23 | |||
US20140234925A1 | 2014-08-21 |
Other References:
LI, HONGQUAN ET AL.: "Progress of C4- dicarboxylate Transporter in Bacteria", JOURNAL OFHEBEI UNIVERSITY, vol. 27, no. 5, 25 September 2007 (2007-09-25), pages 555 - 558, ISSN: 1000-1565
YANG, L. ET AL.: "Overexpression of a C-4-dicarboxylate Transporter is the Key for Rerouting Citric Acid to C-4-dicarboxylic Acid Production in Aspergillus Carbonarius", MICROBIAL CELL FACTORIES, vol. 16, no. 43, 14 February 2017 (2017-02-14), pages 1 - 11, XP055710621, ISSN: 1475-2859, DOI: 10.1186/s12934-017-0660-6
YANG, L. ET AL.: "Overexpression of a C-4-dicarboxylate Transporter is the Key for Rerouting Citric Acid to C-4-dicarboxylic Acid Production in Aspergillus Carbonarius", MICROBIAL CELL FACTORIES, vol. 16, no. 43, 14 February 2017 (2017-02-14), pages 1 - 11, XP055710621, ISSN: 1475-2859, DOI: 10.1186/s12934-017-0660-6
Attorney, Agent or Firm:
FANG & ASSOCIATES (CN)
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