Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
CANCER CHEMOPROTECTIVE FOOD PRODUCTS
Document Type and Number:
WIPO Patent Application WO/1997/009889
Kind Code:
A1
Abstract:
Vegetable sources of cancer chemoprotective agents have been identified which are extraordinarily rich in glucosinolates, metabolic precursors of isothiocyanates. The vegetable sources are used to provide a dietary means of reducing the level of carcinogens in mammals.

Inventors:
FAHEY JED W (US)
TALALAY PAUL (US)
Application Number:
PCT/US1996/014866
Publication Date:
March 20, 1997
Filing Date:
September 13, 1996
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
HOPKINS J SCHOOL OF MEDICINE (US)
FAHEY JED W (US)
TALALAY PAUL (US)
International Classes:
A01H5/10; A21D2/36; A23L33/00; A21D13/00; A23L1/30; A23L2/38; A23L2/52; A23L7/152; A23L11/20; A23L19/00; A23L25/00; A61K31/00; A61K36/00; A61K36/18; A61K36/31; A61P35/00; (IPC1-7): A23L1/212; A61K35/78
Domestic Patent References:
WO1994019948A11994-09-15
Foreign References:
US4191752A1980-03-04
US3108040A1963-10-22
Other References:
R.MCDANELL ET AL.: "The effect of feeding brassica vegetables and intact glucosinolates on mixed-function-oxydase activity in the livers in intestines of rats", FOOD AND CHEMICAL TOXICOLOGY, vol. 27, no. 5, May 1989 (1989-05-01), GREAT BRITAIN, pages 289 - 293, XP000614081
S.GRAHAM ET AL.: "Diet in the epidemiology of cancer of the colon and rectum", JOURNAL OF THE NATIONAL CANCER INTITUTE, vol. 61, no. 3, - September 1978 (1978-09-01), WASHINGTON,D.C., pages 709 - 714, XP000614078
Download PDF:
Claims:
What Is Claimed Is:
1. Cruciferous sprouts, with the exception of Brassica oleracea capitata , Lepidium sativum, Sinapis alba, Sinapis niger, and Raphanus sativus sprouts, harvested prior to the 2leaf stage.
2. The cruciferous sprouts according to claim 1, wherein said sprouts are a Brassica oleracea selected from the group of varieties consisting of acephala, alboglabra, botrytis, costata, gemmifera, gongylodes, italica, medullosa, palmifolia, ramosa, sabauda, sabellica, and selensia .
3. The cruciferous sprouts according to claim 2, wherein said sprouts are a Brassica oleracea variety italica .
4. The cruciferous sprouts according to claim 1, wherein said sprouts are a Brassica oleracea variety botrytis .
5. The cruciferous sprouts according to claim 4, wherein said sprouts are a Brassica oleracea variety botrytis subvariety cauliflora .
6. The cruciferous sprouts according to claim 1, wherein said sprouts are substantially free of Phase 1 enzymeinducing potential.
7. A nontoxic solvent extract of the cruciferous sprouts according to claim 1.
8. The nontoxic solvent extract according to claim7 wherein said solvent is water.
9. The nontoxic solvent extract according to claim8 further comprising a cruciferous vegetable comprising an active myrosinase enzyme.
10. The nontoxic olvent extract according to claim 9, wherein said cruciferous vegetable is of the genus Raphanus.
11. A method of increasing the chemoprotective amount of Phase 2 enzymes in a mammal, comprising the step of administering an effective quantity of the cruciferous sprouts according to claim 1.
12. Cruciferous sprouts harvested prior to the 2 leaf stage, wherein said sprouts have at least 200,000 units per gram fresh wπight of Phase 2 enzymeinducing potential when measured after 3days of growth from seeds that produce said sprouts and nontoxic levels of indole glucosinolates and their breakdown products and goitrogenic hydroxybutenyl glucosinolates.
13. The cruciferous sprouts according to claim 12, wherein said sprouts are a Brassica oleracea selected from the group of varieties consisting of acephala, alboglabra, botrytis, costata, gemmifera, gongylodes, italica, medul losa , palmifolia, ramosa, sabauda, sabellica, and selensia .
14. The cruciferoi s sprouts according to claim 13, wherein said sprouts are a Brassica oleracea variety italica .
15. The cruciferous sprouts according to claim 13, wherein said sprouts ire a Brassica oleracea variety botrytis .
16. The cruciferous sprouts according to claim 15, wherein said sprouts are a Brassica oleracea variety botrytis subvariety caul iflora .
17. A nontoxic solvent extract of the cruciferous sprouts according to claim 12.
18. The nontoxic solvent extract according to claim17 wherein said solvent is water.
19. The nontoxic solvent extract according to claim18 further comprising a cruciferous vegetable comprising an active myrosinase enzyme.
20. The nontoxic solvent extract according to claim19 wherein said cruciferous vegetable is of the genus Raphanus .
21. A method of preparing a food product rich in glucosinolates, comprising germinating cruciferous seeds, with the exception of Brassica oleracea capitata , Lepidium sativum , Sinapis alba, Sinapis niger, and Raphanus sativus seeds, and harvesting sprouts prior to the 2leaf stage to form a food product comprising a plurality of sprouts.
22. The method according to claim 21, wherein said sprouts contain nontoxic levels of indole glucosinolates and their breakdown products and goitrogenic hydroxybutenyl glucosinolates.
23. The method according to claim 21, wherein said seeds are a Brassica oleracea selected from the group of varieties consisting of acephala, alboglabra, botrytis, costata, gemnifera, gongylodes, italica, medullosa, palmifolia, ramosa, sabauda, sabellica , and selensia .
24. The method according to claim 23, wherein said seeds are Brassica oleracea variety italica .
25. The method according to claim 23, wherein said seeds are Brassica oleracea variety botrytis .
26. The method according to claim 25, wherein said seeds are Brassica oleracea variety botrytis subvariety caul if lor a .
27. A food product rich in glucosinolates made by the process according to claim 21.
28. A method of preparing a food product, comprising extracting glucosinolates and isothiocyanates from cruciferous sprouts according to claim 1 with a nontoxic solvent, removing the extracted sprouts from said solvent, and recovering the extracted glucosinolates and isothiocyanates.
29. A method of preparing a food product according to claim 23, further comprising the step of drying said extracted glucosinolates and isothiocyanates.
30. A method of preparing a food product according to claim 28, wherein active myrosinase enzyme is mixed with said cruciferous sprouts, or said extracted glucosinolates and isothiocyanates, or both said cruciferous sprouts or said extract.
31. A method of preparing a food product rich in glucosinolates, comprising germinating cruciferous seeds that produce sprouts having at least 200,000 units per gram fresh weight of Phase 2 enzymeinducing potential when measured after 3days of growth and which contain nontoxic levels of indole glucosinolates and their breakdown products and goitrogenic hydroxybutenyl glucosinolates, and harvesting sprouts prior to the 2leaf stage to form a food product comprising a plurality of sprouts.
32. The method according to claim 30, wherein said seeds are a Brassica oleracea selected from the group of varieties consisting of acephala , alboglabra, botrytis, costata, gemmifera , ongy lodes , italica, medullosa, palmifolia , ramosa, sabauda , sabellica , and selensia .
33. The method according to claim 31, wherein said seeds are Brassica oleracea variety i talica .
34. The method according to claim 31, wherein said seeds are Brassica oleracea variety botrytis .
35. The method according to claim 33, wherein said seeds are Brassica oleracea variety botrytis subvariety cauliflora .
36. A food product rich in glucosinolates, made by the process according to claim 30.
37. A method of preparing a food product, comprising introducing cruciferous seeds, wherein said seeds produce sprouts having at least 200,000 units per gram fresh weight of Phase 2 enzymeinducing potential when measured after 3days of growth ana nontoxic levels of indole glucosinolates and their breakdown products and goitrogenic hydroxybutenyl glucosinolates, into another edible ingredient.
38. A method of preparing a food product, comprising extracting glucosinolates and isothiocyanates with a non toxic solvent and isothiocyanantes from cruciferous seeds, sprouts, plants or plant parts wherein seeds that produce said sprouts, plant, or plant parts, have at least 200,000 units per gram fresh weight of Phase 2 enzymeinducing potential when measured after 3days of growth and wherein said seeds, sprouts, plants or plant parts have nontoxic levels of indole glucosinolates and their breakdown products and goitrogenic hydroxybutenyl glucosinolates, and recovering the extracted glucosinolates and isothiocyanates.
39. A method of preparing a food product according to claim 37, wherein active myrosinase enzyme is mixed with said cruciferous seeds, sprouts or plants; or said extracted glucosinolates and isothiocyanates; or both said cruciferous seeds, sprouts or plants and said extract.
40. A method of reducing the level of carcinogens in a mammal, comprising administering to a mammal an effective amount of cruciferous sprouts, with the exception of Brassica oleracea capitata , Lepidium sativum , Sinapis alba and Sinapis niger, and Raphanus sativus sprouts.
41. A method of reducing the level of carcinogens in a mammal, comprising administering to a mammal an effective amount of cruciferous sprouts having at least 200,000 units per gram fresh weight of Phase 2 enzyme inducing potential when measured after 3days of growth from seeds that produce said sprouts and nontoxic levels of indole glucosinolates and their breakdown products and goitrogenic hydroxybutenyl glucosinolates.
42. A method of extracting glucosinolates and isothiocyanates from plant tissue comprising the steps of homogenizing said plant tissue in an excess of a mixture of dimethyl sulfoxide, acetonitrile and dimethylformamide at a temperature sufficient to inactivate myrosinase enzyme activity.
43. A food product comprising cruciferous sprouts, with the exception of Brassica oleracea capitata, Lepidium sativum, Sinapis alba, Sinapis niger, and Raphanus sativus sprouts, harvested prior to the 2leaf stage, cruciferous seeds; extracts of said sprouts or seeds; or any combination of said sprouts, seeds or extracts.
44. A method of increasing the chemoprotective amount of Phase 2 enzymes in a mammal, comprising the step of administering an effective quantity of the food product according to claim 42.
45. A food product comprising cruciferous sprouts harvested prior to the 2leaf stage, wherein said sprouts have at least 200,000 units per gram fresh weight of Phase 2 enzymeinducing potential when measured after 3 days of growth from seeds that produce said sprouts and nontoxic levels of indole glucosinolate and their breakdown products and goitrogenic hydroxybutenyl glucosinolates; cruciferous seeds; extracts of said sprouts or seeds; or any combination of said sprouts, seeds or extracts.
46. A method of increasing the chemoprotective amount of Phase 2 enzymes in a mammal, comprising the step of administering an effective quantity of the food product according to claim 44.
47. Cruciferous sprouts harvested prior to the 2 leaf stage, wherein the ratio of monofunctional to bifunctional inducers is at least 20 to 1.
48. A food product supplemented with a purified or partially purified glucosinolate.