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Title:
CAR-T CELL FOR EFFICIENTLY AND STABLY EXPRESSING INHIBITING ANTIBODY AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2017/219937
Kind Code:
A1
Abstract:
A CAR-T cell for efficiently and stably expressing an inhibiting antibody and an application thereof, and specifically, a transgenic T cell. A genome of the T cell stably integrates expression cassettes comprising nucleotide sequences of an encoded chimeric antigen receptor and an immune checkpoint inhibiting antibody, and two ends of the expression cassette comprise an inverted terminal repeat sequence of transposons. The CAR-T cell genome stably integrates the expression cassette of the inhibiting antibody by using a transposon system, so as to efficiently and stably express the activity of the inhibiting antibody while keeping the killing activity of the original T cell. An infused CAR-T cell is prevented from being inhibited by a tumor microenvironment, and in-situ activation of a residual tumor specific T cell can be implemented. In addition, to ensure the treatment safety of the pluripotent CAR-T cell, a molecular brake system is introduced, and the infused pluripotent CAR-T cell may be cleared in time if necessary.

Inventors:
QIAN QIJUN (CN)
JIN HUAJUN (CN)
XU JIEYING (CN)
LI LINFANG (CN)
YE ZHENLONG (CN)
HE ZHOU (CN)
JIANG DUQING (CN)
WU HONGPING (CN)
Application Number:
PCT/CN2017/088960
Publication Date:
December 28, 2017
Filing Date:
June 19, 2017
Export Citation:
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Assignee:
SHANGHAI CELL THERAPY RES INST (CN)
SHANGHAI ENGINEERING RES CENTER FOR CELL THERAPY GROUP CO LTD (CN)
International Classes:
C12N5/10; A61K35/17; A61K39/395; A61P35/00
Domestic Patent References:
WO2014134165A12014-09-04
WO2015164594A12015-10-29
Foreign References:
CN103965361A2014-08-06
CN105154473A2015-12-16
CN105331586A2016-02-17
CN105481984A2016-04-13
CN105330750A2016-02-17
CN103483453A2014-01-01
Other References:
"Ningbo No 5 Hospital", 24 October 2016 (2016-10-24), Retrieved from the Internet
Attorney, Agent or Firm:
SHANGHAI PATENT & TRADEMARK LAW OFFICE, LLC (CN)
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