received by the International Bureau on 24.08.2017

We claim:

1. A cartridge (100) for purifying a sample and detection of multiple analytes, the cartridge (100) comprising:

a first chamber (10) having a plurality of compartments (18) for loading the sample and at least one reagent, wherein the first chamber (10) is configured to mix the sample with the at least one reagent;

a second chamber (20) in fluid communication with the first chamber (10) .the second chamber (20)is configured with a matrix member (22), wherein the matrix member (22) receives a mixture of the sample and the at least one reagent, and is adapted to selecti vely bind at least one analyte from the mixture;

a third chamber (30) in fluid communicaiion with the second chamber (20), the third chamber defines a waste collection chamber (30a) for storing waste fluids discharged from the second chamber (20); and

a fourth chamber (40) in fluid communication with the third chamber (30), includes at least one tube (42) configured to receive the at least one analyte from the second chamber (20), through the third chamber (30),

2. wherein the second chamber (20) is mounted below the first: chamber

(10), the third chamber (30) mounted below the second chamber (20) and the fourth chamber (40) is mounted below the third chamber

(30), The cartridge (100) as claimed in claim 1, wherein the plurality of compartments (18) includes a sample chamber (18a) for loading the sample and at least one reactant chamber ( 18b) for loading the at least one reactant.

3. The cartridge (100) as claimed in claim 1 , wherein the first chamber (10) comprises a reaction chamber (18c) in fluid communication with the plurality of compartments (18) for mixing the sample with the at least one reagent.

4. The cartridge (100) as claimed in claim 1, wherein the first chamber (10) includes a heating element (16) for heating the sample and the at least one reagent.

5. The cartridge (100) as claimed in claim 1, wherein the matrix member (22) is a cotton matrix.

6. The cartridge (100) as claimed in claim 1, wherein the sample is at least one of biological samples and non-biological samples.

7. The cartridge (100) as claimed in claim 1, wherein the third chamber (30) includes a flow path (30b) in fluid communication with the second chamber (20) and the at least one tube (42) for routing the at least one analyte.

8. The cartridge (100) as claimed in claim 1, wherein the mixture of sample, the at least one reactant, the waste fluids and the at least one analyte flow within the cartridge (100) by gravity,

9. The cartridge (100) as claimed in claim 1, wherein the mixture of sample, the at least one reactant, the waste fluids and the at least one analyte flow within the cartridge (100) by externally applied pressure.