Title:
CEA.CAR-T, PREPARATION METHOD THEREFOR AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2018/205344
Kind Code:
A1
Abstract:
The present invention belongs to the field of biotechnology, and provides a CEA.CAR-T, a preparation method therefor and use thereof. The present invention firstly provides a chimeric antigen receptor targeting a carcinoembryonic antigen, the chimeric antigen receptor comprising polypeptides binding to carcinoembryonic antigen (CEA), CD28 transmembrane domain (CD28-TM), intracellular 4-1BB and intracellular CD3ζ, which are linked in sequence. Then, T cells that can express said chimeric antigen receptor are prepared.
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Inventors:
JIANG JINGTING (CN)
LU BINFENG (CN)
CHEN YU'ANG (CN)
CHEN LUJUN (CN)
LU BINFENG (CN)
CHEN YU'ANG (CN)
CHEN LUJUN (CN)
Application Number:
PCT/CN2017/088507
Publication Date:
November 15, 2018
Filing Date:
June 15, 2017
Export Citation:
Assignee:
THE FIRST PEOPLE S HOSPITAL OF CHANGZHOU (CN)
International Classes:
C07K19/00; A61K35/17; A61P35/00; C12N5/10; C12N15/62
Domestic Patent References:
| WO2016168493A1 | 2016-10-20 |
Foreign References:
| CN104910279A | 2015-09-16 | |||
| CN101370827A | 2009-02-18 | |||
| US9573988B2 | 2017-02-21 | |||
| CN106366178A | 2017-02-01 |
Other References:
HOMBACH, A. ET AL.: "Tumor-specific T cell activation by recombinant immunorecepto-rs: CD 3 zeta signaling and CD 28 costimulation are simultaneously required for efficient IL -2 secretion and can be integrated into one combined CD 28/ CD 3 zeta signaling receptor molecule", JOURNAL OF IMMUNOLOGY, vol. 167, no. 11, 1 December 2001 (2001-12-01), XP055083123, DOI: doi:10.4049/jimmunol.167.11.6123
SCHLIMPER, C. ET AL.: "Improved Activation toward Primary Colorectal Cancer Cells by Antigen-Specific Targeting Autologous Cytokine-Induced Killer Cells", CLINICAL AND DEVELOPMENTAL IMMUNOLOGY, 19 March 2012 (2012-03-19)
YU , XIAOLING ET AL.: "Lentiviral Vector of Chimeric Antigen Receptor Targeting Carcinoembryonic Antigen and Its Application", JOURNAL OF THIRD MILITARY MEDICAL UNIVERSITY, vol. 37, no. 11, 15 June 2015 (2015-06-15)
YU , XIAOLING: "Construction and Evaluation of Carcinoembryonic Antigen-Specific Chimeric Receptor", CHINA MASTER'S THESES DATABASE, 15 March 2016 (2016-03-15)
DATABASE GenBank 26 July 2016 (2016-07-26), NEUMAIER, M. ET AL.: "Anti-CEAmAb T84.66, partial [Mus musculus", Database accession no. CM36979. 1
DATABASE GenBank 26 July 2016 (2016-07-26), NEUMAIER, M. ET AL.: "Anti-CEAmAb T84.66, partial [Mus musculus", Database accession no. CM36980. 1
SCHLIMPER, C. ET AL.: "Improved Activation toward Primary Colorectal Cancer Cells by Antigen-Specific Targeting Autologous Cytokine-Induced Killer Cells", CLINICAL AND DEVELOPMENTAL IMMUNOLOGY, 19 March 2012 (2012-03-19)
YU , XIAOLING ET AL.: "Lentiviral Vector of Chimeric Antigen Receptor Targeting Carcinoembryonic Antigen and Its Application", JOURNAL OF THIRD MILITARY MEDICAL UNIVERSITY, vol. 37, no. 11, 15 June 2015 (2015-06-15)
YU , XIAOLING: "Construction and Evaluation of Carcinoembryonic Antigen-Specific Chimeric Receptor", CHINA MASTER'S THESES DATABASE, 15 March 2016 (2016-03-15)
DATABASE GenBank 26 July 2016 (2016-07-26), NEUMAIER, M. ET AL.: "Anti-CEAmAb T84.66, partial [Mus musculus", Database accession no. CM36979. 1
DATABASE GenBank 26 July 2016 (2016-07-26), NEUMAIER, M. ET AL.: "Anti-CEAmAb T84.66, partial [Mus musculus", Database accession no. CM36980. 1
Attorney, Agent or Firm:
J.Z.M.C.PATENT AND TRADEMARK LAW OFFICE (CN)
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