Title:
CELL AGGREGATION DEVICE, HOLDER FOR USE IN SAME, CELL AGGREGATION METHOD, BIOLOGICAL TISSUE FORMATION METHOD, LAYERED CELL SHEET, AND PRODUCTION METHOD THEREFOR
Document Type and Number:
WIPO Patent Application WO/2013/129681
Kind Code:
A1
Abstract:
A suitable cell within a well (7) is selected by means of an imaging device (21), and the well (7) having the suitable cell (13) therein is irradiated with a laser from a laser source (17) on the basis of information regarding the selection. Using the laser source (17), the laser is irradiated from above a transparent plate (9) so that the laser traverses the transparent plate (9) and irradiates a light-absorbing member (5). The laser beam is absorbed by the light-absorbing member (5) and generates heat, which heats the culture medium (23) within the well (7) and generates micro-bubbles. The generation of micro-bubbles within the well (7) generates pressure which ejects the cell (13) (and the culture medium) on the interior of the well (7) in the direction towards a table (15). In this manner, a cell at a given location can be extracted onto the table (15) or a culture bed (16).
Inventors:
KOUMURA YUKIO (JP)
Application Number:
PCT/JP2013/055799
Publication Date:
September 06, 2013
Filing Date:
March 04, 2013
Export Citation:
Assignee:
FURUKAWA ELECTRIC ADVANCED ENGINEERING CO LTD (JP)
International Classes:
C12M1/00; C12N1/00
Foreign References:
| JP2010022251A | 2010-02-04 |
Other References:
MASATO NAKAMURA: "Saibo kara Soshiki to Zoki o Tsukuru Kogaku Gijutsu -Bioprinting, Biofabrication", EXPERIMENTAL MEDICINE, vol. 28, no. 2, 2010, pages 185 - 192, XP008174198
HENMI C. ET AL.: "Development of an effective three dimensional fabrication technique using inkjet technology for tissue model samples.", PROC. 6TH WORLD CONGRESS ON ALTERNATIVES & ANIMAL USE IN THE LIFE SCIENCES, 2007, pages 689 - 692
GUILLOTIN B. ET AL.: "Laser assisted bioprinting of engineered tissue with high cell density and microscale organization.", BIOMATERIALS, vol. 31, 2010, pages 7250 - 7256, XP027182459
MÉZEL C. ET AL.: "Bioprinting by laser-induced forward transfer for tissue engineering applications: jet formation modeling.", BIOFABRICATION, vol. 2, 2010, pages 1 - 7
BINDER K. W. ET AL.: "DROP-ON-DEMAND INKJET BIOPRINTING: A PRIMER.", GENE THERAPY AND REGULATION, vol. 6, no. 1, 2011, pages 33 - 49, XP055131119, DOI: doi:10.1142/S1568558611000258
HENMI C. ET AL.: "Development of an effective three dimensional fabrication technique using inkjet technology for tissue model samples.", PROC. 6TH WORLD CONGRESS ON ALTERNATIVES & ANIMAL USE IN THE LIFE SCIENCES, 2007, pages 689 - 692
GUILLOTIN B. ET AL.: "Laser assisted bioprinting of engineered tissue with high cell density and microscale organization.", BIOMATERIALS, vol. 31, 2010, pages 7250 - 7256, XP027182459
MÉZEL C. ET AL.: "Bioprinting by laser-induced forward transfer for tissue engineering applications: jet formation modeling.", BIOFABRICATION, vol. 2, 2010, pages 1 - 7
BINDER K. W. ET AL.: "DROP-ON-DEMAND INKJET BIOPRINTING: A PRIMER.", GENE THERAPY AND REGULATION, vol. 6, no. 1, 2011, pages 33 - 49, XP055131119, DOI: doi:10.1142/S1568558611000258
Attorney, Agent or Firm:
INOUE, SEIICHI (JP)
Seiichi Inoue (JP)
Seiichi Inoue (JP)
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Claims: