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Title:
CELL ANALYSIS METHOD, CELL ANALYSIS DEVICE, AND CELL ANALYSIS PROGRAM
Document Type and Number:
WIPO Patent Application WO/2012/147403
Kind Code:
A1
Abstract:
Provided is a cell analysis method in a cell analysis device (D) that uses an optical path length image of a cell colony constituted of a large number of cells to analyze the cell colony, comprising: a step wherein an acquisition unit (D1) of the cell analysis device (D) acquires an optical path length image of a cell colony; a step wherein an extraction unit (D2) of the cell analysis device (D) extracts, from the acquired optical path length image, circular shapes each corresponding to a cell nucleus of a cell; a step wherein a comparison unit (D3) of the cell analysis device (D) compares, for each extracted circular shape, an inner optical path length and an outer optical path length thereof; and a step wherein an analysis unit (D4) of the cell analysis device (D) analyzes the cell colony on the basis of the comparison result.

Inventors:
SUGIYAMA NORIKAZU (JP)
TAKESHIMA TOMOCHIKA (JP)
KANEKO KOUICHI (JP)
Application Number:
PCT/JP2012/054457
Publication Date:
November 01, 2012
Filing Date:
February 23, 2012
Export Citation:
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Assignee:
HAMAMATSU PHOTONICS KK (JP)
SUGIYAMA NORIKAZU (JP)
TAKESHIMA TOMOCHIKA (JP)
KANEKO KOUICHI (JP)
International Classes:
C12M1/34; C12Q1/02; G01N21/17
Foreign References:
JP2009542237A2009-12-03
JP2011024485A2011-02-10
JP2008212017A2008-09-18
JP2003052974A2003-02-25
Other References:
LUE, NIYOM ET AL.: "Synthetic aperture tomographic phase microscopy for 3D imaging of live cells in translational motion.", OPTICS EXPRESS, vol. 16, no. 20, 2008, pages 16240 - 16246, XP055132437
NIYOM LUE; WONSHIK CHOI; GABRIEL POPESCU; KAMRAN BADIZADEGAN; RAMACHANDRA R. DASARI; MICHAEL S. FELD, OPTICS EXPRESS, vol. 16, no. 20, 2008, pages 16240 - 16246
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
Yoshiki Hasegawa (JP)
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Claims: