Title:
CELL CRYOPRESERVATION COMPOSITION AND CRYOPRESERVATION METHOD
Document Type and Number:
WIPO Patent Application WO/2018/143166
Kind Code:
A1
Abstract:
The present invention is added by a simple means when cells are to be cryopreserved and improves the survival rate of the cells. According to the present invention, cells are stored after 1 vol% of a composition containing 0.01-20 wt% of sophorose lipid is added thereto during a period between six hours before and immediately before cryopreservation of the cells. This composition is capable of reducing frost damage to cells, and thereby improves cell survival rate and enables storage of cells without using DMSO and blood serum. In addition, the present invention provides a simple and inexpensive storage method that does not require delicate control on freezing speed during cell cryopreservation.
Inventors:
NOGAMI ASUKA (JP)
TATSUMI MOTOKI (JP)
ISHII NANASE (JP)
RYU MIZUYUKI (JP)
HIRATA YOSHIHIKO (JP)
SAWA YOSHIKI (JP)
MIYAGAWA SHIGERU (JP)
SAITO ATSUHIRO (JP)
OHKAWARA HIROTATSU (JP)
TATSUMI MOTOKI (JP)
ISHII NANASE (JP)
RYU MIZUYUKI (JP)
HIRATA YOSHIHIKO (JP)
SAWA YOSHIKI (JP)
MIYAGAWA SHIGERU (JP)
SAITO ATSUHIRO (JP)
OHKAWARA HIROTATSU (JP)
Application Number:
PCT/JP2018/002879
Publication Date:
August 09, 2018
Filing Date:
January 30, 2018
Export Citation:
Assignee:
SARAYA CO LTD (JP)
UNIV OSAKA (JP)
UNIV OSAKA (JP)
International Classes:
C12N1/04; C12N5/071
Domestic Patent References:
| WO2011011055A2 | 2011-01-27 |
Foreign References:
| US5118512A | 1992-06-02 | |||
| JP2016160244A | 2016-09-05 | |||
| JP2010158192A | 2010-07-22 | |||
| JP2014117240A | 2014-06-30 | |||
| JPS5940975B2 | 1984-10-03 | |||
| JP2001247401A | 2001-09-11 | |||
| JP2010511402A | 2008-06-09 | |||
| JP2012235728A | 2012-12-06 | |||
| JPS5630979B2 | 1981-07-18 | |||
| JP2016160244A | 2016-09-05 |
Other References:
MAZUR, AM. J. PHYSIOL., vol. 247, 1984, pages C125 - 142
KOVELOCK JEBIOSHOP MWH, NATURE, vol. 183, 1959, pages 1394 - 1395
KOVELOCK JEBIOSHOP MWH, NATURE, vol. 183, 1959, pages 1394 - 1395
Attorney, Agent or Firm:
MAEDA & PARTNERS (JP)
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