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Title:
CELL CULTURE CONTAINER, METHOD FOR ACQUIRING CELLS, AND METHOD FOR CULTURING CELLS
Document Type and Number:
WIPO Patent Application WO/2019/069931
Kind Code:
A1
Abstract:
Provided is a cell culture container equipped with an inlet (21) for injecting a fluid, an outlet (22) for discharging a fluid, and a flow path (23) that connects the inlet and the outlet and accommodates a cell culture substrate (14) obtained by dispersing gold microparticles (13) in a gel denatured by heating. Also provided is a method for acquiring cells, the method including a step for selecting cells to be acquired from cells cultured in the cell culture container, a step for irradiating the gel with light in the vicinity of the selected cells, a step for allowing a liquid to flow into the flow path of the cell culture container, and a step for recovering cells from the outlet of the cell culture container.

Inventors:
KOJIMA CHIE (JP)
SHIMIZU TATSUYA (JP)
HARAGUCHI YUJI (JP)
KAWANO TAKESHI (JP)
TAKI YUSUKE (JP)
YOKOYAMA KAEDE (JP)
Application Number:
PCT/JP2018/036897
Publication Date:
April 11, 2019
Filing Date:
October 02, 2018
Export Citation:
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Assignee:
UNIV OSAKA PREFECT PUBLIC CORP (JP)
UNIV TOKYO WOMENS MEDICAL (JP)
NIKON CORP (JP)
International Classes:
C12M1/00; C12M3/00; C12N5/071
Domestic Patent References:
WO2015105029A12015-07-16
WO2018131661A12018-07-19
Foreign References:
JP2012039947A2012-03-01
JP2011244713A2011-12-08
JP2010011747A2010-01-21
JP2013233101A2013-11-21
JP2017000113A2017-01-05
JP2013013367A2013-01-24
JPH08172956A1996-07-09
JPH11349643A1999-12-21
JP2017193584A2017-10-26
JP2001349643A2001-12-21
JP2013233101A2013-11-21
Other References:
S. YAGI ET AL., JELECTROCHEM SOC, vol. 159, 2012, pages H668
BIOCHEM BIOPHYS RES COMMUN, vol. 425, 2012, pages 321
See also references of EP 3693452A4
Attorney, Agent or Firm:
NISHIZAWA Kazuyoshi et al. (JP)
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