CELL CULTURE DEVICE AND CELL CULTURE METHOD

WIPO Patent Application WO/2012/032646

A1

A cell culture device, comprising: an intermediate layer consisting of two porous membranes (14, 24); a first culture chamber (11a) and a second culture chamber (21a), said culture chambers being partitioned by the intermediate layer; and limiting layers (13, 23) which are positioned on both sides of the intermediate layer and provided with multiple openings (13a, 23a) for exposing the intermediate layer to a liquid flowing between the culture chambers (11a, 21a). Since the intermediate layer consists of the two porous membranes (14, 24), different scaffolds, which are suitable for the type of cells to be cultured, can be coated on both sides of the intermediate layer. Since areas to which cells can be adhered are limited by the limiting layers (13, 23), the cells can be fixed to the intermediate layer in a desired pattern that is suitable for the expression of the function thereof. Thus, an environment closer to the in vivo environment can be constructed in the device and cells can be cultured over a long period of time while sustaining the cell function.

FUJIYAMA YOICHI (JP)
TAGAWA YOH-ICHI (JP)

PCT/JP2010/065585

March 15, 2012

September 10, 2010

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SHIMADZU CORP (JP)
TOKYO INST TECH (JP)
FUJIYAMA YOICHI (JP)
TAGAWA YOH-ICHI (JP)

C12M1/00

WO2005100549A1

2005-10-27

JPH04278083A

1992-10-02

Kyoto International Patent Law Office (JP)
Patent business corporation Kyoto international patent firm (JP)

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