Title:
CELL CULTURE DEVICE AND CELL CULTURE METHOD
Document Type and Number:
WIPO Patent Application WO/2017/154899
Kind Code:
A1
Abstract:
This cell culture device is provided with a storage tank having one or more cell culture units. The cell culture units each have: a first liquid storage chamber that has an airtight structure, and in which a liquid is stored; a second liquid storage chamber in which the liquid is stored; a culture medium storage chamber that has a culture medium storage space in which a culture medium for cells is stored; a permeable diaphragm of which one surface, to which the cells can adhere, faces the culture medium storage space; and a liquid lead-out flow path that leads the liquid, which has the first liquid storage chamber as the supply source thereof, from the space at the side of the other surface of the diaphragm to the second liquid storage chamber. The storage tank has a vent hole that supplies and discharges a gas to and from the first liquid storage chamber.
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Inventors:
SUGIURA SHINJI (JP)
SATOH TAKU (JP)
KANAMORI TOSHIYUKI (JP)
SHIN KAZUMI (JP)
SATOH TAKU (JP)
KANAMORI TOSHIYUKI (JP)
SHIN KAZUMI (JP)
Application Number:
PCT/JP2017/008995
Publication Date:
September 14, 2017
Filing Date:
March 07, 2017
Export Citation:
Assignee:
NAT INST ADVANCED IND SCIENCE & TECH (JP)
International Classes:
C12M1/00; C12M1/04; C12M3/00; C12N5/07
Foreign References:
| JP2008086264A | 2008-04-17 | |||
| JP2011257238A | 2011-12-22 | |||
| JP2009527225A | 2009-07-30 | |||
| JP2009109249A | 2009-05-21 |
Other References:
IMURA Y ET AL.: "Micro Total Bioassay System for Ingested Substances: Assessment of Intestinal Absorption", HEPATIC METABOLISM, AND BIOACTIVITY , ANAL. CHEM., vol. 82, 2010, pages 9983 - 9988, XP055163485
HATTORI K ET AL.: "Microfluidic perfusion culture chip providing different strengths of shear stress for analysis of vascular endothelial function", J. BIOSCI. BIOENG., vol. 118, 2014, pages 327 - 332, XP055413921
SUGIURA S ET AL.: "Pressure-driven perfusion culture microchamber array for a parallel drug cytotoxicity assay", BIOTECHNOL. BIOENG., vol. 100, 2008, pages 1156 - 1165, XP055319428
PERESTRELO AR ET AL.: "Microfluidic Organ/Body- on-a-Chip Devices at the Convergence of Biology and Microengineering", SENSORS, vol. 15, 2015, pages 31142 - 31170, XP055413925
See also references of EP 3428266A4
HATTORI K ET AL.: "Microfluidic perfusion culture chip providing different strengths of shear stress for analysis of vascular endothelial function", J. BIOSCI. BIOENG., vol. 118, 2014, pages 327 - 332, XP055413921
SUGIURA S ET AL.: "Pressure-driven perfusion culture microchamber array for a parallel drug cytotoxicity assay", BIOTECHNOL. BIOENG., vol. 100, 2008, pages 1156 - 1165, XP055319428
PERESTRELO AR ET AL.: "Microfluidic Organ/Body- on-a-Chip Devices at the Convergence of Biology and Microengineering", SENSORS, vol. 15, 2015, pages 31142 - 31170, XP055413925
See also references of EP 3428266A4
Attorney, Agent or Firm:
SHIGA Masatake et al. (JP)
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