Title:
CELL CULTURE METHOD, CULTURE VESSEL, AND CELL CULTURE APPARATUS
Document Type and Number:
WIPO Patent Application WO/2018/105078
Kind Code:
A1
Abstract:
A cell culture method is one for culturing cells using a culture vessel equipped with a first vessel and a second vessel having a liquid pass and housed inside of the first vessel, wherein the liquid pass is so configured that a liquid can flow in or flow out therethrough. The method comprises the steps of: seeding cells in the second vessel; supplying a culture medium into the first vessel until the liquid level reaches a position higher than the liquid pass in the second vessel; and discharging the culture medium from the first vessel.
Inventors:
KIYAMA MASAHARU (JP)
HANZAWA HIROKO (JP)
MATSUMOTO ERINO (JP)
OHTA MARI (JP)
TAKEDA SHIZU (JP)
HANZAWA HIROKO (JP)
MATSUMOTO ERINO (JP)
OHTA MARI (JP)
TAKEDA SHIZU (JP)
Application Number:
PCT/JP2016/086516
Publication Date:
June 14, 2018
Filing Date:
December 08, 2016
Export Citation:
Assignee:
HITACHI LTD (JP)
International Classes:
C12N5/071; C12M3/00
Domestic Patent References:
| WO2016157322A1 | 2016-10-06 | |||
| WO2012008368A1 | 2012-01-19 | |||
| WO2015129577A1 | 2015-09-03 |
Foreign References:
| JP2007510429A | 2007-04-26 |
Other References:
"Permeable Supports Selection Guide", CORNING CLS-CC-027, April 2013 (2013-04-01), pages 2 - 6, XP055510515, Retrieved from the Internet [retrieved on 20170113]
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
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