Title:
CELL CULTURE METHOD
Document Type and Number:
WIPO Patent Application WO/2021/251312
Kind Code:
A1
Abstract:
The present invention provides a cell culture method that enables organ organoids to be cultured and matured over a long period of time, and is suitable for producing three-dimensional organs. The cell culture method of the present invention is for culturing, while perfusing a culture solution, organ organoids immobilized in a chamber and/or cells constituting an organ. The culture solution is perfused so as to create a turbulent flow in the chamber.
Inventors:
TAKEBE TAKANORI (JP)
SAIKI NORIKAZU (JP)
SAIKI NORIKAZU (JP)
Application Number:
PCT/JP2021/021478
Publication Date:
December 16, 2021
Filing Date:
June 07, 2021
Export Citation:
Assignee:
UNIV NAT CORP TOKYO MEDICAL & DENTAL (JP)
TAKEDA PHARMACEUTICALS CO (JP)
TAKEDA PHARMACEUTICALS CO (JP)
International Classes:
C12N5/071; C12M3/00
Domestic Patent References:
| WO2013047639A1 | 2013-04-04 | |||
| WO2015129822A1 | 2015-09-03 | |||
| WO2013047639A1 | 2013-04-04 | |||
| WO2015129822A1 | 2015-09-03 | |||
| WO2013047720A1 | 2013-04-04 |
Foreign References:
| JP2020010683A | 2020-01-23 | |||
| JPH0276569A | 1990-03-15 | |||
| JP2015510391A | 2015-04-09 | |||
| US20140030762A1 | 2014-01-30 | |||
| JP2018110575A | 2018-07-19 |
Other References:
TAKEBE, TAKANORI ET AL.: "Regenerative medicine and gene therapy have begun to bear fruit: Current status of regenerative medicine for liver diseases", JAPANESE JOURNAL OF PEDIATRIC MEDICINE, vol. 49, no. 7, 30 November 2016 (2016-11-30), pages 981 - 986, XP009541744, ISSN: 0385-6305
WEYAND BIRGIT, ISRAELOWITZ MEIR, KRAMER JAMES, BODMER CHRISTIAN, NOEHRE MARIEL, STRAUSS SARAH, SCHMÄLZLIN ELMAR, GILLE CHRISTOPH, : "Three-Dimensional Modelling inside a Differential Pressure Laminar Flow Bioreactor Filled with Porous Media", BIOMED RESEARCH INTERNATIONAL, HINDAWI PUBLISHING CORPORATION, vol. 2015, 1 January 2015 (2015-01-01), pages 1 - 9, XP055888758, ISSN: 2314-6133, DOI: 10.1155/2015/320280
HOMAN, K. A. ET AL.: "Flow-enhanced vascularization and maturation of kidney organoids in vitro", NAT METHODS, vol. 16, 2019, pages 255 - 262, XP036713088, DOI: 10.1038/s41592-019-0325-y
OTA, N. ET AL.: "A Microfluidic Platform Based on Robust Gas and Liquid Exchange for Long term Culturing of Explanted Tissues", ANALYTICAL SCIENCES, vol. 35, October 2019 (2019-10-01)
"Long term Culturing of Explanted Tissues", ANALYTICAL SCIENCES, vol. 35, October 2019 (2019-10-01)
K. SI-TAIYEB ET AL., HEPATOLOGY, vol. 51, no. 1, 2010, pages 1754 - 305
CELL REP, vol. 21, no. 10, 2017, pages 2661 - 2670
PLOS ONE, vol. 8, no. 4, 2013, pages e59243
NAT BIOTECHNOL., vol. 32, no. 6, 2014, pages 554 - 61
SCI REP, vol. 6, 2016, pages 35680
MORIZANE ET AL., NAT PROTOC, vol. 12, 2017, pages 195 - 207
TAGUCHI ET AL., CELL STEM CELL, vol. 14, no. 1, 2014, pages 53 - 67
NARAYANAN ET AL., KIDNEY INT, vol. 83, no. 4, 2013, pages 593 - 603
WEYAND BIRGIT, ISRAELOWITZ MEIR, KRAMER JAMES, BODMER CHRISTIAN, NOEHRE MARIEL, STRAUSS SARAH, SCHMÄLZLIN ELMAR, GILLE CHRISTOPH, : "Three-Dimensional Modelling inside a Differential Pressure Laminar Flow Bioreactor Filled with Porous Media", BIOMED RESEARCH INTERNATIONAL, HINDAWI PUBLISHING CORPORATION, vol. 2015, 1 January 2015 (2015-01-01), pages 1 - 9, XP055888758, ISSN: 2314-6133, DOI: 10.1155/2015/320280
HOMAN, K. A. ET AL.: "Flow-enhanced vascularization and maturation of kidney organoids in vitro", NAT METHODS, vol. 16, 2019, pages 255 - 262, XP036713088, DOI: 10.1038/s41592-019-0325-y
OTA, N. ET AL.: "A Microfluidic Platform Based on Robust Gas and Liquid Exchange for Long term Culturing of Explanted Tissues", ANALYTICAL SCIENCES, vol. 35, October 2019 (2019-10-01)
"Long term Culturing of Explanted Tissues", ANALYTICAL SCIENCES, vol. 35, October 2019 (2019-10-01)
K. SI-TAIYEB ET AL., HEPATOLOGY, vol. 51, no. 1, 2010, pages 1754 - 305
CELL REP, vol. 21, no. 10, 2017, pages 2661 - 2670
PLOS ONE, vol. 8, no. 4, 2013, pages e59243
NAT BIOTECHNOL., vol. 32, no. 6, 2014, pages 554 - 61
SCI REP, vol. 6, 2016, pages 35680
MORIZANE ET AL., NAT PROTOC, vol. 12, 2017, pages 195 - 207
TAGUCHI ET AL., CELL STEM CELL, vol. 14, no. 1, 2014, pages 53 - 67
NARAYANAN ET AL., KIDNEY INT, vol. 83, no. 4, 2013, pages 593 - 603
Attorney, Agent or Firm:
OHNO Seiji et al. (JP)
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