Title:
CELL CULTURE METHOD
Document Type and Number:
WIPO Patent Application WO/2023/282273
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method for enabling, in mass production of human induced pluripotent stem cell (hiPS cell)-derived cardiomyocyte aggregates, the production of homogeneous and high-quality cardiomyocyte aggregates without using a living body-derived serum. The present invention provides a method for producing human induced pluripotent stem cell (hiPS cell)-derived cardiomyocyte aggregates, comprising: a step for forming, on a substrate having the ability to inhibit the adhesion of cells, a base membrane for cardiomyocyte culture that includes a cell adhesive substance and a polymer which contains a repeating unit derived from a monomer represented by formula (I); and a step for then seeding cardiomyocytes.
(I)
[In formula (1), Ua1, Ua2, Ra1, and Ra2 are as described in the specification and claims.]
Inventors:
TOHYAMA SHUGO (JP)
FUKUDA KEIICHI (JP)
SUZUKI KOHEI (JP)
UEDA YUKI (JP)
HIROI MIYA (JP)
HIROI YOSHIOMI (JP)
KANAAMI SAYAKA (JP)
FUKUDA KEIICHI (JP)
SUZUKI KOHEI (JP)
UEDA YUKI (JP)
HIROI MIYA (JP)
HIROI YOSHIOMI (JP)
KANAAMI SAYAKA (JP)
Application Number:
PCT/JP2022/026773
Publication Date:
January 12, 2023
Filing Date:
July 05, 2022
Export Citation:
Assignee:
UNIV KEIO (JP)
NISSAN CHEMICAL CORP (JP)
HEARTSEED INC (JP)
NISSAN CHEMICAL CORP (JP)
HEARTSEED INC (JP)
International Classes:
C12N5/077; C08F20/34; C12N5/10
Domestic Patent References:
| WO2020040247A1 | 2020-02-27 | |||
| WO2014196650A1 | 2014-12-11 |
Foreign References:
| JPH05292957A | 1993-11-09 | |||
| JP2014162865A | 2014-09-08 | |||
| JP2021091872A | 2021-06-17 | |||
| JP2017055744A | 2017-03-23 |
Attorney, Agent or Firm:
TSUKUNI & ASSOCIATES et al. (JP)
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