Title:
CELL DEATH INDUCER
Document Type and Number:
WIPO Patent Application WO/2008/007755
Kind Code:
A1
Abstract:
The object is to provide an antibody having a high cell death-inducing activity. To achieve the object, a mouse is immunized with a cell capable of expressing both human HLA class IA and human β2 microglobulin (β2M) to produce monoclonal antibodies. The monoclonal antibodies are screened for an antibody having a cell death-inducing activity, and 10 clones are obtained. The analysis of the clones reveals that three clones (C3B3, C11B9, C17D11 antibodies) which has an α2 domain of HLA class I antigen in their epitopes can cross-link with an anti-murine IgG antibody to show a potent cytotoxic activity. When a diabody of the C3B3 antibody is produced, it is found that the diabody shows a more potent anti-tumor effect compared to that of a diabody of a 2D7 antibody which is a known antibody against HLA class IA.
Inventors:
KIMURA NAOKI (JP)
KAWAI SHIGETO (JP)
KAWAI SHIGETO (JP)
Application Number:
PCT/JP2007/063946
Publication Date:
January 17, 2008
Filing Date:
July 13, 2007
Export Citation:
Assignee:
CHUGAI PHARMACEUTICAL CO LTD (JP)
KIMURA NAOKI (JP)
KAWAI SHIGETO (JP)
KIMURA NAOKI (JP)
KAWAI SHIGETO (JP)
International Classes:
C12N15/00; A61K39/395; A61P7/00; A61P35/00; C07K16/18; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; C12P21/08
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Other References:
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GENESTIER L. ET AL.: "Fas-independent apoptosis of activated T cells induced by antibodies to the HLA class I alpha1 domain", BLOOD, vol. 90, no. 9, 1997, pages 3629 - 3639, XP002964772
FAYEN ET AL., INT. IMMUNOL., vol. 10, 1998, pages 1347 - 1358
GENESTIER ET AL., BLOOD, vol. 90, 1997, pages 3629 - 3639
GENESTIER ET AL., BLOOD, vol. 90, 1997, pages 726 - 735
GENESTIER ET AL., J. BIOL. CHEM., vol. 273, 1998, pages 5060 - 5066
WOODLE ET AL., J. IMMUNOL., vol. 158, 1997, pages 2156 - 2164
MATSUOKA, J. EXP. MED., vol. 181, 1995, pages 2007 - 2015
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KIMURA ET AL., BIOCHEM BIOPHYS RES COMMUN., vol. 325, 2004, pages 1201 - 1209
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ZOLLER, MJ; SMITH, M., METHODS ENZYMOL., vol. 100, 1983, pages 468 - 500
KRAMER, W. ET AL., NUCLEIC ACIDS RES., vol. 12, 1984, pages 9441 - 9456
KRAMER W; FRITZ HJ, METHODS. ENZYMOL., vol. 154, 1987, pages 350 - 367
KUNKEL, TA, PROC NATL. ACAD. SCI. USA., vol. 82, 1985, pages 488 - 492
KUNKEL, METHODS ENZYMOL., vol. 85, 1988, pages 2763 - 2766
MARK, D. F. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 81, 1984, pages 5662 - 5666
ZOLLER, M. J.; SMITH, M., NUCLEIC ACIDS RESEARCH, vol. 10, 1982, pages 6487 - 6500
WANG, A. ET AL., SCIENCE, vol. 224, pages 1431 - 1433
DALBADIE-MCFARLAND, G. ET AL., PROC. NATL. ACAD. SCI., vol. 79, 1982, pages 6409 - 6413
HOPP, T.P. ET AL., BIOTECHNOLOGY, vol. 6, 1988, pages 1204 - 1210
CARL, A. K. BORREBAECK; JAMES, W. LARRICK: "THERAPEUTIC MONOCLONAL ANTIBODIES", 1990, MACMILLAN PUBLISHERS LTD
SAMBROOK, J. ET AL.: "Molecular Cloning", 1989, COLD SPRING HARBOR LAB. PRESS, pages: 9.47 - 9.58
WILBUR, W. J.; LIPMAN, D. J., PROC. NATL. ACAD. SCI. USA, vol. 80, 1983, pages 726 - 730
WARD ET AL., NATURE, vol. 341, 1989, pages 544 - 546
FASEB J., vol. 6, 1992, pages 2422 - 2427
BETTER ET AL., SCIENCE, vol. 240, 1988, pages 1041 - 1043
LEI, S. P. ET AL., J. BACTERIOL., vol. 169, 1987, pages 4379
NUCLEIC ACIDS RES., vol. 18, no. 17, 1990, pages 5322
MULLIGAN ET AL., NATURE, vol. 277, 1979, pages 108
MIZUSHIMA ET AL., NUCLEIC ACIDS RES., vol. 18, 1990, pages 5322
J. EXP. MED., vol. 108, 1995, pages 945
VALLE ET AL., NATURE, vol. 291, 1981, pages 358 - 340
PROC. NATL. ACAD. SCI. USA, vol. 77, 1980, pages 4216 - 4220
PROC. NATL. ACAD. SCI. USA, vol. 60, 1968, pages 1275
VICKI GLASER, SPECTRUM BIOTECHNOLOGY APPLICATIONS, 1993
EBERT, K.M. ET AL., BIO/TECHNOLOGY, vol. 12, 1994, pages 699 - 702
SUSUMU, M. ET AL., NATURE, vol. 315, 1985, pages 592 - 594
JULIAN K.-C. MA ET AL., EUR. J. IMMUNOL., vol. 24, 1994, pages 131 - 138
"Strategies for Protein Purification and Characterization: A Laboratory Course Manual", 1996, COLD SPRING HARBOR LABORATORY PRESS
"Antibodies A Laboratory Manual", 1988, COLD SPRING HARBOR LABORATORY
SATO, K. ET AL., CANCER RES., vol. 53, 1993, pages 851 - 856
HUSTON, J. S. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 85, 1988, pages 5879 - 5883
PLICKTHUN: "The Pharmacology of Monoclonal Antibodies", vol. 113, 1994, SPRINGER VERLAG, pages: 269 - 315
CO, M. S. ET AL., J. IMMUNOL., vol. 152, 1994, pages 2968 - 2976
BETTER, M.; HORWITZ, A. H., METHODS ENZYMOL., vol. 178, 1989, pages 476 - 496
PLUCKTHUN, A.; SKERRA, A., METHODS ENZYMOL., vol. 178, 1989, pages 497 - 515
LAMOYI, E., METHODS ENZYMOL., vol. 121, 1986, pages 652 - 663
ROUSSEAUX, J. ET AL., METHODS ENZYMOL., vol. 121, 1986, pages 663 - 669
BIRD, R. E.; WALKER, B. W., TRENDS BIOTECHNOL., vol. 9, 1991, pages 132 - 137
P. HOLLIGER ET AL., PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 6444 - 6448
JOHNSON ET AL., METHOD IN ENZYMOLOGY, vol. 203, 1991, pages 88 - 98
HOLLIGER ET AL., PROTEIN ENGINEERING, vol. 9, 1996, pages 299 - 305
PERISIC ET AL., STRUCTURE, vol. 2, 1994, pages 1217 - 1226
JOHN ET AL., PROTEIN ENGINEERING, vol. 12, no. 7, 1999, pages 597 - 604
HOLLIGER ET AL., PROC. NATL. ACAD. SCI. USA., vol. 90, 1993, pages 6444 - 6448
ATWELL, MOL. IMMUNOL., vol. 33, 1996, pages 1301 - 1312
KOHLER, G; MILSTEIN, C., METHODS ENZYMOL., vol. 73, 1981, pages 3 - 46
"Remington's Pharmaceutical Science", 1980
LANGER ET AL., J. BIOMED. MATER. RES., vol. 15, 1981, pages 167 - 277
LANGER, CHEM. TECH., vol. 12, 1982, pages 98 - 105
SIDMAN ET AL., BIOPOLYMERS, vol. 22, 1983, pages 547 - 556
See also references of EP 2048230A4
GENESTIER L. ET AL.: "Fas-independent apoptosis of activated T cells induced by antibodies to the HLA class I alpha1 domain", BLOOD, vol. 90, no. 9, 1997, pages 3629 - 3639, XP002964772
FAYEN ET AL., INT. IMMUNOL., vol. 10, 1998, pages 1347 - 1358
GENESTIER ET AL., BLOOD, vol. 90, 1997, pages 3629 - 3639
GENESTIER ET AL., BLOOD, vol. 90, 1997, pages 726 - 735
GENESTIER ET AL., J. BIOL. CHEM., vol. 273, 1998, pages 5060 - 5066
WOODLE ET AL., J. IMMUNOL., vol. 158, 1997, pages 2156 - 2164
MATSUOKA, J. EXP. MED., vol. 181, 1995, pages 2007 - 2015
GOTO ET AL., BLOOD, vol. 84, 1994, pages 1922 - 30
KIMURA ET AL., BIOCHEM BIOPHYS RES COMMUN., vol. 325, 2004, pages 1201 - 1209
OKA, T., SANKYO SEIMEI-KAGAKU-ZAIDAN KENKYU HOKOKU-SHU, vol. 12, 1998, pages 46 - 56
HASHIMOTO-GOTOH, T. ET AL., GENE, vol. 152, 1995, pages 271 - 275
ZOLLER, MJ; SMITH, M., METHODS ENZYMOL., vol. 100, 1983, pages 468 - 500
KRAMER, W. ET AL., NUCLEIC ACIDS RES., vol. 12, 1984, pages 9441 - 9456
KRAMER W; FRITZ HJ, METHODS. ENZYMOL., vol. 154, 1987, pages 350 - 367
KUNKEL, TA, PROC NATL. ACAD. SCI. USA., vol. 82, 1985, pages 488 - 492
KUNKEL, METHODS ENZYMOL., vol. 85, 1988, pages 2763 - 2766
MARK, D. F. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 81, 1984, pages 5662 - 5666
ZOLLER, M. J.; SMITH, M., NUCLEIC ACIDS RESEARCH, vol. 10, 1982, pages 6487 - 6500
WANG, A. ET AL., SCIENCE, vol. 224, pages 1431 - 1433
DALBADIE-MCFARLAND, G. ET AL., PROC. NATL. ACAD. SCI., vol. 79, 1982, pages 6409 - 6413
HOPP, T.P. ET AL., BIOTECHNOLOGY, vol. 6, 1988, pages 1204 - 1210
CARL, A. K. BORREBAECK; JAMES, W. LARRICK: "THERAPEUTIC MONOCLONAL ANTIBODIES", 1990, MACMILLAN PUBLISHERS LTD
SAMBROOK, J. ET AL.: "Molecular Cloning", 1989, COLD SPRING HARBOR LAB. PRESS, pages: 9.47 - 9.58
WILBUR, W. J.; LIPMAN, D. J., PROC. NATL. ACAD. SCI. USA, vol. 80, 1983, pages 726 - 730
WARD ET AL., NATURE, vol. 341, 1989, pages 544 - 546
FASEB J., vol. 6, 1992, pages 2422 - 2427
BETTER ET AL., SCIENCE, vol. 240, 1988, pages 1041 - 1043
LEI, S. P. ET AL., J. BACTERIOL., vol. 169, 1987, pages 4379
NUCLEIC ACIDS RES., vol. 18, no. 17, 1990, pages 5322
MULLIGAN ET AL., NATURE, vol. 277, 1979, pages 108
MIZUSHIMA ET AL., NUCLEIC ACIDS RES., vol. 18, 1990, pages 5322
J. EXP. MED., vol. 108, 1995, pages 945
VALLE ET AL., NATURE, vol. 291, 1981, pages 358 - 340
PROC. NATL. ACAD. SCI. USA, vol. 77, 1980, pages 4216 - 4220
PROC. NATL. ACAD. SCI. USA, vol. 60, 1968, pages 1275
VICKI GLASER, SPECTRUM BIOTECHNOLOGY APPLICATIONS, 1993
EBERT, K.M. ET AL., BIO/TECHNOLOGY, vol. 12, 1994, pages 699 - 702
SUSUMU, M. ET AL., NATURE, vol. 315, 1985, pages 592 - 594
JULIAN K.-C. MA ET AL., EUR. J. IMMUNOL., vol. 24, 1994, pages 131 - 138
"Strategies for Protein Purification and Characterization: A Laboratory Course Manual", 1996, COLD SPRING HARBOR LABORATORY PRESS
"Antibodies A Laboratory Manual", 1988, COLD SPRING HARBOR LABORATORY
SATO, K. ET AL., CANCER RES., vol. 53, 1993, pages 851 - 856
HUSTON, J. S. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 85, 1988, pages 5879 - 5883
PLICKTHUN: "The Pharmacology of Monoclonal Antibodies", vol. 113, 1994, SPRINGER VERLAG, pages: 269 - 315
CO, M. S. ET AL., J. IMMUNOL., vol. 152, 1994, pages 2968 - 2976
BETTER, M.; HORWITZ, A. H., METHODS ENZYMOL., vol. 178, 1989, pages 476 - 496
PLUCKTHUN, A.; SKERRA, A., METHODS ENZYMOL., vol. 178, 1989, pages 497 - 515
LAMOYI, E., METHODS ENZYMOL., vol. 121, 1986, pages 652 - 663
ROUSSEAUX, J. ET AL., METHODS ENZYMOL., vol. 121, 1986, pages 663 - 669
BIRD, R. E.; WALKER, B. W., TRENDS BIOTECHNOL., vol. 9, 1991, pages 132 - 137
P. HOLLIGER ET AL., PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 6444 - 6448
JOHNSON ET AL., METHOD IN ENZYMOLOGY, vol. 203, 1991, pages 88 - 98
HOLLIGER ET AL., PROTEIN ENGINEERING, vol. 9, 1996, pages 299 - 305
PERISIC ET AL., STRUCTURE, vol. 2, 1994, pages 1217 - 1226
JOHN ET AL., PROTEIN ENGINEERING, vol. 12, no. 7, 1999, pages 597 - 604
HOLLIGER ET AL., PROC. NATL. ACAD. SCI. USA., vol. 90, 1993, pages 6444 - 6448
ATWELL, MOL. IMMUNOL., vol. 33, 1996, pages 1301 - 1312
KOHLER, G; MILSTEIN, C., METHODS ENZYMOL., vol. 73, 1981, pages 3 - 46
"Remington's Pharmaceutical Science", 1980
LANGER ET AL., J. BIOMED. MATER. RES., vol. 15, 1981, pages 167 - 277
LANGER, CHEM. TECH., vol. 12, 1982, pages 98 - 105
SIDMAN ET AL., BIOPOLYMERS, vol. 22, 1983, pages 547 - 556
See also references of EP 2048230A4
Attorney, Agent or Firm:
SHIMIZU, Hatsushi et al. (1-1-1 Oroshi-machi,Tsuchiura-sh, Ibaraki 47, JP)
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