Title:
CELL-FREE DNA MONITORING
Document Type and Number:
WIPO Patent Application WO/2021/142437
Kind Code:
A1
Abstract:
Methods and compositions for monitoring mutation burden, cancer status, vaccine efficacy using cell-free DNA sequencing are disclosed. In some aspects, the method comprises the steps of: a. obtaining or having obtained sequencing data of cell-free DNA (cfDNA) from a sample from the subject, and wherein the sequencing data comprises a target coverage of at least 50% of all polynucleotide regions of interest corresponding to mutations present in an exome of the cancer and wherein the sequenced polynucleotide regions of interest have a mean read depth of at least 1000X.
Inventors:
SUN JAMES (US)
DAVIS MATTHEW (US)
SCHENK DESIREE (US)
NAVARRO GOMEZ DANIEL (US)
DAVIS MATTHEW (US)
SCHENK DESIREE (US)
NAVARRO GOMEZ DANIEL (US)
Application Number:
PCT/US2021/012945
Publication Date:
July 15, 2021
Filing Date:
January 11, 2021
Export Citation:
Assignee:
GRITSTONE BIO INC (US)
International Classes:
C12Q1/68; C12Q1/6869; C12Q1/6886; G16B20/00; G16B20/20; G16B30/00
Domestic Patent References:
| WO2019170773A1 | 2019-09-12 | |||
| WO2019090156A1 | 2019-05-09 |
Foreign References:
| US20180363066A1 | 2018-12-20 | |||
| US20190025308A1 | 2019-01-24 | |||
| US20190256924A1 | 2019-08-22 |
Other References:
WAN JONATHAN C. M., HEIDER KATRIN, GALE DAVINA, MURPHY SUZANNE, FISHER EYAL, MORRIS JAMES, MOULIERE FLORENT, CHANDRANANDA DINEIKA,: "High-sensitivity monitoring of ctDNA by patient-specific sequencing panels and integration of variant reads", BIORXIV, 11 September 2019 (2019-09-11), pages 1 - 37, XP055840219, DOI: 10.1101/759399
See also references of EP 4087940A4
See also references of EP 4087940A4
Attorney, Agent or Firm:
NEWMAN, Zachary, R. et al. (US)
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