Title:
CELL-FREE AND MINIMIZED METABOLIC REACTION CASCADES FOR THE PRODUCTION OF CHEMICALS
Document Type and Number:
WIPO Patent Application WO/2014/029761
Kind Code:
A3
Abstract:
Provided are enzymatic processes for the production of chemicals like ethanol from carbon sources like glucose, in particular, a process for the production of a target chemical is disclosed using a cell- free enzyme system that converts carbohydrate sources to the intermediate pyruvate and subsequently the intermediate pyruvate to the target chemical wherein a minimized number of enzymes and only one cofactor is employed.
Inventors:
KRAUS MICHAEL (DE)
KOLTERMANN ANDRÉ (DE)
KETTLING ULRICH (DE)
GARBE DANIEL (DE)
BRÜCK THOMAS (DE)
GUTERL JAN-KARL (DE)
SIEBER VOLKER (DE)
KOLTERMANN ANDRÉ (DE)
KETTLING ULRICH (DE)
GARBE DANIEL (DE)
BRÜCK THOMAS (DE)
GUTERL JAN-KARL (DE)
SIEBER VOLKER (DE)
Application Number:
PCT/EP2013/067291
Publication Date:
April 17, 2014
Filing Date:
August 20, 2013
Export Citation:
Assignee:
CLARIANT PRODUKTE DEUTSCHLAND (DE)
TU MÜNCHEN (DE)
TU MÜNCHEN (DE)
International Classes:
C12P7/06; C12N9/00; C12N15/10; C12P7/16
Foreign References:
| EP2204453A1 | 2010-07-07 |
Other References:
REHER M ET AL: "Glyceraldehyde dehydrogenases from the thermoacidophilic euryarchaeota Picrophilus torridus and Thermoplasma acidophilum, key enzymes of the non-phosphorylative Entner-Doudoroff pathway, constitute a novel enzyme family within the aldehyde dehydrogenase superfamily", FEBS LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 580, no. 5, 20 February 2006 (2006-02-20), pages 1198 - 1204, XP028030201, ISSN: 0014-5793, [retrieved on 20060220], DOI: 10.1016/J.FEBSLET.2006.01.029
JAN-KARL GUTERL ET AL: "Cell-Free Metabolic Engineering: Production of Chemicals by Minimized Reaction Cascades", CHEMSUSCHEM, vol. 5, no. 11, 19 October 2012 (2012-10-19), pages 2165 - 2172, XP055086976, ISSN: 1864-5631, DOI: 10.1002/cssc.201200365
JAN-KARL GUTERL ET AL: "Biosynthesis "debugged": Novel bioproduction strategies", ENGINEERING IN LIFE SCIENCES, vol. 13, no. 1, 1 October 2012 (2012-10-01), pages 4 - 18, XP055087230, ISSN: 1618-0240, DOI: 10.1002/elsc.201100231
FABIAN STEFFLER ET AL: "Refolding of a Thermostable Glyceraldehyde Dehydrogenase for Application in Synthetic Cascade Biomanufacturing", PLOS ONE, vol. 8, no. 7, 24 July 2013 (2013-07-24), pages e70592, XP055086844, DOI: 10.1371/journal.pone.0070592
JAN-KARL GUTERL ET AL: "Cell-Free Metabolic Engineering: Production of Chemicals by Minimized Reaction Cascades", CHEMSUSCHEM, vol. 5, no. 11, 19 October 2012 (2012-10-19), pages 2165 - 2172, XP055086976, ISSN: 1864-5631, DOI: 10.1002/cssc.201200365
JAN-KARL GUTERL ET AL: "Biosynthesis "debugged": Novel bioproduction strategies", ENGINEERING IN LIFE SCIENCES, vol. 13, no. 1, 1 October 2012 (2012-10-01), pages 4 - 18, XP055087230, ISSN: 1618-0240, DOI: 10.1002/elsc.201100231
FABIAN STEFFLER ET AL: "Refolding of a Thermostable Glyceraldehyde Dehydrogenase for Application in Synthetic Cascade Biomanufacturing", PLOS ONE, vol. 8, no. 7, 24 July 2013 (2013-07-24), pages e70592, XP055086844, DOI: 10.1371/journal.pone.0070592
Attorney, Agent or Firm:
RENKEN, Joachim (HOFFMANN · EITLE) et al. (Munich, DE)
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