Title:
CELL ISOLATION DEVICE AND CELL ISOLATION METHOD
Document Type and Number:
WIPO Patent Application WO/2017/068972
Kind Code:
A1
Abstract:
The present invention provides a cell isolation device and cell isolation method capable of selecting cells having high antibody-production ability, from a candidate cell group by using a simple process but causing less damage to the cells. The cell isolation device (100) is provided with an operation part (101) in which a culture vessel having a plurality of wells is placed, an inoculation device (103) for inoculating candidate cells into a culture medium, a temperature adjustment device (102) for adjusting the temperature of the culture medium, a reagent addition device (106) for adding an anti-membrane protein antibody to the culture medium, and a collection device (104) for collecting the culture medium. A protein-binding site having an antibody-binding ability, and a temperature responsive site of which the three-dimensional structure changes depending on a change in temperature, are bound to the inner wall surface of the wells. The anti-membrane protein antibody is added to the cultured candidate cells, and the temperature of the culture medium is changed to a temperature exceeding the phase transition temperature of the temperature responsive site, whereby only antibody-producing cells are isolated and collected.
Inventors:
SHIBUYA KEISUKE (JP)
MARUYAMA MASASHI (JP)
HIRAI CHIDURU (JP)
MARUYAMA MASASHI (JP)
HIRAI CHIDURU (JP)
Application Number:
PCT/JP2016/079547
Publication Date:
April 27, 2017
Filing Date:
October 04, 2016
Export Citation:
Assignee:
HITACHI LTD (JP)
International Classes:
C12M1/00; B01D15/00; B01J20/26; B01J20/34; C12M3/00; C12N1/02; C12N5/0781
Domestic Patent References:
| WO2015033457A1 | 2015-03-12 | |||
| WO2016143215A1 | 2016-09-15 |
Foreign References:
| JP2006223195A | 2006-08-31 | |||
| JP2016163550A | 2016-09-08 |
Attorney, Agent or Firm:
ISONO INTERNATIONAL PATENT OFFICE, P.C. (JP)
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