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Title:
CELL ISOLATION METHOD AND CELL TRAPPING FILTER
Document Type and Number:
WIPO Patent Application WO/2016/068249
Kind Code:
A1
Abstract:
A cell isolation method comprises: a cell trapping step of allowing a solution of interest to pass through a cell trapping filter having multiple through-holes formed therein as observed in the thickness direction, thereby trapping isolation target cells contained in the solution of interest on one surface of the cell trapping filter; a gel embedment step of introducing a stimulus-responsive hydrogel onto the one surface of the cell trapping filter on which the cells have been trapped in the cell trapping step, thereby embedding the cells in the stimulus-responsive hydrogel; a gel hardening step of applying a stimulus to the stimulus-responsive hydrogel having the cells embedded therein, thereby hardening the stimulus-responsive hydrogel; and a detachment step of detaching the stimulus-responsive hydrogel that has been hardened in the gel hardening step from the cell trapping filter.

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Inventors:
YOSHINO TOMOKO (JP)
TANAKA TSUYOSHI (JP)
MATSUNAGA TADASHI (JP)
NEGISHI RYO (JP)
KANBARA HISASHIGE (JP)
NAKAMURA SEITA (JP)
Application Number:
PCT/JP2015/080588
Publication Date:
May 06, 2016
Filing Date:
October 29, 2015
Export Citation:
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Assignee:
UNIV TOKYO NAT UNIV CORP (JP)
HITACHI CHEMICAL CO LTD (JP)
International Classes:
C12N1/02; C12M1/00; C12M1/12; C12M3/06
Domestic Patent References:
WO2013172265A12013-11-21
WO2015012315A12015-01-29
Foreign References:
JP2014508516A2014-04-10
US20120208255A12012-08-16
JP2012092137A2012-05-17
JP2010518812A2010-06-03
JP2008507293A2008-03-13
JP2008043782A2008-02-28
JP2005261339A2005-09-29
JP2005102628A2005-04-21
JPS626690A1987-01-13
JP2015188323A2015-11-02
Other References:
See also references of EP 3214166A4
Attorney, Agent or Firm:
HASEGAWA YOSHIKI (JP)
Yoshiki Hasegawa (JP)
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