Title:
CELL LINE CAPABLE OF IMPLEMENTING INDUCED RESCUE OF RHABDOVIRUS, CONSTRUCTION METHOD THEREFOR, AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2019/061691
Kind Code:
A1
Abstract:
A cell line capable of implementing inducible rescue of rhabdovirus, construction method therefor, and application thereof. The cell line is a BHK(TetOn) cell line for lentivirus inducible expression of T7pol/P/N. The rhabdovirus construction method comprises: transfecting dual plasmids, namely a virus polymerase gene plasmid pL and a rhabdovirus core framework plasmid pCore, into the BHK(T7pol/P/N-TetOn) cell line according to a specific ratio, thereby rapidly obtaining a recombinant rhabdovirus. A vector for inducible expression of T7pol/P/N is integrated into a BHK(TetOn) cell genome by means of pGag/Pol, pRSV-Rev, and a pMD2.G lentivirus plasmid system. The cell line can efficiently perform rapid rescue on rhabdovirus assembly and completes screening and identification, and greatly improves the rescue efficiency of a recombinant rhabdovirus as compared with the conventional multi-plasmid rescue system.
Inventors:
WANG HONGWEI (CN)
XU ZHANGMIN (CN)
XU ZHANGMIN (CN)
Application Number:
PCT/CN2017/109964
Publication Date:
April 04, 2019
Filing Date:
November 08, 2017
Export Citation:
Assignee:
WANG HONGWEI (CN)
International Classes:
C12N5/10; C12N7/00; C12N15/85; C12N15/867; C12N15/90; C12R1/93
Foreign References:
| CN1871355A | 2006-11-29 | |||
| CN105505880A | 2016-04-20 | |||
| CN1769433A | 2006-05-10 | |||
| CN1441842A | 2003-09-10 | |||
| CN101631798A | 2010-01-20 | |||
| CN101087886A | 2007-12-12 | |||
| CN102026645A | 2011-04-20 |
Attorney, Agent or Firm:
NANJING JINGWEI PATENT & TRADEMARK AGENCY CO., LTD. (CN)
Download PDF: