Title:
CELL OBSERVATION APPARATUS, METHOD OF CELL OBSERVATION AND CELL OBSERVATION PROGRAM
Document Type and Number:
WIPO Patent Application WO/2006/120924
Kind Code:
A1
Abstract:
It is intended to, while minimizing any damage on cells during long-term culture, enable judging of whether cells during culture are dead or alive with high precision without the need of introducing of special gene or special dye. Noting that cells having undergone cell death without suffering physical impairment during culture become approximately circular in configuration, directly stop movements and maintain the approximately circular configuration, whether cells are dead or alive is judged (steps S736 and S737) by comparing with a threshold value the degree of circularity as a cell parameter indicating the characteristic of cell region (Rt,m) having been photographed and measured at multiple points of time (steps S732 to S735). As a result, while minimizing any damage on cells during long-term culture, whether cells during culture are dead or alive can be judged with high precision without the need of introducing of special gene or special dye.
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Inventors:
ARAI SATOSHI (JP)
Application Number:
PCT/JP2006/308888
Publication Date:
November 16, 2006
Filing Date:
April 27, 2006
Export Citation:
Assignee:
OLYMPUS CORP (JP)
ARAI SATOSHI (JP)
ARAI SATOSHI (JP)
International Classes:
C12M1/34; C12Q1/04; G01N21/27; G01N21/64; G01N33/48; G01N33/483
Domestic Patent References:
| WO2002052032A1 | 2002-07-04 |
Foreign References:
| JP2005027623A | 2005-02-03 | |||
| JPH05184579A | 1993-07-27 | |||
| JP2004175692A | 2004-06-24 | |||
| JP2005532031A | 2005-10-27 | |||
| JP2002510291A | 2002-04-02 | |||
| JPH05184579A | 1993-07-27 | |||
| JP2000316596A | 2000-11-21 | |||
| JPH0377628B2 | 1991-12-11 | |||
| JP2004113175A | 2004-04-15 |
Other References:
TOMASI; MANDUCHI: "Bilateral Filtering for Gray and Color Images", IEEE INTERNATIONAL CONFERENCE ON COMPUTER VISION, 1998
VINCENT; SOILLE: "Watersheds in Digital Spaces: An Efficient Algorithm Based on Immersion Simulations", IEEE TRANSACTIONS ON PATTERN ANALYSIS AND MACHINE INTELLIGENCE, vol. 13, no. 6, June 1991 (1991-06-01), XP000949356, DOI: doi:10.1109/34.87344
VINCENT; SOILLE: "Watersheds in Digital Spaces: An Efficient Algorithm Based on Immersion Simulations", IEEE TRANSACTIONS ON PATTERN ANALYSIS AND MACHINE INTELLIGENCE, vol. 13, no. 6, June 1991 (1991-06-01), XP000949356, DOI: doi:10.1109/34.87344
Attorney, Agent or Firm:
Sakai, Hiroaki (Kasumigaseki Building 2-5, Kasumigaseki
3-chome, Chiyoda-k, Tokyo 19, JP)
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