Title:
CELL PEELING DEVICE AND CELL PEELING METHOD
Document Type and Number:
WIPO Patent Application WO/2023/248527
Kind Code:
A1
Abstract:
The present invention addresses the problem of obtaining a cell peeling device and a cell peeling method that can quickly peel a cell aggregate from a culture container in a non-contact manner. A cell peeling device 10 is for peeling a cultured cell aggregate 1 from a culture container 2 to which the cell aggregate is adhered, and comprises: an ultrasonic probe 12 that irradiates the cell aggregate from outside the culture container 2 with an ultrasonic beam 20 having a beam diameter which is smaller than the size of the cell aggregate; a movement mechanism unit 14 that makes it possible to change the relative positions of the culture container and the ultrasonic probe; an edge recognition unit 18 that is capable of recognizing an edge of the cell aggregate; and a control unit 19 that calculates the trajectory for moving the ultrasonic probe from the edge of the cell aggregate and the output value of the ultrasonic beam at the position on the trajectory and controls the movement mechanism unit and the ultrasonic probe.
Inventors:
SASAKI KOTA (JP)
KOURAI YUUSUKE (JP)
SUZUKI MUTSUMI (JP)
KOURAI YUUSUKE (JP)
SUZUKI MUTSUMI (JP)
Application Number:
PCT/JP2023/005095
Publication Date:
December 28, 2023
Filing Date:
February 15, 2023
Export Citation:
Assignee:
HITACHI LTD (JP)
International Classes:
C12M3/00; C12M1/42
Domestic Patent References:
| WO2019044804A1 | 2019-03-07 | |||
| WO2016047368A1 | 2016-03-31 |
Foreign References:
| JP2014018185A | 2014-02-03 | |||
| JP2016158724A | 2016-09-05 |
Other References:
IMASHIRO CHIKAHIRO, HIRANO MAKOTO, MORIKURA TAKASHI, FUKUMA YUKI, OHNUMA KIYOSHI, KURASHINA YUTA, MIYATA SHOGO, TAKEMURA KENJIRO: "Detachment of cell sheets from clinically ubiquitous cell culture vessels by ultrasonic vibration", SCIENTIFIC REPORTS, vol. 10, no. 1, XP093075118, DOI: 10.1038/s41598-020-66375-1
YUTA KURASHINA, KENJIRO TAKEMURA: "Cell sheet Generation Employed by Acoustic Radiation Pressure by Bolt-clamped Langevin Transducer", MURATA SCIENCE FOUNDATION ANNUAL REPORT, JP, vol. 32, JP, pages 248 - 250, XP009551731, Retrieved from the Internet
YUTA KURASHINA, KENJIRO TAKEMURA: "Cell sheet Generation Employed by Acoustic Radiation Pressure by Bolt-clamped Langevin Transducer", MURATA SCIENCE FOUNDATION ANNUAL REPORT, JP, vol. 32, JP, pages 248 - 250, XP009551731, Retrieved from the Internet
Attorney, Agent or Firm:
HIRAKI & ASSOCIATES (JP)
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