CELL RECOVERY METHOD AND CELL CULTURE DEVICE

WIPO Patent Application WO/2020/255930

A1

The purpose of the present invention is to provide a cell recovery method that does not require a centrifuge and suppresses damage to cells. The present invention: is for recovering cells that have been cultured in a vessel that contains a liquid culture medium and have stuck to an inside surface of the vessel; and involves a culture medium draining step (step S11) for draining the culture medium from the vessel, a detachment solution supply step (step S12) for supplying a detachment solution to the vessel after the culture medium has been drained, the detachment solution being for detaching the cells from the inside surface of the vessel, a detachment solution draining step (step S14) for draining the detachment solution from the vessel before the cells are completely detached from the inside surface of the vessel, a waiting step (step S15) for waiting, after the detachment solution has been drained, until the cells detach due to the action of residual detachment solution, and a recovery solution supply step (step S16) for supplying a recovery solution to the vessel after the waiting step is complete, the recovery solution being for recovering the cells.

JP6745881	Digital microfluidic system for isolation of single cells and characterization of analytes
WO/2015/001321	BIOREACTOR CONSUMABLE UNITS

HORII DAICHI (JP)
TAKEUCHI HARUKI (JP)
SUDA YOSHIMASA (JP)
URABE YUJI (JP)

PCT/JP2020/023462

December 24, 2020

June 15, 2020

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SINFONIA TECHNOLOGY CO LTD (JP)

C12M1/32; C12M3/00; C12N1/02; C12N5/00

WO2012002497A1

2012-01-05

JP2008079554A	2008-04-10
JP2017006058A	2017-01-12
JP2011103832A	2011-06-02
JP2003235541A	2003-08-26
JP2017201946A	2017-11-16
JP2017006058A	2017-01-12

See also references of EP 3988639A4

KAJI, SUHARA & ASSOCIATES (JP)

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